Operator’s Manual Rev E 1/14/15 2052 O’Neil Rd, Macedon NY 14502 Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.
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Table of Contents Introduction .................................................................................................................................................................. 5 Warranty ....................................................................................................................................................................... 5 Filter Bags ............................................................................................................................
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Operator’s Manual Introduction ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical laboratories around the world in the environmental, agricultural, biomass, and food industries. ANKOM Technology can provide you with products for determining or monitoring detergent fibers, dietary fibers, fat, digestibility, microbial fermentation (anaerobic or aerobic) and more.
Operator’s Manual Contact Information At ANKOM Technology we are committed to your total satisfaction and therefore always available to help you get the most from your ANKOM products. We are also very interested in any comments or suggestions you may have to help us improve. For any questions or suggestions regarding your instrument, please contact us at: pg. 6 Telephone: (315) 986-8090 Fax: (315) 986-8091 Email: service@ankom.com www.ankom.
Operator’s Manual Instrument Description General Description The ANKOM2000 Fiber Analyzer is designed to efficiently and accurately determine Acid Detergent Fiber (ADF), Neutral Detergent Fiber (NDF), and Crude Fiber within food and/or feed samples. Enabled by Filter Bag Technology, up to 24 samples can be processed at one time.
Operator’s Manual Port A – Used for Crude Fiber Acid solution and ND solution Vent Tube Water Supply Fitting Water Filter Line to Hot Water Supply Drain Hose Left Side View Port B – Used for Crude Fiber Base solution, AD solution, and Diluted Amylase Temperature Controller Fuses Domestic: 15A/120V International: 10A/220V Power Cord Inlet Power Switch Right Side View pg.
Operator’s Manual Internal Components A2000 Computer (part # A09) Valve B (part # A19 B) Check Valve Assembly Pressure Transducer (part # 6121) & Fitting Assembly (part # A25) Valve A (part # A19 A) SICK Fiber Optic Module - for Instruments delivered before 4/09 (part # A18) Analog Level Sensor Module - for Instruments delivered after 4/09 (part # A31) Level Sensor Fitting 24V DIN Rail Vent Valve (part # A22) ETS Switch Digestion Vessel 24 VDC Power Supply (part # 963) Temperature Probe (part # A
Operator’s Manual Safety Precautions Hazardous Pressure – Do NOT open the Vessel Lid during operation. The contents of the Vessel are hot and under pressure. Failure to observe this caution may result in scalding or burning. Hot Surfaces – Do NOT touch the Vessel surfaces during operation. The surface can exceed 70°C (158°F). Failure to observe this caution may result in burning. Hazardous Voltages – Do NOT operate the instrument with the cover removed. Hazardous voltages are present during operation.
Operator’s Manual Instrument Installation Site Requirements To install and operate the ANKOM2000 Fiber Analyzer you will need the following: Adjustable wrench Water supply located close to the ANKOM2000 capable of heating water to 50°C for Crude Fiber and 70°C for ADF/NDF analyses Adequate power (see “Operating Environment” section) Drain Instrument Installation Procedure To install the ANKOM2000 Fiber Analyzer, follow the procedure detailed below. 1.
Operator’s Manual 6. If you are using Cubetainers for your chemicals, install the Cubetainer Shelf shown below (Optional). IMPORTANT The solutions are gravity fed into the instrument ports. The bottom of the solution level must be at least 5” (13cm) above the port. The top of the solution level can be no more than 20” (50cm) above either port or the solution will overflow into the drain. pg.
Operator’s Manual Fiber Analysis Support Items The following support items are needed to perform the fiber analysis procedures: Item Recommended Product Electronic Balance with four-place readout ANKOM #TB Balance Hardware ANKOM #TBS Balance Software ANKOM #F57 ANKOM #101.
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Operator’s Manual ADF Analysis ADF Calculation ADF contained within a food or feed sample can be calculated using the following formula: % ADF (as-received basis) = 100 x (W3 – (W1 x C1)) W2 Where: = = = = Bag tare weight Sample weight Dried weight of filter bag with fiber after extraction process Blank bag correction (running average of final oven-dried weight divided by original blank bag weight) W1 W2 W3 C1 ADF Sample Preparation Procedure To prepare samples for fiber analysis, follow the procedu
Operator’s Manual 5. Place an empty filter bag in the Bag Holder in an open position. 6. Tare the weight of the empty filter bag and the holder together. 7. Add 0.45 – 0.50g of sample to the filter bag. Keep all particles away from the sealing area of the filter bag. 8. 9. Record the weight of the sample (W2) and tare. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red sealer light turns off (to cool the seal).
