User Guide

490 Series Microscope
AmScope Proprietary Page 31 of 64
4. If the bright ring is still obscure, raise or
lower the condenser by adjusting the
condenser focusing knob (or the
microscope focusing knob if necessary)
until the bright ring is in focus and the
dark ring is visible.
5. If the two ring images are not
positioned so one is inside the other,
adjust the centering screws on the
condenser ring plate until the two rings
center in parallel.
The annulus’ bright ring of
light should overlap the
dark grey ring, which is the
objective’s phase ring.
6. Place the green filter in the filter holder
or directly onto the center of the base
lens of your microscope (where the
light emerges from). Remove the
green filter if doing darkfield
microscopy.
7. Remove the centering telescope and replace the eyepiece.
Note: Once aligned, repeat the process for each magnification.