User guide

62
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
AppendixC Supplemental Background Information
Sequence orientation from source DNA to sequence map
C
Sequence orientation from source DNA to sequence map
For more information on sequencing tags, refer to 5500 Series SOLiD
™
Sequencers User
Guide (Part no. 4456991).
Sheared,
size-selected DNA
Circularization with
Internal Adaptor and
Nick Translation
Nick-translation goes
beyond the length of the read.
Sequencing of the
fragment on a bead
Sequencing of the
fragment on a bead
F3 R3
F3 R3
P1 P2IA
F3
BCF5
P1 P2IA
IA
IA
F3
R3
F3
R3
Sheared,
size-selected DNA
F3 F5
ǩ5HDGVDUHJHQHUDWHGIURP)DQG5SULPHUV5PDSVXSVWUHDPIURP)1RWHWKDW
5PDSVXSVWUHDPRI)LQWKHVRXUFH'1$IUDJPHQW
ǩ 7KHUHDGVDUHH[SHFWHGWRPDSDWDGLVWDQFHHTXDOWRWKHDYHUDJHIUDJPHQWOHQJWK
SOXVWKHDYHUDJHQLFNWUDQVODWLRQGLVWDQFH
ǩ7KHVHTXHQFLQJGLUHFWLRQIRUmate-pair libraries is 5′ to 3′.
ǩ 7KHVHTXHQFLQJGLUHFWLRQIRU)UHDGVRIpaired-end libraries is 5′ to 3′.
ǩ 7KHVHTXHQFLQJGLUHFWLRQIRU)UHDGVRIpaired-end libraries is 3′ to 5′7RVXSSRUW
traditional 5′ to 3′UHSUHVHQWDWLRQWKHFRPSOHPHQWRIWKHUHDGVDUHZULWWHQ
Mate-pair
Paired-end