Operator’s Manual Rev C 1/14/15 2052 O’Neil Rd, Macedon NY 14502 Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.
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Table of Contents Introduction .................................................................................................................................................................. 5 Warranty ....................................................................................................................................................................... 5 Filter Bags .............................................................................................................................
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Operator’s Manual Introduction ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical laboratories around the world in the environmental, agricultural, biomass, and food industries. ANKOM Technology can provide you with products for determining or monitoring detergent fibers, dietary fibers, fat, digestibility, microbial fermentation (anaerobic or aerobic) and more.
Operator’s Manual Contact Information At ANKOM Technology we are committed to your total satisfaction and therefore always available to help you get the most from your ANKOM products. We are also very interested in any comments or suggestions you may have to help us improve. For any questions or suggestions regarding your instrument, please contact us at: pg. 6 Telephone: (315) 986-8090 Fax: (315) 986-8091 Email: service@ankom.com www.ankom.
Operator’s Manual Instrument Description General Description The ANKOM200 Fiber Analyzer is designed to efficiently and accurately determine Acid Detergent Fiber (ADF), Neutral Detergent Fiber (NDF), and Crude Fiber within food and/or feed samples. Enabled by Filter Bag Technology, up to 24 samples can be processed at one time.
Operator’s Manual Internal Components (Rear View) Packing Nut Maintenance Alert Collector (part # F27) External Components (Rear View) Maintenance Alert Reservoir pg.
Operator’s Manual Safety Precautions Hazardous Pressure – The contents of the Vessel are hot and under pressure. Do NOT open the Vessel Lid during or after an operation until both pressure and liquid are fully exhausted from the instrument. Connect and secure the Drain Hose along the path to the drain so it will not move when hot pressurized fluid is exhausted. Failure to observe this caution may result in uncontrolled chemical flow, scalding, or burning.
Operator’s Manual Instrument Installation Site Requirements To install and operate the ANKOM200 Fiber Analyzer you will need the following: Adjustable wrench Water supply located close to the ANKOM200 capable of heating water to 50°C for Crude Fiber and 70°C for ADF/NDF analyses Adequate power (see “Operating Environment” section) Drain Instrument Installation Procedure To install the ANKOM200 Fiber Analyzer, follow the procedure detailed below. 1.
Operator’s Manual Fiber Analysis Support Items The following support items are needed to perform the fiber analysis procedures: Item Recommended Product Electronic Balance with four-place readout ANKOM #TB Balance Hardware ANKOM #TBS Balance Software ANKOM #F57 ANKOM #101.
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Operator’s Manual ADF Analysis ADF Calculation ADF contained within a food or feed sample can be calculated using the following formula: % ADF (as-received basis) = 100 x (W3 – (W1 x C1)) W2 Where: = = = = Bag tare weight Sample weight Dried weight of filter bag with fiber after extraction process Blank bag correction (running average of final oven-dried weight divided by original blank bag weight) W1 W2 W3 C1 ADF Sample Preparation Procedure To prepare samples for fiber analysis, follow the procedu
Operator’s Manual 8. Record the weight of the sample (W2). 9. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of the filter bag. If the seal is not strong, re-seal the bag. 10. To eliminate sample clumping, spread the sample out uniformly within the filter bag by shaking and flicking the bag. 11.
Operator’s Manual ADF Analysis Procedure using the ANKOM200 Fiber Analyzer To perform the ADF analysis on prepared samples, follow the procedure detailed below. The Vessel must be at room temperature before beginning an ADF analysis. 1. Verify that the Exhaust Hose is connected to the instrument and securely positioned in the drain. 2. Turn the instrument Power Switch to the ON position. 3. Lift the Lid Handle to open the Vessel Lid. 4.
Operator’s Manual 17. Repeat steps 12 - 16 to accomplish the second rinse. 18. Make sure that the HEAT button is off (not lit) so that pH can be checked after the third rinse. 19. Repeat steps 12 - 16 to accomplish the third rinse. 20. After the third rinse, open the Vessel Lid and check that the water is pH neutral. If the water is NOT pH neutral, repeat steps 12 - 16 until the pH is neutral. 21. When the rinsing process is complete, remove the Bag Suspender from the Vessel. 22.
