J326XPI-IM-4 Avanti® J-26 XPI High-Performance Centrifuge Instruction Manual
Symbol Symbol Symbole Símbolo Simbolo Title / Titel / Titre / Titulo / Titolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione ! Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung) Marche (mise sous tension) Encendido Acceso (sotto tensione) O
! SAFETY NOTICE This safety notice summarizes information basic to the safe operation of the equipment described in this manual. The international symbol displayed above is a reminder that all safety instructions should be read and understood before installation, operation, maintenance, or repair of this instrument. When you see the symbol on other pages, pay special attention to the safety information presented.
Mechanical Safety For safe operation of the equipment, observe the following: • Use only the Beckman Coulter rotors and accessories designed for use in this centrifuge. • Do not exceed the maximum rated speed of the rotor in use. • NEVER attempt to slow or stop a rotor by hand. • Do not lift or move the centrifuge while a rotor is spinning. • NEVER attempt to override the door interlock system while the rotor is spinning. • Do not lean on the centrifuge or place items on it while it is operating.
J326XPI-IM-4 July 2009 Avanti® J-26 XPI High-Performance Centrifuge Instruction Manual MADE © 2009 Beckman Coulter, Inc. IN U.S.A.
Contents Page INTRODUCTION Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii Scope of this Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii Notes, Cautions, and Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . viii Typographic Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . .
Contents Page SECTION 2: DESCRIPTION Centrifuge Function and Safety Features . . . . . . . . . . . . . . . . . . . . . . . 2-1 Centrifuge Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1 Safety Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2 Housing and Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3 Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Contents Page Programmed Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14 Creating a New Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14 Modifying a Program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16 Running a Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-18 Setting Up a Zonal Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Illustrations Page Figure 1-1. Single-Phase Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . 1-10 Figure 1-2. Three-Phase “Y” Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . 1-10 Figure 1-3. Correct and Incorrect Three-Phase Power Configurations . . . . . . . . . . 1-11 Figure 1-4. Rear View and Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-12 Figure 1-5. Checking the Rotor for Drive Pins . . . . . . . . . . . . . . . .
Introduction CERTIFICATION To ensure full system quality, Beckman Coulter Avanti® J-26XPI centrifuges are manufactured in a registered ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies. Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com.
Introduction • The Appendix contains a procedure to use when temperature control within ±1°C is required. ➠ NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and performance of this equipment could be impaired. Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual is the sole responsibility of the user.
Introduction ! WARNING Used whenever an action or condition may potentially cause personal injury or loss of life. Mechanical damage may also result. WARNING Indicates high voltage or risk of electric shock. Refer servicing of all areas displaying either symbol to qualified service personnel. TYPOGRAPHIC CONVENTIONS Certain typographic conventions are used throughout this manual to distinguish names of user interface components, such as keys and displays.
Introduction CANADIAN REGULATIONS This digital apparatus does not exceed the Class A limits for radio noise emissions from digital apparatus as set out in the radio interference regulations of the Canadian Department of Communications. Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage radioelectrique édicté par le Ministère des Communications du Canada.
1 Specifications and Preinstallation Requirements SPECIFICATIONS Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. CONTROL FEATURES CFC Speed Setting range. . . . . . . . . . . . 100 to 26 000 rpm (in 10-rpm increments) or equivalent RCF (in 100 × g increments) Elutriation speed setting range . . . . . . . . . . . 0 to 5 000 rpm (in 10-rpm or 10 × g increments) Speed display . . . . . . . . . . . . . . . . . . . .
Specifications and Preinstallation Requirements Deceleration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . maximum, slow, timed (1 to 10 min from 500 to 0 rpm), or off OPERATIONAL FEATURES Door. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1-cm (2.4-in.) thick structural foam with steel plate Rotor chamber diameter . . . . . . . . . . . . . . . . . . . . . . . . . . . 51.3 cm (20 in.) Friction Reduction System (FRS) . . . . . . . . . . . . . . . . . 190 mm (7.5 in.
