User`s guide

Temperature Control 13
Agilent 5500 SPM User’s Guide 200
Note that Cable 3 includes a tab on the black-wire side of the connector
(Figure 134). The tab must face the Lo jack on the controller.
Figure 134 Tab side of Cable 3 must be inserted into the Lo jack.
Imaging
1 Set up the microscope for typical operation. As mentioned, uncoated
silicon probes are highly recommended.
2 Mount the sample on the sample plate. Do not use double-sided tape
to mount the sample because the glue may soften or melt, causing
large sample drift
.
3 Turn on the Lakeshore temperature controller.
4 On the Lakeshore controller’s front panel, press the Heater Off
button.
5 Press Auto Tune, then press the Up or Down arrow buttons until the
display read Tune:Manual.
6 Set the Proportional, Integral and Differential gains. Typical
values are 20, 20 and 100 respectively.
7 Press Setpoint and enter a value slightly lower than room
temperature (23 C). Wait for the setpoint value to stabilize.
8 Set the Ramp Rate to no more than 10 degrees per minute (5
degrees/minute is a typical ramp rate).
NOTE
When turned on, the Lakeshore controller will attempt to adjust the plate
temperature to the last selected temperature, as quickly as possible.
Depending on the sample, and the last temperature setting, this can be
detrimental to the plate and/or sample. Beginning with a setpoint slightly
below ambient avoids this problem.