Thermo Scientific Dionex UltiMate 3000 Series Variable Wavelength Detectors VWD-3100 and VWD-3400RS Operating Instructions (Original Operating Instructions) Revision: 1.7 Date: September 2013 © 2013 Thermo Fisher Scientific Inc. Doc No. 4820.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Operating Instructions
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Declaration of Conformity ( O r i g i n a l D e c l a r a t i o n o f C o n f o r m i t y) Product: Types: Thermo Scientific Dionex UltiMate 3000 - Detector VWD-3100 and VWD-3400RS Dionex Softron GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: • Low-Voltage Directive 2006/95/EC • EMC Directive 2004/108/EC The electrical safety of the products was evaluated
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UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Table of Content 1 Introduction................................................................................................................... 1 1.1 How to Use this Manual ............................................................................................ 1 1.2 Safety ......................................................................................................................... 3 1.2.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3.5.2 Installing the Detector ...................................................................................... 43 3.5.3 Configuring the Detector ................................................................................. 44 3.6 Setting Up the Detector in DCMSLink .................................................................... 48 4 Preparation for Operation (Startup) ................................................
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7 Service ........................................................................................................................ 115 7.1 General Notes and Safety Precautions ................................................................... 115 7.2 Wavelength Calibration and Verification .............................................................. 117 7.3 Lamp ..................................................................
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Page iv Operating Instructions
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 1 Introduction 1.1 How to Use this Manual The layout of this manual is designed to provide quick reference to the sections of interest to the reader. However, in order to obtain a full understanding of your Thermo Scientific™ Dionex™ detector, Thermo Fisher Scientific recommends that you review the manual thoroughly before beginning operation.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors All rights reserved, including those for photomechanical reproduction and storage on electronic media.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 1.2 Safety The CE Mark label and cTUVus Mark safety label on the instrument indicate that the detector is compliant with the related standards. 1.2.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors At various points throughout the manual, messages of particular importance are indicated by certain symbols: Tip: Indicates general information, as well as information intended to optimize the performance of the instrument. Important: Indicates that failure to take note of the accompanying information could cause wrong results or may result in damage to the instrument.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 1.2.2 Safety Precautions When working with analytical instrumentation, you must know the potential hazards of using chemical solvents. Tip: Before initial operation of the detector, make yourself familiar with the contents of this manual. For the safety precautions in French, see page 9.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors • Hazardous gases Install the HPLC system in a well-ventilated laboratory. If the mobile phase or sample includes volatile or flammable solvents, do not allow them to enter the workspace. If the mobile phase or sample includes volatile or flammable solvents, avoid open flames and sparks. • Electrostatic discharge Discharge of electrostatic energy may lead to sparking and can constitute a fire hazard.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors • Hot surfaces ♦ During operation, the lamps and the surrounding parts become extremely hot and remain so for some time after the detector is turned off. To avoid possible injury, allow sufficient time for the lamp to cool down after turning off the detector. Only then start with the maintenance and repair work. ♦ Flow cells can become extremely hot during operation.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors • Before switching from buffer to organic solution, rinse the analytical system thoroughly with deionized or HPLC grade water. • When switching to another solvent, ensure that the new solvent is miscible with the one contained in the HPLC system. If the solvents are not miscible, the system can be damaged, for example, by flocculation. • If a leak occurs, turn off the instrument and remedy the situation immediately.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 1.2.3 Consignes de Sécurité Si vous utilisez d'instrumentation analytique, vous devez connaître les risques d'utilisation de produit chimiques. Veuillez noter : Avant de commencer à utiliser l'instrument, assurez-vous que vous vous êtes familiarisés avec le contenu de ce manuel.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors • Gaz dangereux Installez le système HPLC dans un laboratoire bien ventilé. Si la phase mobile ou l’échantillon contient des solvants volatils ou inflammables, vous devez assurer qu'ils ne pénètrent dans l'espace de travail. Si la phase mobile ou l’échantillon contient des solvants volatils ou inflammables, évitez les flammes nues et les sources d’étincelles à proximité.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors ♦ Portez des lunettes de protection lorsque vous manipulez des capillaires en silice fondue (pendant l'installation, découpe, etc.). • Surface chaude ♦ Lampes et les parties environnantes deviennent très chaudes pendant le fonctionnement. Pour éviter toute blessure, vous attendez après mise hors tension jusqu'à ce que les lampes soient refroidies. Commencer seulement alors les travaux d'entretien.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors • Remplacez les cordons d'alimentation électrique défectueux. Utilisez uniquement les cordons d’alimentation électrique spécifique à l’instrument. • Utilisez seulement des pièces de rechange originales et des accessoires autorisés par Thermo Fisher Scientific. • Réglez toujours une limite de pression minimum pour le système HPLC. Ceci prévient les dommages résultant de fuites ou du fonctionnement à sec de la pompe.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 1.3 Intended Use For Research Use Only. Not for use in diagnostic procedures. The device is designed to be operated only be qualified and authorized personnel. All users must know the hazards presented by the device and the used substances. The detector is designed for laboratory research use in high-performance liquid chromatography (HPLC) applications.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors If there is any question regarding appropriate usage, contact Thermo Fisher Scientific before proceeding. Warning: If the device is used in a manner not specified by Thermo Fisher Scientific, the protection provided by the device could be impaired. Thermo Fisher Scientific assumes no responsibility and will not be liable for operator injury and/or instrument damage.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 1.4 Federal Communications Commission (FCC) Note This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the U.S. FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
