User guide

19
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
Chapter2 Prepare to Build the Library
Set up the AB Library Builder
™
System for size-selected or express fragment library preparation
3.
To remove a card that is already in the slot, push the button located below the
card slot (see the photo below), then pull the card out of the slot. Place the card in
the plastic cover in the box.
IMPORTANT! Do not remove the protocol card while the instrument is on.
4. Insert the appropriate protocol card in the slot with the arrow on the protocol
card pointing toward the instrument and the label facing left.
5. Push the card completely into the card slot, then close the card slot.
6. Close the door to the AB Library Builder
™
Device.
7. Power ON the instrument.
When the card is fully inserted in the correct orientation, the display briefly
shows information including the instrument version, then displays the Main
menu.
8. Press START.
Load the racks and
tubes
Note: To ensure the best pipetting performance, use the cartridge rack and tip and
tube rack shipped with the instrument; these racks are calibrated with the instrument
at the factory. Before using other racks on a specific instrument, run the installation test
to qualify the racks for use on that instrument. Refer to the AB Library Builder
™
System
User Guide for details
Wear gloves when you handle samples or load the cartridges, tips, and tubes in the
rack.
Remove the racks from the instrument
Open the instrument door (push up the door), then remove the tip and tube rack and
the cartridge rack: