User guide

11
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
Chapter1 About the Products
Kit contents and storage conditions
Kit contents and storage conditions
Kit contents
The Library Builder
™
Fragment Core Kit for 5500 SOLiD
™
(Part no. 4463763) contains
materials sufficient to prepare up to 13 standard or express fragment libraries:
The adaptors that are required to prepare fragment libraries with the AB Library
Builder
™
System are sold separately:
Part Description Storage temperature
AB Library Builder
™
Fragment
Reagents Module for 5500 SOLiD
™
• 13 cartridges. Each
cartridge contains
ready-to-use
reagents.
•13 tubes of 5✕
Reaction Buffer
tubes.
• 1 tube of Shear
Buffer.
•5 tubes of
Platinum
®
PCR
Amplification mix
and 1 tube each of
Library PCR
Primer 1 and Library
PCR Primer 2
–20°C
AB Library Builder
™
Plastics Module Sample and elution
tubes, tips, and tip
holders.
Room temperature
Part Description Storage temperature
5500 SOLiD
™
Fragment Library
Standard Adaptors (4464411)
One each –20°C
5500 SOLiD
™
Fragment Library
Barcode Adaptors (4464404)
One each –20°C