Operator’s Manual Rev E 12/2/14 2052 O’Neil Rd, Macedon NY 14502 Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.
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Table of Contents Introduction .................................................................................................................................................................. 5 Warranty ....................................................................................................................................................................... 5 Filter Bags .............................................................................................................................
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Operator’s Manual Introduction ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical laboratories around the world in the food, feed, bio-energy, agricultural, and environmental industries. ANKOM Technology can provide you with products for determining or monitoring dietary fiber, detergent fiber, crude fiber, fat, digestibility, microbial fermentation (anaerobic or aerobic) and more.
Operator’s Manual Method Comparison (Manual vs. Automated) AOAC 991.43 Method – IDF/SDF assay without automation Below is the AOAC 991.43 method flow done manually by a technician using glass crucibles and beakers, vacuum to assist with filtering, and water baths to control temperature and mixing. DE refers to Diatomaceous Earth.
Operator’s Manual Instrument Description The ANKOMTDF Dietary Fiber Analyzer is designed to efficiently, accurately, and precisely recover Insoluble Dietary Fiber (IDF), Soluble Dietary Fiber (SDF), and Total Dietary Fiber (TDF) residue from food and/or feed samples in accordance with the AOAC 985.29, AOAC 991.43, AACC 32-07.01, and NMKL 129, 2003 methods. Contact ANKOM Technology about an upgrade to Total Integrated Dietary Fiber (AOAC 2009.01 and 2011.25 methods).
Operator’s Manual External Components – Left Side View Power Switch Front Cover Arm Front Cover (TDF56) Enzyme Containers (TDF35, TDF36, TDF37) Nitrogen Supply Port 100 psi (6.9 bar) HCl Container (TDF38) Single Container Holder External Components – Right Side View Front Cover Arm 78% EtOH Container (TDF41) Control Panel (TDF17) Buffer Container (TDF39) Distilled Water Container (TDF40) 95% EtOH Container (TDF42) Three Container Holder pg.
Operator’s Manual Internal Components – Rear View In-Line Heater Main Electrical Cabinet IDF Inlet Valve Stations 4, 5 & 6 Tubing Support Panel IDF Inlet Valve Stations 1, 2 & 3 SDF Inlet Valve Stations 1, 2 & 3 SDF Inlet Valve Stations 4, 5 & 6 Torsion Bar N2 Check Valve (1 of 12) Pressure Sensors Vacuum Sensor Waste Valves (1 – 6) Supply Valves Water Buffer 78% EtOH 95% EtOH Peristaltic Pump Supply Manifold Clamp Bar Cylinder (1 of 8) Supply Valves Amylase Protease AMG HCl Supp
Operator’s Manual Service Access Position If your instrument must be located in such a way as to restrict access to the back, you can tilt it forward to access the rear of the instrument by removing the two Service Access Screws on the front of the instrument and gently pulling the top of the Main Electrical Cabinet forward and down. The instrument will pivot at its center.
Operator’s Manual Instrument Installation Site Requirements To install and operate the ANKOMTDF Dietary Fiber Analyzer you will need the following: Adequate power (see “Operating Environment” section of this manual) Drain or waste container to capture waste fluids 100 psi (6.9 bar) Nitrogen Supply and connection tubing Because the instrument is powered by pneumatics, do NOT run it without an adequate Nitrogen Supply.
Operator’s Manual 1 – Pinch Valve Tubing Set (TDF71) 1 – Pump Tube Set (TDF68) 1 - Line Flush Tubing assy (TDF70) 1 – Amylase Container assy (TDF35) 1 – Protease Container assy (TDF36) 1 – HCl Container assy (TDF38) 1 – Mes-Tris Buffer Container assy (TDF39) 1 – Distilled Water Container assy (TDF40) 1 – 78% EtOH Container assy (TDF41) 1 – 95% EtOH Container assy (TDF42) 6 – Filtrate Cups (TDF53) 1 – Control Panel assy (TDF17) 1 – Bag Weigh Holder assy (TDF52) 1 – Drying Rack assy (TDF50) 2 –
Operator’s Manual 3. 1 – Amylase/AMG Container assy (TDF57) 1 – Trizma Container assy (TDF58) (ONLY with AOAC 2009.01/2011.25 methods) (ONLY with AOAC 2009.01/2011.25 methods) 1 - Enzyme Pinch Bar (8167) 1 - Spray Tip Cleaning Tool (8130) 1 – Maleate Buffer Container assy (TDF39) & Fluid Tube Label (ONLY with AOAC 2009.01/2011.25 methods) Place the Waste Tray under the front of the instrument.
