Instruction manual

Chapter 3 Operation Details
58
3.23.2 Focusing and centering the condenser
To perform the diascopic illumination for the first time or to replace the current condenser, focus and center
the condenser so that the light through the condenser is focused on the correct position of the sample surface
(at the center of the optical path).
Move the field diaphragm image into the center of the
view-field. Adjust its size to match the view-field.
Figure 3.23-3 Focusing and centering
the condenser
1 Be sure to focus the 10X objective on the sample
as described in “2.6 Bright-field Microscopy under
the Diascopic Illumination.”
2 Stop down the field diaphragm by turning the field
diaphragm dial on the supporting pillar for
dia-Illuminator 100W until the field diaphragm
image comes into the view-field.
3 Turn the condenser focus knob of the supporting
pillar for dia-Illuminator to focus on the field
diaphragm image.
4 Turn the condenser centering screws (2 for both
sides) for the supporting pillar for dia-Illuminator to
move the field diaphragm image to the center of
the view-field.
5 Place the 50X objective into the optical path.
6 Adjust the size of the field diaphragm image to
closely match the size of the view-field, by turning
the field diaphragm dial on the supporting pillar for
dia-Illuminator.
7 Turn the condenser centering screws on the
supporting pillar for dia-Illuminator to move the
field diaphragm image to the center of the
view-field.
* To observe the sample, turn the field diaphragm
dial so that the field diaphragm image
circumscribes the view-field. (Perform this
adjustment for each objective.)
Field
diaphragm
dial
Condense
r
centering
screws
Condense
r
focus knob
Condenser
focus knob
Field diaphragm image
Vie
w
-field