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UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2 Overview 2.1 Unit Description The detector is a high-quality instrument designed for HPLC analysis as part of the UltiMate 3000 system. • The detector is a dual-beam, variable wavelength photometer with one measurement and one internal reference beam.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.2 Operating Principle Photometric detection is based upon the absorption of monochromatic light. The degree of absorption depends on the sample molecule, its concentration, the light's path length in the sample, and the measurement wavelength. The definition of absorbance is Lambert-Beer's Law.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Fig. 1: Optics setup No. Component Description 1 Tungsten lamp Light source for the visible and near-infrared wavelengths (345 nm 900 nm) 2 Mirror (VIS) Focuses the light from the tungsten lamp to the through hole (aperture) of the deuterium lamp. 3 Deuterium lamp Light source for the UV wavelengths (190 nm - 670 nm) 4 Mirror Focuses the combined light of both lamps to the entrance slit.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.3 Configurations The detector is available in the following configurations: Detector Description Part No. VWD-3400RS detector for measurements up to 200 Hz*. Up to four wavelengths can be recorded simultaneously. The detector is shipped with a deuterium- and a tungsten lamp. 5074.0010 VWD-3100 detector for measurements up to 100 Hz. However, only one wavelength can be recorded. The detector is shipped with a deuterium lamp.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors In addition, the following is available from Thermo Fisher Scientific for the use of the detector in an UltiMate 3000 XRS system: * Description Part No. UV monitor for the XRS system, ultra-low dispersion (cell volume: 45 nL, cell material: fused silica, path length: 10 mm*, pressure limit: 30 MPa) Recommended for the use in an UltiMate 3000 XRS system in combination with a mass spectrometer.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.4 Interior Components The front panel door tilts downward to provide easy access to the inside front panel, for example, for maintenance and repair work. Important: The open front panel door is not designed to carry weight. Therefore, you should not place any objects on the open door. Important: Ne placez aucun object lourd sur la porte ouverte du panneau avant. 1 1 2 3 4 Fig.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.5 Front Panel Elements 3 2 1 Fig. 3: Front panel view No. Element Function 1 Display Shows information about the detector: - General information upon power-up (→ page 56) - Status screen, depending on the detector configuration (→ page 57) - Various function keys and menus (→ page 64) - Messages (→ page 95) 2 Standby button Switches the detector to Standby mode (the LED is lighted).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.6 Rear Panel 1 2 3 4 5 6 7 Fig. 4: Rear panel (here with DAC board) No.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.6.2 Fuse Cartridge The fuse cartridge contains two slow-blow fuses, rated at 2 A (5 x 20 mm). For information about how to change the fuses, see page 132. 2.6.3 USB Port The Chromeleon Chromatography Management System can use a USB connection to control the detector. Data is transferred digitally via the appropriate USB cable (→ page 37). To ensure trouble-free operation, use only the cables shipped with the detector.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.6.6 pH and Conductivity Monitor (optional) A pH and Conductivity Monitor (part no. 6082.2005) is available as an option, consisting of a suitable plug-in extension board for installation in the detector, a flow cell carrier with pre-mounted pH and conductivity flow cells, a hood, and a pH electrode. For installation and configuration details, refer to the PCM-3000 manual.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.7 Fluid Connections The detector is designed to provide easy access to the fluid components. Tilt the front cover downward. At dedicated positions in the front part of the enclosure, six slots are provided for the capillaries: on the top and bottom of the enclosure (two slots each), and on the left and right side of the enclosure (one slot each) (→ Fig. 2, page 22).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.8 Flow Cells All flow cells (→ page 20) are optimized for fast separations with no loss in chromatographic resolution. An identification chip is fitted to the flow cell in the factory. The chip stores unique information about the flow cell, including the cell type and serial number. When the flow cell is installed, a contact on the flow cell connects the chip to the detector electronics.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.9 Lamps The following lamps are available for the detector: • A deuterium lamp for UV wavelength detection • A tungsten lamp for visible and near-infrared wavelength detection The table lists the lamps to use for various wavelength detection ranges. Wavelengths Detected Lamp < 345.0 nm Deuterium < 670.0 nm Tungsten Between 345.0 and 670.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.10 Leak Sensor A leak sensor (→ Fig. 2, page 22) is installed inside the detector. If liquid collects in the drip tray under the fluid connections, the leak sensor reports a leak, and the Status LED on the front panel door changes to red. It depends on the leak sensor mode setting whether a message appears on the front panel display and in the Chromeleon Audit Trail and a beep alerts you (→ page 82).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Two modes of software control are available: • Direct Control With direct control, you select operating parameters and commands in the Commands (F8) dialog box. Direct commands are executed as soon as they are entered. For routine operation, most parameters and commands are available also on a control panel. For more information about direct control, see page 59.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2.12 System Wellness, Predictive Performance, and Diagnostics System Wellness monitors the health of the detector.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3 Installation 3.1 Facility Requirements The installation site must meet the following requirements: • The main power switch and the main power receptacle are on the rear panel. Make sure that ♦ Free and unrestricted access to the main power switch is ensured at all times. ♦ The power cord of the device can be easily reached and disconnected from the power line at all times.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2. Grasp the detector by the sides. Slowly and carefully, pull the instrument out of the shipping container and place it on a stable surface. Important: To prevent the device from falling, grasp the device by the sides, and then lift the unit together with the foam spacers out of the shipping container. Do not lift the device by the packaging material or the front panel.