M498E 08.10.NF.
Introduction Thank you for purchasing a Nikon product. This instruction manual is written for users of Nikon Inverted Metallurgical Microscope ECLIPSE MA200. To ensure correct usage, read this manual carefully before operating the product. • No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon. • The contents of this manual are subject to change without notice.
Safety Precautions To ensure correct and safe operation, read this manual before using the product. Warning and Caution Symbols Used in This Manual Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct usage, read this manual carefully before using the product. Do not discard this manual and keep it handy for easy reference.
Safety Precautions WARNING 1. Intended product use The product should only be used for microscopic observation. Do not use this microscope for other purposes. Additionally, do not try to put a large sample on the stage if it is larger than the stage. 2. Do not disassemble Disassembling the microscope or the microscope system may result in electric shock or malfunctions. Damage or injury that may occur due to mishandling is unwarranted.
Safety Precautions 7. Heat from the light source The lamp and the lamphouse become extremely hot. To avoid burns, do not touch the lamphouse while the lamp is lit or for thirty minutes after it is turned off. Additionally, to avoid the risk of fire, do not place fabric, paper, or highly flammable volatile materials (such as gasoline, petroleum benzine, paint thinner, or alcohol) near the lamphouse while the lamp is lit or for about thirty minutes after it is turned off. 8.
Safety Precautions CAUTION 1. Handle with care This product is a precision optical instrument. Handle the microscope system with care to avoid shock on impact. In particular, objectives may loose accuracy when exposed to even a weak physical shock. 2. Do not wet the microscope If the product gets wet, a short circuit may cause malfunction or abnormal heating of the microscope.
Safety Precautions • Do not hold the focus knobs, eyepiece tube, lamphouse, stage, and so on, when carrying the microscope. They may come off and may cause serious injury or malfunction. • Be careful not to pinch your hands or fingers during transportation. 6. Cautions on assembling the microscope • Be careful not to pinch your fingers or hands during assembly. • Scratches or fingerprints on the optical components, including lenses or filters etc., will adversely affect the image.
Contents Introduction ....................................................................................................................................................................... 1 Safety Precautions............................................................................................................................................................ 2 Warning and Caution Symbols Used in This Manual ...............................................................................................
Contents 3.13.2 3.14 Using the λ Plate (MA2-λP)........................................................................................................................... 45 3.14.1 3.15 3.16 3.17 3.15.1 Inserting/removing the DIC prism from the optical path ................................................................ 46 3.15.2 Setting the DIC prism .................................................................................................................... 46 3.15.
Contents 4.3 4.4 4.5 4.6 Network Connection...................................................................................................................................... 73 4.3.1 Unit system capable of establishing network................................................................................. 73 4.3.2 Viewing user control options and status displays on a PC or the DS-L2 ....................................... 74 4.3.3 Registering information on the objective .....................
1 Part Name and Function Eyepiece Sample holder 10X, 12.5X, 15X (and 10X are equipped with the mask eyepiece.) Eyepiece tube (Figure illustrates the MA2-TI3.) The stage is equipped with the standard sample holder (with a sample clip). (Refer to 3.8.) Sample clip Binocular eyepiece may also be used. (Refer to 3.4, 3.5, and 3.6.) Stage (Figure illustrates the MA2-SR.) (Refer to 3.8.) Objectives (Refer to 3.9.) Nosepiece (Figure illustrates the MA2-NUI5.
Chapter 1 Part Name and Function POWER LED Displays ON/OFF for the power switch or the light-status for the illumination lamp. Additionally, the display status remains the same when an external PC is in use.
Chapter 1 Part Name and Function Supporting Pillar for Dia-Illuminator 100W/Supporting Arm for DS-L2 attaching part Optical path changeover lever (eyepiece tube/back port) (Refer to 3.23 and 3.24.) Attach the supporting pillar for dia-Illuminator 100W or the supporting arm for DS-L2. (Refer to 3.4.) Press: Eyepiece tube/back port =100/0 Tool storage Stores the supplied hexagonal wrench and two hexagonal screwdrivers.
Chapter 1 Part Name and Function Lamphouse connector [LAMP DC12V50W] Connects the cord on the epi illumination lamphouse (12V 50W Precentered Lamphouse). Internal/External brightness control changeover switch [EXTERNAL ON/OFF] RS232C connector [HGFIE] Connects the C-HGFIE HG Precentered Fiber Illuminator. (Refer to 3.2.2, 3.22.1, and 3.23.1.) ON: Adjusts the brightness on PC; RS232C connector [NCNT] OFF: Adjusts the brightness on the microscope.
2 Microscopy This chapter describes the procedure of each microscopy. See Table 2.1 for the items required for each microscopy. • Refer to “4. Assembly,” when the product has not been assembled yet. • For detailed information about operations of parts of the product, refer to “3. Operation Details.” • Notes on using an external light source: The procedures described on the episcopic illumination in 2.1 to 2.
Chapter 2 Table 2.1 Microscopy BF microscopy under the epi illumination Page Illuminator p.16 to p.18 Microscopy Items required for the microscopy Revolving nosepiece 5 revo., 6 revo., 7 revo. Objective Other items required • For EPI objectives with 5 revo., LU nosepiece adapter M32-25 required ⎯ • For 6 or 7 revo., BD objective unavailable DF microscopy under the epi illumination p.19 Simplified polarization microscopy under the epi illumination p.20 5 revo.