Operator’s Manual NDF Analysis NDF Calculation NDF contained within a food or feed sample can be calculated using the following formula: % NDF (as-received basis) = 100 x (W3 – (W1 x C1)) W2 Where: = = = = Bag tare weight Sample weight Dried weight of filter bag with fiber after extraction process Blank bag correction (running average of final oven-dried weight divided by original blank bag weight) W1 W2 W3 C1 NDF Sample Preparation Procedure To prepare samples for fiber analysis, follow the procedu
Operator’s Manual 8. Record the weight of the sample (W2). 9. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of the filter bag. If the seal is not strong, re-seal the bag. 10. To eliminate sample clumping, spread the sample out uniformly within the filter bag by shaking and flicking the bag. 11.
Operator’s Manual NDF Analysis Procedure using the ANKOM200 Fiber Analyzer To perform NDF analysis on prepared samples, follow the procedure detailed below. The Vessel must be at room temperature before beginning an NDF analysis. 1. Verify that the Exhaust Hose is connected to the instrument and securely positioned in the drain. 2. Turn the instrument Power Switch to the ON position. 3. Lift the Lid Handle to open the Vessel Lid. 4.
Operator’s Manual 18. After the third rinse, open the Vessel Lid and remove the Bag Suspender from the Vessel. 19. Remove the bags from the Bag Suspender trays and place them in a 250 ml beaker. 20. With your hands, gently press out excess water from the bags into the beaker and pour off the water from the beaker. 21. With the bags in a 250 ml beaker, add enough acetone to cover them. Let the bags soak in acetone for 3 - 5 minutes. Pour off the acetone. 22.
Operator’s Manual Crude Fiber Analysis Crude Fiber Calculation Crude Fiber contained within a food or feed sample can be calculated using the following formula: % Crude Fiber Where: = W1 W2 W3 C1 = = = = 100 x (W3 – (W1 x C1)) W2 Bag tare weight Sample weight Weight of Organic Matter (loss of weight on ignition of bag and fiber) Ash corrected blank bag factor (running average of loss of weight on ignition of blank bag / original blank bag) Crude Fiber Sample Preparation Procedure To prepare samples for
Operator’s Manual 5. Place an empty filter bag in the Bag Holder in an open position. 6. Tare the weight of the empty filter bag and the holder together. 7. Add 0.95 – 1.00g of sample to the filter bag. Keep all particles away from the sealing area of the filter bag. 8. Record the weight of the sample (W2). 9. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red sealer light turns off (to cool the seal).
Operator’s Manual Crude Fiber Analysis Procedure using the ANKOM200 Fiber Analyzer To perform Crude Fiber analysis on prepared samples, follow the procedure detailed below. The Vessel must be at room temperature before beginning a Crude Fiber analysis. 1. Verify that the exhaust hose is connected to the instrument and securely positioned in the drain. 2. Turn the instrument Power Switch to the ON position. 3. Lift the Lid Handle to open the Vessel Lid. 4.
Operator’s Manual 16. When the Timer sounds, the rinse is complete. Slowly open the Exhaust Valve to drain the hot water. 17. Repeat steps 12 - 16 to accomplish the second rinse. 18. Add 1900 ml - 2000 ml of ambient temperature base (0.313N NaOH) solution. 19. Press the HEAT and AGITATE buttons on the front of the instrument so they are lit. Look in the Vessel to confirm agitation. 20. Close the Vessel Lid. 21. On the Timer/Clock, set the Timer to 40 minutes and press the Start/Stop button. 22.
Operator’s Manual 37. Remove the samples from the oven and place them directly in a Desiccant Pouch. Flatten the pouch to remove ambient air and zip it tight. Do NOT use conventional countertop or cabinet desiccators for this analysis. 38. Allow the samples to cool to room temperature. This should take about 10 - 15 minutes. 39. Remove one filter bag from the Desiccant Pouch. Flatten the pouch to remove ambient air.
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Operator’s Manual Periodic Maintenance Initial Maintenance (during the first 20 hours of operation) Follow the procedure below: 1. After every 3 hours of use check the Maintenance Alert Reservoir for any fluid accumulation. 2. If any fluid is present, unscrew the Reservoir bottle and empty the fluid. 3. Screw the Reservoir into the instrument. Rear View Maintenance Alert Collector 4. Turn the instrument off and disconnect the power cord from the power source. 5.
Operator’s Manual Replacing the Fuse in a 120V instrument To replace the fuse in a 120V ANKOM200 Fiber Analyzer, follow the procedure detailed below. 1. Turn off the instrument power and unplug the Power Cord from the outlet. 2. Using a flat blade screwdriver, twist the slot on the fuse holders clockwise ¼ turn to open. 3. Replace the 15 amp glass or ceramic fuse. 4. Install the fuse in the fuse holder. 5. Re-install the fuse holder into the instrument.
