User guide

43
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
AppendixA Ordering Information
Required equipment
A
Covaris
®
S220 System
‡
(110 V for U.S. customers)
(220 V for international customers)
The Covaris
®
S220 System includes:
•Covaris
®
S220 sonicator
•Universal Voltage Kit
•Latitude
®
laptop from Dell
®
Inc.
• MultiTemp III Thermostatic Circulator
•Covaris
®
-2 series Machine Holder for
(one) 1.5-mL microcentrifuge tube
•Covaris
®
-2 series Machine Holder for
(one) 0.65-mL microcentrifuge tube
•Covaris
®
-2 series Machine Holder for
(one)
13 mm × 65 mm tube
•Covaris
®
-2 Series Machine Holder for
(one) microTUBE
•Covaris
®
microTUBE Prep Station
•Covaris
®
Water Tank Label Kit
•Covaris
®
microTUBEs (1 pack of 25)
Applied Biosystems
4465653
Covaris
®
S2 System
§
(110 V for U.S. customers)
(220 V for international customers)
Note: Fragment libraries
can be prepared with the
Covaris
®
S2 System. New
users should purchase the
Covaris
®
S220 System.
Microcentrifuge 5417R, refrigerated, without
rotor
• Eppendorf
††
022621807 (120 V/60
Hz)
• Eppendorf
‡
022621840 (230 V/50
Hz)
FA-45-24-11, fixed-angle rotor,
24 × 1.5/2 mL, including aluminum lid,
aerosol-tight
Eppendorf
‡
022636006
96-well GeneAmp
®
PCR System 9700
(thermal cycler)
• Applied Biosystems
N8050200 (Base)
• Applied Biosystems
4314443 (Block)
‡
NanoDrop
®
ND-1000 Spectrophotometer
(computer required)
Thermo Scientific
ND-1000
E-Gel
®
iBase
™
and E-Gel
®
Safe Imager
™
Combo Kit
Invitrogen
G6465
Item
†
Source