Technical information

Centrifuge Standard Operating Procedure 14
the drive spindle hub. There are arrows on the rotor to direct
placement of the rotor on the spindle.
d. When the yoke is correctly seated, secure it to the drive spindle hub
by hand tightening the tie-down knob. If the rotor is left in the
centrifuge between runs, tighten the knob before each run.
e. Close the centrifuge door only when you are sure the samples and
rotor have been correctly balanced and attached to the centrifuge;
f. The centrifuge will not automatically recognize the JA 13.1 rotor; it
must be set using the “ROTOR” key on the control panel, and a soft
key to select the rotor name. Press “ENTER”;
6. If using the JLA 10.500 rotor:
a. Install the rotor body, making sure that the rotor alignment pins
are properly positioned between the teeth on the centrifuge drive
shaft; if the pins are not aligned properly massive damage can result;
b. Make sure all of the rotor buckets are firmly seated in the rotor body
(even if only using 2 buckets, all must be in place and capped);
c. Load samples into the rotor buckets, making sure all samples are
properly balanced;
d. Hand-tighten a closure onto each bucket (do not use any wrench or
tool, this will result in over-tightening and potentially damage the
buckets);
e. Place the lid on the rotor and use the knob to securely fasten the
lid/rotor assembly to the centrifuge drive shaft (check this by lifting
up on the rotor);
f. Close the centrifuge door only when you are sure the samples and
rotor have been correctly balanced and attached to the centrifuge;
g. The centrifuge will automatically recognize the JLA 10.500 rotor.
7. Enter the speed required for the run. Press the “SPEED” button, choose
RPMs (revolutions per minute) or RCF (relative centrifugal force; the
measurement of the force applied to a sample within a centrifuge) using the
soft keys, and then enter the desired number. Press “ENTER”;
8. Set the time required for the run. Press the “TIME” key, and then choose
the type of time needed using the soft keys, the choices being
Hours:Minutes (HH:MM), Hold (centrifuge will spin until you turn it off), or
2t mode (accumulated centrifugal force); if HH:MM or 2t mode is chosen,
enter the appropriate numbers followed by “ENTER”;
9. Set the temp required for the run (typically it is set at 4 ˚C). Press “TEMP”
and enter the desired temperature in degrees Celsius, followed by “ENTER”;
10.Set the acceleration rate. Press "A/D” and choose the appropriate soft key:
“MAX” (full acceleration), “SLOW” (slow acceleration from 0-500 RPM, and
then full acceleration) or “TIME” (acceleration from 0-500 RPM can be
programmed for 1-10 minutes, followed by full acceleration). Press “Enter”;
11.Set the deceleration rate. Press ”A/D” once or twice (until the cursor blinks
in the first digit of the “DECEL” field) and choose the appropriate soft key:
“MAX” (full deceleration), “SLOW” (reduced deceleration from set speed to
500 RPM, and then about 2 minutes to come to a full stop), “TIME” (full
deceleration from set speed to 500 RPM , and then deceleration from 500 to