Technical information

Centrifuge Standard Operating Procedure ii
TABLE OF CONTENTS
DISCLAIMER ................................................................................ iv
ACKNOWLEDGEMENTS .....................................................................v
1. INTRODUCTION........................................................................1
1.1 Purpose of the Standard Operating Procedure .............................1
1.2 Centrifugation Theory..........................................................1
1.2.1 Centrifugation Methods...................................................2
1.2.2 Rotor Selection ............................................................3
1.3 Instrumentation .................................................................4
2. POTENTIAL HAZARDS.................................................................7
3. PERSONAL PROTECTIVE EQUIPMENT ...............................................8
4. SPILL AND ACCIDENT PROCEDURES ................................................9
4.1 Accidents.........................................................................9
4.2 Spills ..............................................................................9
4.2.1 Spills Inside the Centrifuge ..............................................9
4.2.2 Spills Outside the Centrifuge ............................................9
5. WASTE DISPOSAL PROCEDURES ................................................... 11
6. PROTOCOL........................................................................... 12
6.1 Sample Preparation........................................................... 12
6.2 Running the Centrifuge ...................................................... 13
6.3 After Completing a Run ...................................................... 15
7. TROUBLESHOOTING ................................................................ 16
7.1 Equipment Malfunction ...................................................... 16
8. PREVENTATIVE MAINTENANCE .................................................... 19
8.1 Daily ............................................................................ 19
8.2 Weekly.......................................................................... 19
8.3 Monthly ......................................................................... 19
8.4 Three Months .................................................................. 19
8.5 Six Months...................................................................... 19
8.6 Annually ........................................................................ 19
9. QUICK REFERENCE GUIDE.......................................................... 20
10. REFERENCES ...................................................................... 21