User`s guide

Additional Imaging Modes 7
Agilent 5500 SPM User’s Guide 127
12
In the Scan and Motor window’s Scan tab, enter:
a Scan Speed of 1-2 ln/s.
b Resolution of 256.
c Scan Size (in microns).
d X Offset and/or Y Offset values to set the center of the scan.
13 In the Realtime Images window, choose to display Topography,
CSAFM/BNC Aux (Amplitude) and PicoPlus Aux (Phase). If using
a MAC III controller select Aux 1 and Aux 2.
14 In the Scan and Motor window, click the Up blue arrow to initiate a
scan starting at the bottom of the grid. Click the Down blue arrow to
initiate the scan from the top down. The image maps will begin to be
rendered in the Realtime images window.
Force Modulation Microscopy (FMM)
Force Modulation is another derivative of Contact Mode, with
similarities to AC Mode as well. In FM Mode, an additional 20-50 kHz
oscillation is applied to the cantilever. The amplitude and phase of
oscillation will change depending upon the modulus of the surface at
any given point.
The multi-purpose scanner and AAC nose assembly are used for FM
Mode. A specific Force Modulation cantilever is available through
Agilent; however, the best choice of cantilever is often experimentally
determined. A MAC Mode or MAC III controller is also required.
With the MAC Mode controller the following cables must be added:
• Connect a BNC cable from the Phase output of the MAC Mode
controller to the Aux In of AFM Controller.
• Connect a BNC cable from the Amplitude output of the MAC Mode
controller to the Aux BNC on the Head Electronics Box.