User guide

44
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
AppendixA Ordering Information
Optional equipment
A
Optional equipment
Replacement parts
DynaMag
™
- 2 Magnet (magnetic rack) Invitrogen
123-21D
Vortexer Major Laboratory Supplier
(MLS)
Picofuge MLS
Pipettors, 2 µL MLS
Pipettors, 20 µL MLS
Pipettors, 200 µL MLS
Pipettors, 1000 µL MLS
† Applied Biosystems has validated this protocol using this specific material. Substitution
may adversely affect system performance.
‡ Or the Covaris
®
S2 System.
§ Or the Covaris
®
S220 System.
††Or equivalent but validation of the equipment for library preparation is required.
Item
†
Source
Item
†
† Applied Biosystems has validated this protocol using this specific material.
Substitution may adversely affect system performance.
Source
E-Gel
®
iBase
™
and E-Gel
®
Safe Imager
™
Combo Kit
Invitrogen
G6465
2100 Bioanalyzer
™
Agilent Technologies
G2938C
Qubit
™
Quantitation Starter Kit Invitrogen
Q32860
Qubit
®
2.0 Fluorometer Invitrogen
Q32866
Product name
†
Vendor
AB Library Builder
™
Tips and Tip Holders 4463781
AB Library Builder
™
and Tube Rack 4463776
AB Library Builder
™
Cartridge Rack 4463782
AB Library Builder
™
D-Ring Tool 4465603
AB Library Builder
™
Barcode Reader 4465657
AB Library Builder
™
Sample Tubes 4463779