User manual
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For further details see below including information on particular connector boxes, see below.
Perfusion Channel System Port PCI6221 CB-68LPR USB-6221 BNC
Standard8 Standard16 PreFlush4 PreFlush8 Bit# Pin# (CA1000) SCB-68 BNC-2090A BNC-2110 BNC-2120 USB-6341 BNC
1-chamber 1-chamber 1-chamber 1-chamber P1.0 11 PFI0 11 PFI0 PFI0 PFI0/P1.0 P1.0/PFI0 P1.0/PFI0
2-chamber 2-chamber 2-chamber 2-chamber P1.1 10 PFI1 10 PFI1 PFI1 PFI1/P1.1 P1.1/PFI1 P1.1/PFI1
3-chamber 3-chamber 3-chamber 3-chamber P1.2 43 PFI2 43 PFI2 PFI2 PFI2/P1.2 P1.2/PFI2 P1.2/PFI2
4-chamber 4-chamber 4-chamber 4-chamber P1.3 42 PFI3 42 PFI3 PFI3 PFI3/P1.3 P1.3/PFI3 P1.3/PFI3
5-chamber 5-chamber 1-flush 5-chamber P1.4 41 PFI4 41 PFI4 PFI4 PFI4/P1.4 P1.4/PFI4 P1.4/PFI4
6-chamber 6-chamber 2-flush 6-chamber P1.5 6 PFI5 6 PFI5 PFI5 PFI5/P1.5 P1.5/PFI5 P1.5/PFI5
7-chamber 7-chamber 3-flush 7-chamber P1.6 5 PFI6 5 PFI6 PFI6 PFI6/P1.6 P1.6/PFI6 P1.6/PFI6
8-chamber 8-chamber 4-flush 8-chamber P1.7 38 PFI7 38 PFI7 PFI7 PFI7/P1.7 P1.7/PFI7 P1.7/PFI7
9-chamber 1-flush P2.0 37 PFI8 37 PFI8 PFI8 PFI8/P2.0 P1.0/PFI8 P2.0/PFI8
10-chamber 2-flush P2.1 3 PFI9 3 PFI9 PFI9 PFI9/P2.1 P1.1/PFI9 P2.1/PFI9
11-chamber 3-flush P2.2 45 PFI10 45 EXT STROBE PFI10 EXTSTRB or PF10/P2.2 NA P2.2/PFI10
12-chamber 4-flush P2.3 46 PFI11 46 AI HOLD COMP PFI11 AI HOLD or PF11/P2.3 NA P2.3/PFI11
13-chamber 5-flush P2.4 2 PFI12 2 CTR 0 OUT PFI12 CTR 0 OUT or PF12/P2.4 P1.4/PFI12 P2.4/PFI12
14-chamber 6-flush P2.5 40 PFI13 40 CTR 1 OUT PFI13 CTR 1 OUT or PF13/P2.5 P1.5/PFI13 P2.5/PFI13
15-chamber 7-flush P2.6 1 PFI14 1 FREQ OUT PFI14 F OUT or PF14/P2.6 P1.6/PFI14 P2.6/PFI14
16-chamber 8-flush P2.7 39 PFI15 39 P2.7,not DGND PFI15 NA NA P2.7/PFI15
1) In the SCB-68, sometimes Pin39 is labelled DGND rather than the correct P2.7
2) There is no P2.7 output with the BNC-2110
3) There are no P2.2, P2.3 or P2.7 outputs with the BNC-2120
4) The SCB-68, BNC-2090A, USB-6221 BNC, USB-6341 BNC and the CB-68LPR (for the CA-1000 enclosure) do have all Port1 and Port2 pinouts
Note that there is no P2.7 (PFI 15) output with the BNC-2110, so you can’t use channel 16 with the
standard 16-channel system, or channel 8 with the pre-flush 8-channel system, so the BNC-2110 is
not a good choice for an Automatic Perfusion System.
Similarly, there is no P2.2, P2.3 or P2.7 output with the BNC-2120, so you can’t use channel 11, 12
and 16 with the standard 16-channel system, or channel 3, 4 and 8 with the pre-flush 8-channel so
the BNC-2120 is not a good choice for an Automatic Perfusion System.
Use the SCB-68, BNC-2090A, USB-6341 BNC, or the CB-68LPR (for the CA-1000 enclosure) if you need
to use all outputs from Port1 and Port2.
Then build your perfusion system according to the instructions of the manufacturer. Basically, for the
standard perfusion system with 1 valve/line, run the tubing from the reservoir through the pinch valve to
the manifold and onto the chamber as shown in Fig 10.2.4.1A. For the pre-flush perfusion system with 2
valves/line (such as the AutoPrime system from AutoMate Scientific), run the tubing from the reservoir to
a T-fitting, and run tubing through the Chamber pinch valve to the manifold and onto the chamber, and
run the other tubing through the Flush pinch valve and onto the waste receptacle as shown in Fig
10.2.4.1B.
For extracellular slice experiments, all perfusion controllers can control the more inexpensive pinch-
valves. The faster Lee-valves etc. are not necessary and are more expensive and difficult to clean and
maintain.