Instructions For Use Avanti J-E High-Performance Centrifuge PN SJ-IM-8AC December 2013 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
Avanti J-E High-Performance Centrifuge PN SJ-IM-8AC (December 2013) © 2002–2013 Beckman Coulter, Inc. All rights reserved. Made in U.S.A. All rights reserved. No part of this document may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without prior written permission from Beckman Coulter, Inc. Beckman Coulter, the stylized logo, and Avanti are trademarks of Beckman Coulter, Inc. and are registered in the USPTO.
Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate the instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.
Safety Notice Electrical Safety Electrical Safety To reduce the risk of electrical shock, this instrument uses a three-wire electrical cord and plug to connect this equipment to earth-ground. To preserve this safety feature: • Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the centrifuge. • Never use a three-to-two wire plug adapter.
Safety Notice Chemical and Biological Safety Chemical and Biological Safety Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Such materials should not be used in this instrument, however, unless all necessary safety precautions are taken. • Observe all cautionary information printed on the original solution containers prior to their use. • Handle body fluids with care because they can transmit disease.
Safety Notice RoHS Notice indicate the product is recyclable. The date code on the label or product indicates the date of manufacture. China RoHS Environmental Label — This label indicates that the electronic information product does not contain any toxic or hazardous substances. The center “e” indicates the product is environmentally safe and does not have an Environmentally Friendly Use Period (EFUP) date. Therefore, it can safely be used indefinitely.
Contents Safety Notice, iii Introduction, xi CHAPTER 1: Introduction, 1-1 Centrifuge Function and Safety Features, 1-1 Centrifuge Function, 1-1 Safety Features, 1-2 Door, 1-2 Protective Ring, 1-2 Imbalance Detector, 1-3 Overspeed and Rotor Identification System, 1-3 Centrifuge Chassis, 1-3 Housing and Door, 1-3 Rotor Chamber, 1-3 Vacuum, 1-3 Temperature Control, 1-4 Drive, 1-4 Name Rating Plate, 1-4 Controls and Indicators, 1-4 Power Switch, 1-4 Control Panel, 1-4 Digital Displays, 1-5 Run-Parameter Keys,
Contents Selecting a Rotor, 2-5 Entering Run Speed, 2-5 Entering Run Time, 2-6 Entering Run Temperature, 2-6 Entering Acceleration and Deceleration Rates, 2-7 Starting a Run, 2-8 Programmed Operation, 2-8 Creating a New Program, 2-8 Starting a Programmed Run, 2-9 Recalling and Changing a Program, 2-10 Recalling a Program, 2-10 Changing a Program, 2-11 CHAPTER 3: Introduction, 3-1 User Messages, 3-1 Accessing the Rotor in Case of Power Failure, 3-4 CHAPTER 4: Introduction, 4-1 Instrument Care, 4-1 Maint
Illustrations Illustrations 1.1 The Avanti J-E Centrifuge, 1-2 1.2 The Control Panel, 1-5 3.1 Manual Door Release, 3-5 4.1 Air Filter Replacement, 4-4 A.1 Dimensions of the Avant J-E Centrifuge, A-2 A.
Tables Tables 3.
Introduction Certification Beckman Coulter Avanti J-E centrifuges are manufactured in a facility that maintains certifications to both ISO 9001:2008 and ISO 13485:2003. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies. Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com.
Introduction Conventions Conventions Certain symbols are used in this manual to call out safety related and other important information. These international symbols may also be displayed on the centrifuge and are reproduced on the inside back cover of this manual. Typographic Conventions Certain typographic conventions are used throughout this manual to distinguish names of user interface components, such as keys. • Key names (for example, START or PROGRAM) appear in bold face type.
CHAPTER 1 Description Introduction This chapter provides a brief physical and functional description of the Beckman Coulter Avanti J-E centrifuge. The operating controls and indicators are also described; instructions for their use are in CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in Chemical Resistances (publication IN-175). Refer to the applicable rotor manuals for rotor descriptions.
Description Centrifuge Function and Safety Features Figure 1.1 The Avanti J-E Centrifuge Manual and programmed operation are available. • In manual operation, you enter the individual run parameters before beginning each run. • In programmed operation, you can duplicate runs quickly and accurately by selecting one of nine programs previously entered into the program memory. User messages and/or audible signals are provided to alert you to conditions that may need attention.
Description Centrifuge Chassis Imbalance Detector An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor loads are severely out of balance. At low speeds, an incorrectly loaded rotor can cause an imbalance. Rotor instability can also occur if the instrument is moved, or if it is not resting level on the floor (see CHAPTER 3, Troubleshooting).
Description Name Rating Plate Temperature Control The temperature control system cools by circulation of a non-CFC-based refrigerant. The system is activated when the centrifuge power is on and when the door is closed and latched. A thermistor in the rotor chamber continuously monitors the chamber temperature. The system calculates the chamber temperature required to maintain the set rotor temperature, ±2°C.
