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User Manual Quest 2 10 Manual Synthesizer part number 900 1 14 Rev 3 ARGONAUT TECHNOLOGIES Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
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Table of Contents Introduction System Overview Operating the Quest 2 10 Maintenance Troubleshooting (To be supplied) Appendix A. Glossary of Terms Appendix B. Control Box Display Screens Appendix C. Quest Plumbing Schematic ARGONAUT TECHNOLOGIES Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
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Introduction This manual is intended to assist you with operating and maintaining the Quest 210 Manual Synthesizer. This manual is organized into the following chapters: Chapter 1, Introduction, contains general information on the Quest 210 Manual Synthesizer, warranties, and a brief description of the instrument. Chapter 2, System Overview, provides a definition of the Quest 210 hardware parts and an overview of how these parts work together.
Introduction Quest 210 Specifications Quest 210 Specifications REACTION VESSELS 1-20 disposable with frit 5 or 10 mL transparent teflon AGITATION Proprietary vertical oscillation mechanism -40°C to 130°C (requires recirculating chiller for temperature control below ambient) RACK COLLECTION Accommodates 20 and 40 mL scintillation vials, 13 x 100 rnrn, 20 x 125 mm, SPE cartridges DIMENSIONS Reactor Assembly (W x d x h) Weight 75 lbs Controller Unit (w x d x h) Weight 10" (25 cm) x 16.
Symbols Symbols The following hazard symbols are found on the Quest 210 Instrument: When heating, the Reactor Assembly will become hot. The symbol at the right is to warn of hot surface. Use caution when working near or on the Reactor Assembly. Allow it to cool before attempting to access RVs. This symbol indicates a shock hazard. Use care when working around an area with this symbol. QUEST 210 User Manual Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.
Introduction User Supplied ltems - Quest 210 Manual Synthesizer User Supplied ltems - Quest 210 Manual Synthesizer 30 - 40 psi house nitrogen supply or other inert gas. 110 VAC line supply for the Quest Controller Unit (The Quest will also operate on 90-240 VAC without any modification required.) User supplied chiller. Solvents and Reagents Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
Quest 210 Limited Warranty Agreement Quest 210 Limited Warranty Agreement HOW LONG IS THE WARRANTY (TERM) This Warranty Agreement begins on the invoice date of the Quest 210 and terminates either 3 months or 1 year from that date, depending on the type of warranty purchased. WHO IS PROTECTED This warranty may only be enforced only by the original purchaser. LIMITED WARRANTY (WHAT IS COVERED) 1.
Introduction Quest 210 Limited Warranty Agreement EXCLUSIONS (WHAT IS NOT COVERED) This warranty covers normal use and is limited in scope to loss or damage to the warranted product. Argonaut does not warrant and will not be held responsible for loss or damages resulting from a cause other than defects in material or worlunanshlp, including damage or loss caused by: 1.
SITE PREPARATION 4. Shipment boxes or packaging used to package systems and accessories which are returned to the factory for repair. It is the customer's responsibility to retain original system packaging. SITE PREPARATION It is the responsibility of the customer to prepare the site environmentally and provide for the required space and services such as power, venting, bottled and compressed gases, permits, licenses, approvals, etc.
Introduction lnterference Information lnterference Information This equipment generates and uses radio frequency energy and, if not installed and used in strict accordance with the manufacturer's instructions, may cause interference to radio and television reception. It has been type tested and found to comply with the limits for a Class B computing device in accordance with the requirements set forth in the European Standard EN 5501 1:1997.
Safety Safety This equipment has been tested and found to comply with the requirements as set forth in the European Immunity Standard: EN5OO82- 111997. The following hazard symbols are found on the Quest 210 Instrument: When heating, the Reactor Assembly will become hot. The symbol at the right is to warn of hot surface. Use caution when working near or on the Reactor Assembly. Allow it to cool before attempting to access RVs. This symbol indicates a shock hazard.