Chapter 2 2.1 Microscopy Bright-field Microscopy under the Episcopic Illumination Turn on the power to light up the episcopic illumination lamp. 1 Turn on the power switch. (See 3.1.1.) The power LED on the front is lit. 2 Be sure that the Internal/External brightness control changeover switch is set to OFF “internal mode.” (See 3.2.2.) 3 Turn the brightness control dial to light up the lamp. (See 3.2.1.
Chapter 2 Microscopy Set the microscope for the bright-field microscopy under the episcopic illumination. If the current unit is the one shown in the column at the right edge under “Other items required” in Table 2.1, pull out each item from the optical path. 1 Push in the optical path changeover lever for the eyepiece tube and select 100% for the binocular eyepiece. (See 3.4.1.) 2-4 2-1 2 Push in the eyepiece tube/back port changeover lever to select 100% for the eyepiece tube. (See 3.4.1.
Chapter 2 Microscopy Set the desired magnification and observe the sample. 5-1 1 Turn the revolving nosepiece to place the objective of a desired magnification into the optical path. (See 3.9.2.) 2 Turn the coarse/fine focus knobs and refocus on the sample. (See 3.7.) 5-6 3 Turn the brightness control dial to adjust the brightness of the episcopic illumination. (See 3.2.1.) 4 Turn the field diaphragm dial so that the field diaphragm image circumscribes the view-field. (See 3.11.
Chapter 2 2.2 Microscopy Dark-field Microscopy under the Episcopic Illumination Attach the items required for the dark-field microscopy under the episcopic illumination to the microscope. (See Table 2.1.) Focus on the sample with the bright-field microscopy under the episcopic illumination. (See Pages 16 and 17.) Set the microscope for the dark-field microscopy under the episcopic illumination. 3-1 1 Turn the revolving nosepiece to place the objective of a desired magnification into the optical path.
Chapter 2 2.3 Microscopy Polarization Microscopy under the Episcopic Illumination (simplified/sensitive color) Attach the items required for the polarization microscopy under the episcopic illumination to the microscope. (See Table 2.1.) Focus on the sample with the bright-field microscopy under the episcopic illumination. (See Pages 16 to 17.) Set the microscope for the polarization microscopy under the episcopic illumination.
Chapter 2 2.4 Microscopy Differential Interference Contrast Microscopy under the Episcopic Illumination Attach the items required for the differential interference contrast (DIC) microscopy under the episcopic illumination to the microscope. (See Table 2.1.) Focus on the sample with the bright-field microscopy under the episcopic illumination. (See Pages 16 and 17.) Set the microscope for the DIC microscopy under the episcopic illumination.
Chapter 2 Microscopy Return to the bright-field microscopy under the episcopic illumination. 1 Pull the polarizer/analyzer unit to the first stop-click position and remove the polarizer/analyzer from the optical path. (See 3.13.1.) 2 Both the λplate and the polarizer/analyzer unit are removed from the optical path. (See 3.14.1.) 3 Detach the DIC slider to remove the DIC prism from the optical path. (See 3.15.1 or 3.16.2.
Chapter 2 Microscopy Return to the bright-field or dark-field microscopy under the episcopic illumination. 1 Turn the BD field changeover lever to the “BF (bright-field)” or “DF (dark-field)” position. (See 3.3.) 2 Turn the brightness control dial to adjust the brightness. (See 3.2.1.) 3 Adjust the brightness with the desired ND filter in the filter turret. (See 3.10.
Chapter 2 2.6 Microscopy Bright-field Microscopy under the Diascopic Illumination Attach the items required for the bright-field microscopy under the diascopic illumination. (See Table 2.1.) Turn on the power to light up the diascopic illumination lamp. (See 3.23.1.) 1 Set the brightness control dial to OFF on the MA200 main body. (See 3.2.1.) 2 Turn on the TI-PS100W power supply connected to the supporting pillar for dia-Illuminator. 3 Set the MA200 main body to ON. The power LED on the front is lit.
Chapter 2 Microscopy Set the microscope for the bright-field microscopy under the diascopic illumination. When the “Other items required” (See the column at the right edge of Table 2.1.) is used for the episcopic illumination, remove each attachment from the optical path in advance. 1 Push in the optical path changeover lever for the eyepiece tube and select 100% for the binocular eyepiece. (See 3.4.1.) 2 Push in the BD field changeover lever to select the “BF (bright-field)” position. (See 3.3.
Chapter 2 Microscopy Place the sample onto the stage to adjust the focus. 1 Use the sample holder according to the selected sample. Place the sample onto the stage and move the stage using the stage movement knob for the X/Y direction so that the observation position comes to the center of the view-field. (See 3.8.) 4-2 4-1 2 Turn the coarse/fine focus knobs and focus on the sample. (See 3.7.) 4-1 Adjust the diopter and the interpupillary distance. 1 Adjust the interpupillary distance. (See 3.5.