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3.3 Positioning the Detector in the UltiMate 3000 System If the detector is part of an UltiMate 3000 system, for example for analytical HPLC applications, you should stack the individual modules, for example, as shown in Fig. 9 and interconnect them on the rear panel as shown in Fig. 10. However, the arrangement of the system modules depends on the application.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Solvent rack Pump (here RS pump) Autosampler Column compartment VWD Fig. 10: Example for the rear panel connections on an UltiMate 3000 system Apart from the Solvent Rack, all modules of the UltiMate 3000 system can be connected separately to the Chromeleon computer by using the USB port on the rear panel of the instrument.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3.4 Connecting the Detector 3.4.1 General Information If you want to operate the detector with Chromeleon Verify that Chromeleon is installed on the computer and that the license code is entered before you connect the detector to the USB port on the Chromeleon computer and turn on the detector power.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3.4.4 Connecting the Signal Cable (Digital I/O) To connect external devices to the digital I/O ports, use the appropriate mini-DIN cables (part no. 6074.0001). 1. Plug the 6-pin connector of the mini-DIN cable into the Digital I/O port A (or B). 2.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 4. Slide the left guide bar of the DAC board onto the green edge next to the USB port. USB Port Guide bar Fig. 12: Installing the DAC board 5. Push the board partially into the enclosure. Apply slight pressure between the two analog outputs to push the board further into the enclosure until it locks into position. 6. Attach the board to the rear panel, by using the 4 screws as shown in Fig. 11. 7.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3.4.5.3 Configuring the Analog Outputs Make the required settings for the analog outputs in Chromeleon or on the front panel display. To configure the analog outputs in Chromeleon 1. On the Detector page in the Properties dialog of the detector, verify that the DAC check box is selected (→ page 45). When the check box is cleared, the analog outputs will not be available in Chromeleon. 2.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3.5 Setting Up the Detector in Chromeleon This section provides brief instructions for setting up the detector in Chromeleon. For details, see the Chromeleon Help. Tip: When the detector is connected to the Chromeleon computer, verify that the Chromeleon software is installed before turning on the detector power for the first time.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7. Windows Vista, Windows 7, and Windows Server 2008 will automatically detect the new detector and perform the USB installation. If Windows fails to detect the detector and launches a wizard instead, this indicates that you connected the detector to the computer and turned on the power for the first time before you installed Chromeleon. To resolve the problem: a) Click Cancel to exit the wizard. b) Turn off the detector.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3.5.2 Installing the Detector After the USB software for the detector has been installed (→ page 41), install and configure the detector in Chromeleon: 1. Start the Chromeleon Server Monitor (→ page 41) and the Chromeleon server if they are not yet running. 2. Start the Chromeleon Server Configuration program by clicking Start on the taskbar.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3.5.3 Configuring the Detector 3.5.3.1 Initial Installation On each configuration page for the detector, check and change the settings if necessary and select additional settings if needed. You may reopen the configuration pages later again to change the settings (→ page 47).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors • Module Address Select the module address of the detector. Click Browse and then double-click the detector that you want to use on the Device List. The address is automatically entered in the Module Address box. Chromeleon connects to the detector and transfers the settings from the detector firmware to Chromeleon, setting the options on the pages accordingly.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors ♦ UV Lamp and VIS Lamp The UV Lamp check box is selected by default. Select the VIS Lamp check box if the detector is fitted also with a tungsten lamp. • Expansion Board ♦ DAC Verify that the DAC check box is selected when the DAC board is installed (→ page 38). If it is not, the analog outputs will not be available in Chromeleon (→ page 40).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3.5.3.2 Changing the Configuration Properties You may reopen the configuration pages later again to change the settings. 1. Start the Server Configuration program (→ page 43). 2. Right-click the detector in the timebase and select Properties on the menu. 3. Change the settings as needed. For a description of the pages, see section 3.5.3.1 (→ page 44). 4.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3.6 Setting Up the Detector in DCMSLink To set up the detector in DCMSLink, refer to DCMSLink Installation Guide, which is provided on the DCMSLink DVD in the Additional Documents\DCMSLink User Documents folder. 1. Install and configure the DCMSLink software (→ DCMSLink Installation Guide). 2. Open the Chromeleon Server Configuration program (→ DCMSLink Installation Guide). 3.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 4 Preparation for Operation (Startup) 4.1 Overview of Actions Important: Two locks on the bottom of the detector secure the optics during shipment (→ Fig. 8, page 34). Loosen these locks before you operate the detector for the first time. Important: Deux vis sur le dessous de détecteur fixent le systeme optique durant le transport (→ Fig. 8, page 34).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 4.2 General Precautions for Connecting Capillaries When connecting capillaries, observe the following general precautions: • Observe the precautionary statements for capillaries and capillary connections in section 1.2.2 (→ page 5). • UltiMate 3000 systems with stainless steel fluidics are shipped with Viper capillary kits.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 4.3 Connecting Drain Tubing To discharge liquid leaks and waste, the detector has a drain port at the bottom right of the instrument. Drain port Fig. 15: Drain port Direct liquid leaks to waste through the drain system of the UltiMate 3000 system. Direct liquid leaks to waste via the drain system of the UltiMate 3000 system, using the components from the drain kit.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 4.4 Equilibrating the System Before using the detector for sample analysis, equilibrate the UltiMate 3000 system: 1. Pump the starting solvent through the entire system until the system is free of any other liquid composition. 2. Heat or cool all temperature-controlled devices, such as the column oven, to the temperature required for the application. 3. Set the detector wavelengths and turn on the lamps.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors The equilibration panel shows the equilibration status of each instrument in the system. Fig. 16: Equilibration window To equilibrate the system from the front panel menus Select and perform the operating commands and parameters on the front panel menus of the instruments. For information about the detector menus, see section 5.4.2.1 (→ page 67).
