User`s guide

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• Protect dye and gel-dye mix from light. Remove light covers only when pipetting. Dye
decomposes when exposed to light.
• Use a new syringe and electrode cleaner with each new LabChip kit.
• Do not touch the Agilent 2100 bioanalyzer during a chip run and never place it on a
vibrating ground.
• Keep all reagents and reagent mixes refrigerated at 4 °C when not in use.
• Allow all reagents and samples to equilibrate to room temperature for 30 minutes
before use.
• Use loaded chips within 5 minutes. Reagents might evaporate, leading to poor results.
RNA Assays:
• Always wear gloves when handling RNA, and use RNase-free tips, microfuge tubes and
water.
• It is recommended to denature all RNA samples and RNA ladder by heat before use
(70 °C, 2 minutes).
• Always vortex the dye concentrate for 10 seconds before preparing the gel-dye mix.