Chapter 2 Microscopy Center the condenser. (See 3.23.2.) 1 Be sure that the objective is set to the 10X objective. If not, turn the revolving nosepiece to place the 10X objective into optical path. 7-2, 6 2 Stop down the field diaphragm by turning the field diaphragm dial on the supporting pillar for dia-Illuminator until the field diaphragm image comes into the view-field. 7-3 7-4, 7 3 Turn the condenser focus knob for the supporting pillar for dia-Illuminator to focus on the field diaphragm image.
Chapter 2 2.7 Microscopy Polarization Microscopy under the Diascopic Illumination (simplified/sensitive color) Attach the items required for the polarization microscopy under the diascopic illumination to the microscope. (See Table 2.1.) Focus on the sample with the bright-field microscopy under the diascopic illumination and center the condenser. (See pages 23 to 26.) Set the microscope for the polarization microscopy under the diascopic illumination.
3 3.1 3.1.1 Operation Details Power ON/OFF Power of the microscope The power switch for the product is located beside the AC inlet on the rear of the microscope. To turn on the product, set the power switch to the “|” side. To turn off the microscope, set the power switch to the “c” side. The power LED on the front lights up when the power is turned on. Power switch “|” side: ON “c” side: OFF POWER LED lights up Figure 3.
Chapter 3 3.2 3.2.1 Operation Details Illumination Brightness control and illumination ON/OFF Adjust the brightness of the lamp for the lamphouse (LV-LH50PC) with the brightness control dial on the left of the microscope. With the power switch remained on, turn the dial clockwise to increase the brightness or turn the dial counter-clockwise to decrease it. Turn the dial to OFF to turn off the lamp. Brightness range OFF position Index Brightness control dial Figure 3.
Chapter 3 3.2.3 Operation Details Displaying the POWER LED The status of the illumination lamp appears on the Power LED located on the front of the microscope. POWER switch OFF: LED lights off POWER switch ON, brightness control dial OFF: orange lights up POWER switch ON, brightness control dial ON: green lights up The display status remains the same when an external PC is in use.
Chapter 3 Table 3.3-1 Microscopy Revolving nosepiece Objective • For EPI objectives with 5 revo., LU nosepiece adapter M32-25 required BF microscopy under the epi illumination 5 revo., 6 revo., 7 revo. DF microscopy under the epi illumination 5 revo. only BD objective only Operation Details Selecting the microscopy method Items required for the optical BD changeover path lever Remarks BF (Press) Normal bright-field microscopy under the episcopic illumination.
Chapter 3 3.4 3.4.1 Operation Details Eyepiece Tube Selecting optical path • The light distribution for both the binocular and vertical tube parts of the MA2-TI3 trinocular eyepiece tube can be performed using the optical path changeover lever. Light distribution Lever position Binocular tube Vertical tube IN (Press) 100 0 OUT (Pull) 0 100 Optical path changeover lever Figure 3.
Chapter 3 3.4.2 Operation Details Adjusting the eyelevel risers Eyelevel risers can be used to adjust the height of the eyepiece tube for the user’s eye point. Up to two eyelevel risers can be attached in piles. When one eyelevel riser is attached, the eyepiece height rises 25 mm.
Chapter 3 3.6 Operation Details Adjusting the Diopters Adjust the diopter according to the user's eyesight using the diopter adjustment rings on the eyepieces. Diopter adjustment corrects the difference in the left and right eyesight. This adjustment facilitates binocular observation and minimizes focal deviation when switching objectives. Make sure to adjust the diopter adjustment rings on both eyepieces.
Chapter 3 3.7 3.7.1 Operation Details Adjusting the Focus (for focus operation) Using the coarse/fine focus knob Focus on the sample lifting/lowering the revolving nosepiece with the fine/coarse focus knob. The relationship between the direction of coarse/fine focus knob rotation and the revolving nosepiece vertical movement is shown in the figure. To raise the revolving nosepiece, turn the knob forward. • One revolution of the coarse focus knob drives the revolving nosepiece approximately 4.0 mm.
Chapter 3 3.8 3.8.1 Operation Details Placing the Sample and Operating the Stage Placing the sample Sample holder Sample Sample clip Normally, a sample can be placed on the stage with the sample holder. It can be also placed directly on the stage without the sample holder. In either case, be sure that the observation position comes to the center of the view-field. Figure 3.8-1 Placing the sample ■ Sample holder To use the sample holder, place it onto the stage.
Chapter 3 3.8.2 Operation Details Changing the Observation Position The rectangular stage is equipped with the knob to move the stage in all four directions. The upper knob is used for Y direction (back and forth), and the lower knob for X direction (left and right). The stage knob has a universal joint that allows you to move the stage knob direction being tilted at 28 degrees. This offers easy operation and user convenience for switching between the focus knob and the stage knob.
Chapter 3 3.9 3.9.1 Operation Details Operating the Revolving Nosepiece and the Objective Revolving nosepiece in combination with the objective For the MA200, you can use various revolving nosepieces including the quintuple, sextuple or septuple revolving nosepiece to attach the industrial CFI objective lens. The combination varies depending on the microscopy applied. Refer to “4.2 Combination List for the Unit” to select an appropriate combination.
