User guide

13
Fragment Library Preparation Using the AB Library Builder
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System: 5500 Series SOLiD
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Systems User Guide
CHAPTER2
Prepare to Build the Library
Workflow
Procedural guidelines
• The protocol is designed for 10 ng–5 µg of genomic DNA or ligated PCR product.
• Use good laboratory practices to minimize cross-contamination of products.
• Adjust microcentrifuge speeds and times according to the g-forces specified in the
protocols. Applied Biosystems recommends the Eppendorf 5417R tabletop
microcentrifuge.
• Perform all steps requiring 0.5-mL and 1.5-mL tubes with 0.5-mL Eppendorf
LoBind Tubes (Eppendorf Part no. 022431005) and 1.5-mL Eppendorf LoBind
Tubes (Eppendorf Part no. 022431021).
• Thaw reagents on ice or at room temperature before use, but thaw Shear Buffer at
room temperature.
Quantitate the DNA (page 14)
Shear the DNA (page 14)
Set up the AB Library Builder
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System for size-selected or express fragment
library preparation (page 17)
Inspect the AB Library Builder
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Cartridges (page 17)
Insert or change the protocol card and power ON the instrument (page 18)
Load the racks and tubes (page 19)
Insert the racks into the AB Library Builder
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Device (page 23)