User Guide

490 Series Microscope
AmScope Proprietary Page 36 of 64
3. While looking through the telescope
lens, extend the eyepiece by rotating the
top counter clockwise.
4. Keep rotating the telescope lens until
an image of the objective’s phase ring
and the condenser’s annulus are clearly
in focus.
5. By slowly moving the annulus on the
underside of the turret, now in-line with
the condenser’s lens, the annulus’ bright
ring of light should overlap the dark grey
ring, which is the objective’s phase ring
6. If the bright ring is still obscure, raise or
lower the condenser by adjusting the
condenser focusing knob (or the
microscope focusing knob if necessary)
until the bright ring is in focus and the
dark ring is visible.
7. Place the green filter in the filter holder
or directly onto the center of the base
lens of your microscope (where the
light emerges from). Remove the
green filter if doing darkfield
microscopy.
8. Remove the centering telescope and replace the eyepiece.
Note: Once aligned, repeat the process for each magnification.