Chapter 3 3.10 Operation Details Filter Two quartet filter turrets are provided on the microscope, and each filter turret has the φ25 mm filters as shown below. Filter currently in the optical path displayed Filter turret 1 Each time you turn the turret 90 degrees, the next filter enters the optical path. The display indicated on the turret is the filter placed in the optical path. Select the filter suitable for your purpose, and place it into the optical path by turning the turret.
Chapter 3 3.11 Operation Details Adjusting the Field Diaphragm (for the episcopic Illumination) Image of the field diaphragm The field diaphragm is used to limit the irradiation area of the lamp to the view-field of the microscope. The field diaphragm dial (F. S.) changes the opening of the field diaphragm. Normally, stop down the field diaphragm to a size that circumscribes (or inscribes) the view-field.
Chapter 3 3.12 Operation Details Adjusting the Aperture Diaphragm (for the episcopic illumination) Objective pupil Aperture diaphragm The aperture diaphragm controls the numerical aperture of the illumination system, and is closely related to the resolution of the optical image, the brightness, the contrast and the depth of focus. When the size of the aperture diaphragm is stopped down, the resolution and the brightness are reduced while the contrast and the depth of focus are increased.
Chapter 3 Operation Details ■ Adjustment with centering telescope (C-CT) Remove one of the eyepieces and attach the centering telescope instead. Turn the eyepiece of the centering telescope to adjust the focus. This will allow you to view the objective pupil (a bright circle) and the aperture diaphragm image. 3.13 Using the Polarizer/Analyzer Unit (MA2-PA/MA2-UPA) Second click-stop position First click-stop position Polarizer rotation ring Polarizer Analyzer Slot for λ plate Figure 3.
Chapter 3 3.13.2 Operation Details Adjusting the polarizer direction Each time you turn the polarizer rotation ring, the direction of the polarizer is changed. You can turn the polarizer 360 degrees. When the “+” mark on the polarizer rotation ring is aligned with the “T” mark, the crossed Nicol’s position is made. When the “=” mark on the rotation ring is aligned with the “T” mark, the open Nicol’s position is made.
Chapter 3 3.14 Operation Details Using the λ Plate (MA2-λP) First click-stop position Attach the λ plate to the polarizer/analyzer unit to perform sensitive color polarization microscopy. The λ plate cannot be used for the simplified polarization microscopy under the diascopic illumination. Second click-stop position λ plate Figure 3.14-1 3.14.1 λ plate slider Inserting/removing the λ plate Push the λ plate slider into the polarizer/analyzer unit placed in the optical path.
Chapter 3 3.15 Operation Details Using the DIC Slider (L-DIHC/L-DIC) Prism position index Prism position Sliding limit groove Prism setting dial Prism movement knob Figure 3.15-1 3.15.1 First click-stop position Attach the L-DIHC/L-DIC DIC slider to the quintuple nosepiece (MA2-NUI5 or LV-NU5A) in combination with the MA2-PA polarizer/analyzer unit to perform the DIC microscopy under the episcopic illumination in single NR method. The L-DIHC slider is designed for high contrast observation.
Chapter 3 3.15.3 Operation Details Interference color You can change the interference color continuously by turning the prism movement knob. Interference color Dark color Observations similar to the dark-field microscopy can be performed. Gray Phase contrast distribution of the whole sample with a bird’s eye view can be observed. Sensitive red-violet 3.16 Characteristics Observations with the highest color contrast can be performed. Using the DIC Slider (LV-DIHC/LV-DIC) DIC Slider Figure 3.
Chapter 3 3.16.3 Operation Details Interference color You can change the interference color continuously by adjusting the orientation of the polarizer. (Refer to 3.12.3.) Interference color Dark color Observations similar to the dark-field microscopy can be performed. Gray Phase contrast distribution of the whole sample with a bird’s eye view can be observed. Sensitive red-violet 3.17 Characteristics Observations with the highest color contrast can be performed.
Chapter 3 3.18 Operation Details Using the λ Plate (D-LP) λ plate First click-stop position Attach the D-LP λ plate to the sextuple revolving nosepiece (D-ND6) to perform sensitive color polarization microscopy under the diascopic illumination. The D-LP λ plate is not required to use the TI-DIC λ plate on the supporting pillar for dia-Illuminator 100W. Second click-stop position Figure 3.18-1 3.18.
Chapter 3 3.19 Operation Details Using the Fluorescent Unit (MA2-FL) Attach the fluorescent unit to the MA200 main body to perform the epi-fl microscopy. Second click-stop position First click-stop position A fluorescent unit consists of two types of optical components: an excitation light filter (EX filter), a barrier filter (BA filter). You can use the four types of combination, "G", "B", "BV" or "V" as shown in the table below. Select the fluorescent unit suitable for your purpose and sample.
Chapter 3 3.19.2 Operation Details Excitation light filter (EX filter) Spectral transmittance EX filter 0 Bandwidth Wavelength An excitation light filter transmits lights selectively and blocks other lights. The transmitted lights are called excitation lights. They are used to excite the fluorophore in the sample and fluorescent lights are emitted from the sample. The wavelength range of lights that can pass through the filter is called the bandwidth.
Chapter 3 3.19.3 Operation Details Barrier filter (BA filter) A barrier filter transmits only fluorescent lights emitted by the sample but blocks the excitation lights. This filter makes it possible to observe the fluorescent image without unnecessary light (that is, on a dark background).
