User guide

46
Fragment Library Preparation Using the AB Library Builder
System: 5500 Series SOLiD
Systems User Guide
AppendixA Ordering Information
Optional consumables
A
Optional consumables
Ethylene glycol American Bioanalytical
AB00455-01000
0.5-mL LoBind Tubes Eppendorf
022431005
1.5-mL LoBind Tubes Eppendorf
022431021
Filtered pipettor tips Major Laboratory
Supplier (MLS)
Applied Biosystems has validated this protocol using this specific material.
Substitution may adversely affect system performance.
For the SDS of any chemical not distributed by Applied Biosystems, contact the
chemical manufacturer. Before handling any chemicals, refer to the SDS provided by
the manufacturer, and observe all relevant precautions.
Item
Source
Product name
Applied Biosystems has validated this protocol using this specific material.
Substitution may adversely affect system performance.
Vendor
50-bp ladder Invitrogen
10416-014
SOLiD
Library Size Selection Gel Applied Biosystems
4443733
CF-1 Calibration Fluid Kit Thermo Scientific
CF-1
PR-1 Conditioning Kit
‡The NanoDrop
®
Conditioning Kit is useful for “reconditioning” the sample
measurement pedestals to a hydrophobic state if they become “unconditioned” (refer
to the Nanodrop
®
Conditioning Kit user’s manual for more information). The PR-1 kit
consists of a container of specially formulated polishing compound and a supply of
convenient applicators.
Thermo Scientific
PR-1
Agilent DNA 1000 Kit Agilent Technologies
5067-1504