Description Controls and Indicators Figure 1.2 The Control Panel Digital Displays The displays provide run information, user messages, and diagnostics. • During a run, they show the actual (real-time) operating conditions. • When the run parameters are being entered, they show the set values selected. Set values can be recalled during operation by pressing SHOW SET. After 5 seconds, the displays return to actual conditions.
Description Controls and Indicators DECEL When the DECEL key is pressed, a number representing the selected deceleration rate (1 = MAX, 2 = SLOW, 3 = OFF) can be entered. If no number is entered, the instrument decelerates at the rate set for the previous run. PROGRAM • During setup — when the PROGRAM key is pressed a program number (one of nine possible) can be entered.
Description Controls and Indicators The keypad is used to enter numerical run parameters, to enter or recall a program number, and to select acceleration and/or deceleration rates. In addition to keys 0 through 9, the keypad includes plus or minus/decimal key and a CE (clear entry) key. The ± key is used to enter a temperature setting below 0°C (for example, –2°C), or to enter a decimal point in a rotor entry code, depending on the parameter key selected.
Description Specifications Specifications Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee.
Description Specifications Specification Description Finishes • Control panel: — silicone elastomer • Top surface: — urethane paint • Other surfaces: — acrylic baking enamel Electrical requirements • 208/240-V instrument: — 187–229/216–264 VAC, 24 A, 60 Hz • 230-V instrument: — 207–253 VAC, 24 A, 50 Hz • 200-V instrument: — 180–220 VAC, 24 A, 50 Hz Electrical supply Class I Maximum heat dissipation into room under steady-state conditions (JA-20 rotor) 2 kW (~6900 BTU/h) Noise level 0.
Description Available Rotors Available Rotors Refer to the applicable rotor manual for complete rotor descriptions. Rotor Profile 1-10 Description Rotor Code Max RPMa Max RCF (´ g) Max Capacity Rotor Manual Number JA-25.50 Fixed Angle, 34° (8 place) rmax = 108 mm 25.5 21,000b 53,300 8 ´ 50 mL J-TB-056 JA-21 Fixed Angle, 40° (18 place) rmax = 102 mm 21 50,400 18 ´ 10 mL J-TB-002 JA-20.1 Fixed Angle, 23° (32 place) rmax = 115 mm (outer row) rmax = 98 mm (inner row) 20.
Description Available Rotors Rotor Profile PN SJ-IM-8AC Description Rotor Code Max RPMa Max RCF (´ g) Max Capacity Rotor Manual Number JLA-16.250 Fixed Angle, 25° (6 place) rmax = 134 mm 16.
Description Available Rotors Rotor Profile Description Rotor Code Max RPMa Max RCF (´ g) Max Capacity Rotor Manual Number JS-13.1 Swinging Bucket (6 place) rmax = 140 mm 13 13,000 26,500 6 ´ 50 mL J-TB-036 JS-5.3 Swinging Bucket (4 place) rmax = 194.8 mm 5.3 6130 4 ´ 500 mL 24 microplates 8 deep-well plates 4 square-well plates J-TB-089 (12,000 @ 2°C)c 5300 a. Maximum speeds are based on a solution density of 1.2 g/mL in all rotors. b.
CHAPTER 2 Operation Introduction This section contains manual and programmed operating procedures. A summary is provided at the start of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps. WARNING Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Handle body fluids with care because they can transmit disease.
Operation Summary of Avanti J-E Run Procedures Programmed Run 1 Turn the POWER switch on (I). 2 Press DOOR to unlock the chamber door; lift the door open. 3 4 5 6 7 Install the rotor according to the applicable rotor manual, then close the chamber door and press DOOR. Press PROGRAM, use the keypad to enter the required program number, then press ENTER. Press SHOW SET to display program parameters. Check that all parameters are correct and that the door is closed.
Operation Preparation 6 Press TIME, then use the keypad to enter the run time (to 99 hrs, 59 minutes); or press TIME twice for a hold (continuous) run. 7 Press TEMP°C, then use the keypad to enter the required run temperature (–10 to +40°C). 8 Press ACCEL, then use the keypad to enter the selected acceleration rate number, 1 or 2. 9 Press DECEL then use the keypad to enter the selected deceleration rate number, 1, 2, or 3. 10 Check that all parameters are correct and that the door is closed.
Operation Preparation 4 Install the rotor according to directions in the rotor manual. a. Ensure that the rotor is seated on the drive hub. b. Securely attach the rotor lid knob, or tie-down knob in rotors without lids, to the drive shaft by turning it to the right (clockwise). NOTE If the knob turns loosely and you do not feel threads engaging, the rotor drive hole pins may not be properly seated on the centrifuge hub.