Operator’s Manual ADF Analysis Procedure using the ANKOM2000 Fiber Analyzer To perform ADF analysis on prepared samples, follow the procedure detailed below. IMPORTANT If you are changing from one analysis type to another, flush the system before running. See the “Flush Procedure” section of this manual for more information. 1. 2. Verify that the hot water supply is on and the drain hose is securely positioned in the drain.
Operator’s Manual 8. 9. Press ENTER on the Keypad and follow the prompts on the Display to set up the instrument for ADF analysis. Close the Vessel Lid and tighten it by turning the Vessel Clamp Handle. If you do not want to use the Cubetainers to automatically add your solutions, you can manually fill the vessel for each procedure. First make sure that the hoses to the Cubetainers are disconnected. Then press START on the Keypad and immediately pour 2L of solution directly into the vessel.
Operator’s Manual NDF Analysis NDF Calculation NDF contained within a food or feed sample can be calculated using the following formula: % NDF (as-received basis) = 100 x (W3 – (W1 x C1)) W2 Where: = = = = Bag tare weight Sample weight Dried weight of filter bag with fiber after extraction process Blank bag correction (running average of final oven-dried weight divided by original blank bag weight) W1 W2 W3 C1 NDF Sample Preparation Procedure To prepare samples for fiber analysis, follow the procedu
Operator’s Manual 5. Place an empty filter bag in the Bag Holder in an open position. 6. Tare the weight of the empty filter bag and the holder together. 7. Add 0.45 – 0.50g of sample to the filter bag. Keep all particles away from the sealing area of the filter bag. 8. 9. Record the weight of the sample (W2) and tare. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red sealer light turns off (to cool the seal).
Operator’s Manual NDF Analysis Procedure using the ANKOM2000 Fiber Analyzer To perform NDF analysis on prepared samples, follow the procedure detailed below. IMPORTANT If you are changing from one analytical method to another, flush the system before running. See the “Flush Procedure” section of this manual for more information. Amylase tends to be sticky.
Operator’s Manual 11. Press the arrow keys on the Keypad until you see “Select NDF” on the Display. Select NDF v IMPORTANT The NDF analysis will use the following default settings: 75 minute digestion four 5 minute rinses If you want to run a custom NDF analysis that allows you to set the digestion time and the number of rinses, press the arrow keys until you see “Select Custom” on the Display. 12.
Operator’s Manual IMPORTANT Do NOT place bags in the oven until the acetone has completely evaporated. 20. Place air-dried bags in the oven and heat at 102°C ± 2° for 2 – 4 hours (depending on the oven). 21. Remove the samples from the oven and place them in a Desiccant Pouch. IMPORTANT Do NOT use conventional countertop or cabinet desiccators for this analysis. 22. Allow the samples to cool to room temperature. This should take about 10 – 15 minutes. 23. Remove one filter bag from the Desiccant Pouch.
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Operator’s Manual Crude Fiber Analysis Crude Fiber Calculation Crude Fiber contained within a food or feed sample can be calculated using the following formula: % Crude Fiber = 100 x (W3 – (W1 x C1)) W2 Where: = = = = Bag tare weight Sample weight Weight of Organic Matter (loss of weight on ignition of bag and fiber) Ash corrected blank bag factor (running average of loss of weight on ignition of blank bag / original blank bag) W1 W2 W3 C1 Crude Fiber Sample Preparation Procedure To prepare samples
Operator’s Manual 5. Place an empty filter bag in the Bag Holder in an open position. 6. Tare the weight of the empty filter bag and the holder together. 7. Add 0.95 – 1.00g of sample to the filter bag. Keep all particles away from the sealing area of the filter bag. 8. Record the weight of the sample (W2) and tare. 9. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red sealer light turns off (to cool the seal).