Specifications and Preinstallation Requirements AVAILABLE ROTORS Refer to the applicable rotor manual for complete rotor descriptions. Rotor Code Max RPM a Max RCF (× g) Max Capacity Rotor Manual Number rmax = 108 mm 30.50 26 000 c 81 800 8 × 50 mL J-TB-070 rmax = 108 mm 25.50 25 000 75 600 8 × 50 mL J-TB-056 rmax = 106 mm (outer row) 25.15 25 000 74 200 24 × 15 mL J-TB-057 Rotor Profile and Description JA-30.50 Ti Fixed Angle, 34° (8 place) b JA-25.
Specifications and Preinstallation Requirements Rotor Code Max RPM a Max RCF (× g) Max Capacity Rotor Manual Number rmax = 108 mm 20 20 000 48 400 8 × 50 mL J-TB-003 45° adapter rmax = 116 mm 18.1 18 000 42 100 24 × 1.8 mL J-TB-037 17 000 36 300 24 × 1.8 mL Rotor Profile and Description JA-20 Fixed Angle, 34° (8 place) JA-18.
Specifications and Preinstallation Requirements Rotor Code Max RPM a Max RCF (× g) Max Capacity Rotor Manual Number rmax = 137 mm 14 14 000 30 100 6 × 250 mL J-TB-004 rmax = 153 mm F50C 14 000 33 500 14 × 50 mL — rmax = 137 mm F250 14 000 30 000 6 × 250 mL — rmax = 144 mm 12 12 000 23 200 12 × 50 mL J-TB-051 rmax = 158 mm 10 10 000 17 700 6 × 500 mL J-TB-006 rmax = 166 mm 10.
Specifications and Preinstallation Requirements Rotor Code Max RPM a Max RCF (× g) Max Capacity Rotor Manual Number rmax = 158 mm F500 (previously F10) 10 000 17 696 6 × 500 mL — rmax = 185 mm 9.1000 9 000 16 800 4 × 1000 mL J-TB-073 rmax = 222.8 mm 8.1000 8 000 15 900 6 × 1000 mL J-TB-073 rmax = 140 mm 13.1 13 000 26 500 6 × 50 mL J-TB-036 rmax = 161 mm 24.38 10 000 e 18 000 6 × 38.5 mL J-TB-058 rmax = 171.3 mm 24.
Specifications and Preinstallation Requirements Rotor Code Max RPM a Max RCF (× g) Max Capacity Rotor Manual Number rmax = 165 mm 7.5 7 500 10 400 4 × 50 mL J-TB-007 rmax = 194.8 mm 5.3 5 300 6 130 24 microplates 8 deep-well plates 4 square-well plates J-TB-089 rmax = 204 mm 4.3 4 300 4 220 4 × 750 mL J-TB-050 rmax = 226 mm 4.0 4 000 4 050 4 × 1000 mL J6-TB-006 JCFZ 20 000 660 mL (standard core) JCFZ-IM Rotor Profile and Description JS-7.5 Swinging Bucket (4 place) JS-5.
Specifications and Preinstallation Requirements PREINSTALLATION REQUIREMENTS Do not attempt to install this instrument. Its purchase price includes installation by Beckman Coulter personnel. Installation by anyone other than an authorized Beckman Coulter representative invalidates any warranty covering the instrument. Preinstallation requirements have been sent prior to shipment of the instrument. The following information is provided in case the centrifuge must be relocated.
Specifications and Preinstallation Requirements Table 1-1.
Specifications and Preinstallation Requirements 30-ampere Circuit Breaker Wall Outlet: Hubbell 9330, Bryant 96-30-FR, or Equivalent (NEMA 6-30 R) Measured Line Voltage EarthGround 30-ampere Circuit Breaker Figure 1-1. Single-Phase Electrical Requirements 16-ampere Circuit Breaker Measured Line Voltage Phase to Phase Neutral 16-ampere Circuit Breaker EarthGround 16-ampere Circuit Breaker Figure 1-2. Three-Phase “Y” Electrical Requirements Table 1-2.
Specifications and Preinstallation Requirements Additional Requirements for Three-phase Power Connections For three-phase power service to the centrifuge, use the “Y” connected configuration shown in Figure 1-3. Also note the following information: • The steady state current draw of the centrifuge can be as high as 12 amperes per phase, depending on the voltage. • Inrush current to the centrifuge is 100 amperes for up to 2 seconds during the refrigeration start period.
