User guide

39
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
A
APPENDIXA
Ordering Information
This appendix covers materials for barcoded fragment library preparation:
■ Required Applied Biosystems reagent kits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
■ Required equipment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
■ Optional equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
■ Replacement parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
■ Required consumables. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
■ Optional consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Sufficient reagents are supplied in the AB Library Builder
™
System Kit to prepare 13
libraries for high-throughput sequencing with the 5500 Series SOLiD
™
System.
Upon receipt of the AB Library Builder
™
System Kit, immediately store each
components at the temperature specified on the label.
Required Applied Biosystems reagent kits
Item (part number)
†
† Applied Biosystems has validated this protocol using this specific material. Substitution
may adversely affect system performance.
Components
Library Builder
™
Fragment Core Kit for 5500
SOLiD
™
(4463763)
• AB Library Builder
™
Reagents Module for
5500 SOLiD
™
• AB Library Builder
™
Plastics Module
Item
†
Source
5500 SOLiD
™
Fragment Library Standard
Adaptors (4464411)
• Barcode-T-001, 50 µM
•P1-T Adaptor, 50µM
• Library PCR Primer 1,
50 µM
• Library PCR Primer 2,
50 µM