Chapter 3 3.20 Operation Details Using the Scale Slider (MA2-GR/MA2-MR) Attach the MA2-GR/MA2-MR slider to the MA200 main body to measure steel structures or to photograph images. 3.20.1 Grain scale slider (MA2-GR) The following two types of scales are provided for a single slider for Austenitic steel measurement. • Austenitic steel measurement scale (ASTM E112-63 reticle numbers 1 to 8) Compare the reticle pattern (numbers 1 to 8) with the sample-structure size in order to measure the grain size.
Chapter 3 3.20.2 Operation Details Scale slider (MA2-MR) Three items of reticles including “magnification,” “length indication” by a line, and length indication by the unit of “xx μm” are provided for photographic images. The reticle is compatible with 5X, 10X, 20X, 40X, 50X and 100X objectives, and turn the dial of the slider to switch to the reticle which suits the magnification of the objective in the optical path.
Chapter 3 3.21 Operation Details Using the Intermediate Magnification Unit (MA2-MC) Attach the intermediate magnification unit to the MA200 main body to change the observation magnification for the binocular, vertical tube or the back port. Turn the turret for this unit to select the desired magnification from the magnifications 1X (dummy), 1.5X and 2X. Set the turret at the desired magnification displayed on the turret (click-stop). Magnification Magnification changeover turret Figure 3.
Chapter 3 3.22 Operation Details Using the HG Precentered Fiber Illuminator (C-HGFI/C-HGFIE) The specified light source LV-LH50PC may not provide sufficient brightness to perform epi-fl microscopy. In such case, use an HG precentered fiber illuminator that uses a high intensity mercury lamp as a light source. HG Precentered Fiber Illuminator Figure 3.22-1 HG Precentered Fiber Illuminator 3.22.
Chapter 3 3.23 Operation Details Using the supporting pillar for dia-Illuminator 100W (MA2-DP) Attach the supporting pillar for dia-Illuminator 100W to the MA200 main body to perform the microscopy under the diascopic illumination. To perform the microscopy, mount the D-LH/LC Lamphouse and condenser etc. to the supporting pillar for dia-Illuminator 100W, and connect the power supply (TI-PS 100W) to the lamphouse.
Chapter 3 3.23.2 Operation Details Focusing and centering the condenser To perform the diascopic illumination for the first time or to replace the current condenser, focus and center the condenser so that the light through the condenser is focused on the correct position of the sample surface (at the center of the optical path). 1 Be sure to focus the 10X objective on the sample as described in “2.6 Bright-field Microscopy under the Diascopic Illumination.
Chapter 3 3.23.3 Operation Details Adjusting the field diaphragm (for the diascopic illumination) The field diaphragm is used to limit the irradiation area of the lamp to the view-field of the microscope. View the supporting pillar for dia-Illuminator 100W from above, then turn the field diaphragm dial counter-clockwise to open the field diaphragm, or turn the field diaphragm dial clockwise to stop down the diaphragm.
Chapter 3 3.23.4 Operation Details Adjusting the aperture diaphragm (for the diascopic illumination) The aperture diaphragm controls the numerical aperture of the illumination system, and is closely related to the resolution of the optical image, the brightness, the contrast and the depth of focus. Aperture diaphragm lever Objective pupil plane Aperture diaphragm image Adjust the size of the aperture diaphragm image to 70-80% the size of the objective pupil plane. Figure 3.23-5 3.23.
Chapter 3 Operation Details Filter list Filter Description ND2 (Neutral Density filter) Adjusts the brightness for general microscopy or photomicroscopy. Reduces the light intensity to 1/2. Dims the quantity of light to 1/2 (Transmittance: approx. 50%). ND16 (Neutral Density filter) Adjusts the brightness for general microscopy or photomicroscopy. Dims the quantity of light to 1/16 (Transmittance: approx. 6.3%).
Chapter 3 3.23.8 Operation Details Polarizer slider (T-P2), λ plate (TI-DIC) When performing the simplified polarization microscopy under the diascopic illumination, mount the polarizer slider on the condenser mount for the supporting pillar for dia-Illuminator 100W. Push in the slider, and the polarizer enters the optical path. If you loosen the rotation clamp screw and slightly turn the polarizer, the image contrast changes.
Chapter 3 Mount rotation clamp screw Operation Details 7 Loosen the condenser mount rotation clamp screw. Hold the condenser turret and turn both the condenser and the polarizer at the same time. Tighten the mount rotation clamp screw to fix it at the position where a dark cross appears in the objective pupil. Be careful not to accidentally loosen the mount rotation clamp screw during microscopy. Dark cross that appears in the objective pupil Figure 3.
Chapter 3 3.24 Operation Details Using the Supporting Arm (MA2-MP) The supporting arm is designed to mount Nikon camera control unit DS-L2. The Supporting Arm for DS-L2 is equipped with a one-touch release. If you pull up the mount arm attaching/detaching knob, the DS-L2 can be attached or removed from the supporting arm.
Chapter 3 3.25 Operation Details Using the DS Camera Control Unit (DS-L2) Once you connect the DS Camera Control Unit DS-L2 with the DS camera attached to the vertical tube on MA200 or the back port using the specified cable, you can operate the DS camera. Additionally, by connecting MA200 with DS-L2 via USB cable, the following functions are available on the DS-L2.