Operator’s Manual 7. Connect the Control Panel to the instrument. Remove the nuts from the right side of the instrument. Connect the 25 pin connector from the instrument to the Control Panel. Slide the Control Panel onto the bolts and secure it in place using the nuts. Bolts for Control Panel 25 pin connector 8. Place the Chemical Containers in their holders.
Operator’s Manual 10. Connect the Enzyme Containers to the corresponding ports. The Enzyme Containers connect into labeled, color-coded ports on the left side of the instrument using push-on connectors. Make sure that you hear a click sound when you connect the enzyme containers to the ports. Push-on Connector 11. Connect the Nitrogen Supply to the Nitrogen Supply Port. The Nitrogen Supply Port has a Push-to-Connect fitting that accepts ¼” OD tubing.
Operator’s Manual Dietary Fiber Analysis Support Items The following support items needed to perform the fiber analysis may be purchased separately: Item ANKOM Part # Electronic Balance with four-place readout Electronic Balance Software Heat Sealer for sealing the filter bags IDF Filter Bags SDF Filter Bags (used for SDF and TDF procedures) IDF Flow-Thru Bags (TDF procedure only) Solvent Resistant Marker Rinse Stand Diatomaceous Earth (DE) Drying Oven TB TBS 1915 (120V), 1920 (220V) DF-I DF-S DF-FT F0
Operator’s Manual IDF/SDF Analysis An IDF/SDF analysis measures the amount of IDF and SDF within a given sample. This requires the use of ANKOM DF-I and DF-S filter bags. When starting a new run, the ANKOMTDF Dietary Fiber Analyzer must have bags installed at all stations (a total of twelve bags). To enhance the productivity of your instrument, you can begin the IDF process of a new run while the SDF process of a previous run is finishing.
Operator’s Manual 6. Weigh Diatomaceous Earth (DE). DE is used during fiber analysis to enhance the flocculation and filtration of the SDF fraction. Place ca 1 g of DE in each of six tared and numbered tins and record the weights. 7. Weigh Samples. Place 0.50.05 g of sample in each of six tared and numbered tins and record the weights. 8. Turn the instrument power on. When you turn the power on, the instrument will run through an initialization process and the Control Panel will turn on. 9.
Operator’s Manual Line Charge ← Amylase Protease AMG HCl EtOH78 EtOH95 Water Buffer ALL 9.1.3 Press the ALL button to charge all of the lines. Each button will change color as the associated line is being charged. All lines are charged when all of the buttons return to their original green color. 9.1.4 Press the back button twice. The following “Select a Function” screen will be displayed again. Select a Function AOAC 991.43 IDF/SDF AOAC 985.29 TDF AOAC 991.43 TDF Diagnostics 9.
Operator’s Manual 10. Install SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C, and D by lifting them off of the locator rods. Clamp Bar A Clamp Bar B Clamp Bar C Clamp Bar D 10.2 Gently pull the black SDF Delivery Nozzle out toward you. 10.3 Place a labeled and weighed SDF bag up underneath the SDF Delivery Nozzle so that the Delivery Nozzle is inside the top part of the bag. Pull the bag up so that the top of the bag is about 35 mm (1.
Operator’s Manual 10.4 With the bag being held by the Delivery Nozzle, center it horizontally between the lines on the back part of Clamp Bar C. Centering Lines 10.5 10.6 10.7 10.8 Repeat steps 10.2 – 10.4 for all six stations. Re-install Clamp Bar D by setting it on the locator rods. Make sure the letter is on the top of the bar and the rubber material is facing in toward the instrument. Flatten the bags to remove any wrinkles.
Operator’s Manual 11.4 Press the check mark () button on the Touch Screen Display. The following screen will be displayed. AOAC 991.43 IDF/SDF ← IDF Bags (and clamp bar B) installed? After you confirm that the DE is added, the Clamp Bar D button on the Control Panel is disabled until the SDF process completes. 12. Install IDF filter bags on the ANKOMTDF Dietary Fiber Analyzer. 12.1 Gently pull the black IDF Delivery Nozzle out toward you. 12.
Operator’s Manual 12.4 Place at least 20 mm (0.75 inches) of the filter section of the IDF bag inside the top of the SDF bag to allow for the flow of liquid into the SDF bag after the IDF process is complete. Heating Paddle Top part of the filter is positioned just below the bottom of Clamp Bar B 12.5 12.6 12.7 12.8 Clamp Bar B 20 mm (0.75 inches) of the IDF filter inside the top of the SDF bag Repeat steps 12.1 – 12.4 for all six stations. Re-install Clamp Bar B by setting it on the locator rods.
