User guide

53
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
AppendixB Supplemental Procedures
(Optional) Size-select and pool libraries
B
The following figure shows you where to load DNA, ladder, and Nuclease-free
Water on a SOLiD™ Library Size Selection gel to size-select the DNA (“M” is the
middle well for the ladder):
Run the SOLiD
™
Library Size Selection Gel
1.
Run the gel:
• iBase system program: SizeSelect 2%
• Run time: 14:30 (14 minutes and 30 seconds)
Monitor the SOLiD
™
Library Size Selection gel in real-time with the E-Gel
®
Safe
Imager Real-Time Transilluminator.
2. During the run, fill the middle collection wells with additional Nuclease-free
Water to ensure optimal migration of the DNA through the wells.
DNA
DNALadder
Nuclease-
free
Water
Nuclease-
free
Water
Nuclease-
free
Water
Nuclease-
free
Water
25 μL
Nuclease-
free
Water
20 μL
Nuclease-
free
Water
25 μL
Nuclease-
free
Water