The information in this document is subject to change without notice and should not be construed as a commitment by ORFLO Technologies, a DBA of E.I. Spectra, LLC. Neither Orflo Technologies nor any of its affiliated corporations assumes responsibility for any errors that may appear in this document. This manual is believed to be complete and accurate at the time of publication.
Contents Introduction ................................................................................................................................ 1 Symbols Used in This User Guide.............................................................................................. 2 Safety Precautions ..................................................................................................................... 3 Consignes de sécurité.......................................................................
Warranty ...................................................................................................................................
Introduction The ORFLO Moxi Z Mini Automated Cell Counter performs cell count and size measurements for particle sizes of 2 – 34 microns. It also provides an assessment of mammalian culture viability using a proprietary software algorithm to report the standardized Moxi Viability Index (MVI). It combines the gold standard Coulter Principle with a patented thin-film sensor technology to deliver highly accurate and repeatable results in just 8 seconds.
Symbols Used in This User Guide The following symbols are used throughout this user guide and/or on product labels. The user is responsible for operating the product in accordance with the indicated requirements: Symbol / Symbole Definition / Définition Warning alerts you to actions that may cause personal injury or pose a physical threat. La mise en garde vous alerte des actions qui risquent de causer des blessures corporelles ou de constituer une menace physique.
Safety Precautions Please review and understand the safety instructions below before operating the Moxi Z Cell Counter. WARNINGS: To avoid danger of electric shock, do not install the instrument in an area with a high humidity level, such as a greenhouse or an incubator. Refer to Operating Environmental Conditions in Specifications section. Do not touch the USB cable or USB charging adapter with wet hands.
Consignes de sécurité Veuillez lire et vous assurer de comprendre les consignes de sécurité ci-dessous avant d’utiliser le compteur Moxi Z Cell. AVERTISSEMENTS : Afin d’éviter tout danger de choc électrique, ne pas installer l’instrument dans un endroit où le taux d’humidité est élevé, comme dans une serre ou un incubateur. Se reporter à la section sur les spécifications en matière de conditions environnementales d’exploitation.
Moxi Z Mini Automated Cell Counter Kit The Moxi Z Mini Automated Cell Counter kit includes the Moxi Z instrument, USB Cable, Power Adapter (US and EU versions only), Cassette Dispenser, Calibration Check Beads, USB Flash Drive with software/user guide and 25 Cassettes (2 tests per cassette).
Part Function Touch Screen Display Allows user to interface with instrument. Displays all information needed for operation. Displays test results and histograms with curve fitting, gating, cell volume, concentration, diameter, and Moxi Viability Index (MVI). Power/Reset Button Turns Moxi Z on and off. Resets the unit when pressed and held for >5 seconds. Cassette Tray Tray that needs to be pressed down for inserting cassette USB Cable Port Connects instrument to USB cable.
Part Function Cassette Dispenser Stores up to 25 cassettes for convenient dispensing USB Cable Connects instrument to PC/Mac or power adapter Power Adapter (US and EU models only) Connects USB cable to an AC outlet USB Flash Drive Stores Moxi Z software and user manual Calibration Check Beads Polystyrene beads for confirming proper system operation and calibration Electronic Calibration Cassette (sold separately) Electronic cassette for verifying proper system operation and calibration Install
Note: Detergents and DMSO may interfere with cell counting. 10% DMSO is not an acceptable diluent but 1% DMSO may be used. Serum-enriched media may also interfere with counting. Dilute samples with ORFLO Diluent or PBS rather than serum-enriched media. Materials Required Cell sample (diluted, if necessary); 75 µL minimum. Pipette and appropriately sized pipette tips Using the Moxi Z Automated Cell Counter Settings Set the date and time by pressing the Settings icon on the main menu of the Moxi Z.
4. Pipette a 75 µL sample into the fill port of the cassette (either test 1 or test 2, depending on which end of the cassette was inserted into the instrument). 5. For counting most mammalian cells, touch the screen anywhere to start. For counting very small particles (4 to 8um in diameter), the Moxi Z can be run in Small Particle Mode. In this mode, Moxi Z sets the diameter scale to 2 to 10um as the default and performs the count using optimized parameters for the detection of small cells.
gold standard in counting technologies. Moxi Z gating-mode does implement elements of the Moxi Z coincidence correction algorithm, but without the full curve-fitting technique, it does not achieve quite the same level of accuracy. As a result, Orflo recommends the use of the curve-fitting count mode over gating mode whenever possible in order to generate the “true” cell count. 1. Except when running in Small Particle Mode, the results of a test are initially displayed on a diameter scale of 2-34 µm.
Retrieving and Deleting Data 1. To open a saved test, press the Histogram icon on the Home screen. 2. Icons for up to nine saved histograms will be displayed on the screen. Press the appropriate icon for the test of interest or press the Page Up or Page Down icon to view more test results. 3. Each histogram will be opened in either the Curve Fit Count mode or Gated Count mode, depending on how it was saved.