Specifications and Preinstallation Requirements • In addition to space for the centrifuge, allow a 7.7-cm (3-in.) clearance on each side of the centrifuge and a 16-cm (6.25-in.) clearance behind the centrifuge for air circulation. The centrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during centrifuge operation. • Position the centrifuge so that the air diverter, shown in Figure 1-4, touches the wall behind the centrifuge.
Specifications and Preinstallation Requirements ! WARNING Do not place the centrifuge near areas containing flammable reagents or combustible fluids. Vapors from these materials could enter the instrument’s air system and be ignited. SECURING THE CENTRIFUGE TO THE FLOOR Avanti J series centrifuges are certified to meet the requirements of the European CE mark. To meet these requirements, the centrifuge must be secured to the floor using the anchoring hardware shipped with the instrument.
Specifications and Preinstallation Requirements USING J2 SERIES ROTORS IN THE AVANTI J ! CAUTION Do not use the Beckman Coulter JA-10, JS-7.5, JA-14, or JS-13 rotors in the Avanti J-26XPI centrifuge before reading this information. CHECKING FOR ROTOR DRIVE PINS Rotors used in Avanti J series centrifuges must have drive pins in the rotor drive hole (see Figure 1-5). These drive pins engage with the centrifuge drive spindle hub to ensure that the rotor does not slip during acceleration.
Specifications and Preinstallation Requirements B E CKMAN Rotor Drive Hole Drive Pins (Angled pins shown; pins can also be vertical or horizontal.) KM EC A N B Drive Spindle Assembly Top View Side View Figure 1-5. Checking the Rotor for Drive Pins USING THE JCF-Z CONTINUOUS FLOW/ZONAL ROTOR Zonal Bracket Kits A special bracket and mounting hardware are required when the JCF-Z Continuous Flow/Zonal Rotor is used. These parts are contained in the Zonal Bracket Kit (part number 366431).
Specifications and Preinstallation Requirements See REPLACEMENT PARTS AND SUPPLIES in Section 5 for a complete list of Zonal Bracket Kit part numbers and kit contents. Ensuring Correct JCF-Z Rotor Identification The centrifuge’s rotor identification system can, under two specific conditions, misidentify the JCF-Z continuous flow/zonal rotor. These conditions and corrective actions are listed below.
Specifications and Preinstallation Requirements the lubrication, perform three runs from 0 to 5000 rpm and back to 0 rpm. ➠ NOTE If the JCF-Z rotor is misidentified, the run speed will be limited to the maximum speed for the identified rotor. (The maximum speed for the JCF-Z rotor is 20 000 rpm.
2 Description This section describes the Avanti J-26 XPI centrifuge components and their functions. It also describes system safety features and centrifuge controls and displays. Refer to the applicable rotor manual for rotor descriptions. CENTRIFUGE FUNCTION AND SAFETY FEATURES CENTRIFUGE FUNCTION The Avanti J-26XPI is a refrigerated centrifuge that generates centrifugal forces required for a wide variety of applications.
Description The Avanti J-26XPI centrifuge is microprocessor-controlled, providing interactive operation.2 The instrument design features a brushless switched-reluctance drive motor,3 automatic rotor identification system, FRS (friction reduction system) vacuum control circuitry, temperature control system, and programmable acceleration and deceleration times.
Description • Dynamic Rotor Inertia Check (DRIC): As the rotor accelerates, rotor inertia is measured and the rotor energy is calculated for the speed set by the user. If the calculated rotor energy is determined to be excessive, the centrifuge recalculates a permitted set speed and uses this value to avoid possible rotor damage. A diagnostic message is displayed to indicate the change.
Description FRICTION REDUCTION SYSTEM (FRS) The friction reduction system (FRS) uses a mechanical rotary vane vacuum pump to reduce chamber pressure to approximately onequarter atmosphere (190 mm Hg). The pump turns on after the run is started, before rotor friction reaches a high level. When the required vacuum level is reached, the pump turns off. Vacuum in the chamber is vented during rotor deceleration.
