User Guide

490 Series Microscope
AmScope Proprietary Page 37 of 64
2.21.5 Perform the Phase Contrast Observation
After you center the ring plate, you can perform the phase contrast observation
the same way as a normal brightfield microscope.
Note: When changing to another phase contrast objective and corresponding
condenser ring plate, the focusing and centering of bright ring and dark
ring should be repeated following the procedures above.
Tips:
1. Make the illumination as bright as possible.
2. The thinner the specimen, the better the image.