Operation Manual Operation Manual Operation When a run-parameter key (ROTOR, RPM/RCF, TIME, TEMP, ACCEL, or DECEL) is pressed, a parameter can be entered or changed. Entry is completed when ENTER or another function key is pressed. 1 To change an entry before you’ve pressed ENTER or another parameter key, press CE and enter a different value. a. To change an entry after you’ve pressed ENTER, press the run-parameter key again.
Operation Manual Operation If the entered speed is higher than the rated speed of the installed rotor, a message showing the valid speed range will be displayed. a. Press CE and enter an acceptable value. 3 Press the next run-parameter key or ENTER. You can change the set speed at any time by repeating steps 1 through 3. The rotor will accelerate or decelerate to the new speed. NOTE During acceleration, the instrument identifies the rotor and checks its maximum allowable speed.
Operation Manual Operation 2 Use the keypad to enter required temperature. Entered temperature appears on the display. (If the entered temperature is lower than can be achieved for the installed rotor, a message showing “Input error temp” will be displayed.) 3 Press the next run-parameter key or ENTER. Entering Acceleration and Deceleration Rates The instrument provides a choice of two acceleration rates and three deceleration rates to protect the gradient and sample-to-gradient interface.
Operation Programmed Operation Starting a Run 1 Press ENTER and START. The green light next to the START key flashes and the rotor begins to spin. NOTE To begin a run, ENTER must always be the last key pressed before pressing START. If you wait more than 5 seconds, the START key will not activate. If this happens, press ENTER and START again to begin the run. If an unacceptable value was entered, the run will not begin when ENTER and START are pressed. a.
Operation Programmed Operation 2 Use the keypad to select a program number that is not in use or one that you want to overwrite and press ENTER. The message “Press ENTER START to run, or edit parameters” is displayed. 3 Enter run parameters (speed, time, temperature, acceleration/deceleration rate settings). If you entered an unacceptable parameter, an error message will be displayed. a. Press CE and enter an acceptable value, then press the next run-parameter key.
Operation Programmed Operation If an unacceptable value was entered, the run will not begin when ENTER and START are pressed. a. Check each display and make any necessary corrections or additions. b. Press ENTER and START again to begin the run. The run will end automatically when the time display counts down to zero. c. Press STOP to terminate a run at any time. The green light next to the STOP key will flash while the rotor decelerates. A tone will sound when the rotor has come to a complete stop.
Operation Programmed Operation Changing a Program 1 Press PROGRAM. Previously saved program numbers are displayed. 2 Select the appropriate keypad number and press ENTER. The message “Press ENTER START to run, or edit parameters” is displayed. 3 Press SHOW SET to display program parameters. 4 Press the run parameter key to be changed. Parameter prompts are displayed. 5 Use the keypad to enter the new value. 6 Repeat steps 4 and 5 to change other run parameters. a.
Operation Programmed Operation 2-12 PN SJ-IM-8AC
CHAPTER 3 Troubleshooting Introduction This section lists possible malfunctions, along with probable causes and corrective actions. Maintenance procedures are given in CHAPTER 4, Care and Maintenance. For any problems not covered here, call Beckman Coulter Customer Service at 1-800-742-2345 (U.S.A. or Canada) or contact your local Beckman Coulter Representative for assistance.
Troubleshooting User Messages Table 3.1 Diagnostic Message Chart Diagnostic Message Problem Result Recommendation P1– Power failure occurred, see manual Momentary power failure: rotor does not come to a complete stop Run continues when power resumes Press CE to clear message. P2– Power failure, see manual Power failure: rotor speed drops to <500 rpm Run restarts automatically when power resumes Press CE to clear message.
Troubleshooting User Messages Table 3.1 Diagnostic Message Chart (Continued) Diagnostic Message Problem Fl and F2– FRS,b call service Required vacuum level not Run shuts down with reached in allowed time maximum brake • Check and clean door sealing area and door gasket. • Wipe any ice and excess moisture from chamber. • Call Beckman Coulter Customer Service.
Troubleshooting Accessing the Rotor in Case of Power Failure Table 3.1 Diagnostic Message Chart (Continued) Diagnostic Message Problem Result Recommendation l1Rotor imbalance Rotor load is severely out of balance Run shuts sown with maximum brake • Make sure that tubes or bottles are loaded symmetrically in the rotor. • With swinging bucket rotors, remove the buckets and lubricate the pivot pins where the buckets contact as described in the applicable rotor manual.
Troubleshooting Accessing the Rotor in Case of Power Failure 2 Insert the hex wrench (729317) or a similar tool into the openings on each side of the centrifuge (see Figure 3.1) to release the top front panel. CAUTION LISTEN CAREFULY. Do not proceed if any sound or vibration is coming from the drive. Figure 3.1 Manual Door Release 1 2 TM 1 1. Hex Wrench 3 2. Latch Override Key Lower the panel as far as the tether cords allow. CAUTION Do not attempt to remove tether cords.
