User guide
52
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
AppendixB Supplemental Procedures
(Optional) Size-select and pool libraries
B
Load the gel
For exact fill volumes of the wells, refer to the Invitrogen E-Gel
®
SizeSelect
™
Agarose Gels
Quick Reference Card.
1. Load 16 µL (≤ 1 µg/lane) of the (pooled) library DNA into wells 2, 3, 6 or 7 of the
top row of wells. If the sample volume is < 20 µL, add Nuclease-free Water to the
well for a total volume of 20 µL. Skip the center well (smaller well in the top
center of the gel for the ladder); and skip a single well to the right and left of the
center top well. Skip the two outermost wells (to avoid edge effects). Do not load
more than 1 µg of DNA per lane.
2. Load 10 µL of 50-bp ladder at 0.1 µg/µL to the center top well. Add 7 µL of water
to fill the well.
3. Fill the empty wells in the top row with 20 µL of Nuclease-free Water.
4. Fill each of the collection wells in the middle of the gel with 25 µL of Nuclease-free
Water. Add 20 µL of Nuclease-free Water to the middle center well.