Introduction Contact lnformation Contact Information In the United States: Argonaut Technologies 887 Industrial Road, Suite G San Carlos, CA 94070 PHONE: 650.598.1350 888.598.1350 (Toll Free U.S. Outside California) FAX: 650.598.1359 In Europe: Argonaut Technologies A.G. St. Jakobsstrasse 148 Postfach 43 4 132 Muttenz 2 Switzerland PHONE: 41.6 1.465.9898 FAX: 41.61.465.9899 In Japan: Argonaut Technologies K.K. MK Komachi Bldg. 4-2-1 Kojimachi Chiyoda-Ku Tokyo 102 Japan PHONE: 8 1.3.3234.
Quest 210 Technical Support Contact Information Quest 210 Technical Support Contact lnformation Quest 210 Repair Center and Technical Support Hotline PHONE: (650) 598- 1350 FAX: (650) 598- 1359 Quest Technical Support email: support@argotech.com. Use this email address as an option for communication of service and support questions or comments The Marketing Department at Argonaut Technologies should be contacted via e-mail at support@argotech.
Introduction Quest 210 Overview Quest 210 Overview WARNING The Quest 210 Manual Synthesizer is designed for use in chemical synthesis including potentially hazardous chemical reactions. The potential hazards of chemical synthesis include but are not limited to burns, explosions, and exposure to toxic chemicals and carcinogens. Always wear eye protection and suitable gloves. Operate the instrument in a fume hood.
Quest 210 Overview The solvent bottle assembly consists of a cap to interface to a 4 L solvent bottle. The Cap assembly will alternatively pressurize or vent the 4 L bottle. The Solvent Bottle assembly connects to the Quest Reactor Unit and contains solvent for parallel delivery to the RVs for resin washing and system cleaning. The umbilicus links the Control Unit to the Reactor Unit. Solvent, gas pressure, agitation and temperature control information are supplied/communicated through the umbilicus.
Introduction Quest Installation Procedure Quest Installation Procedure Installation of the Quest involves connecting the system to a gas source, and making some simple tubing connections. The process should take less than 1 hour.
Installation Procedure - Installation Procedure - Clear a space in a fume hood for the Quest. The space should be about 3 ft. wide by 2 ft. deep and be at the left edge of the fume hood. Also, clear a space on the lab bench adjacent to the fume hood for the controller. Carefully unpack the instrument and place the Quest Reaction Unit in the hood and the Quest Controller on the lab bench next to the hood. Remove any packaging material from the units.
Introduction Quest Installation Procedure Figure 1-2 - Quest Controller Unit Umbilicus Connections Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
installation Procedure - Figure 1-3 Quest Reactor Unit Port ~ons Poa Ins Ier Man1 Levers ain r Manifc QUEST210 User Manual Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
Introduction Quest Installation Procedure Connect the Umbilicus to the Quest Controller unit. Press the round male electrical connector labeled P2 on the umbilicus (this connection has large pins,) into the round female connector on the Controller Unit labeled "Heaters." Twist the housing of the connector to lock the cable into place. Likewise, connect the male electrical connector labeled P3 into the receptacle labeled "Signal.
Connect the Reactor Unit Manifold Tubes The Solvent Bottle Cap has 3 female ports oriented in a "Triangle." (see diagram) Figure 1-4 - Solvent Bottle Cap Female Ports pFq Solvent Connect the umbilicus tubes labeled "Btl. Press" and "Solvent" to the corresponding ports on the Solvent Bottle Cap. In the accessory kit, there will be a tube with a fitting on only one end, this tube connects to the vent port of the Solvent Bottle Cap.
Introduction Quest Installation Procedure The 3 female bulk head fittings for these tube connections are located near the manual valves towards the edge of the front face of the Quest Upper Reactor Assembly. The tubes will lead from these upper bulkhead fittings to a set of 3 female fittings located on the Quest Upper Manifold assembly. These latter fittings are located in a canted "triangle" configuration on the Quest Upper Manifold Assembly (see diagrams).
Connect the Reactor Unit Manifold Tubes Figure 1-7 Quest Upper Reactor Assembly Install the three tubes on one side of the Quest Reactor Unit: Screw in the bushing for one end of the tube labeled "Mem. Press." to thle upper red h~ulk head fitting on the upper Reactor Assembly labeled "Manifold." Screw the other end of the tube into the female threaded port on the Upper Manifold located at the top of the "triangle of ports (see diagram previous page).