Operator’s Manual 13.2 13.3 13.4 13.5 Rinse the tin with no more than 3 ml of Distilled Water to ensure complete transfer. Repeat steps 13.1 – 13.2 for all IDF stations. Re-install Clamp Bar A by setting it on the locator rods. Make sure the letter is on the top of the bar and the rubber material is facing in toward the instrument. Press the check mark () button on the Touch Screen Display. The following screen will be displayed. AOAC 991.43 IDF/SDF ← All remaining clamp bars installed? 13.
Operator’s Manual ← AOAC 991.43 IDF/SDF Check supply containers Amylase -> Amylase AMG -> AMG HCL -> HCL Buffer -> Mes Tris Buffer 15. Verify that the fluid containers have the correct fluids for this procedure. Verify that the fluid supply containers are configured according to the screen above and press the check mark () button. The following screen will be displayed. AOAC 991.43 IDF/SDF ← Fluid levels correct? 16. Fill fluid containers.
Operator’s Manual 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in your lab is connected to the instrument and turned on. The pressure gauges on the instrument should show 50-55 psi on the left and about 4 psi on the right. Press the check mark () button on the Touch Screen Display. The following screen will be displayed. AOAC 991.43 IDF/SDF ← Filter minutes OK? (Press button with number to change) IDF 5.0 After release 1.
Operator’s Manual AOAC 991.43 IDF/SDF ← Press START to begin START 20. Start the instrument. The instrument is now completely set up and ready to automatically run the IDF/SDF procedure. Press the START button to begin. At the beginning of each new run, the instrument automatically runs a tube integrity test. For the first run after a power-up cycle, or for the first run after the instrument has been idle for twelve hours, the following screen will be displayed.
Operator’s Manual 21. Manually measure pH. One of the questions you are asked before starting the automated procedure is: “Check pH manually?” If you answered “Yes” to this question, the instrument will stop after adding the required HCl, open Clamp Bar A, display the screen below, and make a sound to remind you to manually measure pH and adjust if necessary. pH required? Status YES Overall Progress IDF SDF AOAC 991.
Operator’s Manual 22.6 With your Heat Sealer set between 3 and 4 (settings may vary depending on the heat sealer and the power source), press the Heat Sealer arm down for 3 to 4 seconds to seal each bag just above the filter. This keeps all residue contained to the filter area while handling the bags. 22.7 Place each bag in the Drying Rack. 23. Rinse the SDF filter bags with acetone.
Operator’s Manual 27. Determine the Protein content within the IDF and SDF residue. See the “Protein Determination” section of this manual for recommended procedures. 28. Determine the Ash content within the IDF and SDF residue. See the “Ash Determination” section of this manual for recommended procedures. 29. Calculate the % IDF value.
Operator’s Manual TDF Analysis (AOAC 991.43) A TDF analysis directly measures the amount of TDF within a given sample without separately measuring the IDF and SDF fractions. TDF can be determined using multiple methods. This section describes the procedure for using the ANKOM TDF Dietary Fiber Analyzer to determine TDF based on the AOAC 991.43 method.
Operator’s Manual 6. Weigh Diatomaceous Earth (DE). DE is used during fiber analysis to enhance the flocculation and filtration of the SDF fraction. Place ca 1 g of DE in each of six tared and numbered tins and record the weights. 7. Weigh Samples. Place 0.50.05 g of sample in each of six tared and numbered tins and record the weights. 8. Turn the instrument power on. When you turn the power on, the instrument will run through an initialization process and the Control Panel will turn on. 9.
Operator’s Manual Line Charge ← Amylase Protease AMG HCl EtOH78 EtOH95 Water Buffer ALL 9.1.3 Press the ALL button to charge all of the lines. Each button will change color as the associated line is being charged. All lines are charged when all of the buttons return to their original green color. 9.1.4 Press the back button twice. The following “Select a Function” screen will be displayed again. Select a Function AOAC 991.43 IDF/SDF AOAC 985.29 TDF AOAC 991.43 TDF Diagnostics 9.
Operator’s Manual 10. Install SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C, and D by lifting them off of the locator rods. Clamp Bar A Clamp Bar B Clamp Bar C Clamp Bar D 10.2 Gently pull the black SDF Delivery Nozzle out toward you. 10.3 Place a labeled and weighed SDF bag up underneath the SDF Delivery Nozzle so that the Delivery Nozzle is inside the top part of the bag. Pull the bag up so that the top of the bag is about 35 mm (1.
Operator’s Manual 10.4 With the bag being held by the Delivery Nozzle, center it horizontally between the lines on the back part of Clamp Bar C. Centering Lines 10.5 10.6 10.7 10.8 Repeat steps 10.2 – 10.4 for all six stations. Re-install Clamp Bar D by setting it on the locator rods. Make sure the letter is on the top of the bar and the rubber material is facing in toward the instrument. Flatten the bags to remove any wrinkles.
