User guide

18
Fragment Library Preparation Using the AB Library Builder
System: 5500 Series SOLiD
Systems User Guide
Chapter2 Prepare to Build the Library
Set up the AB Library Builder
System for size-selected or express fragment library preparation
Insert or change
the protocol card
and power ON the
instrument
IMPORTANT! Do not remove the protocol card while the instrument is on. Removing
the card stops the run, and it may cause instrument data file loss. To remove the card,
see step 3.
If you accidentally remove the protocol card during a run, power off the instrument
immediately to minimize potential for instrument data loss.
For guidelines on handling protocol cards, see the AB Library Builder
System User
Guide (Part no. 4463421).
1. Confirm that the power switch is in the OFF position.
Note: If you insert the card while the instrument is on, the instrument does not
recognize the card.
2. Open the card slot.
Cartridge compartment numbers Volumes
1 1300 µL
2–3 1200 µL
4 Empty
5–6 20 µL
7 85 µL
890µL
9–10 20 µL
11: Tube to be added after 5 Reaction
Buffer tube prepared
42.5 µL
(final volume
is 85 µL)
12: Unsealed compartment for beads