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UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5 Operation and Maintenance For information about how to control the detector via the Chromeleon Chromatography Management System, see section 5.3 (→ page 58). In addition, function keys and menus are available on the front panel display to facilitate operation during, for example, initial installation of the detector, diagnostics, and maintenance.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.1 Power-Up To start the detector for the first time, turn on the main power switch on the rear panel of the detector. The following sequence of events occurs when the detector is powered u p: • For a short time, general information about the detector appears on the detector display: device type, serial number, bootloader and firmware versions. • The detector runs a series of internal tests. (The test takes about 30 seconds.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.2 Status Screens When the self test was successful, the initial screen changes to the status screen. The number of measurement channels determines the appearance of the screen. Whenever the detector is turned on, the last status screen before the power was turned off is restored Wavelength [nm] 254 UV: On VIS: Off Mode: Single wvl.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.3 Detector Control 5.3.1 Operation from the Front Panel Display The function keys provided on the front panel display (→ page 64) allow you to operate the detector directly from the display. Select the required settings on the front panel menus (→ page 67), and then start data acquisition as described in section 5.5.5 (→ page 80).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.3.2.1 Connecting to Chromeleon 1. Start the Chromeleon Server Monitor and the Chromeleon server if they are not yet running (→ page 41). 2. Start the Chromeleon client by clicking the Chromeleon icon on the desktop. If the Chromeleon icon is not on the desktop, click Start on the taskbar, point to Programs (or All Programs, depending on the operating system), point to Chromeleon, and then click Chromeleon. 3.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3. Press the F8 key or select Command on the Control menu. 4. To see the parameters and commands that are available for the detector, click the plus sign next to UV. The commands and parameters available in the dialog box vary, depending on the ♦ Chromeleon version ♦ Options selected for the detector in the Properties dialog (→ page 44). ♦ Display filter level (Normal, Advanced, or Expert) 5.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors To open a control panel 1. On the View menu, click Default Panel Tabset or click the corresponding icon on the toolbar , and then connect to the Chromeleon server. Chromeleon creates centralized control panels, called panel tabsets (→ Fig. 19) for all timebases available on the Chromeleon server.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.3.2.3 Automated Control With automated control, you create a program file (PGM) for automated operation of the detector. Programs can be created automatically with the help of a software wizard or manually by editing an existing program.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 2. Change the settings in the program as desired. The easiest way is to edit a program is to do this in the Device Views (→ Fig. 20). Click a device icon and change the settings on the device pages. Editing the program in the Device Views ensures correct command syntax. If you cannot edit a certain parameter in the Device View, click Commands to open the Commands View.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.4 Display Screens (Function Keys and Menus) Function keys and menus are available on the detector display. Thus, you can make several settings or execute certain commands directly from the detector. For information about the function keys, see section 5.4.1 (see further down) and page 67. For information about the menus, see section 5.4.2.1 (→ page 67). 5.4.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors To … Select… Open the Main menu (→ page 69) Menu Open the Control menu.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.4.2 Detector Menus Fig. 23 shows an overview of the detector menus. For information about the general menu layout and structure, see page 67. For information about the commands and parameters that are supported by the menus, see sections 5.4.2.2 through 5.4.2.6 (→ page 69 and following pages). Main 1. Control 2. Preferences 3. Diagnostics 4. Configuration 1. Wavelength 1 1. Multi wvl. data rate 1. Checks 1.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.4.2.1 General Menu Layout and Structure In general, the menu layout is as follows: 1 2 3 > Control (1..4) 1. Wavelength 1 2. Wavelength 2 3. Wavelength 3 4. Wavelength 4 ∨ ∧ Select Back Fig. 24: Menu layout (here Control menu) No. Description 1 Reports the menu name and the number of items on the menu list. 2 The menu items appear on a list and are numbered consecutively. The selected item is underlined.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors To … Select … Toggle between two operating states (for example, between On and Off). Toggle Confirm the selection and perform the action. For example, if you change the operating status of a lamp from Off to On, you have to select OK to confirm the change and turn on the lamp. OK Cancel the action and restore the last value.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.4.2.2 Main Menu The Main menu provides top-level access to the menu structure. To open the Main menu, show the function keys and select Menu (→ page 64). From the Main menu, you can open the following menus: • Control On the Control menu, you can select the wavelengths for the measurement (→ page 69). • Preferences On the Preferences menu, select the settings for data acquisition and for the lamps (→ page 70).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.4.2.4 Preferences Menu On the Preferences menu, select the settings for data acquisition and for the lamps. To … Select … Set the data rate for the measurement.Select an option: Slow—0.5 Hz Medium—1 Hz Fast—2 Hz Max.—as fast as possible User— allows you enter the data step under Multi wvl. data step. User is indicated also when the detector is connected in Chromeleon.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.4.2.5 Diagnostics Menu The Diagnostics menu provides information for diagnostic purposes (read-only). In addition, you can perform a self test and other tests. To … Select … Perform a check (for example, filter validation or grating validation) and make the required settings. Checks See status information for various components and perform the related commands if necessary.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors To … Select … Turn on the selected lamp whenever the detector is powered up or when operation is resumed after standby mode has been cancelled.To turn the lamps on or off manually, use the lamp commands on the Preferences menu (→ page 70). Lamp power on defaults Set the display and function key parameters: Data display mode—determines for which data the related information appears on the screen.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.5 Information for Operating the Detector This section provides information that should be considered for operating the detector. To learn more about ... See page ...
