Operator’s Manual Rev F 12/08/14 2052 O’Neil Rd, Macedon NY 14502 Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.
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Table of Contents Introduction .................................................................................................................................................................. 5 Warranty ....................................................................................................................................................................... 5 Filter Bags .............................................................................................................................
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Operator’s Manual Introduction ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical laboratories around the world in the environmental, agricultural, biomass, and food industries. ANKOM Technology can provide you with products for determining or monitoring dietary fiber, detergent fiber, fat, digestibility, microbial fermentation (anaerobic or aerobic) and more.
Operator’s Manual Contact Information At ANKOM Technology we are committed to your total satisfaction and therefore always available to help you get the most from your ANKOM products. We are also very interested in any comments or suggestions you may have to help us improve. For any questions or suggestions regarding your instrument, please contact us at: pg. 6 Telephone: (315) 986-8090 Fax: (315) 986-8091 Email: service@ankom.com www.ankom.
Operator’s Manual Instrument Description General Description The ANKOMXT15 Extraction System is designed to extract Crude (Free) Fat using conventional solvents, typically petroleum ether. The compounds extracted are primarily triacylglycerols together with a small quantity of related lipids, traditionally termed “Crude Fat.” The analysis is achieved by measuring the loss of mass due to the extraction of fat/oil from the sample encapsulated in a filter bag.
Operator’s Manual Safety Precautions Flammable Hazard: Static electricity is to be avoided as it is a spark hazard. Hot Surfaces: Do not touch the extraction vessel during operation. The surface can exceed 70°C (158°F). Failure to observe this caution may result in injury. Hazardous Voltages: Do not operate the instrument with the electrical compartment open. Hazardous voltages are present during operation. Failure to observe this caution may result in electrical shock or electrocution.
Operator’s Manual Instrument Installation Site Requirements To install and operate the ANKOMXT15 Extraction System you will need the following: Adjustable wrench Chilled or tap water supply not to exceed 23°C Adequate power (see “Operating Environment” section) Drain Instrument Installation Procedure To install the ANKOMXT15 Extraction System, follow the procedure detailed below. 1.
Operator’s Manual Fat Extraction Calculations Crude Fat Calculation Crude Fat contained within a food or feed sample can be calculated using the following formula: % Crude Fat = Where: = = = W1 W2 W3 100 x (W2 – W3) W1 Original weight of sample Weight of pre-extraction dried sample and filter bag Weight of dried sample and filter bag after extraction For Liquid Samples, W2 and W3 in the equation above will include the weight of Diatomaceous Earth (DE).
Operator’s Manual Sample Preparation Sample Preparation Procedure for Crude Fat – for LOW fat samples To prepare meat samples with ≤ 15% fat by weight or plant samples with ≤ 20% fat by weight for fat extraction, follow the procedure detailed below. 1. Using a Solvent Resistant Marker, number all of the empty filter bags you will use during the fat extraction procedure.
Operator’s Manual Sample Preparation Procedure for Crude Fat – for HIGH fat samples To prepare meat samples with > 15% fat by weight or plant samples with > 20% fat by weight for fat extraction, follow the procedure detailed below. 1. Using a Solvent Resistant Marker, number all of the weigh tins and empty filter bags you will use during the fat extraction procedure. Use one weigh tin per filter bag.
Operator’s Manual Seal 11. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of the filter bag (as shown to the right). If the seal is not strong, re-seal the bag. 12. Repeat steps 1 – 11 for all filter bags that will be used in the ANKOMXT15 Extraction System. (Up to 15 bags can be processed during one procedure.) 13.
Operator’s Manual Sample Preparation Procedure for Crude Fat – for Liquid samples To prepare liquid samples for fat extraction, follow the procedure detailed below. 1. Using a Solvent Resistant Marker, number all of the empty filter bags you will use during the fat extraction procedure. Although it is not necessary in the determination of fat, it is recommended that you weigh and record the weights of all the filter bags prior to filling them in order to calculate the moisture value for the samples.
Operator’s Manual 10. Dry all samples at 102°C±2° for 3 hours to remove moisture prior to the extraction. 11. Remove the samples from the oven and place them in a Desiccant Pouch. 12. Allow the samples to cool to room temperature. This should take about 10 – 15 minutes. 13. Re-weigh each bag (W2) immediately after removing from the Desiccant Pouch. The samples are now ready for the fat extraction procedure. Rev F 12/08/14 pg.
Operator’s Manual Fat Extraction step-by-step procedure using the ANKOMXT15 To perform fat extraction on prepared samples, follow the procedure detailed below. 1. Turn the instrument power ON. The Display will briefly show the software version number followed by the screen to the right. PET Ether v The factory default solvent shown on the Display is "Pet Ether.