Description NAME RATING PLATE The name rating plate is affixed to the rear of the centrifuge. Always mention the model number and serial number when corresponding with Beckman Coulter regarding your centrifuge. CONTROLS AND INDICATORS KEY AND POWER SWITCHES The key switch and the power switch are located below the control panel (see Figure 2-1).
Description • RUN LOCK mode disables all user inputs except [START] and [STOP] to ensure that current settings do not get changed. • INST(RUMENT) LOCK mode disables all user inputs except [STOP], to prevent use of the instrument after the current run is complete. CONTROL PANEL The control panel (Figure 2-2) is mounted at an angle on the centrifuge top rear for easy visibility and access.
Description ROTOR ID SPEED RPM/RCF TIME HR:MIN TEMP °C Figure 2-3. The Actual Value Display ROTOR ID Indicates the installed rotor. At the start of a run, this field is blank. During acceleration, the centrifuge identifies the installed rotor and displays the rotor name.
Description ROTOR SPEED Function Keys TIME Rotor : JA-30.50 Speed : - - Time : 00:00 Temp : 25°C Accel : MAX RPM = xG Max Temp : 29°C Decel : TEMP RPM RCF Soft Keys A/D OPT Message Line Figure 2-4. The Setup Screen When the centrifuge power is turned on, the setup screen displays the parameters of the last run performed. Function Keys Pressing [ENTER] or another function key saves parameter entries. [ROTOR] Pressed to select the rotor in use.
Description • Timed run (HH:MM) — Run time up to 99 hours and 59 minutes can be set. If more than 59 minutes are entered in the minutes field, the system automatically converts the entry to hours and minutes. Decelerating begins when the set time counts down to zero. The corresponding ω 2 t value can be displayed by pressing the W2T soft key. • ω 2 t run — An ω 2 t value can be entered and the system will calculate the run time required to reach that value.
Description [OPT] Pressed to put the centrifuge into program mode, where you can enter run parameters into memory for later use. Up to thirty two-step programs can be stored. KEYPAD 7 8 9 4 5 6 1 2 3 0 ±. CE E N T E R The keypad is used to enter numerical run parameters and to enter or recall a program number. In addition to keys 0 through 9, the keypad includes [±.] (plus/minus/decimal) and [CE] (clear entry) keys. The [±.
3 Operation This section contains detailed centrifuge operating procedures. A summary is provided at the start of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps. ! WARNING Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms.
Operation SUMMARY OF AVANTI J-26XPI RUN PROCEDURES For runs at other than room temperature, precool or prewarm the rotor to the required temperature before the run. MANUAL RUN 3-2 1 Press the POWER switch to on (I). 2 Depress the foot pedal to open the chamber door. 3 Install the rotor according to instructions in the applicable rotor manual, then firmly close the chamber door. 4 Press [ROTOR], press the soft key to select rotor type, press the soft key to select the rotor name.
Operation PROGRAMMED RUN 1 Press the POWER switch to on (I). 2 Depress the foot pedal to open the chamber door. 3 Install the rotor according to instructions in the applicable rotor manual, then firmly close the chamber door. 4 Press [OPT], then use the keypad to enter the program number. 5 Check that all parameters are correct and that the door is closed. Press [ENTER], then press [START]. 6 Wait for program step 1 or 2 to end, or end the run by pressing [STOP].
Operation Action Result ! 3. Install the rotor according to the instructions in the rotor manual. Make sure that the rotor is seated on the drive hub. 4. Securely attach the rotor lid knob, or tie-down knob in rotors without lids, to the drive shaft by turning it to the right (clockwise). ➠ ! 5. Close the chamber door. CAUTION Do not drop the rotor onto the drive hub. The drive spindle can be bent if the rotor is forced sideways or dropped onto the spindle hub.
Operation SELECTING A ROTOR Action Result 1. Press [ROTOR]. The Rotor: field flashes, and rotor type abbreviation soft keys (JA: fixed angle, JS: swinging bucket, JLA: lightweight fixed angle; JSP: special; JV: vertical tube; JE: elutriator; JCF-Z: continuous flow/ zonal) appear. 2. Press the appropriate soft key to select the rotor type. A list of rotor codes appears—fixed angle rotor codes are displayed below. Press the MORE soft key to display additional fixed angle rotor codes.