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.5.1.1 Wavelength When you select a wavelength, make sure that the mobile phase is "transparent", showing little or no absorption at the selected wavelength.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors If the wavelengths are selected such that, for example, only analyte 1 can be measured at wavelength 1 and only analyte 2 at wavelength 2, an unavailable chromatographic separation can be compensated optically. The multi-wavelength data acquisition can be used especially for method development and peak-purity analyses.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.5.1.2 Mobile Phases Mobile phase quality significantly affects detection limits and instrument performance. To ensure optimal performance of the detector, observe the following guidelines: • Prepare all mobile phases with HPLC-grade solvents, reagent-grade chemicals, and filter HPLC-grade water. • Degas all mobile phases before use and maintain them in a degassed state.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.5.1.3 Mobile Phase Delivery System The pumping system should deliver continuous flow while ensuring intermixing of the mobile phase (if gradient elution is used). Fluctuations in pump backpressure can cause baseline noise. If the noise is synchronized with the pump stroke, check your HPLC pump. The plastics present in some solvent delivery systems are not fully compatible with the solvents commonly used in chromatography.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.5.2 Setting the Wavelengths Set the wavelengths at which the analysis is performed in Chromeleon or on the detector display. Observe the general guidelines on pages 74 and 86. To set the wavelengths in Chromeleon On the Signals page in the detector Properties dialog box, verify that the signals you want to record were selected by the Enabled check box during the installation of the detector (→ page 46).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors To turn on the lamps manually • In Chromeleon Open the Commands dialog box for the detector. Set UV_Lamp to On, to turn on the deuterium lamp. Set Visible_Lamp to On, to turn on the tungsten lamp. • On the front panel display Show the function keys and select Menu, and then select Preferences. To turn on the deuterium lamp, set UV lamp to On. To turn on the tungsten lamp, set VIS lamp to On.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors To monitor the lamp intensity from the front panel display 1. Show the function keys and select Menu. 2. Select Configuration, and then select Ready Check. 3. Select UV low intensity limit and enter the low limit for the intensity of the deuterium lamp. Select VIS low intensity limit and enter the low limit for the intensity of the tungsten lamp. 4. On the Main menu, select Diagnostics, and then select Information. 5.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Monitoring the progress of data acquisition on the detector display You can monitor the progress of data acquisition on the display. The number of wavelengths and the data display mode (absorbance, ratio, channel, or raw) determine how the information is displayed. The standard display mode is Absorbance. However, you can also select a different display mode (→ page 82). Absorbance [mAU] @ Pos: 254nm 1.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.5.6 Setting the Display Mode You can set the display mode for watching the progress of data acquisition on the detector display in Chromeleon or on the front panel display. The standard mode is Absorbance (→ Fig. 25, page 81). To set the display mode in Chromeleon 1. Open the Commands dialog box for the detector. 2. Select DisplayMode, and then select a mode from the list (Absorbance, Channel, Ratio, or Raw).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.5.8 Recording the Temperature Signals On the Signals page, the Temp_Lamphouse and Temp_Ambient check boxes are selected by default when the detector is installed and configured in Chromeleon (→ page 46). Chromeleon then generates the appropriate channels for recording the ambient temperature and lamphouse temperature. The channels are then available in the Commands dialog box for the detector.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.6 Special Functions in Chromeleon This section provides a short overview of some special functions that Chromeleon supports for the detector. To learn more about ... See page ... Optimizing the detector performance See below.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Fig. 26: UV Options page in the program wizard 5.6.1.1 Data Collection Rate The data collection rate is the number of data points per second (Hz) that Chromeleon collects from the detector and stores as raw data. The number of data points per second generated by the detector electronics depends on the detector version. Data can be generated with a maximum collection rate of 100 Hz.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors • In multiple wavelength applications, baseline noise increases compared to single-channel data acquisition. This is especially the case of the data rate is set to the maximum or close to the maximum. To reduce noise, select a lower data collection rate.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.6.1.4 Baseline Behavior If wavelengths are switched during a single-channel or multi-channel data acquisition, the chromatogram may show baseline jumps. You can define how the baseline behaves in these cases. Select whether you want to automatically perform an autozero (Zero), to append the baseline to the previous signal (Append), or to let the baseline "jump" to the new value (Free).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.6.2 Recording Absorbance Spectra The detector allows you to record the absorbance spectra of substances that are used in an analysis, and thus, for example, to detect peak impurities, or to identify optimum wavelengths for method development.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.6.3 Predictive Performance Predictive Performance provides various functions for estimating the lifetime of consumables and for monitoring and recording service and (re)qualification information. Commands Dialog Box Open the Commands dialog box and enter the limits for the predictive performance parameters. For a complete list of available commands and parameters, see the Chromeleon Help.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Color Description Orange (Only for monitoring Qualification properties.) The value has reached the specified limit. However, a Grace Period has been specified during which the component may still be operated. Red The value has reached the specified limit or the specified grace period has expired. Replacement, servicing, or qualification of the detector is overdue. The detector can no longer be operated.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.6.5 Operational Qualification and Performance Qualification Operational Qualification and Performance Qualification allow you to check and document the performance of the HPLC system. All materials required for performing qualification and detailed instructions are available on request.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.7 Shutting Down the Detector Observe the following precautions before interrupting the operation or before shipping the detector: • Even during periods of detector inactivity, a flow cell (or dummy flow cell) must be installed. This is to prevent that dust particles cause damage to the detector optics.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors To create a standby or shutdown program Select one of the following alternatives: • Select and perform the operating commands and parameters from the Commands dialog box. • Create and run a corresponding program to automate the process (→ page 62). • Use the SmartShutdown Wizard to create and run the program (see below). To create the program with the SmartShutdown wizard 1.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5.8 Routine and Preventive Maintenance The detector is made of high-quality components and materials to minimize maintenance requirements. All surfaces are resistant to weak acids, alkali, and organic solvents. Nevertheless, immediately wipe up all liquids spilled onto the detector surface, by using lint-free cloth or paper. If surfaces are exposed for longer periods, these liquids can cause damage.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 6 Troubleshooting 6.1 Overview The following features help you to identify and eliminate the source for problems that may occur during the operation of the detector or UltiMate 3000 system. Status Screens The status indicators on the front panel provide a quick visual check of the operational status of the detector. They indicate whether the detector is turned on, connected in Chromeleon, and operating properly (→ page 23).