User`s guide

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Lab-on-a-chip technology means downsizing of analytical techniques from lab-scale to
chip-scale:
• using techniques like electrophoresis, chromatography, and sieving.
• with fluorescence, absorbance, and MS detection.
• with a higher degree of automation, integrating multiple steps of a complex protocol
into a miniaturized system.
Virtually any biochemical testing that can be done in a laboratory can theoretically be
done on a chip.
Ladder
Each electrophoretic LabChip Reagent kit contains a ladder. A ladder contains DNA, RNA
fragments or proteins of known sizes and concentrations.
A ladder well is located at the bottom right of the chip. The ladder is analyzed first before
sample analysis takes place.
The peak sizes and markers defined for the ladder are assigned consecutively, starting
with the first peak detected in the ladder. Peaks appearing above the upper marker do not
have to be detected. The peak table for the ladder well shows the peak size and
concentration.
Lower Marker
An internal standard that is added to a sample in a well to assist in determining size of
the sample. The lower marker is the same as the first peak found in the DNA ladder.