Chapter 3 Operation Details ■ Nosepiece address Name of the objective NA value of the objective WD value of the objective Displaying the objective info Press the button, and the name and other info are displayed of the objective at the nosepiece address that is currently placed into the optical path (see Figure 3.25-2). Close the objective info area with the X button displayed on the right of the nosepiece address. Figure 3.
Chapter 3 3.25.5 Operation Details Connecting with the DS camera head switcher (DS-SW: Camera switch BOX, optional) If the DS-L2 is connected with the DS camera head switcher, two camera heads can be connected to the DS-L2. If the DS-L2 is connected with the DS-SW, the register tab appears on the DS-L2 microscope info menu. On the register tab, the “Measurement interlock for the magnification value of the microscope” function can be set to ON/OFF for each camera head.
4 Assembly WARNING WARNING and CAUTION at the • Before assembling the microscope, be sure to read the beginning of this instruction manual and follow the instructions written therein. • To prevent electrical shocks and fire, turn off the power switch (set the switch to the “c” side) and unplug the power cord from the outlet when assembling the microscope. CAUTION • Be careful not to pinch your fingers or hands during assembly.
Chapter 4 Assembly ■ Combination of the illuminator and the light source Use this product with a specified light source. The specified light source devices are as follows: For the episcopic illumination • Lamphouse Nikon 12V 50W Precentered Lamphouse (model name: LV-LH50PC) • Lamp Nikon 12V 50W longlife halogen lamp (model name: LV-HL50W), or 12V 50W shortlife halogen lamp from other manufacturers (model name: OSRAM HLX 64610, OSRAM HLX 64611, or PHILIPS 7027).
Chapter 4 4.
Chapter 4 4.2 Assembly Combination List for the Unit Combinations for each microscopy provided for MA200. : All selectable or available. c: Selections limited. Only the unit under this symbol is available. /: Unselectable or unavailable. n: Notes on combinations. Table 4.
Chapter 4 Assembly 1: For EPI objective on 5 revo., “LU nosepiece adapter M32-25” is required. 2: BD objective is unavailable on 6 revo., 7 revo. 3: The CFI L Plan EPI objective lens cannot be used for DIC microscopy under the epi-illumination, epi-fl microscopy. 4: D-DA analyzer is attachable for 6 revo. only. 5: D-LP λ plate is attachable for 6 revo. only. 6: L-DIC/L-DIHC slider is attachable for 5 revo. only. 7: LV-DIC/LV-DIHC slider is attachable for 6 revo. only.
Chapter 4 4.3 Assembly Network Connection 4.3.1 Unit system capable of establishing network The table below shows a unit system capable of establishing network on a PC or the DS-L2 connected to MA200 via the USB cable.
Chapter 4 4.3.2 Assembly Viewing user control options and status displays on a PC or the DS-L2 The table below shows user control options and status displays of a unit that can be controlled on a PC or the DS-L2 connected to MA200 via the USB cable. The “/” indicates external control/status display is unavailable. To control MA200 on a PC or to display the current status of the MA200 on a PC, use the “SDK” in the supplied “MA200 Setup Tool” software.
Chapter 4 Table 4.
Chapter 4 4.3.3 Assembly Registering information on the objective You can register the information to be checked on a PC or the DS-L2 with the “MA200 Setup” in the supplied “MA200 Setup Tool” software. For details on how to install the “MA200 Setup” on an appropriate device, refer to the software manual supplied with “MA200 Setup Tool.” 4.3.
Chapter 4 4.4 Assembly The LV-LH50PC Lamphouse and the Lamp CAUTION for heat Do not touch the lamp and the lamphouse while the lamp is on or for thirty minutes after it has been turned off. CAUTION • To prevent electrical shock and damage to the microscope, always turn off the power switch (set the switch to the “O” side) and unplug the power cord from the outlet before attaching or detaching the lamphouse or replacing the lamp.
Chapter 4 4.4.2 Assembly Replacing the lamp 1 Loosen the lamphouse cover clamp screw using the supplied hexagonal wrench. 2 Remove the lamphouse cover. (1) 3 Press the lamp clamp levers and remove the old lamp. (3) (2) 4 With the lamp clamp levers pressed, insert the electrodes of a new lamp into the holes of the socket. Insert the lamp to the limit, and release the lamp clamp levers to secure the lamp. Be careful not to touch the glass surface of the lamp with bare hands.
Chapter 4 4.5.2 Assembly Cable connection for the manual revolving nosepiece and the MA200 Connect the cable of the revolving nosepiece to the connector on the microscope. 1 Loosen the rear-panel clamp screw on the microscope with the supplied hexagonal screwdriver to remove the rear-panel. 2 Connect the cable of the revolving nosepiece to the connector on the microscope. Connect the cable of the MA2-NUI5 revolving nosepiece to the round connector on the microscope.
Chapter 4 4.6 Assembly Stage • To attach or remove the stage, hold the stage securely to prevent from being fallen. • To remove the stage from the microscope with the supporting pillar for dia-Illuminator 100W mounted, be sure that the stage does not come into contact with the condenser, etc. • A cross roller guide is provided to move the stage in the X/Y direction. The Y direction movement rack is located at the bottom of the stage. Do not touch the rack during your operation, as you may get injured.
