User guide

36
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
Chapter5 Troubleshooting
Run stops after an
initial start (you
may also see an
error code).
• Instrument door
opened during the run
• Reagent cartridges,
tips, or tubes
incorrectly loaded in
the rack
•Racks incorrectly
loaded on the
instrument
IMPORTANT!
If you open the instrument door while the instrument is running, the run
stops, and it cannot be restarted. If you need to open the instrument
door during a run, first press Stop to pause the run, then open the door.
1. Follow the procedure in “Instrument error codes” on page 38.
2. Before starting a new run, make sure that the reagent cartridges,
tips, and tubes are correctly loaded:
• Slide the reagent cartridges into the cartridge rack as described
in “Load the racks and tubes” on page 19.
• Load the cartridge rack before the tip and tube rack for proper
positioning.
• Do not cap the tubes.
3. If the instrument continues to stop during the run, contact Applied
Biosystems Technical Support.
Reagent cartridges not
completely thawed
1. Stop the run.
2. Remove the tip and tube rack, then remove the cartridge rack.
3. Inspect cartridge wells 2 and 3 for ice.
4. If any well is frozen, close the door to the AB Library Builder
™
Device, then thaw the cartridges completely.
5. Replace the tips in position T2.
6. Insert the cartridge rack then the tip and tube rack onto the
AB Library Builder
™
Device.
7. Restart the run.
No DNA yield No sample added to tube Add samples to tubes, load new reagent cartridges, then perform the
run again.
No liquid in tip, or
liquid in tip not
moving
No sample added to
tube, leading to wet filter
barrier on the tip and
blockage of nozzles
Add samples to tubes, load new reagent cartridges, then perform the
run again.
Buffer in the
bottom tray
Motor movements are
not smooth
Schedule preventive maintenance annually to ensure proper motor
movements.
Reagent cartridges, tips,
or tubes incorrectly
loaded in the rack
If you are processing fewer than 13 samples, make sure to load the tips
and tubes in the same positions as the reagent cartridges that are
loaded in the cartridge rack.
See below for leakage from tips.
Leakage from tips
or uneven liquid
handling between
nozzles
D-Rings are not greased
regularly or they need
replacement
You can continue the run, but maintain the D-rings as scheduled. To
prevent leakage, maintain or replace the D-rings (refer to the (AB
Library Builder
™
System User Guide).
Blockage of tips Too much starting
material causing clumps
or aggregates
Contact Technical Support (“Obtaining support” on page 81).
In future runs, use the sample volume recommended in the user guide
for the kit you are using.
Observation Possible Cause Recommended action