Introduction Quest Installation Procedure Screw in the bushing for one end of the tube labeled "Man. Del." to the remaining red bulk head fitting located on the front face of the upper Reactor Assembly. This will be the lower port of a set of 2 mounted together. Screw the other end of the tube into the female threaded port on the Upper Manifold at the right of the "triangle of ports (see diagram)." Repeat the tubing installation for the other side of the Quest Reactor Unit.
Connect Power to the Unit Connect the male "Quick Connect" fitting to a long Tygon tube (found in the ship kit) which will lead to the laboratory gas supply. Fit the Tygon tube all the way onto the barb of the male Quick Connect fitting and tighten the locking ring. Connect the other end of the Tygon line to the laboratory gas supply. It is recommended that the Quest use a "House Nitrogen System" as a gas supply.
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System Overview The Quest 210 system consists of the reactor assembly, control box, solvent bottle assembly, and umbilicus. The reactor assembly contains 20 reaction vessels for parallel synthesis along with the components needed for heating, cooling, and agitation. The Reactor Unit also contains the Upper Manifold Control Valves. which control solvent and gas flow in the reactor assembly, allowing for functions such as solvent addition, vessel draining, and inert gas pressure. The control box.
System Overview Reactor Assembly Reactor Assembly The reactor assembly provides the ability to do the following: Run up to 20 liquid or solid phase reactions in parallel Heatkool the reaction solutions Mix the reaction-solutions. Control the solvent and gas flow in the reactor assembly, thereby performing manual solvent addition to the reaction vessels, draining of solution from the reaction vessels, and applying a metered gas flow over the reaction solution Collect reaction products.
Reaction Vessels Reaction Vessels Reaction Vessels Each side of the Reactor Unit contains a reaction vessel bank where 10 vessels can be mounted, for a total of 20 synthesis positions. The reaction vessels are clear Teflon tubes, and are disposable - intended for one time use. A frit is pre-installed in the bottom of each reaction vessel. The reaction vessels are available in either 5 or 10 rnL sizes.
System Overview Reactor Assembly Reaction Mixing NOTE Agitation parameters are set using the Controller Unit. For instructions, see Operating the Quest 210, Setting Agitation Parameters. Agitation The Quest agitation system consists of a pneumatically actuated Magnet Bar which vertically oscillates behind the two reaction vessel banks. Small Teflon encapsulated magnets are placed in the RV tubes.
Reaction Mixing NOTE The ability to control the solvent and gas flow in the reactor assembly allows one to solvent to the reaction vessels, drain waste from the reaction vessels, collect product. into vials, and provide a gas sweep over the reaction vessels. You may control the solvent and gas flow by means of the Upper Manifold Control Valves and the Metered Gas Valve.
System Overview Reactor Assembly Manifold Control Valves Each side of the reactor assembly contains two manifold control valves: Control Valve 1 (on left) and Control Valve 2 (on right). Figure 2-1 Quest Manifold Control Valves (Solvent Delivery Configuration) Drain Solvent 0 Metered Gas Closed Utility 1 Vent 0 Closed Utility 2 Control Valve 1 can allow either solvent or drain gas (30 psi) into the corresponding upper manifold common pathway.
Manual Solvent Additjon to the Reaction Vessels Product Collection- Utilizes the metered gas option to supply 10 psi gas to the upper manifold common path, pressurizing the upper RV head space for draining of product liquid into collection vials. Product collection is done at a lower pressure than draining to eliminate splashing as the samples are collected. Inert Gas Blanket- Provides a constant pressure to the RVs of 10 psi. Inert Gas Sweep- Provides a metered flow of 10 psi gas across the RVs.
System Overview Reactor Assembly Solvent Bottle Assembly The solvent bottle assembly consists of a 4L Solvent Bottle, plastic Bottle Safety Carrier, and a valved Teflon cap. The solvent bottle cap has three Teflon lines attached to it: One to supply pressure to the bottle, the second to allow venting of the bottle, and the third is a liquid line which leads to the Quest Reactor Unit. The pressure and delivery tubes protrude from a point within the umbilicus.
