User guide

35
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
CHAPTER5
Troubleshooting
For symptoms other than those listed in this section, contact Technical Support
(“Obtaining support” on page 81).
Observation Possible Cause Recommended action
Before loading the cartridges in the cartridge rack
Precipitate in AB
Library Builder
™
5✕ Reaction Buffer
tubes
5✕ Reaction Buffer tubes
were exposed to low
temperatures during
shipping or storage.
To dissolve precipitate that may have formed during shipping or storage,
incubate the 5✕ Reaction Buffer tubes at 37°C for 5 minutes or until
precipitate is no longer visible.
During the automated run
No power (the
digital display is
blank and the fan
does not turn on
when you power
on)
AC power cord is not
connected
Check AC power cord connections at both ends. Use the correct cords.
Fuse has blown Check the integrity of the fuse and replace it if necessary (refer to the
AB Library Builder
™
System User Guide).
If the problem persists after connecting the correct power cord and
replacing the fuse, contact Technical Support (“Obtaining support” on
page 81).
The digital display
is blank, but the fan
turns on when you
power on.
Protocol card is not
inserted correctly
Power off the instrument and re-insert the protocol card in the proper
orientation into the card slot (see “Insert or change the protocol card
and power ON the instrument” on page 18). Insert it completely into the
slot by manually pushing the card.
Protocol card was
inserted when the
instrument was powered
on
Power off the instrument, then power on the instrument.
Error code
displayed
— See “Instrument error codes” on page 38.
Reagent
cartridges, tips, or
tubes are not
inserted in the
correct positions.
— Press STOP to pause the run. Open the door, add the missing items,
then press START to resume the run. Do not open the door without
pausing the run.