Troubleshooting Accessing the Rotor in Case of Power Failure 5 After removing the rotor, replace the top front panel by pushing it into place. WARNING NEVER try to slow or stop the rotor by hand.
CHAPTER 4 Care and Maintenance Introduction This section describes routine care and maintenance procedures that you should perform regularly. For maintenance not covered in this manual, call Beckman Customer Service at 1-800-742-2345 (U.S.A. or Canada) or contact your local Beckman Coulter office for assistance. User messages and recommended actions are discussed in CHAPTER 3, Troubleshooting.
Care and Maintenance Maintenance 2 Check the air filter on the back panel for obstructions. Keep vents clear and clean. 3 4 Wipe condensation out of the chamber between runs with a sponge or clean cloth to prevent chamber icing. If chamber icing occurs, defrost the system and wipe moisture out of the chamber before use. To defrost the system, set the temperature to 30°C for 20 minutes. (These are suggested settings that may be adjusted as appropriate for your laboratory conditions.
Care and Maintenance Maintenance Tube Breakage If a glass tube breaks, and all the glass is not contained in the bucket or rotor, be sure to thoroughly clean the chamber. WARNING Be careful when examining or cleaning the chamber and chamber gasket, as sharp glass fragments may be embedded in their surfaces. 1 Examine the chamber gasket to make sure that no glass particles are retained in it. Carefully remove any glass particles that may remain.
Care and Maintenance Maintenance Replacing the Air Filter 1 Check the air filter (see Figure 4.1) regularly and replace it about once a year, or more often if it looks dirty. The air filter is not fastened to the centrifuge, so no tools are required for removal or installation. 2 3 To remove the air filter, grasp the top edge and lift the filter straight up. Install a new filter by holding the top edge and inserting the bottom edge into the slot in the air deflector strip.
Care and Maintenance Circuit Breaker and Fuses Circuit Breaker and Fuses There are no user-replaceable fuses in the Avanti J-E centrifuge. If the centrifuge circuit breaker trips for any reason, the power switch will move to the OFF (O) position. Reset the circuit breaker by turning the power switch back to the ON (I) position. If it trips again immediately, do not reset it. Call Beckman Coulter Field Service.
Care and Maintenance Supply List Replacement Parts Description Part Number Air filter 978347 Latch override key 368247 Memory flash card 369173 Supplies NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.
APPENDIX A Preinstallation Requirements Introduction Preinstallation requirements have been provided for your Avanti J-E centrifuge. The following information is included in case the instrument must be relocated. (After the centrifuge is moved it must be leveled by adjusting the two front feet.) NOTE This centrifuge is designed to be installed by a Beckman Coulter Field Service representative.
Preinstallation Requirements Space Requirements 3 4 In addition to space for the instrument itself, allow a 7.7-cm (3-in.) clearance on each side of the instrument and a 16-cm (6.25-in.) clearance behind the instrument for air circulation. Position the centrifuge so that the air diverter nearly touches the wall behind the centrifuge (see Figure A.1). a. Place the power cord to one side of the air diverter. b.
Preinstallation Requirements Electrical Requirements Electrical Requirements 200-V centrifuge 180–220 VAC, 24 A, 50/60 Hz 230-V centrifuge 207–253 VAC, 24 A, 50/60 Hz 208/240-V centrifuge 187–229/216–264 VAC, 24 A, 50 Hz To reduce the risk of electrical shock, this centrifuge uses a permanently attached 1.8-m (6-ft) three-wire UL/CSA approved electrical cord and plug to connect the centrifuge to earth-ground.
Preinstallation Requirements Securing the Centrifuge to the Floor • Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet receptacle strip. • If there is any question about voltage, have a qualified service person measure it under load while the drive is operating. To ensure safety the centrifuge should be wired to a remote emergency switch in order to disconnect the centrifuge from the main power source in case of a malfunction.
Beckman Coulter, Inc. Avanti J-E Centrifuge Warranty Subject to the exceptions and upon the conditions specified below Beckman Coulter, Inc.
Beckman Coulter, Inc.
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Related Documents Rotors and Tubes for Beckman Coulter J2, J6,and Avanti J Series Centrifuges PN JR-IM-10 • Rotors • Tubes, Bottles, and Accessories • Using Tubes and Accessories • Using Fixed-Angle Rotors • Using Swinging-Bucket Rotors • Using Vertical-Tube and Rack Type Rotors • Care and Maintenance • Chemical Resistances • Temperature Compensation Tables • Gradient Materials • Blood Component Separation High-Performance and HighCapacity Centrifuges Catalog PN BR-8102 Available at www.beckmancoulter.
