User Guide

490 Series Microscope
AmScope Proprietary Page 35 of 64
2. Place a slide on the microscope stage.
Make sure the specimen is covered with a
cover slip (a good example for a phase
contrast specimen is human saliva). Clean
any excess material from the slide.
3. Turn the phase contrast condenser’s turret to the B setting. Focus the
specimen. Before aligning the phase contrast lens and condenser, you will
need to bring the specimen into focus with the brightfield setting.
4. Once the specimen is in focus with the brightfield setting, rotate the
condenser’s turret ring so it is set to 10.
2.21.4 Calibrating the Turret Phase Contrast Objectives and Condenser Lenses
The turret has a built-in annulus for each
objective magnification. Once the turret
and objective lens are set with matching
numbers, they will need to be aligned.
You will need to do this again each time
you change the objective lens.
Each annulus in the turret is easy to
reposition by using a finger.
1. Remove one eyepiece from the
microscope eyetube and insert the
centering telescope.
2. Turn the microscope’s light on and
look through the telescope lens.