User guide
57
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
AppendixB Supplemental Procedures
Quantitate the DNA with the NanoDrop
®
ND-1000 Spectrophotometer
B
4.
Lift the sampling arm and load 2 µL of Nuclease-free Water onto the lower
measurement pedestal and lower the sampling arm:
5. In the dialog box, click OK and allow the instrument to initialize.
6. Lift the sampling arm and use Kimwipes
®
to remove water from the
measurement pedestal and the sampling arm.
7. Load 2 µL of the same buffer that was used to resuspend or elute the DNA onto
the measurement pedestal and lower the sampling arm.
8. Click Blank and allow the instrument to take a measurement:
9. Lift the sampling arm and wipe away the buffer from both the upper and lower
measurement pedestals with Kimwipes
®
. The instrument is now ready to take
readings.
10. Load 2 µL of DNA sample onto the lower measurement pedestal and lower the
sampling arm.