Chapter 4 4.7 Assembly Objectives 1 Remove the sample holder from the stage. 2 Screw the objectives into the socket of the revolving nosepiece securely through the stage hole. Attach the objectives so that the magnification increases when the revolving nosepieces are turned clockwise as viewed from above.
Chapter 4 4.10 Assembly Grain Scale Slider/Scale Slider 1 Remove the dust protection cover from the scale slider slot on the bottom-front of the microscope. Be sure to face the indication on the slider to the correct direction. Insert the slider in the direction shown in the figure. 2 Securely insert the slider to the limit. • When removing the scale slider, do not touch or press hard against the glass. It may cause damage to the micrometer reticle.
Chapter 4 4.12 Assembly Various Sliders for Attaching the Revolving Nosepiece To insert a slider, be sure to attach the objective in advance. 4.12.1 L-DIC/L-DIHC slider (single NR method) For using the DIC slider in single NR method, attach the MA2-NUI5 revolving nosepiece or LV-NU5A motorized nosepiece. Sliding limit groove 1 Loosen the DIC slider limit screw on the nosepiece using a hexagonal screwdriver. 2 Remove the slot from the revolving nosepiece to insert the DIC slider.
Chapter 4 4.12.2 Assembly LV-DIC/LV-DIHC slider (Senarmont method) Attach the DIC slider in Senarmont method to the D-ND6 revolving nosepiece. DIC slider slot 1 Select the slider (A or B) corresponding to the objective to be used (refer to 3.16) 2 Insert the slider into the slot corresponding to the position of the objective. Push the slider into the limit to place the DIC prism in the optical path. 3 If you do not want to use the DIC slider, remove it from the revolving nosepiece.
Chapter 4 4.13 Assembly Polarizer/analyzer Unit and Fluorescent Unit 1 Loosen the front-cover fixing bolt with the supplied hexagonal screwdriver to remove it. Turn to loosen the BD field changeover lever counter-clockwise, then remove the lever and front-cover from the main body. Fluorescent unit Polarizer/analyzer unit λ plate 2 Insert the unit into the slot inside the microscope. Push the unit into the second click-stop position, the polarizer/analyzer or fl filter enters the optical path.
Chapter 4 4.14 Assembly Intermediate Magnification Unit 1 Remove four bolts to take out the cover on the attaching part for intermediate magnification unit with the supplied hexagonal screwdriver. 2 The cables are secured by the cable keeper inside the opening as the figure shows. Remove the cable from the cable keeper, then connect the cable to the connector on the intermediate magnification unit. * Cable Cable keeper The cable keeper is attached to MA200 with a double-side tape.
Chapter 4 4.15 Assembly External Light Source You can attach the HG precentered fiber illuminator (for the episcopic illumination) or the supporting pillar for dia-Illuminator 100W as an external light source. CAUTION • To use an external light source, carefully read the instruction manual and make sure to follow the instructions. • To assemble and connect parts, check that the power supplies for the light source and the product are turned off and that the power cord is unplugged from the outlet. 4.
Chapter 4 Assembly * 4.15.2 For the C-HGFIE motorized fiber illuminator, connect the C-HGFIE and the [HGFIE] connector on the MA200 using the RS232C cross cable. Attaching the MA2-DP supporting pillar for dia-Illuminator 100W When working, hold the dia pillar illuminator to prevent it from falling.
Chapter 4 Assembly Attach the condenser to the condenser mount. You can attach the TI-C condenser turret (system condenser) or ELWD-S condenser to the condenser mount. Additionally, attach the TI-C-LWD condenser lens or the MC TMD2 ELWD condenser lens to the system condenser.
Chapter 4 ■ Assembly Attaching the ELWD-S condenser With the indication on the turret facing the front (towards the user), insert the condenser turret into the bottom of the condenser holder, and secure it by tightening the condenser clamp screw. Dovetail Condenser indication position Condenser clamp screw ELWD-S condenser Attach the D-LH/LC Lamphouse to the supporting pillar for dia-Illuminator 100W. Insert hexagonal screwdriver and tighten clamp screw.
Chapter 4 Assembly Attach/replace the lamp. Be sure to use the halogen lamp (OSRAM HLX64623 or PHILIPS77241) exclusive for the D-LH/LC Lamphouse. CAUTION for heat Do not touch the lamp and the lamphouse while the lamp is on or for thirty minutes after it has been turned off. WARNING • When replacing the lamp, turn off the power supply and unplug the power cord. • The lamp and its surroundings will be hot while the lamp is on and immediately after it is turned off.
Chapter 4 Assembly Attach the filter and the slider. • Do not touch filters or other optical components with your bare hands. • Latches at both ends of the filter slider are at their end point when sliding. When removing a filter slider, you can slide it out by pushing up the latch on the opposite side of the tab with your finger to release the filter slider. Applying undue force on the filter slider can break the latches. 1 Attach the desired filter to the filter slider.