Operation Entering RPM Action Result 1. Press [SPEED]. The Speed: field flashes, and RPM and RCF soft keys appear. The RPM indicator illuminates in the actual value display. ROTOR ID SPEED RPM/RCF TIME HR:MIN TEMP °C RPM ROTOR SPEED TIME TEMP Rotor : Speed : Time : Temp : JA-30.50 -RPM 00:00 25°C Accel : MAX = -- x G RPM RCF Max Temp : 29°C Decel : MAX A/D OPT 2. Enter the required rpm on the keypad and press [ENTER]. The speed is displayed in the Speed: field.
Operation Action Result 2. Press the RCF soft key. The RPM = - - × G field flashes. ROTOR ID SPEED RPM/RCF TIME HR:MIN TEMP °C RCF (x g) ROTOR SPEED TIME Rotor : Speed : Time : Temp : JA-30.50 -RPM 00:00 25°C Accel : MAX = 81 800 x G RPM RCF Max Temp : 29°C Decel : TEMP A/D OPT 3. Enter the required rcf on the keypad and press [ENTER]. The rcf and the corresponding rpm appear in the Speed: field.
Operation Action Result 2. Use they keypad to enter the required time (up to 99 hours, 59 minutes) and press [ENTER]. Entered time appears in the Time: field. ROTOR SPEED TIME Rotor : Speed : Time : Temp : JA-30.50 26 000 RPM 00:25 25°C Accel : MAX = xG HH:MM W2T HOLD Max Temp : 29°C Decel : MAX TEMP A/D OPT 3. Press the next function key or press [ENTER], then [START]. The run begins, and the time remaining begins counting down to 0 in the TIME display.
Operation Action Result 3. Use the keypad to enter an ω 2 t value. The entry appears in the Time: field. The decimal point and E appear automatically as you type. If you enter a value out of the achievable ω 2 t range for that rotor, a message prompts you to enter a different value. ROTOR ID SPEED RPM/RCF TIME w2t TEMP °C EXP 03 rad2/sec ROTOR SPEED TIME Rotor : Speed : Time : Temp : JA-30.50 26 000 RPM 1.
Operation Action Result 2. Press the HOLD soft key to select the hold mode. HOLD appears in the Time: field. ROTOR ID SPEED TIME RPM/RCF TEMP HR:MIN °C HOLD ROTOR SPEED TIME TEMP Rotor : Speed : Time : Temp : Accel : JA-30.50 26 000 00:00 25°C MAX RPM = 81 800 x G HH:MM W2T HOLD Max Temp : 29°C Decel : MAX A/D OPT 3. Press the next function key or press [ENTER], then [START]. After you start the run, the HOLD indicator illuminates in the TIME display.
Operation Action Result 2. Use the keypad to enter the required temperature. Entered temperature appears in the Temp: field. 3. Press the [TEMP] key again to move the cursor to the Max Temp: field. Entered temperature plus 4°C appears in the Max Temp: field, and YES and NO soft keys appear. The default maximum temperature is the set temperature plus 4°C. You can specify a different maximum temperature, from +2°C above the set temperature to 44°C.
Operation Table 3-1. Acceleration and Deceleration Settings Setting Description Maximum acceleration (not available with the JS-24.38 and JS-24.15 rotors) Full torque is used from 0 rpm until set speed is reached. Slow acceleration Approximately 2 minutes from 0 to 500 rpm. (Time depends on the mass of the rotor in use; larger rotors take longer.) Above 500 rpm, full torque is used until set speed is reached. Timed acceleration Acceleration time from 0 to 500 rpm can be set from 1 to 10 minutes.
Operation Deceleration Action Result 1. Press [A/D] twice (if the cursor is in the Accel: field, press [A/D] once). The Decel: field flashes. MAX, SLOW, TIME, and OFF soft keys appear. 2. Press the appropriate soft key. If you choose MAX, SLOW, or OFF, your selection appears in the Decel: field. If you choose TIME, enter a deceleration time from 1 to 10 minutes and press [A/D] again or accept the 10-minute default setting. ROTOR SPEED TIME Rotor : Speed : Time : Temp : JA-30.