Operator’s Manual Crude Fiber Analysis Procedure using the ANKOM2000 Fiber Analyzer To perform Crude Fiber analysis on prepared samples, follow the procedure detailed below. 1. Verify that the hot water supply is on and the drain hose is securely positioned in the drain. 2. Attach each Cubetainer hose first to the Cubetainer and then to its specific chemical port. Port A is used for Crude Fiber Acid solution. Port B is used for Crude Fiber Base solution.
Operator’s Manual IMPORTANT The Crude Fiber analysis will use the following settings: 40 minute Acid Digestion 40 minute Base digestion two 5 minute Acid rinses three 5 minute Base rinses If you want to run a custom Crude Fiber analysis that allows you to set the digestion times and the number of rinse cycles, press the arrow keys until you see “Select Custom” on the Display. 8. Press ENTER on the Keypad and follow the prompts on the Display to set up the instrument for Crude Fiber analysis. 9.
Operator’s Manual 16. Remove the samples from the oven and place them in a Desiccant Pouch. IMPORTANT Do NOT use conventional countertop or cabinet desiccators for this part of the analysis. 17. Allow the samples to cool to room temperature. This should take about 10 – 15 minutes. 18. Re-weigh each filter bag immediately after removing from the Desiccant Pouch. 19. Ash all filter bags in pre-weighed crucibles for 2 hours at 600°C ± 15°. 20. Cool the ashed crucibles in a conventional desiccator. 21.
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Operator’s Manual Flush Procedure The Flush procedure allows you to clean the system with water. This procedure should be used when changing from one procedure to another (e.g., when preparing to do an ADF analysis after completing an NDF analysis) or when doing an NDF procedure after a previous NDF procedure with more than two hours between procedures, or before storing the instrument. IMPORTANT Amylase tends to be sticky.
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Operator’s Manual Periodic Maintenance Initial Maintenance (after 10 hours of operation) After the first 10 hours of operation, follow the procedure below: Packing Nut 1. Remove the back panel of the instrument. 2. Inspect the Maintenance Alert Collector. 3. Clean any residue from the collector. 4. Unscrew the filter body (the fixture on the outside of the instrument that attaches to the water supply). 5. Unscrew and clean the metal filter screen. 6.
Operator’s Manual If you see a leak If you see a leak, follow steps 6 – 9 in the Initial Maintenance procedure above. Replacing the Fuses To replace the fuses in the ANKOM2000 Fiber Analyzer, follow the procedure detailed below. 1. Turn off the instrument power and unplug the Power Cord from the outlet. 2. Using a flat blade screwdriver, twist the slot on the fuse holders counterclockwise ¼ turn to open. 3. Check both fuses. 4. Pull the fuse from the grey fuse cap and replace as needed.
Operator’s Manual Check Agitation System and Bag Suspender The agitation system and bag suspender should be checked every three to six months or if fiber values are higher than normal or inconsistent. IMPORTANT Poor agitation will cause higher analysis values and poor repeatability. To check the stroke of the agitation system, follow the procedure detailed below. 1. 2. 3. 4. 5. 6. 7. 8. Place a full bag suspender in the Vessel (without the bag suspender weight), but add NO water.
Operator’s Manual Check Bag Suspender The bag suspender should be checked every three to six months or if fiber values are higher than normal or inconsistent. Check the trays for melting. The pictures shown are examples of extreme cases. However, for proper operation you must replace trays that show signs of melting or wear. Melted trays Check that the bottom tray is flat. If the bottom tray is concave (see picture) the bag suspender will catch in the vessel and melt.
Operator’s Manual Appendix A – ADF Method Acid Detergent Fiber in Feeds - Filter Bag Technique (for A2000 and A2000I) Definition This method determines Acid Detergent Fiber, which is the residue remaining after digesting with H2SO4 and CTAB. The fiber residues are predominantly cellulose and lignin. Scope This method is applicable to grains, feeds, forages, and all fiber-bearing material. Apparatus ADF Procedure (see the ADF Analysis section of the Operator’s 1. 2. Manual for more detail) 3. 4. 5.
Operator’s Manual Calculations ADF Procedure (continued) % ADF (as-received basis) = 100 x (W3 – (W1 x C1)) W2 Where: W1 W2 W3 = = = C1 = Bag tare weight Sample weight Dried weight of bag with fiber after extraction process Blank bag correction (running average of final oven-dried weight divided by original blank bag weight) Numbered Notes 1. A running average blank bag correction factor (C1) should be used in the calculation of fiber.