5. Press the Delete icon to permanently delete the results of the test. 6. Press the Done icon to close the test results and return to the Home screen. (note: zoomed view will not be saved) Moxi Z Performance Test Using Calibration Check Beads A 5 mL sample of Calibration Check Beads (Cat. No. MXA005) is included with each MOXI Z Automated Cell Counter. The calibration check beads consist of polymer microspheres of a known size in solution at a known concentration.
Moxi Z Performance Test Using Electronic Calibration Cassette The Electronic Calibration Cassette (Cat. No. MXA004) is an optional accessory that can be used to verify proper operation of the MOXI Z. Simply insert the Electronic Calibration Cassette (ECC) into the MOXI Z and the test will begin automatically. The ECC will generate data that simulates various cell sizes and concentrations. Upon completion of the test, a pass or fail result will be displayed as shown.
Installing MoxiChart Software on a PC 1. Insert Moxi Z flash drive into a USB port on your computer. 2. If prompted to run MoxiChart Install, click YES and follow the on screen instructions. Otherwise, open ORFLO DISK folder and double click on the file MoxiChart_Win_Install.exe. Complete the setup by following the instructions of the MoxiChart Setup Wizard. Connecting to Moxi Z via Bluetooth with a PC 1. Press the Bluetooth icon to display the unit’s Bluetooth I.D.
2. Run MoxiChart application and click on Bluetooth icon in upper right corner. 3. Choose the device which corresponds to the Moxi Z. 4. Select a folder to upload data files to.
Installing MoxiChart Software on a Mac 1. Insert Moxi Z flash drive into a USB port on your computer. 2. Open the folder for the USB drive. 3. Double click on the on the file MoxiChartInstaller.dmg 4. Drag and drop MoxiChart into your applications folder. 5. From the applications folder, select MoxiChart to launch the application. Connecting to Moxi Z via Bluetooth with a Mac The procedure for connecting Moxi Z via Bluetooth with a Mac is the same as that for a PC.
Updating Moxi Z Firmware The Moxi Z firmware can be updated through a Bluetooth connection. The user first needs to put the Moxi Z into a firmware update mode. This can be done in either of the following two ways: With the unit powered off, quickly press the power/reset button four times. When successful, the white Moxi Z screen will display with text under the Moxi Z logo that indicates the system is in firmware update mode. If unsuccessful, turn the power off and retry.
Troubleshooting Symptom Cause Corrective Action Questionable concentration Concentration of cell sample is too high or too low Make sure concentration of cell sample is within recommended guidelines. Refer to General Guidelines section. Wrong diluent Use a diluent that is compatible with cells being counted. ORFLO Diluent (Cat No. MXA006) or equivalent. Cell clumping Ensure the cells are in a single-cell suspension. Break clumps by pipetting up and down with a standard pipette.
Error/Warning Messages ---------------------------------Messages d’erreur/de mise en garde Cause -----------------------Cause Corrective Action ----------------------------------------------------Mesure corrective Viscous sample (e.g. serum-enriched media) Use ORFLO Diluent or PBS to dilute sample. Cell Counter sensor is blocked. Sample concentration is too high; dilute cell sample more. Ensure that cells are in a single cell suspension. Break clumps by pipetting up and down with a standard pipette.
Error/Warning Messages ---------------------------------Messages d’erreur/de mise en garde Defaults loaded False start Cause -----------------------Cause Corrective Action ----------------------------------------------------Mesure corrective Instrument has detected corruption or new version of firmware and reset all settings to factory defaults If problem persists, return instrument for service. Sensor malfunction Use new sensor.
CAUTION: When sanitizing, make certain that no liquid enters any internal cavities of the instrument. MISE EN GARDE : Lors de la décontamination, s’assurer qu’aucun liquide ne pénètre dans les cavités internes de l’instrument. Maintenance There is no routine maintenance required for the Moxi Z Automated Cell Counter. In addition, there are no user serviceable parts. Instrument repairs must be carried out by authorized personnel only.
Specifications for the Moxi Z Automated Cell Counter Performance Sample Volume Required Dynamic Range Average Cell Size Average Cell Volume Cell Concentration Test Time Viability Assessment for mammalian cultures Software Dimensions Length Width Height Weight Electrical Specifications Internal Battery AC Power Adapters (US and EU types) 75 µL 2 - 34 µm 4 - 25 µm diameter 34 - 8,180 fL 3,000-500,000 cells/mL 100,000-300,000 cells/mL recommended for optimal MVI calculation 8 seconds Moxi Viability Index (M
Ordering Information This section lists catalog numbers for the Moxi Z Automated Cell Counter and related products. You can purchase Orflo products through a regional distributor or on-line at www.orflo.com. See the Technical Assistance section for information about contacting Orflo. Product Description Cat. No. Moxi Z Mini Automated Cell Counter Kit, U.S. Version.
Technical Service For technical service, contact ORFLO Technologies at 855-TRY-MOXI (855-879-6694) or email us at info@orflo.com. Warranty ORFLO Technologies (“Orflo”) warrants that its products will meet their applicable published specifications when used in accordance with their applicable instructions for a period of one year. ORFLO MAKES NO OTHER WARRANTY, EXPRESS OR IMPLIED. THERE IS NO WARRANTY OR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.