Operation ENDING A RUN To end a run in progress, press [STOP]. The red [STOP] indicator light flashes until the rotor comes to a complete stop. In timed runs, deceleration begins automatically when the TIME display counts down to zero. In ω 2 t runs, deceleration begins when the set ω 2 t is achieved. ➠ NOTE If a diagnostic condition causes the run to stop, a diagnostic message appears and the red [STOP] indicator light flashes until the rotor comes to a complete stop.
Operation Action Result 2. Press [OPT] again to movethe cursor to the Setup: field. Use the keypad to enter a program number in this field, then press [ENTER]. A two-step program is created and is assigned the number you entered. If you enter a number that is already assigned to a program, you can either overwrite the program or select another program number. A message prompts you to enter parameters for step 1. ROTOR TIME Rotor : Speed : Time : Temp : TEMP Accel : MAX Prog Recall: SPEED JA-30.
Operation Action Result 5. To add a second step to program 1, press the STEP2 soft key. 6. Enter parameters for step 2. Press [ENTER] when done. To recheck the parameters in either step, press the STEP1 or STEP2 soft key to toggle to that step. All parameters for step 1 and 2 are saved under the selected program number. ROTOR SPEED TIME TEMP Rotor : Speed : Time : Temp : JA-30.
Operation Action Result 2. Press [OPT] again to movethe cursor to the Setup: field. Enter the number of the program to be modified. The program number appears in the Setup: field. A message appears asking if you want to overwrite the program. YES and NO soft keys appear. ROTOR SPEED TIME TEMP Rotor : Speed : Time : Temp : JA-30.50 0 RPM = xG 00:00 25°C Max Temp : 29°C Shutdown? No YES NO Accel : MAX Decel : MAX Prog Recall: - Setup: 1 Step # Program number used, overwrite Y/N? A/D OPT 3.
Operation RUNNING A PROGRAM Action Result 1. Press [OPT]. The Prog Recall: field flashes. ROTOR TIME Rotor : Speed : Time : Temp : TEMP Accel : 5 Prog Recall: 1 SPEED JA-30.50 26 000 RPM = 81 800 xG 00:20 20°C Max Temp : 24°C Shutdown? No STEP1 STEP2 EXIT Decel : 5 Setup: Step #1 Enter program number (1Ð30) . A/D OPT 2. Use the keypad to enter the program number in the Prog Recall: field. 3. Press [ENTER], then [START].
Operation Action Result 1. Turn the key to the ZONAL position. Open-door operation up to 3000 rpm is enabled. JCF-Z appears in the Rotor: field. ROTOR ZONAL SPEED TIME Rotor : JCF-Z Speed : 0 RPM Time : 00:00 Temp : 25°C Accel : MAX = xG JCF-Z Max Temp : 29°C Decel : MAX TEMP A/D OPT 2. Install the rotor. Leave the door open when installation is complete. 3. Press [ROTOR]. Press the JCF-Z soft key. Enter the remaining run parameters.
Operation Action Result ! 8. Open the door and unload the rotor. 9. Press [STOP] to bring the rotor to a complete stop. ➠ WARNING Never try to slow or stop the rotor by hand. NOTE Before another zonal run can be started, you must press the RESET soft key. SETTING UP AN ELUTRIATION RUN Centrifuges equipped for elutriation have elutriation controls as shown in Figure 3-1, along with a view port in the door and an elutriation power box mounted on the back of the control panel. See the JE-5.
Operation Action Result 1. Install the rotor. 2. Set up sample and buffer reservoirs and tubing lines as described in the rotor manual. Route tubing lines through the elutriator port holes at the left side of the door seal. Close the centrifuge door. 3. Press [ROTOR]. Press the JE soft key. JE-5.0 appears in the Rotor: field. ROTOR SPEED TIME Rotor : Speed : Time : Temp : JE-5.0 0 00:00 25°C Accel : MAX RPM = 0 xG Max Temp : 29°C Decel : JA JS JLA JV JE JCF-Z TEMP A/D OPT 4.