Reaction Vessel Waste Draining NOTE Change the solvent bottle frit once per week. When a flow path is created from the Solvent Bottle Assembly delivery line to a reaction manifold, the gas pressure forces the fluid through the tubing, via the Umbilicus, into the reaction vessels. Luer Ports The luer ports are found at the top of each Upper Manifold. Each port is a beveled hole with a corresponding plug. These ports are larger than a standard luer, but seal by the same mechanism.
System Overview Reactor Assembly Waste Tray and Container The Quest is equipped with a rectangular glass container for waste collection. The glass waste container is equipped with a plug valve which can be used to drain the tank to a ventilated waste drum. Also, a hose may be attached to the valve and the end placed in a larger waste container. Install the glass waste container under the Reactor Unit. This container can be removed for product collection. The glass waste container sits on the Waste Tray.
Controller Unit Controller Unit The Controller Unit contains an LCD screen, keypad, electronics and firmware to control the Quest. The Controller unit controls the following functions: Reaction temperature. Reaction temperature duration. Agitation parameters including agitation frequency and stroke duration. Connections for the system gas pressure and electrical power.
System Overview Controller Unit LCD Screen and Keypad The Controller Unit LCD screen and keypad allow you to set the reaction temperature, temperature duration, and agitation parameters. LCD Display The LCD display presents the various Quest programming screens. These screens allow you to change reaction temperature, temperature duration, and agitation parameters. Also, the screens will display status on the agitation and temperature settings.
LCD Screen and Keypad to specify the reaction vessel size (5 or 10 rnL). (Vessel size is important so the proper heating algorithm can be applied.) Monitor Temperature Screen- Displays the setpoint of the reaction vessel banks, the actual temperature, and whether the heaters have been turned on (The heaters must be turned on through the keypad, they do not automatically engage.) Other Functions Screen -Enables access to functions not available on the main screens.
System Overview Controller Unit STARTISTOP Button From the Set Temperature display screen, the STARTISTOP button will start or stop the heaters. After you have set the reaction bank temperature and temperature duration on the Set Temperature screen, press the STARTISTOP button to turn on the heater for the first bank. Move the cursor to the line for the second bank of reaction vessels. Repeat for the second reaction vessel bank.
Control Box Connections to the Reactor Assembly Control Box Connections to the Reactor Assembly The control box has connections for the Umbilicus for interface to the Reactor Unit. These ports are: Heater Power - Supplies power to the heaters within each reaction vessel bank. Signal - This cable carries thermocouple readings from the Reactor Unit to the Controller Unit. Agitation Gas - Two ports on the Controller unit supply HP gas to the pneumatic gas cylinder.
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Operating the Quest 210 This chapter provides step-by-step instructions for using the Quest 2 10 Manual Synthesizer to complete a liquid or solid phase chemistry reaction. These instructions include 1. Preparing the Quest for a Synthesis 2. Installing the Reaction Vessels on the Quest 210 3. Running Reactions on the Quest 210 Preparing the Quest for a Synthesis 1. The Quest Reactor Unit should be placed in a fume hood. 2.
Operating the Quest 210 Remove Old Reaction Vessels NOTE Always wear gloves and eye protection when working with the Quest. 1. Raise the Upper Manifold up off of the reaction vessels. 2. Move the Upper Manifold to a position at the top of the Quest Reactor Unit. 3. Using the red-handled pliers (removal tool), grasp the top of the reaction vessel, and "twist-up", using the reaction vessel holder as a fulcrum, to break the reaction vessel free of the clamping ring. (See figures 1,2, and 3).
Remove Old Reaction Vessels Figure 3-2 Using the reaction vessel mount as a fulcrum, twist the vessel to "break it" from the Lower Manifold male fitting Figure 3-3 The reaction vessel is free from the Lower Manifold male fitting QUEST210 User Manual Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
Operating the Quest 210 4. With your fingers, remove the vessel and discard in an appropriate waste container. The magnet can be removed from the "crimped" top of the reaction vessel, either by cutting the crimped part off with a tubing cutter, or by squeezing open the crimped area. Figure 3-4 Remove the reaction vessel from the holder Installing New Reaction Vessels NOTE The Quest 210 Reactor Assembly has a Side A and Side B. Each side contains a bank of 10 reaction vessels.
Installing New Reaction Vessels I Set Temperature - - - - A: 90C B: 60C RVSize 5mL 1:00 0.00 OFF OFF 5mL b. Press the Forward button to move the cursor to the RV size line. c. Press either one of the Parameter buttons to set either 5 ml , or 10 ml vessel sizes. 2. If the reaction is solid phase, weigh out and add the synthesis support, or add ArgoCaps, to the reaction vessels.
Operating the Quest 210 Figure 3-5 Reaction vessel and installation tool 7. Press down on the Insertion Tool to set the reaction vessel over the male fitting of the lower manifold. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
Installing New Reaction Vessels Figure 3-6 Press the vessel into place Figure 3-7 Proper installation of the reaction vessels 8. Repeat this procedure for each vessel. QUEST 210 User Manual Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
Operating the Quest 210 WARNING Always use frits in the reaction vessels, even if the reaction is solution phase. Frits prevent particulates from harming or clogging the Quest Reactor Unit Lower Drain Manifold. 9. Push in on the Upper Manifold handle buttons and slide the Upper Manifold onto the top of the reaction vessels. Use the Upper Manifold Levers, found on the side of the Quest Reactor Unit to press the Upper Manifold male fittings into the tops of the reaction vessels: a.
Installing New Reaction Vessels Waste Tray and Container The Quest is equipped with a rectangular glass container for waste collection. The glass waste container is equipped with a plug valve which can be used to drain the tank to a waste drum. Also, a hose may be attached to the valve and the end placed in a larger waste container. Install the glass waste container under the Reactor Unit. This container can be removed for product collection. Place the glass waste container the Waste Tray.
Operating the Quest 210 Starting a Reaction Starting a Reaction This section explains how to do the following: Setting the reaction temperature, temperature duration time, and agitation parameters. Closing the Upper Manifold Membrane Switch. Performing manual parallel solvent additions . Manually adding reagents or solvents to the reaction vessels through the luer ports.
Setting the Reaction Temperature, Time, and Agitation Setting the Reaction Temperature, Time, and Agitation You set the reaction temperature, time, and agitation parameters by changing the appropriate parameters on one of the control box display screens. For detailed information on the contents of each display screen and how to use the control box components to change the temperature, reaction time, and agitation parameters, see Appendix B: Control Box Display Screens.
Operating the Quest21 0 Starting a Reaction 2. Set the Upstroke parameter. The Upstroke parameter is the amount of time the agitation bar will be in the upper position (This number is necessarily less than the agitation frequency value) The % Upward parameter controls the upstroke in the same manner as the "Upstroke" parameter, but is expressed in terms of a percentage of the agitation frequency, rather than a time.
Setting the Reaction Temperature, Time, and Agitation 4. Set the Stroke Velocity. - If desired, modify the agitation speed by moving the control box Agitator Needle Valve counter clockwise to increase the speed of the agitator bar and clockwise to decrease the speed of the agitator bar. NOTE The agitator needle valve is set to closed during shipment. Turn it counter clockwise to start agitation. 5. To stop the mixing, press the keypad Agitator ONIOFF button.
Operating the Quest 210 Starting a Reaction Temperature Control - The allowable reaction temperature range on the Quest is -40 to +130. For a particular reaction, do not program a temperature set-point which exceeds the boiling point of the reaction solvent. Each side of the Quest may have a different set point. To achieve sub-ambient temperatures, an external recirculating chiller is required. When using the chiller, only connect it to one side of the Quest at a time.
Temperature Control - Enter the Temperature Set-Point On the keypad, press the "Mode" button until the Set Temperature screen is displayed. I Set Temperature - - - - A: 90C B: lOOC RVSize 5mL 1:00 0.00 1OmL OFF OFF Use the Forward and Back (right and left arrows) buttons to place the cursor over one of the digits of the setting in the temperature field. Note that there is a separate temperature field for Side A and B.
Operating the Quest 210 Starting a Reaction 9. Make sure that the vessel size selection in the Set Temperature screen matches the RV size that is being used for the synthesis. If not modify the selection in the screen. 10. The temperature set point can be changed from an existing set point, by using the Set Temperature screen. 11.
Temperature Control - b.Wrap teflon tape around the threads of both of the 1/16" elbow fittings exiting the cooling channel on both sides of the heater assembly. c.Using a 314" socket (either an open end wrench or crescent wrench may also be used), tighten the chiller accessory fitting assembly to the 1/16" elbow fitting exiting the heater assembly. d.Push on the 2 Y2" piece of 318" thick pipe insulation provided. e.Instal1 the the heater block guard (pin 101634) to the front of the heater assembly. f.
Operating the Quest 210 Starting a Reaction Upper Manifold Membrane Switch In general set the Upper Manifold Membrane Switch to "Closed when running a reaction. This switch, when in the closed position, supplies 30 psi gas to the outside of a membrane located with in the Upper Manifold.
Parallel Solvent Addition and Vessel Draining on the solvent line and a Teflon bottle seal is present in the cap. Wait a minute or so for the bottle to pressurize. 2. Toggle the "Upper Manifold Membrane" switch to "Open." 3. Initialize the solvent flow to the reaction vessels by setting the left Manifold Control Valve to Solvent, and the right valve to Vent: drain solvent metered gas closed vent 0 closed utility 2 utility 1 4.
Operating the Quest 210 Starting a Reaction drain solvent metered gas closed utility 1 vent 0 closed utility 2 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
Parallel Solvent Addition and Vessel Draining Draining the Reaction Vessels Reaction Vessel draining is typically done during solid phase synthesis during resin wash steps, or when a reaction step is complete. 1. Verify that the rectangular glass waste container is installed below the reactor assembly. 2. Toggle the "Upper Manifold Membrane" switch to "Open." 3. Set the Manifold Control Valves as follows: metered gas drain solvent closed vent 0 closed utility 2 utility 1 4.
Operating the Quest 210 Starting a Reaction Changing a Solvent Bottle Changing the 4L solvent bottle involves back-flushing the solvent delivery line, removing the bottle, and changing the end line filter. 1. Reaction Vessels need to be in place on at least 1 side of the Quest. 2. Toggle the "Upper Manifold Membrane" switch to "Open." 3. On the solvent bottle cap, turn the dial to the "Vent" position. Wait 1 minute for the bottle to vent before proceeding. 4.
Parallel Solvent Addition and Vessel Draining 6. Wait 5 seconds, then set the Manifold Control Valves to the following configuration. This will use the Metered Gas pressure to blow gas back through the Solvent Bottle liquid line. Turn up the flow rate on the Metered Gas line by turning the needle valve counter clockwise. Leave the valves in that configuration for 1 minute to completely empty the line. metered gas drain solvent 0 utility 1 closed vent 0 closed utility 2 7.
Operating the Quest 210 Starting a Reaction Adding Solvent or Reagents Through the Luer Ports Solvents, liquid reagents and solids can be added to the reaction vessels through the luer ports located at the top of the upper manifold. Typically, these reagents are added via syringe or pipette. 1. Prior to adding solvents or reagents through the luer ports, it is recommended that the system be vented, as it may be under pressure. To vent the vessels, rotate the Manifold Control Valves as shown below.
Providing a Gas Sweep over the Reaction Vessels 5. Replace the luer plug onto the solid entry port. 6. Place both Manifold Control Valves to "Closed." Providing a Gas Sweep over the Reaction Vessels It is possible to provide a low flow rate sweep of the reaction vessel upper head space with nitrogen (or system gas) during reagent addition, or while a reaction is in progress. This is accomplished by setting the right hand Manifold Control Valve to "Metered Gas, and the left hand valve to Utility 1.
Operating the Quest 210 Starting a Reaction To stop the gas flow set the Manifold Control Valves as follows: metered gas drain solvent closed vent utility 1 0 closed utility 2 Collection of Liquid Reaction Products When it is time to collect reaction products from the Quest, the waste container can be removed and an appropriate collection rack installed.
Collection of Liquid Reaction Products 1. Toggle the "Upper Manifold Membrane" switch to "Open." 2. Prior to product collection, it is recommended that the system be vented to relieve any residual pressure that can cause splashing during product collection. To vent the vessels, rotate the Manifold Control Valves as shown below. Slowly approach the "Vent" setting on the valve to vent the pressure slowly to avoiding "bumping" the solution.
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Maintenance This section explains how to clean the Quest System after a synthesis has been completed. Perform three solvent washes (solvent delivery, agitation, drain) on each Reaction Vessel Bank used for synthesis. Select a solvent that will dissolve any solids or other residues that were generated during the synthesis. Fill the vessels about 314 full. Mix for 1 minute prior to draining the solvent.
Maintenance appropriate solvent. Do not allow any particulates to enter the through holes on the lower manifold male reaction vessel fittings. 7. Empty the glass rectangular tank and rinse clean. Do not leave the tank full of waste liquid when the system is not in use. Examine the plastic plate located above the waste tank. Wipe clean of any residue. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
5 Troubleshooting To be provided QUEST 210 User Manual Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
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Glossary of Terms Agitation - The Quest agitation system consists of a pneumatically actuated Magnet Bar which vertically oscillates behind the two reaction vessel banks. Small Teflon encapsulated magnets are placed in the RV tubes. As the Magnet bar oscillates, the magnets in the reaction vessels vertically oscillate with it, causing mixing to occur. Agitation Needle Valve - Controls the speed at which the agitator bar will move.
Glossary of Terms Luer Ports - Found at the top of each Upper Manifold. Each port is a beveled hole with a corresponding plug. Solvents, reagents, and solids may be added through the luer ports. Magnet Bar - An aluminum assembly with cylindrical magnets mounted along its length. The magnet bar is oscillated vertically moving magnets in the reaction vessels causing mixing of the reaction solution.
Upper Manifold - The upper manifold is an aluminum and telfon block that contains two common pathways: one for parallel solvent delivery drain gas, the other for venting, and metered gas. Utility Ports - The utility ports provide the ability to attach external accessories to the Quest. There are two utility ports - Utility Port 1 and Utility Port 2. The port input lines are teed, with tubes going to the corresponding Manifold Control Valves on each side of the Quest.
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Appendix B: Control Box Display Screens The display is a LCD screen on the control box. Some display screens allow you to view reaction temperature, time, and agitation parameters. Other display screens, with the aid of the control box buttons, allow you to change the reaction temperature, time, and agitation frequency. This appendix lists the various display screens and explains how to use the control box buttons to select a display screen and change the parameters on each display screen.
Appendix B: Control Box Display Screens Figure 2-1 Quest Controller Box and LCD Display Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
Sign-on Screen Sign-on Screen Quest 210 Argonaut Technologies The Sign-on screen identifies this instrument as the Argonaut Technologies' Quest 210. Table 2-1 Sign-on Screen Control Button Functions Results in... Pressing this button... I Firmware Version screen. I PARAMETER STARTISTOP I NIA STARTISTOP Heaters screen appearing I StartsIStops agitation. QUEST 210 User Manual Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
Appendix B: Control Box Display Screens Firmware Version Screen Firmware Version Screen - - - - Quest 210 - - - Rev : 1.00 Date: D e c 1, 1997 The Firmware Version screen shows the Quest 210 revision and the revision date. Figure 2-1 Firmware Version Screen Control Button Functions ... Results in.,. Pressing this button MODE I Agitation Control screen appearing PARAMETER I NIA I STARTISTOP AGITATOR ON STARTISTOP Heaters screen appearing I Starts/Stops agitation.