Operator’s Manual Appendix B – NDF Method Neutral Detergent Fiber in Feeds - Filter Bag Technique (for A2000 and A2000I) Definition This method determines Neutral Detergent Fiber, which is the residue remaining after digesting in a detergent solution. The fiber residues are predominantly hemicellulose, cellulose, and lignin. Scope This method is applicable to grains, feeds, forages, and all fiber-bearing material. Apparatus NDF Procedure (see the NDF Analysis section of the Operator’s 1. 2.
Operator’s Manual Calculations NDF Procedure (continued) % NDF (as-received basis) = 100 x (W3 – (W1 x C1)) W2 Where: W1 W2 W3 = = = C1 = Bag tare weight Sample weight Dried weight of bag with fiber after extraction process Blank bag correction (running average of final oven-dried weight divided by original blank bag weight) Numbered Notes 1. A running average blank bag correction factor (C1) should be used in the calculation of fiber.
Operator’s Manual Appendix C – Crude Fiber Method Crude Fiber Analysis in Feeds - Filter Bag Technique (for A2000 and A2000I) AOCS Approved Procedure Ba 6a-05 Definition This method determines Crude Fiber which is the organic residue remaining after digesting with 0.255N H 2SO4 and 0.313N NaOH. The compounds removed are predominantly protein, sugar, starch, lipids and portions of both the structural carbohydrates and lignin.
Operator’s Manual Precision Crude Fiber Procedure (continued) Results of the collaborative study (see Tables 1&2) indicate the precision (Sr, RSDr, r) that the analyst should use as a benchmark for evaluating replication in the same laboratory. 10. Attach each Cubetainer hose to the Cubetainer and to its specific port. Port A is used for Crude Fiber Acid solution. Port B is used for Crude Fiber Base solution. 11.
Operator’s Manual Table 1. Results of the international collaborative study of the Filter Bag Technique for crude fiber compared with three laboratories using an Official Crude Fiber Method. Collaborative Whole Cattle Whole Poultry Calf Swine Horse Soy Pig Dog Laboratory No. Rep Corn Feed Alfalfa Soy Starter Starter Feed Feed Meal Starter Food 1 2 3 4 5 6 7 8 9 10 11 Mean 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 2.1 1.8 1.7 2.0 1.6 1.9 1.6 1.7 1.5 1.8 1.8 2.0 1.7 1.5 1.6 1.6 1.4 1.8 1.7 1.7 1.4 1.5 1.
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Operator’s Manual Appendix D – Parts & Assemblies 101.2 Bag Holder X45 Desiccant Pouch 693 Bag Suspender Tray (Bottom) 694 Bag Suspender Tray (Top) F11 Bag Suspender Assembly (with weight) 639.1 Bag Suspender Weight F11.5 Kynar Tip w/ Washer Assembly for Bag Suspender F12.
Operator’s Manual A07 Water Filter Assembly A21 Water Valve Assembly A22 Vent Valve Assembly A19 B or A Valve Assembly (must specify B or A) 414 24V Burkert Valve 1/8” Orifice 6121 Pressure Sensor A25 Pressure Fitting Upgrade Assembly Z132 Valve Seats A02 Water Heater Assembly 120v A30 Water Heater Assembly 220v 6032 Solid State Relay 6114 Solid State Relay 460 Relay 120v 461 Relay 220v 462 Relay 24V 6033 Relay Gold 24V A09 Splat Controller Assembly X90 Splat Display A11 Controller Assemb
Operator’s Manual 963 Power Supply 5653 On/Off Switch 687 Fuse 15A 6035 Fuse 10A 6151 SICK Fiber Optic Level Sensor Module (for instruments delivered before 4/09) A18 SICK Optic Cable Assembly (for instruments delivered before 4/09) 6152 Fiber Optic Glass Tip 1/5” A17 Temperature Probe Assembly A31 Analog Optic Cable Assembly (for instruments delivered after 4/09) 6158 Analog Level Sensor Module (for instruments delivered after 4/09) A08 Motor Assembly 120v A10 Motor Assembly 220v F8.
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