Operator’s Manual Rev L 11/12/14 2052 O’Neil Rd, Macedon NY 14502 Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.
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Table of Contents Introduction .................................................................................................................................................................. 5 Warranty ....................................................................................................................................................................... 5 Filter Bags ............................................................................................................................
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Operator’s Manual Introduction ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical laboratories around the world in the food, feed, bio-energy, agricultural, and environmental industries. ANKOM Technology can provide you with products for determining or monitoring dietary fiber, detergent fiber, crude fiber, fat, digestibility, microbial fermentation (anaerobic or aerobic) and more.
Operator’s Manual Contact Information ANKOM Technology is committed to your total satisfaction and therefore always available to help you get the most from your ANKOM products. We are also very interested in any comments or suggestions you may have to help us improve. For any questions or suggestions regarding your instrument, please contact us at: pg. 6 Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.
Operator’s Manual Instrument Description General Description The ANKOMHCl Hydrolysis System is designed to release complexed lipids from multiple food or feed samples at the same time in an automated fashion using acid hydrolysis so that the fat from the samples can then be extracted using fat solvents. Fat extraction can be done using the ANKOMXT15 and ANKOMXT10 Extractors. Below is a detailed exterior view of the ANKOMHCl Hydrolysis System.
Operator’s Manual Safety Precautions Hot Surfaces – Do not touch the Vessel Lid during operation. The surface can exceed 70°C (158°F). Failure to observe this caution may result in injury. Hazardous Voltages – Do not operate the instrument with the Top Cover (electrical compartment) open. Hazardous voltages are present during operation. Failure to observe this caution may result in electrical shock or electrocution. Hazardous Materials – Acid solution is corrosive.
Operator’s Manual Instrument Installation and Setup Site Requirements To install and operate the ANKOMHCl Hydrolysis System you will need the following: Adjustable wrench Tap water connection Adequate power (see “Operating Environment” section) Drying capability It is extremely important that you completely dry your food or feed samples in an ANKOMRD Dryer or an oven resistant to HCl fumes.
Operator’s Manual 4. Attach the HCl Zinc Filter Assembly (part# H36) to the “Vent Hose” as shown below. Position the hose and the filter to run downhill from the instrument to prevent condensation from accumulating inside. Vent Hose HCl Zinc Filter Assembly (H36) Water Drain Hose Replace the filter every four months or when pH paper placed at the end of the filter indicates that acid fumes are exiting the filter. 5. Plug the power cord into a grounded receptacle. pg.
Operator’s Manual Instrument Setup This step is extremely important to fill the waste tank in the instrument with water to dilute the waste acid from the first and subsequent runs. To get your ANKOMHCl Hydrolysis System set up and ready for use, follow the procedure detailed below. Display 1. Turn the instrument power switch ON. When the power is on, the switch light and the power light on the Keypad are lit. 2. Turn the water supply on. 3. Pull up on the Lid Latch to open the Hydrolysis Vessel.
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Operator’s Manual Hydrolysis Procedure Total Fat Calculation The hydrolysis procedure is done prior to fat extraction.
Operator’s Manual Sample Categorization For best results, it is important to determine the correct ratio of Diatomaceous Earth (DE) to sample. Before using your ANKOMHCl Hydrolysis System for production testing, it is recommended that you categorize your samples into types and determine the standard amount of DE needed for each sample type. The following information is from testing done in the ANKOM lab. This information is meant to help you categorize your own samples.
Operator’s Manual Sample Categorization Data from ANKOM Lab (sorted by DE Weight) Sample Type Fat % DE Weight Sample Weight Notes Whole Milk ~ 3% 1.25 g 1.25 g Whipping Cream 35% 1.25 g 0.5 g After adding the DE to the filter bag, press the eraser end of a pencil into the DE to create an indentation into which the milk can be added using a pipette. Record the weight (to make sure DE was not transferred to the pencil) & tare.