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 6.2 Messages on the Detector Display Each time a fault or error occurs during the operation of the detector, the Status LED on the front panel door changes to red and a message appears on the detector display. In this case, the Prev, Next, and Clear keys appear on the navigation bar. To … Select … Return to the previous message. Prev Proceed to the next message. Next Remove a message from the display.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Message Remedial Action Bad flow cell position Verify that the flow cell is installed correctly (→ page 126). Consider installing a different flow cell. The flow cell may be dirty. Clean the flow cell (→ page 125). Command rejected – device busy The detector was not ready to execute the command. Repeat the command after waiting for a short time. Check the Ready state in Chromeleon.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Message Remedial Action Filter control calibration failed Calibration is possible only when the lamps are turned on. Verify that the lamps are turned on. Turn the lamps off and on again if necessary and retry. Check the flow cell and retry. Reinitialize the filter motor by performing the Reset command in Chromeleon. Consider replacing the lamps (→ page 119).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Message Remedial Action High dark signal drift (may impair linearity) There may be too much stray light. Verify that the flow cell is installed correctly and completely (→ page 126). Check the air humidity of the detector environment. The humidity might be too high. Let the detector warm up for about 1 hour with the lamp(s) turned on and try again. Perform a Dark Current Test (→ page 90).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Message Remedial Action Lamp intensity determination failed Determination of the lamp intensity failed. Determine the lamp intensity manually by performing the related command on the Diagnostics > Status & special functions menu (→ page 71). Perform the Reset command in Chromeleon to reset the detector to the initial conditions upon power-up. Consider replacing the lamps (→ page 119).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Message Remedial Action Reference signal too high There may be too much stray light. Verify that the flow cell is installed correctly and completely (→ page 126). Spectral line validation failed Check the flow cell for correct positioning (→ page 126). Perform wavelength calibration to check the Dα line manually (→ Tip in section 7.2, page 117). Switch on UV lamp first The deuterium lamp is turned off.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Message Remedial Action VIS lamp signal overload. Verify that the flow cell is installed correctly (→ page 126). Wavelength calibration data error. Check the flow cell and retry. Verify that at least one lamp is turned on. Turn the lamp(s) off and on again and retry. Reinitialize the grating motor by performing the Reset command in Chromeleon. Consider replacing the deuterium lamp (→ page 121). Wavelength calibration error.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors When the detector is operated with Chromeleon and if communication between Chromeleon and the detector cannot be established, related messages may appear in the Chromeleon Audit Trail. Message Remedial Action VWD-3x00@USB-1610103 Device not found on the USB. The USB connection between the detector and the Chromeleon server may be interrupted. Check the USB connection. The power supply to the detector may be interrupted.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 6.3 Chromeleon Diagnostics Messages If the detector fails a diagnostics test, perform the instructions given here and repeat the test. Follow the remedial actions in the described order and repeat the test after each action. If the detector still fails the test, contact Service. Calibration Test failed Before you run the Calibration Test, run a Dark Current Test and Intensity Test, if you have not yet done so.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Test Result Remedial Action Zero order offsets channel mismatch 1. Do not yet store the new calibration values. 2. If a lamp was replaced, verify that the new lamp is correctly installed and reinstall the lamp if necessary (→ page 119). 3. If both lamps were turned on during the test, turn off the tungsten lamp. 4. Verify that the flow cell is correctly installed and reinstall the flow cell if necessary (→ page 126). 5.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Grating Motor Test failed Test Result Remedial Action There may be a mechanical error (for example, "initialization position mismatch", "zero order position mismatch", or "reference position mismatch"). 1. Repeat the test. 2. Turn the detector off, wait for 30 seconds, and then turn it on again by pressing the power switch on the rear of the detector.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Test Result Remedial Action Holmium line around ### nm out of specified range Holmium filter tranmission low. Bad wavelength calibration or holmium filter impaired. (where ### indicates the position) The first message appears if, in the measurement channel, the indicated wavelength deviates more than 2 nm from the reference value. If this error occurs, the transmission is checked, too.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Intensity Test failed In the test result, ### is replaced by "measurement channel" or "reference channel". Also note the shape of the intensity spectrum, as absorbing substances in the flow cell may cause local intensity drops. Measurement and reference intensity spectra must be qualitatively identical.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 6.4 Operating Problems The following table provides information about common operating problems that might occur with an UltiMate 3000 system and lists probable causes, as well as remedial actions. For more information, also see the manuals for the other modules of the UltiMate 3000 system.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Problem Probable Cause Remedial Action The system has very high backpressure. Fluidic parts in the system (capillaries, filter, column) are blocked by precipitate, or capillaries are damaged by bending. Check the capillaries in the system step by step from the detector to the pump, remove the blockage, or replace the capillaries. High baseline drift The shipping locks for the optics have not been loosened.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Problem Probable Cause Remedial Action High baseline drift (Cont'd) The gradient has different absorptions for the selected wavelength. Absorbing additives may change the absorption spectrum, depending on the solvent. The drift that is caused can be leveled by varying additive concentrations. Strong noise, non-periodic baseline fluctuation The eluent is dirty or their purity is insufficient. Use fresh eluent.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Problem Probable Cause Remedial Action Peak Broadening, increased dead time (Cont'd) The proportioning valve is defective. Contact Service. The column is overloaded or contaminated. Clean or replace the column. The eluent has changed. Use fresh solvent. Reproducible ghost peaks in the chromatogram. The degassing channels are contaminated. Rinse the degassing channels (→ Solvent Rack or Pump manual).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Problem Probable Cause Remedial Action Poor peak area precision The autosampler draws air from the vial. There is not enough amount of sample in the vial, the needle height setting is incorrect (→ Autosampler manual), or there are too many replicates. There are air bubbles in the syringe or the autosampler fluidics. Flush the syringe (→ Autosampler manual). Non-degassed wash solution is used.