Operator’s Manual 3. Insert Filter Bags into the instrument. 3.1 Press down the Safety Valve Handle to release the Safety Valve Pin and rotate the Main Handle upward until it stops. 3.2 Use the Extraction Vessel Handle to pull the Extraction Vessel out. 3.3 Slide the Extraction Vessel toward you far enough to remove the white Teflon Insert or to put the Bag Holder directly into the Teflon Insert while it remains in the Extraction Vessel.
Operator’s Manual 3.6 Slide the Extraction Vessel into the instrument ensuring the Extraction Vessel O-ring stays in place. 3.7 Pull the Main Handle downward, closing and locking the Extraction Vessel in place. You will hear a click noise when the Safety Valve Pin locks in place. Before running the instrument, verify that the Extraction Vessel is locked in place by attempting to rotate the Main Handle upward. If it is not locked, repeat step #8 above. 3.8 Press ENTER on the Keypad.
Operator’s Manual 8. Determine the amount of fat that was extracted. 8.1 When the Pressure Gauge reads zero, remove the Extraction Vessel by pushing down on the Safety Valve Handle to release the locking pin and then rotating the Main Handle to the open position. 8.2 Use the Extraction Vessel Handle to remove the Extraction Vessel. Warning: The Extraction Vessel interior and top will be HOT when the extraction process is complete. 8.3 Remove the Teflon Insert from the Extraction Vessel. 8.
Operator’s Manual Periodic Maintenance Cleaning the Extraction Vessel Some compounds extracted during the fat extraction procedure can create a black residue in the bottom of the Extraction Vessel. The vessel can most easily be cleaned by using a drill with a long shafted stiff wire brush (ANKOM part # X107). This treatment will remove a lot of the black coating that accumulates on the bottom of the vessel.
Operator’s Manual Cleaning the water filter To clean the water filter (located on the water supply line), follow the procedure detailed below. 1. Unscrew the filter from the instrument. 2. Unscrew the filter clamp from the water supply line. 3. Use compressed air to blow backwards through the filter (blow opposite the arrow on the filter). If the filter is clogged, replace it with ANKOM part # X97. 4. Reconnect the filter and fittings. 5. Turn on the water and check for leaks.
Operator’s Manual Troubleshooting & Replacement Parts The ANKOM technology web site has the most current troubleshooting and replacement parts information. Therefore, if you have any questions about the operation of your ANKOMXT15 Extraction System, or if you need replacement parts, please visit our web site at www.ankom.com. pg.
Operator’s Manual Diagnostics To help solve problems that may occur while running the ANKOMXT15 Extractor, the following computer controlled Diagnostics are available and accessible through the Display and Keypad. 1. 2. 3.
Operator’s Manual 4. Press the ENTER button on the Keypad to turn ON the heater relay (Q0). The screen to the right will be displayed. Heater (Q0) ON ENTER to toggle 5. Press the ENTER button on the Keypad to turn OFF the heater relay (Q0). The screen to the right will be displayed.
Operator’s Manual Check Temperature and Level Sensors To check the Temperature and Level Sensors, follow the procedure below. 1. Access the Diagnostics screen (see the "Access Diagnostics Screen" section for details). Diagnostics ^ 2. With the Diagnostics screen displayed, press the ENTER button on the Keypad. The screen to the right will be displayed. Outputs Exit v 3. Press the Down Arrow button (v) on the Keypad. The screen to the right will be displayed.
Operator’s Manual 7. Press the Up Arrow button (^) on the Keypad to exit Diagnostics. Press the Up Arrow button (^) on the Keypad up to three more times to return to your solvent screen (example shown to the right). PET Ether v Check the state of the ETS (Emergency Temperature Shut-off switch) relay To check the state of the ETS relay, follow the procedure below. pg. 26 1. Access the Diagnostics screen (see the "Access Diagnostics Screen" section for details). Diagnostics ^ 2.
Operator’s Manual Appendix A – AOCS Official Fat Extraction Procedure ANKOM Technology Method AOCS Official Procedure Am 5-04 Rapid Determination of Oil/Fat Utilizing High Temperature Solvent Extraction Definition This method determines crude fat by extracting with petroleum ether. The compounds extracted are predominantly triacylglycerides. Small amounts of other lipids along with minor compounds having some solubility in petroleum ether are also extracted.
Operator’s Manual Numbered Notes 1. Pre-extraction drying time of the samples in the filter bags can be greatly reduced by using a microwave oven. However, microwave ovens vary in energy output and samples vary in water and fat composition. You will need to experiment with your microwave oven to determine the proper conditions for use. Contact ANKOM Technology for further information. 2.
Operator’s Manual Appendix B – Electrical Diagram (pg. 1 of 3) Rev F 12/08/14 pg.
Operator’s Manual Appendix B – Electrical Diagram (pg. 2 of 3) pg.
Operator’s Manual Appendix B – Electrical Diagram (pg. 3 of 3) Rev F 12/08/14 pg.
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