Operator’s Manual 11.4 Press the check mark () button on the Touch Screen Display. The following screen will be displayed. AOAC 991.43 TDF ← IDF Flow-Thru Bags (and clamp bar B) installed? After you confirm that the DE is added, the Clamp Bar D button on the Control Panel is disabled until the SDF process completes. 12. Install IDF Flow-thru bags on the ANKOMTDF Dietary Fiber Analyzer. 12.1 Gently pull the black IDF Delivery Nozzle out toward you. 12.
Operator’s Manual 12.4 Place at least 20 mm (0.75 inches) of the bottom of the IDF Flow-Thru bag inside the top of the SDF bag to allow for the flow of solution into the SDF bag after the digestion process is complete. Heating Paddle 20 mm (0.75 inches) of the IDF Flow-Thru bag inside the top of the SDF bag 12.5 12.6 12.7 12.8 Repeat steps 12.1 – 12.4 for all six stations. Re-install Clamp Bar B by setting it on the locator rods.
Operator’s Manual 13.3 13.4 13.5 Repeat steps 13.1 – 13.2 for all IDF stations. Re-install Clamp Bar A by setting it on the locator rods. Make sure the letter is on the top of the bar and the rubber material is facing in toward the instrument. Press the check mark () button on the Touch Screen Display. The following screen will be displayed. AOAC 991.43 TDF ← All remaining clamp bars installed? 13.
Operator’s Manual ← AOAC 991.43 TDF Check supply containers Amylase -> Amylase AMG -> AMG HCL -> HCL Buffer -> Mes Tris Buffer 15. Verify that the fluid containers have the correct fluids for this procedure. Verify that the fluid supply containers are configured according to the screen above and press the check mark () button. The following screen will be displayed. AOAC 991.43 TDF ← Fluid levels correct? 16. Fill fluid containers.
Operator’s Manual 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in your lab is connected to the instrument and turned on. The pressure gauges on the instrument should show 50-55 psi on the left and about 4 psi on the right. Press the check mark () button on the Touch Screen Display. The following screen will be displayed. AOAC 991.43 TDF ← SDF Filter minutes OK? (Press button with number to change) After release 10.0 After EtOH rinses 3.0 18.
Operator’s Manual AOAC 991.43 TDF ← Press START to begin START 20. Start the instrument. The instrument is now completely set up and ready to automatically run the TDF procedure. Press the START button to begin. At the beginning of each new run, the instrument automatically runs a tube integrity test. For the first run after a power-up cycle, or for the first run after the instrument has been idle for twelve hours, the following screen will be displayed.
Operator’s Manual 21. Manually measure pH. One of the questions you are asked before starting the automated procedure is: “Check pH manually?” If you answered “Yes” to this question, the instrument will stop after adding the required HCl, open Clamp Bar A, display the screen below, and make a sound to remind you to manually measure pH and adjust if necessary. pH required? Status YES Overall Progress IDF SDF AOAC 991.
Operator’s Manual 23.6 With your Heat Sealer set between 3 and 4 (settings may vary depending on the heat sealer and the power source), seal each bag just above the filter to keep all residue contained to the filter area while handling the bags. 23.7 Place each bag in the Drying Rack. 24. Dry the SDF filter bags. 24.1 24.2 24.3 Make sure your oven is 105°C at the location where the bags will be placed.
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Operator’s Manual TDF Analysis (AOAC 985.29) A TDF analysis directly measures the amount of TDF within a given sample without separately measuring the IDF and SDF fractions. TDF can be determined using multiple methods. This section describes the procedure for using the ANKOMTDF Dietary Fiber Analyzer to determine TDF based on the AOAC 985.29 method.
Operator’s Manual 6. Weigh Diatomaceous Earth (DE). DE is used during fiber analysis to enhance the flocculation and filtration of the SDF fraction. Place ca 1 g of DE in each of six tared and numbered tins and record the weights. 7. Weigh Samples. Place 0.50.05 g of sample in each of six tared and numbered tins and record the weights. 8. Turn the instrument power on. When you turn the power on, the instrument will run through an initialization process and the Control Panel will turn on. 9.
Operator’s Manual Line Charge ← Amylase Protease AMG HCl EtOH78 EtOH95 Water Buffer ALL 9.1.3 Press the ALL button to charge all of the lines. Each button will change color as the associated line is being charged. All lines are charged when all of the buttons return to their original green color. 9.1.4 Press the back button twice. The following “Select a Function” screen will be displayed again. Select a Function AOAC 991.43 IDF/SDF AOAC 985.29 TDF AOAC 991.43 TDF Diagnostics 9.
