User guide

9
Fragment Library Preparation Using the AB Library Builder
System: 5500 Series SOLiD
Systems User Guide
CHAPTER1
About the Products
Note: For complete site preparation and operating instructions of the AB Library
Builder
System, refer to the AB Library Builder
System Site Preparation Guide (Part
no. 4465106) and the AB Library Builder
System User Guide (Part no. 4463421) at:
http://www.appliedbiosystems.com/librarybuilderguides.
For a more detailed overview of library types and the library preparation workflows,
see “Supplemental Background Information” on page 59.
Library preparation
Library preparation is the first step in which samples are adapted for sequencing on
the 5500 Series SOLiD
Sequencers. During library preparation, forward and reverse
adaptors are added to the ends of DNA inserts (The bead is for illustration purposes
only and is not added until the bead preparation step):
Product information
Purpose of the
products
To prepare fragment and barcoded fragment libraries for sequencing on the
5500 Series SOLiD
Sequencers, Life Technologies offers a system of kits and adaptors
to customize preparation of single to multiplexed, barcoded libraries (Life
Technologies part numbers are in parentheses. For comparison, the SOLiD
4 System
kits and adaptors are shown):