User guide
23
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
Chapter2 Prepare to Build the Library
Set up the AB Library Builder
™
System for size-selected or express fragment library preparation
3.
Row E (first row): Load with labeled sample/elution tubes, with the caps removed
and secured:
Insert the racks
into the AB Library
Builder
™
Device
IMPORTANT!
• Insert the cartridge rack before the tip and tube rack. Changing the order of
loading the racks may cause the instrument to stop during a run.
• Use only AB Library Builder™ Sample Tubes (sample/elution tubes). Other tubes
may be picked up by the nozzle tips due to differences in tube height and shape,
stopping the run.
Insert the cartridge rack
IMPORTANT! Before inserting the cartridge rack into AB Library Builder
™
Device,
ensure that the cartridges are completely thawed, particularly reagents in cartridge
wells 2 and 3.
S-Sample tube
T2-Tip and tip holder
T1-Tip and tip holder
E-Elution
(Optional) Place elution tube caps here