Operator’s Manual 10. Install SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C, and D by lifting them off of the locator rods. Clamp Bar A Clamp Bar B Clamp Bar C Clamp Bar D 10.2 Gently pull the black SDF Delivery Nozzle out toward you. 10.3 Place a labeled and weighed SDF bag up underneath the SDF Delivery Nozzle so that the Delivery Nozzle is inside the top part of the bag. Pull the bag up so that the top of the bag is about 35 mm (1.
Operator’s Manual 10.4 With the bag being held by the Delivery Nozzle, center it horizontally between the lines on the back part of Clamp Bar C. Centering Lines 10.5 10.6 10.7 10.8 Repeat steps 10.2 – 10.4 for all six stations. Re-install Clamp Bar D by setting it on the locator rods. Make sure the letter is on the top of the bar and the rubber material is facing in toward the instrument. Flatten the bags to remove any wrinkles.
Operator’s Manual 11.4 Press the check mark () button on the Touch Screen Display. The following screen will be displayed. AOAC 985.29 TDF ← IDF Flow-Thru Bags (and clamp bar B) installed? After you confirm that the DE is added, the Clamp Bar D button on the Control Panel is disabled until the SDF process completes. 12. Install IDF Flow-thru bags on the ANKOMTDF Dietary Fiber Analyzer. 12.1 Gently pull the black IDF Delivery Nozzle out toward you. 12.
Operator’s Manual 12.4 Place at least 20 mm (0.75 inches) of the bottom of the IDF Flow-Thru bag inside the top of the SDF bag to allow for the flow of solution into the SDF bag after the digestion process is complete. Heating Paddle 20 mm (0.75 inches) of the IDF Flow-Thru bag inside the top of the SDF bag 12.5 12.6 12.7 12.8 Repeat steps 12.1 – 12.4 for all six stations. Re-install Clamp Bar B by setting it on the locator rods.
Operator’s Manual 13.3 13.4 13.5 Repeat steps 13.1 – 13.2 for all IDF stations. Re-install Clamp Bar A by setting it on the locator rods. Make sure the letter is on the top of the bar and the rubber material is facing in toward the instrument. Press the check mark () button on the Touch Screen Display. The following screen will be displayed. AOAC 985.29 TDF ← All remaining clamp bars installed? 13.
Operator’s Manual ← AOAC 985.29 TDF Check supply containers Amylase -> Amylase AMG -> AMG HCL -> HCL Buffer -> Phosphate Buffer 15. Verify that the fluid containers have the correct fluids for this procedure. Verify that the fluid supply containers are configured according to the screen above and press the check mark () button. The following screen will be displayed. AOAC 985.29 TDF ← Fluid levels correct? 16. Fill fluid containers.
Operator’s Manual 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in your lab is connected to the instrument and turned on. The pressure gauges on the instrument should show 50-55 psi on the left and about 4 psi on the right. Press the check mark () button on the Touch Screen Display. The following screen will be displayed. AOAC 985.29 TDF ← SDF Filter minutes OK? (Press button with number to change) After release 10.0 After EtOH rinses 3.0 18.
Operator’s Manual AOAC 985.29 TDF ← Press START to begin START 20. Start the instrument. The instrument is now completely set up and ready to automatically run the TDF procedure. Press the START button to begin. At the beginning of each new run, the instrument automatically runs a tube integrity test. For the first run after a power-up cycle, or for the first run after the instrument has been idle for twelve hours, the following screen will be displayed.
Operator’s Manual 21. Adjust pH before the Protease digestion. Just before the Protease digestion (after the bags have cooled to the appropriate temperature) the instrument will stop, open Clamp Bar A, display the screen below, and make a sound to remind you to manually adjust pH. AOAC 985.29 pH adjustment Adjust pH to 7.5 +/- 0.2 by adding NaOH solution. Press "Continue" when complete.
Operator’s Manual 24. Rinse the SDF filter bags with acetone. After the instrument has completed a TDF process the TDF residue that has been collected in the SDF bag must be manually rinsed with acetone. It is recommended that you use an ANKOM TDF51 Rinse Stand (sold separately) for rinsing filter bags with acetone. To rinse the SDF filter bags with acetone using the ANKOM TDF51 Rinse Stand, follow the steps below. 24.1 Remove the SDF bags from the instrument. 24.
Operator’s Manual 28. Determine the Ash content within the TDF residue. See the “Ash Determination” section of this manual for recommended procedures. 29. Calculate the % TDF value. % TDF Where: pg.
