User guide
27
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
CHAPTER3
Build the library
Workflow
For additional instructions on instrument operation, see “AB Library Builder
™
System
operation” on page 48.
Start the run
1. Press START to select the AB Library Builder
™
System Kit option.
2. Confirm that you have loaded and inserted the cartridge rack and tip and tube
rack correctly.
3. Select the script for the kit you are using, then follow the on-screen prompts.
4. (Optional) Scan the sample, elution tube, and sample lane barcodes [refer to the
AB Library Builder
™
System User Guide (Part no. 4463421)].
5. Close the door to the AB Library Builder
™
Device.
6. Press START.
The screen shows the current step and the approximate incubation time
remaining.
IMPORTANT! Do not open the door during a protocol run. To pause or cancel the
run, see “AB Library Builder
™
System operation” on page 48.
Note: If you lose power or the power cord is unplugged, the run stops. When the
power resumes, the digital display shows the Main menu. You cannot resume the
run. If the tips are still on the syringe unit when the power resumes, return the
tips to the original positions as described in “AB Library Builder
™
System
operation” on page 48.
7. At the end of the run (the instrument beeps briefly and the digital display shows
“Finished Protocol”). To unload the instrument:
a. Press to return to the Main menu, then open the instrument door.
Start the run (page 27) (run time: ~2.5 h)
Set up for a new run (page 28)