Chapter 4 Assembly Attach the T-P2 polarizer slider. Fixing ring 1 Remove the system condenser, if attached, from the condenser holder to attach the T-P2 polarizer slider. Pin 2 Mount the polarizer slider on the condenser mount, and align the positioning pin for the polarizer slider with the groove on the condenser mount. Turn the fixing ring to secure the polarizer slider.
Chapter 4 4.16 Assembly Supporting Arm (for DS-L2) You can mount the camera control unit DS-L2 on the MA200 main body by attaching the supporting arm. 1 Loosen clamp screws (2) using the supplied hexagonal screwdriver and remove the cover from the attaching part of the supporting pillar for dia-Illuminator 100W/supporting arm on top-surface of the microscope. 2 Align the pins (2) on the lower-surface of the supporting arm with the holes on the attaching part.
Chapter 4 Assembly ■ Attaching the DS-L2 1 Remove the one-touch release attached to the front of the mount arm for the supporting arm. Mount arm attaching/detaching knob One-touch release Pull up the mount arm attaching/detaching knob, then hold up the docking section forward to remove it. Rotation torque nuts (x2) 2 Detach the stand arm attached on the rear of the DS-L2 by removing the four clamp screws.
Chapter 4 4.17 Assembly Eyelevel Risers Eyepiece tube clamp screw Eyepiece tube clamp screw 1 Fully loosen the eyepiece tube clamp screw on the microscope, to mount the eyelevel riser onto the attaching part for the eyepiece tube, then fitting the dovetail on the eyelevel riser to the circular groove of the attaching part. 2 Secure the eyelevel riser by tightening the eyepiece tube clamp screw. 3 Attach the eyepiece tube on the eyelevel riser and secure the eyepiece tube with clamp screw.
Chapter 4 4.18 Assembly Camera Adapter You can perform photomicroscopy by mounting the photomicrographic equipment such as CCD camera on the microscope with the LV-TV TV or the C-mount adapter attached. Apply the camera adapter to the vertical tube port on the MA2-TI3 trinocular eyepiece or the back port on the upper-surface of the microscope. Be sure to apply the adapter or zoom lens compatible with the camera. Clamp screw Camera 1 Remove the cap from the vertical tube port/back port.
5 Troubleshooting Improper use of the microscope may adversely affect performance, even if the microscope is not damaged. If any of the problems listed in the table below arise, take the countermeasures indicated. If the problem is not listed below, or if the problem cannot be resolved by the suggested countermeasure, unplug the power cord and contact your nearest Nikon representative. 5.1 Viewing Problems and Control Problems Problem The view-field is invisible, vignetted, or uneven in brightness.
Chapter 5 Problem Dirt or dust is seen in the view-field. Troubleshooting Cause Countermeasure The aperture diaphragm is stopped down too far. Open the diaphragm to a suitable size. Dirt or dust exists on the intermediate magnification unit. Clean the components. (p.102) Dirt or dust exists on the lens, eyepiece, filter or sample. Clean the components. (p.102) Dirt or dust exists on the scale slider or grain scale slider. Clean the components. (p.
Chapter 5 Problem The brightness is insufficient. (Refer to the troubleshooting for the electric system too.) Troubleshooting Cause Countermeasure The lamp voltage is too low. Adjust the brightness with the brightness control dial. (p.30) An ND filter is placed in the optical path. Remove the ND filter from the optical path. (p.40) The aperture diaphragm is stopped down too far. Open the diaphragm to a suitable size.
Chapter 5 Problem Troubleshooting Cause Uneven colors are seen or low contrast image is seen in the DIC microscopy. Countermeasure A wrong objective is used. Place an appropriate objective into the optical path. (p.32) The orientation of the polarizer is wrong. Adjust the orientation of the polarizer correctly. (p.44) No sensitive color is seen in the DIC microscopy or the polarization microscopy. 5.2 Dust exists on the objective, the condenser or the sample. Clean it.
6 Care and Maintenance Nikon recommends daily care and maintenance for maintaining the performance as long as possible. Do not let dust, fingerprint, etc., get on the lenses. Dirt on the lenses, filters, and the like will adversely affect the optical performance of the microscope. If lenses are contaminated, clean them according to the procedure described in “6.1 Cleaning the Lenses and Filters.” When cleaning, be sure to turn off the power switch (set the switch to “c” side) to avoid any operation error.
Chapter 6 6.3 Care and Maintenance Storage • Store this product in a dry place where mold is not likely to form. • Store the objectives and eyepieces in a desiccator or similar container with a drying agent. • Put the dust-proof cover over this product to protect it from dust. • Before putting on the dust-proof cover, turn off the power switch of the product (set the switch to the “Ο” side) and wait until the lamphouse gets cool sufficiently. 6.
7 Specifications Type ECLIPSE MA200 Dimension 413 (W) × 337 (D) × 308 (H) mm, including the handle and mount parts Weight 20 kg Optical system Objective: CFI60 system (chromatic aberration free infinity optics system) Eyepiece: Field number: 22, 25 Revo. nosepiece: 5 revo. (MA2-NUI5, LV-NU5A) 6 revo. (D-ND6) 7 revo.
Chapter 7 Power cord Specifications When used in 100-120 V region, outside Japan: UL listed detachable power cord set, 3 conductor grounding (3 conductor grounding Type SVT, No.
