User guide

10
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
Chapter1 About the Products
Product information
How to use the
core kits and
adaptors
This user guide describes how to use the Library Builder
™
Fragment Core Kit for 5500
SOLiD
™
with the 5500 SOLiD
™
Fragment Library Standard Adaptors or the 5500
SOLiD™ Fragment Library Barcoding Adaptors. Use the 5500 SOLiD
™
48 Fragment
Library Core Kit with the adaptors for automated liquid-handling systems such as the
Beckman Coulter Biomek
®
FX
p
and Tecan Freedom EVO
®
instruments. To use the
5500 SOLiD
™
Fragment Library Core Kit with the adaptors, refer to Fragment Library
Preparation: 5500 Series SOLiD
™
Systems User Guide (Part no. 4460960).
Used with a wide range of barcoded adaptors, the Library Builder
™
Fragment Core Kit
for 5500 SOLiD
™
contains reagents and a protocol card to prepare fragment libraries
(100–250 bp, before adaptor ligation) or express libraries (100–550 bp, before adaptor
ligation). The protocol card directs the instrument to end-repair, ligate, and size-select
libraries.
After automated library preparation, you nick-translate and quantitate the library for
templated bead preparation on the Applied Biosystems SOLiD
™
EZ Bead
™
System
[refer to SOLiD
™
EZ Bead
™
Emulsifier Getting Started Guide (Part no. 4441486)].
5500 Series SOLiD™ System
Library Core Kits
5500 SOLiD™ Fragment Library Core Kit
(4464412)
5500 SOLiD™ 48 Fragment Library Core Kit
(4464415)
Library Builder™ Fragment Core Kit for 5500 SOLiD™
(4463763)
Adaptors
5500 SOLiD™ Fragment Library Standard Adaptors
(4464411)
5500 SOLiD™ Fragment Library Barcode Adaptors
(4464404)
SOLiD™ 4 System
Library Kits
SOLiD™ Fragment Library Construction Kit
(4443473)
Library Builder™ Fragment Core Kit for SOLiD™ 4.0
(4463762)
Adaptors
SOLiD™ Fragment Library Oligos Kit
(4401151)
SOLiD™ Fragment Library Barcoding Kit 1-96
(4449637)