User guide

85
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
Index
A
AB Library Builder System
running 27
AB Library Builder™ Kits
contents 9
, 10
about the kit 9
B
barcoded fragment library preparation 9, 13, 27, 29,
35
biohazardous waste, handling 80
build the library 27
C
cartridge rack
insert in instrument 24
load cartridges 19
CAUTION, description 7
change protocol card 18
checklists and workflow tracking forms 73
chemical hazard warning 77
chemical safety 77
, 78
chemical waste safety 78
, 79
cooling period, not required 28
Covaris microTUBE holder, vials, system,
programs 47
D
DANGER, description 7
digital display
description 49
troubleshooting 35
DNA yield, troubleshooting 36
documentation, related 81
E
elution volume, troubleshooting 36, 37
error codes
list of 38
troubleshooting 35
G
glossary 83
guidelines
chemical safety 78
chemical waste disposal 78
chemical waste safety 79
H
hazard warning, chemical 77
hazards. See safety
I
IMPORTANT, description 7
insert protocol card 18
instrument
cancel run 50
display 49
error codes 38
pause run 50
start run 27
troubleshooting 35
K
kit contents 10
L
leakage, troubleshooting 36
M
MSDS. See SDS
multiple fragment libraries
(optional) amplify the libraries 31
quantitate the DNA 33
shear the DNA 14
, 33