Operator’s Manual Protein Determination AOAC method 991.43 suggests that the Kjeldahl method be used for determining protein content. Since a large amount of acid is used in the Kjeldahl digestion process, make sure you use enough base in the Kjeldahl distillation process. IDF – Kjeldahl Method To determine the protein content within the IDF residue, follow the procedure below. 1. Cut the filter bag just above the seal. 2.
Operator’s Manual SDF / TDF – Kjeldahl Method To determine the protein content within the SDF or TDF residue, follow the procedure below. pg. 60 1. Cut the filter bag 8 -10 mm above the filter. 2. Fold the remainder of the filter bag in thirds from side to side. 3. With your Heat Sealer set to “6” (setting may vary depending on heat sealer and power source), press the arm down for 3 to 4 seconds to seal the polypropylene edge of the filter bag. Repeat if necessary. 4. 5. 6.
Operator’s Manual Ash Determination ANKOM Technology recommends that you use ceramic crucibles with fitted covers to avoid cross contamination or loss of ash. Fold the filter bags so that they easily fit into the crucibles. IDF To determine the ash content within the IDF residue, follow the procedure below. 1. Starting at the bottom of the filter part of the bag, fold the bag upward, leaving at least 10 mm at the top of the clear polypropylene that is not folded.
Operator’s Manual SDF / TDF During a TDF procedure there is no residue captured in the IDF Flow-Thru bag. Therefore, the ash procedure is only required for the SDF filter bag. DE used in the precipitation process can sometimes be difficult to fully rinse from the walls of the polypropylene bags. Therefore, ANKOM Technology recommends that the entire SDF bag be ashed. To determine the ash content within the SDF / TDF residue, follow the procedure below. 1.
Operator’s Manual Productivity Enhancement Start a new procedure while a previous one is in progress To enhance the productivity of your instrument, you can begin the digestion process (run in the IDF part of the instrument) of a new procedure (IDF/SDF or TDF) while the precipitation process (run in the SDF part of the instrument) of a previous procedure (IDF/SDF or TDF) is still running.
Operator’s Manual To start a new assay immediately after an IDF process completes, follow the steps below. 1. Prepare samples and configure the instrument for a new digestion. Refer to the IDF/SDF Analysis and TDF Analysis sections of this manual, following all steps except for those that deal with the precipitation process (SDF). This will include interaction with the Touch Screen Display to verify the instrument set-up (as shown in the IDF/SDF Analysis and TDF Analysis sections).
Operator’s Manual 3. Install new SDF bags on the ANKOMTDF Dietary Fiber Analyzer and continue the run. When an SDF process completes while an IDF process is in progress, the following screen is displayed. The Method & Procedure shown in the top right corner is the one that is running in the top bag.
Operator’s Manual Expert Mode – save some set-up time At the beginning of each new run, the instrument gives the user step-by-step instructions for set-up. These instructions are provided to make sure that all important steps are done before starting a run. After gaining experience with the instrument by running it over time, a user can choose to skip all set-up instructions except for “Check pH manually?” and “Filter minutes OK?” by enabling Expert Mode through Diagnostics.
Operator’s Manual Status Screen The screen below shows the primary sections of the instrument Status screen when running an assay. This screen is NOT displayed when running Diagnostics. The Status screen includes sections that present information that cannot be changed by the user, along with buttons that allow the user to modify the current operations or get additional information. For explanation purposes, we have placed a number in each section of the screen and white borders around boxes 1 and 2.
Operator’s Manual 7. Bottom process – Name Box #7 always displays “SDF” to represent an SDF process within an IDF/SDF procedure or the precipitation process within a TDF procedure. 8. Bottom process – Progress Box #8 displays the actual time remaining for the bottom process. 9. Bottom process – Action Box #9 displays different detailed actions as they occur during the process that is currently running in the bottom section of the instrument.
Operator’s Manual Temperature Monitor (°C) ← Paddle 1 Paddle 2 Paddle 3 Paddle 4 Paddle 5 Paddle 6 XX XX XX XX XX XX In-line Heater XX The values shown on this screen will dynamically change as the temperatures change. When the Display Pressures button is pressed, the following screen is displayed. Pressure Monitor (psi) ← Line 1 Line 2 Line 3 Line 4 Line 5 Line 6 xx.x xx.x xx.x xx.x xx.x xx.x Input Pressure Vacuum Sensor xx.x xx.
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Operator’s Manual Fault Handling When the instrument detects a problem, it generates a fault. Some faults require the instrument to abort operations. Other faults will allow the instrument to continue operation. The computer clears all fault codes at the beginning of each new procedure. However, if you start a new procedure without correcting the cause of a fault, it will continue to re-appear. The screen below shows the primary sections of the instrument fault screen.