Operator’s Manual Check the Agitation System The agitation system should be checked every three to six months or if fiber values are higher than normal or inconsistent. To check the Agitation System, follow the procedure detailed below. Poor agitation will cause higher analysis values and poor repeatability. 1. 2. Check the function of the agitator motor. 1.1 Place a full bag suspender in the Vessel along with the bag suspender weight, but add NO water. 1.2 Press the AGITATE button so that it is lit.
Operator’s Manual Check the Bag Suspender The Bag Suspender should be checked every three to six months or if fiber values are higher than normal or inconsistent. To check the Bag Suspender, follow the procedure detailed below. Melted trays 1. Check the trays for melting. The pictures shown are examples of extreme cases. However, for proper operation you must replace trays that show signs of melting or wear. 2. Check that the bottom tray is flat.
Operator’s Manual Appendix A – ADF Method Acid Detergent Fiber in Feeds - Filter Bag Technique (for A200 and A200I) Definition This method determines Acid Detergent Fiber, which is the residue remaining after digesting with H 2SO4 and CTAB. The fiber residues are predominantly cellulose and lignin. Scope This method is applicable to grains, feeds, forages, and all fiber-bearing material. Apparatus ADF Procedure (see the ADF Analysis section of the Operator’s 1. 2. Manual for more detail) 3. 4. 5.
Operator’s Manual Calculations ADF Procedure (continued) % ADF (as-received basis) = 100 x (W3 – (W1 x C1)) 8. W2 Where: W1 W2 W3 = = = C1 = Bag tare weight Sample weight Dried weight of bag with fiber after extraction process Blank bag correction (running average of final oven-dried weight divided by original blank bag weight) 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. pg. 32 Place up to 3 bags on each of eight Bag Suspender Trays (maximum of 24 bags).
Operator’s Manual Appendix B – NDF Method Neutral Detergent Fiber in Feeds - Filter Bag Technique (for A200 and A200I) Definition This method determines Neutral Detergent Fiber, which is the residue remaining after digesting in a detergent solution. The fiber residues are predominantly hemicellulose, cellulose, and lignin. Scope This method is applicable to grains, feeds, forages, and all fiber-bearing material. Apparatus NDF Procedure (see the NDF Analysis section of the Operator’s 1. 2.
Operator’s Manual Calculations NDF Procedure (continued) % NDF (as-received basis) = 100 x (W3 – (W1 x C1)) 8. W2 Where: W1 W2 W3 = = = C1 = Bag tare weight Sample weight Dried weight of bag with fiber after extraction process Blank bag correction (running average of final oven-dried weight divided by original blank bag weight) 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. pg. 34 Place up to 3 bags on each of eight Bag Suspender Trays (maximum of 24 bags).
Operator’s Manual Appendix C – Crude Fiber Method (AOCS Ba 6a-05) Crude Fiber Analysis in Feeds - Filter Bag Technique (for A200 and A200I) Definition This method determines Crude Fiber which is the organic residue remaining after digesting with 0.255N H 2SO4 and 0.313N NaOH. The compounds removed are predominantly protein, sugar, starch, lipids and portions of both the structural carbohydrates and lignin.
Operator’s Manual Calculations % Crude Fiber = 100 x (W3 – (W1 x C1)) W2 Where: pg. 36 W1 W2 W3 = = = C1 = Bag tare weight Sample weight Weight of Organic Matter (loss of weight on ignition of bag and fiber) Ash corrected blank bag factor (running average of loss of weight on ignition of blank bag/original blank bag) 11. Open the Vessel Lid and insert the Bag Suspender with bags into the Vessel and place the Bag Suspender Weight on top of the empty 9th tray to keep the Bag Suspender submerged. 12.
Operator’s Manual Table 1. Results of the international collaborative study of the Filter Bag Technique for crude fiber compared with three laboratories using an Official Crude Fiber Method. Collaborative Whole Cattle Whole Poultry Calf Swine Horse Soy Pig Dog Laboratory No. Rep Corn Feed Alfalfa Soy Starter Starter Feed Feed Meal Starter Food 1 2 3 4 5 6 7 8 9 10 11 Mean 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 2.1 1.8 1.7 2.0 1.6 1.9 1.6 1.7 1.5 1.8 1.8 2.0 1.7 1.5 1.6 1.6 1.4 1.8 1.7 1.7 1.4 1.5 1.
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Operator’s Manual Appendix D – Wiring Diagram Rev C 1/14/15 pg.
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