User guide
17
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
Chapter2 Prepare to Build the Library
Set up the AB Library Builder
™
System for size-selected or express fragment library preparation
STOPPING POINT Store the DNA in a supplied Sample Tube at 4°C for short-term
storage or at –20°C for long-term storage, or proceed directly to “Set up the
AB Library Builder
™
System for size-selected or express fragment library
preparation”.
Set up the AB Library Builder
™
System for size-selected or
express fragment library preparation
About size-
selected and
express fragment
library preparation
AB Library Builder
™
System prepares standard (size-selected) or express (not size-
selected) DNA fragment libraries. Use the AB Library Builder
™
System with the
Library Builder
™
Fragment Core Kit for SOLiD
™
5500 and Agencourt AMPure
®
XP
Reagent to end-repair, size-select (optional), ligate, and purify fragment libraries.
To install and set up the AB Library Builder
™
System the AB Library Builder
™
System
Site Preparation Guide (Part no. 4465106) and the AB Library Builder
™
System User Guide
(Part no. 4463421).
IMPORTANT! To avoid data loss or run cancellation, always follow these practices:
• Before you insert or remove a protocol card, power the instrument OFF
• Before you power the instrument ON, insert the protocol card, then close the
instrument door.
• To pause the instrument during an extraction run, press Stop before you open the
instrument door
• When you are not performing a run or instrument test, you can open the instrument
door with the power OFF or ON
• Do not move instrument components such as the platform, magnets, and syringes
while the instrument is powered ON.
Before a run, follow these procedures to set up the instrument:
1. “Inspect the AB Library Builder
™
Cartridges”.
2. “Insert or change the protocol card and power ON the instrument” on page 18.
3. “Load the racks and tubes” on page 19.
4. “Insert the racks into the AB Library Builder
™
Device” on page 23.
Inspect the AB
Library Builder
™
Cartridges
Each cartridge has 12 compartments for reagents: