Specifications
bottom of the Lower Manifold. The silver buttons will snap out to verify that the
collection tray is locked into position.
5.
Ensure that each Teflon collection line is inside its own scintillation vial and that the
two vent lines are clear of any vials.
6.
Move the agitator up by pushing the Agitation
UP
soft key located to the right of the
Quest Controller Unit display.
7.
Vent the Upper Manifold of the Quest to prepare for the controlled draining of the
RV contents into the scintillation vials.
a.
Turn the Manifold Control Valves to "CLOSED and "VENT".
b.
Close the Metered Gas Needle Valve by turning the valve clockwise (to the right).
c.
Turn the Manifold Control Valves to "CLOSED" and "METERED GAS".
8.
Collect the contents of the RVs into the scintillation vials by doing the following,
a. Open Lower Manifold Drain Valve for the first RV.
b. Slowly turn the Metered Gas Needle Valve counterclockwise (to the left) until the
RV begins to empty. Adjust the Metered Gas pressure to control the speed at
which the RV empties. Drain the contents of the RV slowly to prevent the
product from splashing.
c. After the first RV has emptied proceed to empty the other RVs individually, one
at a time following steps
7
and
8.
D.
Collection of
THF
Cleavage Washes
1.
Using a syringe, deliver
3
mL THF to the RVs by doing the following:
a. Vent the Upper Manifold by turning the Manifold Control Valves to "CLOSED"
and "VENT".
b. Remove the Luer Plug from the first RV Luer Port (twist and pull) and insert the
syringe needle below the Teflon lines of the male Teflon RV fitting (below the
stream of
Nz) and dispense
3
mL THF. Take care not to score the interior
surfaces of the Luer Port with the needle, as marring the surface can affect the
sealing of the Luer Port. After delivery, replace the Luer Plug (twist and push)
and
add THF to the remaining
9
RVs.
c. Close the Upper Manifold by turning both Manifold Control Valves to
"CLOSED".
2.
Adjust the Agitation
Bar
by doing the following,
a. Raise the Agitation
Bar
up by pushing the Agitation UP soft key located to the
right of the Quest Controller Unit display.
b. Use the
7/64"
hex wrench supplied with the Quest to loosen the Agitation Stops.
Adjust the Agitation Stops on the Reactor Unit so the maximum magnet height is
2
cm below the solvent level in the RVs. Tighten the Agitation Stops so both
stops are at the same height.
Argonaut Technologies
Part Number
1022%
04/02/98
Quest
Tutorial
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