Operation Action 7. When the rotor speed has stabilized, look through the port in the centrifuge door and turn the DELAY control knob until the elutriation chamber in the rotor is synchronized with the strobe. (The chamber will appear to be motionless). Result DELAY ELUTRIATOR Sample can now be injected into the lines. See the elutriator rotor instruction manual for detailed instructions.
4 Troubleshooting This section lists possible malfunctions, along with probable causes and corrective actions. Maintenance procedures are given in Section 5. For any problems not covered here, contact Beckman Coulter Field Service (1-800-742-2345 in the United States) for assistance. ➠ NOTE It is your responsibility to decontaminate the instrument, as well as any rotors and accessories, before requesting service by Beckman Coulter Field Service.
Troubleshooting • the diagnostic number and message, • the operating situation when the diagnostic condition occurred (such as rotor in use, speed, or load type), and • any unusual environmental and/or operating conditions (such as ambient temperature or voltage fluctuations). ➠ NOTE The information provided in Table 4-1 is a user guide, and is not a comprehensive checklist. Table 4-1.
Troubleshooting Table 4-1. Diagnostic Message Chart (continued) Diagnostic Number/ Message Problem Result Recommended Action L4/ Door problem, refer to manual Door stays locked after rotor stops spinning Sample cannot be retrieved Restart the centrifuge and perform a brief run. If door will still not open after decel, call Beckman Coulter Field Service.
Troubleshooting Table 4-1. Diagnostic Message Chart (continued) Diagnostic Number/ Message Problem Result Recommended Action S1/ System error There is a problem with the system control software, EPROM, or RAM System shuts down Call Beckman Coulter Field Service. H4/ Speed error a) a run is in progress (at speed or accelerating) The centrifuge does not accelerate, and the H4 diagnostic occurs. Press [STOP] (green light on [START] key will turn off), then press [CE] to clear the diagnostic.
Troubleshooting ACCESSING THE ROTOR IN CASE OF POWER FAILURE WARNING Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and/or mechanical injury. Therefore, turn the power off (O) and disconnect the instrument from the main power source, and refer such maintenance to qualified service personnel. ! WARNING Before performing this procedure, verify that the rotor is not spinning by listening carefully for any noise coming from the chamber.
Troubleshooting ROTOR SPEED TIME TEMP ROTOR: JA-20 TIME: 00:00 TEMP: 25°C ACCEL: MAX RPM RCF MAX TEMP : 29°C SHUTDOWN? YES DECEL: MAX A/D OPT 7 8 9 4 5 6 2 3 + CE . 1 0 E N T E R START MADE STOP IN U.S.A. Figure 4-1. Emergency Door Release Latch Access 4-6 Action Result 2. Insert a 4-mm or 5/32-in. Allen wrench straight through one of the holes (either one) and turn the wrench to the left (counterclockwise) about one-half turn. The latch disengages. 3.
Troubleshooting ROTOR ID SPEED RPM/RCF TIME HR:MIN TEMP °C RPM ROTOR SPEED ROTOR: JA-20 TIME: 00:00 TEMP: 25°C TIME ACCEL: MAX TEMP MAX TEMP : 29°C SHUTDOWN? YES RPM RCF DECEL: MAX A/D OPT 7 4 8 9 5 6 1 2 3 0 + CE . TM E N T E R START STOP Manual Door Release Interlock Lever Inner Front Panel Retaining Strip Figure 4-2. Manual Door Release Action Result 5.
Troubleshooting Action Result 7. To vent the chamber vacuum, grasp the red rubber hose and pull it up until it comes off of the pump fitting (see Figure 4-3). Use a back-andforth motion as you pull; this takes quite a bit of force. The vacuum will be completely vented several seconds after the hose is detached. ➠ ! NOTE Several tubing lines are tied to the red hose. As you grasp the hose, place your hand over these lines. Make sure that they stay connected as you pull.
Troubleshooting 1 Grasp red hose and pull up. Do not disconnect the tubing lines tied to the red hose. 2 Vacuum Pump Red hose removed from fitting 3 Replace red hose Figure 4-3.