Operator’s Manual Sample Preparation Procedure To avoid fat migration from one filter bag to another during the hydrolysis procedure, it is very important to prepare your samples properly. Before running production samples, it is recommended that you do the following: Categorize your samples to determine the standard amount of DE needed for each of your specific sample types. See the “Sample Categorization” section for details. Mix dry/granular samples with the correct amount of DE.
Operator’s Manual Moist Samples a. Add 1/2 of the recommended amount of DE to the filter bag. b. Using a spatula, make a valley or a space in the DE for the sample. c. Record the weight and tare. d. Place an appropriate amount of sample into the DE valley in the filter bag, being careful that the sample does not touch the inside walls of the filter bag so the sample can be fully covered with DE. e. Record the weight of the sample (W1) and tare. f.
Operator’s Manual 11. Include two blank bags (C1 and C2) filled with approximately 0.5g – 0.75g of DE. Blank bags serve as correction factors for slight losses of weight that may occur during extraction. Blank bags with DE also act as indicators of fat loss during hydrolysis. When placed next to a sample that loses fat, a blank bag will absorb some of the fat lost by the other bag. The result is a blank loss during extraction that exceeds the normal expected blank loss of 0.0025g by 0.0005g or more.
Operator’s Manual Hydrolysis step-by-step procedure using the ANKOMHCl Hydrolysis System To perform hydrolysis on prepared samples, follow the procedure detailed below. Display Keypad 1. Turn the instrument power switch ON. Lid Latch 2. Turn the cold water supply on. 3. Pull the Lid Latch up to open the Vessel Lid. 4. Place the Multi-bag Holder into the Hydrolysis Vessel. 5. Pour 500ml of 3N HCl into the Hydrolysis Vessel to cover the ports in the back. 6.
Operator’s Manual 16. Press START on the Keypad. The ANKOMHCl Hydrolysis System will automatically heat and maintain the set temperature. After hydrolysis is complete, the samples are automatically rinsed with cold water. When the instrument has completed the automated process, the Display will show “Process Complete”. 17. When the automated process is complete, open the Lid Latch and immediately wipe the Teflon pad on the underside of the lid with paper towel.
Operator’s Manual Periodic Maintenance Cleaning the exterior of the instrument At the end of each day use an ammonia-based cleaner (a mild base solution) and cloth to wipe the outside of the instrument, making sure to clean the hinge on the Vessel Lid. Do NOT use Acetone to clean the instrument. Cleaning the interior of the Vessel To restore the white color to the Vessel, periodically run a bleach solution through the instrument using the procedure detailed below. 1. Turn the water supply on. 2.
Operator’s Manual Fuse Holders 5. Press the tab and lift the Fuse Holder up. Tab to open Fuse Holders Fuse 6. Replace the fuses and close the Fuse Holder completely. 7. Close the Top Cover and secure it with the two screws that you previously removed. Cleaning/Checking the Water Filter To clean/check the Water Filter in the ANKOMHCl Hydrolysis System, follow the procedure detailed below. 1. Unscrew the Water Supply Connection and barb fitting from the HCl instrument. Note the arrow orientation 2.
Operator’s Manual Troubleshooting & Replacement Parts The ANKOM Technology web site has the most current troubleshooting and replacement parts information. If you have any questions about the operation of your ANKOMHCl Hydrolysis System, or if you need replacement parts, please visit www.ankom.com. Rev L 11/12/14 pg.
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Operator’s Manual Appendix A – Electrical Diagram (pg. 1 of 3) Rev L 11/12/14 pg.
Operator’s Manual Appendix A – Electrical Diagram (pg. 2 of 3) pg.
Operator’s Manual Appendix A – Electrical Diagram (pg. 3 of 3) Rev L 11/12/14 pg.
Automation saves time and money! ANKOM Technology is an international company with products that include… TDF Dietary Fiber Analyzer Automates AOAC 991.43 and AACC 32-07.01 Automates AOAC 2009.01/2011.25 and AACC 32-45.