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Problem Probable Cause Remedial Action Poor peak area precision (Cont'd) The gradient is irreproducible. Change the gradient. Check the pump function and degassing. Check the filter frits in the solvent supply line filters for contamination. Replace the frits as necessary. The sample is unstable and decomposes. Use new sample or change the conditions. Cool the sample in the autosampler.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7 Service 7.1 General Notes and Safety Precautions The following sections describe all procedures for the detector that the user may perform. All other maintenance and service procedures must be performed only by Thermo Fisher Scientific service personnel. Warning: The fluid components of the device may be filled with solvents that are harmful to health. Wear appropriate personal protective equipment.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors If the original shipping container is not available, appropriate shipping containers and packing material can be ordered from Thermo Fisher Scientific sales organization for Dionex HPLC products. The packing instructions are included in the "Installation and Qualification Documents for Chromatography Instruments" binder and are available on request. For instructions on shutting down the detector, see page 92.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7.2 Wavelength Calibration and Verification Wavelength calibration is performed automatically • Each time when a detector lamp is turned on. • When two lamps are on and one of them is turned off. Calibration starts shortly after the lamp is ignited. The 0-order radiation, which corresponds to 000 nm of the wavelength scale, is checked.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors To perform wavelength verification in Chromeleon 1. Open the Commands dialog box for the detector. 2. Select WavelengthVerTest to start the verification. To see the test result, select WavelengthVerTestResult. The test result appears also in the Audit Trail. 3. If wavelength verification fails, perform wavelength calibration manually: a) Verify that the deuterium lamp is turned on.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7.3 Lamp Observe the following precautions before installing or exchanging a lamp. Safety Guidelines Warning: The deuterium lamp emits UV radiation that is harmful to the eyes and skin. The deuterium lamp can emit UV radiation from the rear side of the lamp (side of the connection wires) when it is installed in the detector. Therefore, avoid looking directly into the light source.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Tips: You can monitor the number of hours the lamp has been in operation: ♦ In Chromeleon In the Commands dialog box for the detector, select UVLampOperationTime (or VISLampOperationTime), and then select Value to see the total operating hours of the lamp.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7.3.1 Replacing the Deuterium Lamp Description Part No. Deuterium lamp 6074.1110 1. Observe the safety precautions on page 119 before you start the replacement procedure. 2. Loosen the screw that holds the lamp house cover in place by a quarter of a turn and then remove the cover and set it aside. Lamp house cover screw Fig. 27: Lamp house cover 3.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 4. Loosen the two screws in the lamp flange and pull out the lamp. Loosen these locks. Fig. 29: Deuterium lamp screws 5. Inspect the new lamp for fingerprints and dust. If necessary, clean the lamp with isopropanol. 6. Align the flange of the new lamp with the locating pin, and then gently push the lamp into the lamp housing.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7.3.2 Installing or Replacing the Tungsten Lamp Description Part No. Tungsten lamp 6074.2000 1. Observe the safety precautions on page 119 before you start the replacement procedure. 2. Loosen the screw that holds the lamp house cover in place by a quarter of a turn and then remove the cover and set it aside. Lamp house cover screw Fig. 31: Lamp house cover 3.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors When replacing the tungsten lamp Loosen the two screws in the lamp flange (→ Fig. 33) and pull out the lamp. Loosen these two screws. Loosen these two screws. Fig. 33: Tungsten lamp screws 5. Align the (new) lamp with the locating pin, and then gently push the lamp into the lamp housing. Be careful not to tilt the lamp and be sure that the lamp flange is in a level position with the lamp housing.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7.4 Flow Cell 7.4.1 Dummy Flow Cell The detector is shipped with a dummy flow cell (part no. 6074.0190). Before initial operation of the detector, replace the dummy cell with a flow cell (→ page 126). Tip: Keep the dummy flow cell. The cell is required for some diagnostics tests in Chromeleon (→ page 90).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7.4.3 Replacing the Flow Cell Safety Precautions Warning: When the flow cell is removed and the lamps are turned on, light emits in the flow cell opening from the opening on the left of the flow cell. The UV radiation is harmful to the eyes and skin. To avoid possible damage to the eyes and skin, turn off the detector before replaing the flow cell or wear UV glasses and appropriate protective clothing.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors • Tubing connections between the adapter block and the flow cell are installed at the factory and should not be opened by the user. • Capillary connections between the column and flow cell should be as short as possible to avoid peak broadening effects due to excessive dead volume. The following flow cells are available for the detector: * Part No. Description 6074.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7.4.3.1 Removing a Flow Cell (or Dummy Cell) without Adapter Block 1. Disconnect the capillaries from the cell inlet and outlet connection ports if applicable. 2. Loosen the two screws that hold the flow cell in position and pull the flow cell out of the detector. Flow cell screws Fig. 35: Flow cell (here dummy flow cell) 7.4.3.2 Removing a Flow Cell with Adapter Block 1.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7.4.3.3 Installing a Flow Cell (or Dummy Cell) without Adapter Block Tip: The contacts for the flow cell identification chip are located on the rear of the flow cell. To ensure optimum performance of the chip, be careful not to touch the contacts. 1. Push the flow cell into the flow cell holder and tighten the flow cell screws hand-tight. 2. Connect the capillaries shipped with the flow cell to the cell if applicable. 3.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 5. Remove the dummy plugs on the adapter block in and out connection ports. Connect the capillaries and route them to the outside through the slots provided in the detector enclosure. If you connect a stainless steel inlet capillary and if you want to route the capillary through the slot above the flow cell, Thermo Fisher Scientific recommends guiding the capillary through the slot as shown in figure Fig. 38. Fig.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7.5 Drying the Leak Sensor The leak sensor is installed inside the detector and reports a leak when liquid collects in the drip tray under the fluid connections. Eliminate the cause for the leakage and dry the leak sensor. 1. Turn off the detector. 2. Inspect the flow cell for signs of leakage. If the cell is leaking, tighten the connections to the flow cell. Consider replacing the flow cell (→ page 126). 3.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7.6 Replacing the Main Power Fuses Warning: Turn off the main power switch. Disconnect the power cord from its source. Avertissement: Avant de remplacer les fusibles, arrêtez le détecteur. Assurez-vous de bien débrancher le cordon d'alimentation de la source secteur. 1. Remove the fuse cartridge, by using a small screwdriver. Fuse cartridge Fig. 41: Fuse cartridge 2. Replace the fuses. Warning: Always install two new fuses.