Agitation Control Screen Agitation Control Screen MixEvery : UpStroke % Upward : : I-- 2.0 s e c 1.0 sec 50 % Not Agitating - - - The Agitation Control screen allows you to set the agitation parameters. The Agitation Control screen contains three parameters: M i x E v e r y s e c , UpStroke , and % Upward. The M i x E v e r y s e c parameter is the agitation frequency. That is, the elapsed time between consecutive upstrokes.
Appendix B: Control Box Display Screens Agitation Control Screen Notice that the agitation parameters that you set apply to both Side A and B. Table 2-2 Agitation Control Screen Control Button Functions Pressingthis button.., Results in,.. ' I Set Temperature screen appearing FORWARD/BACK selecting a parameter on the screen PARAMETER increasing or decreasing the value of the chosen parameter I STARTISTOP Heaters screen appearing I StartsIStops agitation.
Set Temperature Screen Set Temperature Screen I Set Temperature - - - A: B: RV's 90C 1:00 ' OFF lOOC 0.00 OFF A: 5 m L B: 10 mL The Set temperature screen allows you to set reaction temperatures, specify the duration for the heating step, start or stop the heating of the reaction vessels, and select the reaction vessel size. Table 2-3 Set Temperature Screen Control Button Functions Monitor Temperature screen.
Appendix 6: Control Box Display Screens Monitor Temperature Screen Monitor Temperature Screen A(0ff) B (Off) SET ACT The Monitor Temperature screen shows the temperature control status. This is done by displaying for Side A and B the temperature at which you set reactions, the actual temperature at which the reaction is, and whether the heaters are on or off.
Other Functions Screen Other Functions Screen I-1. 2. 3. OTHER FUNCTIONS P r o c e e d t o Main A d j u s t LCD Show Fmwr V e r s i o n The Other Functions screen allows you to select from a list of functions not available on the main screens. Five "other functions" are available, and are described below.
Appendix B: Control Box Display Screens Other Functions Screen Table 2-5 Other Functions Screen Control Button Functions Pressing this button.,. Results in... The screen choice (1-5) indicated by the cursor will be engaged. - - Moves the cursor up or down to select one of the 5 screen options. Moves the cursor up or down to select one of the 5 screen options. -- - STARTISTOP AGITATORON I STARTISTOP Heaters screen appears I StartsIStops agitation Artisan Technology Group - Quality Instrumentation .
Contrast Control Screen Contrast Control Screen . . . Set Contrast , - - - - - - - 3- . . The Contrast Control Screen allows you to adjust the contrast of the display screen. Table 2-6 Set Contrast Control Button Functions ... Pressing this button Resutts in... I Return to the Other Functions Screen.
Appendix B: Control Box Display Screens Self Test Screen Self Test Screen - - Self Test----100% A / D =OK T C = OK OK XFMR=OK HTR= OK OK v : --000000 00000000 The Selftest Screen appears when the Mode Button is pressed, after selecting option 4 (Perform Selftest) on the Other Functions Screen. This initiates a selftest. The Selftest procedure is normally executed in the factory to verify the basic hardware functions of the unit.
Self Test Screen Table 2-7 SelfTest Screen Button Functions Pressingthis button.,. Results in... I Return to the Other Functions Screen. STARTISTOP AGITATORON QUEST I STARTISTOP Heaters screen appears I Starts/Stops agitation 210 User Manual Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
Appendix B: Control Box Display Screens Restore Defaults Screen Restore Defaults Screen This screen appears momentarily, after the Mode button is pressed, when option 5 (Restore Defaults) is selected on the Other Functions Screen. This causes all user parameter settings (such as the agitation frequency parameters, LCD display contrast, etc. ) to be reset to their factory preset default values.
Start/Stop Heaters Screen StartEtop Heaters Screen Stop Start A Start B Both A and B A ( i s now O F F ) B ( i s now OFF) The Stop Heaters screen allows you to start or stop the heaters. The Stop Heater screen appears when you press the STARTISTOP control box button while a screen other than the Set Temperature screen is active. Use the Forward and Back buttons to select one of the three option. Then press the Mode button to activate the chosen option.
Appendix C - QUEST 2 10 Plumbing Schematic Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.
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