Operator’s Manual # Name Description Result Correction E4 In-line Heater Over Temp The device that heats the fluid before delivery is reporting a temperature that is higher than the specified range. In-progress processes are aborted. Run diagnostics. Replace sensor or heater. E5 Paddle Heater Under Temp One of the Paddle Sensors is reporting a temperature lower than the specified range. IDF process continues. SDF process unaffected. Run diagnostics. Replace Paddle Bar assy (TDF24).
Operator’s Manual Diagnostics Mode To help solve problems that may occur while running the ANKOMTDF Dietary Fiber Analyzer, and to assist with periodic maintenance procedures, a computer controlled Diagnostics Mode is available through the Touch Screen Display. Select a Function AOAC 991.43 IDF/SDF AOAC 985.29 TDF AOAC 991.43 TDF Diagnostics To access the Diagnostics Mode, press the Diagnostics button on the screen above. The following screen will be displayed.
Operator’s Manual The value on the Mixer Duty Cycle screen is only used during Diagnostics and in an AOAC 2009.01/2011.25 assay during the 16 hour Amylase/AMG digestion and Trizma phases. The default value for the specified AOAC 2009.01/2011.25 phases is 50. Heater Test When you press the Heater Test button on the Diagnostics screen, the following screen will be displayed.
Operator’s Manual Motor Test When you press the Motor Test button on the Diagnostics screen, the following screen will be displayed. Motor Test ← Motor Speed 120 RPM 240 RPM Amount (ml) 10.0 Set Valves Max Vacuum (psi) Motor Direction (FWD) 00.0 GO This diagnostic allows you to test the motor at different speeds (default is 240 RPM) and directions using different amounts of fluid.
Operator’s Manual Digestion Times When you press the Digestion Times button the following screen will be displayed. AOAC 991.43/985.29 Digestion Times ← (Press button with number to change) Amylase Digestion Time (minutes) Protease Digestion Time (minutes) HCl/AMG Digestion Time (minutes) 20 30 30 SDF Precipitation Time (minutes) Save to Permanent Memory Reset to official method times 60 This diagnostic allows you to modify the times used by the computer for the digestion processes.
Operator’s Manual When you press one of the Continue buttons, the Pump Tube Test screen is once again displayed with the bottom part of the screen showing the status of each tube. This diagnostic is automatically executed at the beginning of each new IDF/SDF or TDF run. Temperatures When you press the Temperatures button on the Diagnostics screen, the following screen will be displayed.
Operator’s Manual Clear IDF/SDF Lines When you press the Clear IDF/SDF Lines button on the Diagnostics screen, the IDF and SDF lines are cleared of any fluids. Before running this diagnostic, place small waste cups at each IDF and SDF station under the nozzles and close the Front Cover of the instrument. Expert Mode When Expert Mode is “ON,” all of the question screens in the IDF/SDF and TDF procedures are bypassed except for the “Check pH manually?” and “Filter minutes OK?” screens.
Operator’s Manual Service Mode – IDF Count When you press the IDF Count button on the Service Mode screen, the following screen will be displayed. ← IDF Count Number of IDF runs XX From this screen, you can see the number of digestion operations that have been done on this instrument. Service Mode – AOAC 2009.01/2011.
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Operator’s Manual Periodic Maintenance Inspect and clean the delivery nozzles at each IDF and SDF station On a monthly basis (or more frequently depending on usage), follow the steps below to inspect and clean the delivery nozzles. 1. Observe the spray capability during the rinse cycle of an assay. 2. Remove any plugged nozzles by pulling and turning them (they are friction fit to the delivery tubes). 3.
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Operator’s Manual QC / Calibration Checks The ANKOMTDF Dietary Fiber Analyzer is an automated and highly integrated instrument. Utilizing an on-board computer, the instrument automatically checks its operation in multiple ways. For example, during each run the instrument automatically checks for Tube Integrity. In addition to reviewing assay results, you can determine if the instrument is functioning properly by executing the following procedures.
Operator’s Manual ← Service Mode Volume Calibration Temperature Sensor Corrections IDF Count Bypass Amylase Phase Initial Heat-up (NO) AOAC 2009.01/ 2011.25 Registration 3. Press the Volume Calibration button. The following screen will be displayed. ← Volume Calibration Calibration Time START xx.x 4. Label and weigh six small empty glass beakers. 5. Press the START button on the Volume Calibration screen above. The following screen will be displayed.