Troubleshooting To replace the panel on the centrifuge, Action Result 1. Holding the panel at the top (with the instrument label facing out), insert the three grooved brackets on the bottom inside of the door over the front panel retaining strip (see Figure 4-4). Push the top of the front panel into place. Retaining Strip Figure 4-4. Front Panel Retaining Strip 4-10 2. Hold the left top edge in place and insert the allen wrench through the hole.
Troubleshooting JCF-Z ROTOR IDENTIFICATION The rotor identification system can, under two specific conditions, misidentify the JCF-Z continuous flow/zonal rotor. Misidentification has occurred if a rotor name other than JCF-Z appears in the ROTOR display when the centrifuge door is closed. If the rotor is misidentified, stop the run and follow one of the steps below.
5 Care and Maintenance This section describes routine care and maintenance procedures that you should perform regularly or as required. For maintenance not covered in this manual, contact Beckman Coulter Field Service.1 Refer to the applicable rotor manual and Rotors and Tubes for J Series Centrifuges (publication JR-IM) for instructions on the care of rotors and accessories.
Care and Maintenance MAINTENANCE Perform the following procedures regularly to ensure continued performance and long service life of the centrifuge. • Inspect the centrifuge chamber for accumulations of sample, dust, or glass particles from broken sample tubes. Clean as required (see CLEANING, below). • Check the air filter on the back panel for obstructions. Keep vents clear and clean. • Wipe condensation out of the rotor chamber between runs with a sponge or clean cloth to prevent chamber icing.
Care and Maintenance Tube Breakage If a glass tube breaks, and all the glass is not contained in the bucket or rotor, be sure to thoroughly clean the chamber. ! WARNING Be careful when examining or cleaning the chamber and chamber gasket, as sharp glass fragments may be embedded in their surfaces. • Examine the chamber gasket to make sure that no glass particles are retained in it. Carefully remove any glass particles that may remain. • Carefully wipe away any glass particles that remain in the chamber.
Care and Maintenance REPLACING THE AIR FILTER Check the air filter regularly and replace it about once a year, or more often if it looks dirty. The air filter is not fastened to the centrifuge, so no tools are required for removal or installation. 1. To remove the air filter, hold the side edges and lift the filter straight up until the bottom edge is above the centrifuge bottom retaining strip. Pull the filter out, bottom edge first, and discard it. Frame Edge Air Filter Frame Edge Retaining Strip 2.
Care and Maintenance RETURNING A CENTRIFUGE RGA Before returning a centrifuge or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the RGA form and for packaging and shipping instructions. To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from pathogens and/or radioactivity.
Care and Maintenance Zonal Tubing Adapter for Cole-Parmer size 15 tubing (9.6-mm [3/8-in.] O.D.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363845 Note: kit 366431 is also required. Tubing Adapter, stainless steel, for size 15 tubing (qty 2) . . . . . . . 363831 Cable Clamp, nylon, 9.6-mm (3/8-in.) I.D. (qty 10) . . . . . . . . . . . . 000596 Elutriation upgrade kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
APPENDIX Temperature Calibration Procedure The Avanti J-26XPI centrifuge specification for temperature control is ±2°C of the set temperature. That means that your sample will stay within 2°C of set temperature at all times, after rotor and system equilibration, described below. (During transient conditions, such as acceleration and deceleration, the rotor temperatures may be outside this range.) The following procedure is provided for those cases in which temperature control within ±1°C is required.
Temperature Calibration Procedure 5. After at least 1 hour, measure the temperature of the buffer or water using a thermometer or thermocouple. 6. If the measured and set temperatures are different, note how many degrees different the measurements are and adjust the set temperature up or down that number of degrees.
AVANTI® J SERIES CENTRIFUGE WARRANTY Subject to th e exceptions a nd up on the conditions specified below Beckman Coulter agrees to correct either by repair, or, at i ts el ection, by repl acement, any defects of material or workmanship which develop within o ne (1) year (3 years for the drive motor) after delivery of the Avanti® J Series Centrifuge centrifuge (the p roduct), to the original buyer by Beckman Coulter or by an aut horized representative, provided th at investigation an d factory inspection b
Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821 Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com ©2009 Beckman Coulter, Inc.