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 7.7 Updating the Detector Firmware The detector is shipped with the most recent firmware version. The detector firmware is also included in Chromeleon. To check which firmware version is installed in the detector and which version is included in Chromeleon: • Firmware version installed in the detector - Turn on the detector by pressing the power switch on the rear of the detector.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 3. Right-click the detector in the timebase and select Properties on the menu. 4. On the General page (→ page 44), the firmware version provided by Chromeleon for the detector is displayed in the Firmware field. If more than one firmware version is available for the detector in Chromeleon, select the version from the Firmware list. 5. Click Download.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 8 Technical Information Optical design: Dual-beam photometer (monochromator) Multiple wavelength UV/VIS detector Light source: VWD-3400RS: Deuterium lamp and tungsten lamp VWD-3100: Deuterium lamp; tungsten lamp available as an option Temperature control for both lamps Wavelength range: 190 to 900 nm The tungsten lamp is recommended for wavelengths > 600 nm. Noise: Single wavelength: <± 2.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Data collection rate: Single wavelength: Adjustable VWD-3100: up to 100 Hz VWD-3400RS: up to 100 Hz (Chromeleon 6.80) or 200 Hz (Chromeleon 7.1 or later) Multiple wavelengths: Adjustable, up to max. 5 Hz, depending on the number of wavelengths (2-4) and the distance between the wavelengths (in nm) Wavelength accuracy: ± 1 nm (over detector lifetime) Wavelength repeatability: ± 0.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Environmental conditions: Range of use: Indoor use Temperature: (50 °F to 95 °F) Air humidity: 80% relative humidity, condensing Overvoltage category: II Pollution degree: 2 Dimensions (h × w × d): 16 × 42 × 51 cm (6.3 × 16.5 × 20 in.) Weight: Approx. 14 kg (approx. 31 lbs) 10 °C to 35°C non- Technical Information: September 2013 All technical specifications are subject to change without notice.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Page 138 Operating Instructions
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 9 Accessories, Consumables, and Spare Parts Accessories, spare parts, and consumables for the detector are always maintained at the latest technical standard. Therefore, part numbers are subject to alteration. However, updated parts will always be compatible with the parts they replace. 9.1 Standard Accessories The following standard accessories are shipped with the detector.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 9.2 Optional Accessories Description Part No. Analytical flow cell (cell volume: 11 µL, cell material: PEEK, path length: 10 mm, pressure limit: 5 MPa) 6074.0200 Analytical flow cell (cell volume: 11 µL, cell material: stainless steel, path length: 10 mm, pressure limit: 12 MPa) 6074.0250 Semi-micro flow cell (cell volume: 2.5 µL, cell material: PEEK, path length: 7 mm, pressure limit: 5 MPa) 6074.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors * Description Part No. Remarks Capillary kit, Viper, for UltiMate 3000 Standard (SD) system with HGP pumps 6040.2309 Includes 3 capillaries with 0.18 mm ID for connections in an HPLC SD system with HPG-3x00SD pump. Capillary kit, Viper, for UltiMate 3000 XRS system with LPG-3400XRS pump 6043.2301 Includes 3 Viper capillaries (2 capillaries, one each of SST, 0.1 x 350 mm and 0.13 x 550 mm ID x L, and 1 capillary, PEEK, 0.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 9.3 Consumables and Spare Parts The part number always refers to the packing unit. Unless otherwise stated, the packing unit is 1 unit. For more information, contact the Thermo Fisher Scientific sales organization for Dionex HPLC Products. Description Part No. Capillary (PEEK, 1/16" x 0.25 mm O.D. x I.D., 1 m) 6251.6001 Capillary (PEEK, 1/16" x 0.50 mm O.D. x I.D., 1 m) 2251.6002 Capillary from TCC to VWD (0.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Description Part No. Flow cell, analytical (cell volume: 11 µL, cell material: PEEK, path length: 10 mm, pressure limit: 5 MPa) 6074.0200 Flow cell, analytical (cell volume: 11 µL, cell material: stainless steel, path length: 10 mm, pressure limit: 12 MPa) 6074.0250 Flow cell, capillary (cell volume, 45 nL, cell material: fused silica, path length: 10 mm*, pressure limit: 20 MPa) 6074.0280 Flow cell, dummy cell 6074.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors * Description Part No. Tungsten lamp 6074.2000 USB cable type A to type B, 1m 6035.9035 USB cable type A to type B, 5 m 6911.0002 UV monitor, ultra-low dispersion (cell volume: 45 nL, cell material: fused silica, path length: 10 mm*, pressure limit: 30 MPa) Recommended for the use in an UltiMate 3000 XRS system in combination with a mass spectrometer. For information about the UV monitor, refer to section 2.8 (→ page 28).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 10 Appendix 10.1 Common Mobile Phases The mobile phase composition affects its UV cutoff, that is, the minimum effective wavelength for the measurement. In general, mobile phases are solvents, such as, water, acetonitrile, methanol, or other substances. They may also contain salts, such as NaOH. The UV cutoff wavelengths of these solvents may differ from those predicted by Table 1.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Functional Group Chromophore Wavelength (nm) Aldehyde –CHO 280-300 Amine –NH2 195 Anthracene 252 375 Azido >C=N– 190 Azo –N=N– 285-400 Benzene 202 255 Bromide –Br 208 Carboxyl –COOH 200-210 Diphenyl 246 Disulfide –S–S– 194 255 Esters –COOR 205 Ether –O– 185 Iodide –I 260 Isoquinoline Ketone 218 266 317 >C=O Naphthalene 270-280 220 275 312 Nitrate –ONO2 270 (shoulder) Nitrite –ONO 220
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 10.2 Digital I/O The two digital I/O ports provide four TTL inputs and four relay outputs that can be used to exchange digital signals with external devices. Digital out relay 1 Digital out relay common Digital out relay 2 Digital out relay common Digital in plus 1 Digital in common Digital in plus 2 Digital in common Digital in plus 3 Digital out relay 3 Digital in plus 4 Digital out rely 4 Port A Port B Fig.
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 10.3 Declaration of Conformity for Holmium Oxide Filters Declaration of Conformity for Holmium Oxide Glass Filter The holmium oxide glass filters that are used in the Thermo Scientific Dionex detectors listed in the table below meet the requirements of the National Institute of Standards and Technology (NIST).
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors 11 Index A Absorbance Spectrum ........................................ 88 Accessories Optional ...................................................... 140 Standard ...................................................... 139 Analog out ......................................................... 71 Analog Output ............................................. 25, 38 Configure ......................................................
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors F L Firmware Download .................................. 45, 133 Firmware Update ....................................... 45, 133 Firmware Version ............................................ 134 Flow cell .......................................................... 125 Flow Cell ........................................................... 71 Clean ........................................................... 125 General ..............
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors PCM-3000.......................................................... 26 Performance Optimization ................................. 84 Performance Qualification ................................. 91 pH and Conductivity Monitor ............................ 26 Power Connection .............................................. 37 Power Switch ..................................................... 24 Power-Up ..................................
UltiMate 3000 Series: VWD-3100 and VWD-3400RS Variable Wavelength Detectors Page 152 Operating Instructions