Operator’s Manual 6. Install extra plastic containers under all six IDF Delivery Nozzles to catch any fluid currently in the lines. With containers in place, press the OK button. After fluid empties into the containers, the following screen will be displayed. ← Volume Calibration Place Calibration containers under the IDF Delivery Nozzles. Press OK when done. OK 7. Remove the containers with fluid. 8. Install the empty pre-weighed beakers under all six IDF Delivery Nozzles and press the OK button.
Operator’s Manual ← Volume Calibration Final fluid delivery in progress... When the fluid delivery is complete, the following screen will displayed. ← Calibration Time xx.x Volume Calibration Enter average volume delivered by pressing the number input, entering the number, then pressing the “SET” button. 20.0 SET 9. Remove the beakers with fluid and weigh them. 10. Determine the amount of fluid delivered by doing the following.
Operator’s Manual pH required? Status Yes Overall Progress AOAC 991.43 IDF/SDF Action in progress Time remaining: IDF IDF Aborted XX:XX:XX Time remaining: SDF XX:XX:XX Vacuumsensor: sensor: AAAA AAAA Vacuum Display Temps/Pressures 5. psi psi Main Menu Press the Main Menu button. The following screen will be displayed. Select a Function AOAC 991.43 IDF/SDF AOAC 985.29 TDF AOAC 991.43 TDF Diagnostics 6. Press the Diagnostics button. The following screen will be displayed.
Operator’s Manual Service Mode ← Volume Calibration Temperature Sensor Corrections IDF Count Bypass Amylase Phase Initial Heat-up (NO) AOAC 2009.01/ 2011.25 Registration 8. Press the Temperature Sensor Corrections button. The following screen will be displayed. ← Temperature Sensor Corrections Enter the difference between actual and measured temperatures. Save to Permanent Memory Station 1 Station 2 Station 3 Uncorrected temperature values -> XX.X Station 4 Station 5 Station 6 XX.X XX.
Operator’s Manual Appendix A – Reagents (AOAC 991.43, AOAC 985.29) Use Distilled Water throughout. Solutions common to both AOAC 991.43 and AOAC 985.29 methods (a) Ethanol 95%. (b) Ethanol 78%—Place 821 ml of 95% ethanol into a 1 L volumetric flask. Dilute to volume with H2O. (c) Enzyme solutions—To make your enzyme solutions for use in the ANKOMTDF Dietary Fiber Analyzer, dilute ANKOM Concentrate enzymes (TDF80/TDF81, TDF82/TDF83, TDF84/TDF85) with H2O according to the Enzyme Dilution Table below.
Operator’s Manual Appendix B – Instrument Out-of-use Procedure The fluid lines can become obstructed when the instrument is left unused for one week or longer. To avoid fluid line obstruction, execute the following procedure BEFORE the instrument is left unused for one week or longer. 1. Flush and purge the lines. These instructions are available with additional pictures in Service Procedure 208 located at www.ankom.com. 1.
Operator’s Manual 2. Open the pump doors. 2.1 Unscrew the six acorn nuts on the back of the instrument and remove the clear back panel to access the pump area. Acorn Nuts (the top two are behind the black torsion bar) 2.2 Un-latch the two pump doors so they are open. 2.3 Reinstall the clear back panel and tighten the acorn nuts. Cable Tie Pump Doors When returning the instrument to service after a period of non-use, you must close and latch the pump doors and charge all of the lines before running.
Operator’s Manual Appendix C – De-fatting Procedure According to the AOAC 985.29E method (and referred to in the AOAC 991.43 method), any sample with a fat content ˃10% must have the fat removed before determining the amount of Dietary Fiber. In the conventional method, most labs perform the de-fatting step in the same beaker in which the enzyme digestion occurs.
Operator’s Manual 5. Soak the bags in Petroleum Ether. 5.1 Place all XT4 Filter Bags into a beaker or other container. 5.2 Pour enough Petroleum Ether in the container to cover the bags and allow them to soak in the solvent for 10 minutes. 6. Remove the Filter Bags from the container and allow the solvent to evaporate until the bags are completely dry. 7. Carefully cut the XT4 Filter Bags inside the IDF Filter Bags or the IDF Flow-Thru Bags on the TDF instrument.
Operator’s Manual Appendix D – Accessories (sold separately) Contact ANKOM Technology or an authorized dealer to order accessories. pg.
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Automation saves time and money! ANKOM Technology is an international company with products that include… A2000 Fiber Analyzer Crude Fiber (AOCS Ba 6a-05), ADF, NDF Automatically adds solutions and rinses Batch process - up to 24 samples at one time XT15 Fat Extractor Official Method AOCS Am 5-04 Fully automatic Solvent recovery at 97% or greater Batch process - up to 15 samples at one time RF Gas Production System High sensitivity pressure measurement Applications include: Biomass-to-E
