User manual

227
The rules for Adding Events and printing the PxSweep immediately following the AddedEvent are
summarized as follows:
1) The PxSweep following the AddedEvent will only be available during the current "Start MainProtool".
2) The PxSweep following the AddedEvent will only be available for the last 5000 sweeps, anything
eariler will be lost and not printed.
3) It is OK to AddEvent after the MainProtocol has stopped as long as another MainProtocol has not
been started.
4) If you do several AddedEvents, only the PxSweep following the first AddedEvent will be printed to
the Expimental Log.
11.8 Printing SealTest protocol values
The Experimental Log will also automatically record important values such as those obtained from the
patch-clamp SealTest protocol (see Chapter 6). These values include:
1) with the electrode in the bath
a) the resistance of the pipette (Rpipette), in Mohms
2) after the seal has formed
a) the seal resistance (Rseal), in Gohms
3) After going whole cell
a) the patch pipette series resistance (Rs), in Mohms
b) the cell input resistance (Rm), in Mohms
c) the holding current (Idc), in pA’s
and also the pulse amplitude (PulseAmp), in mV’s, used to generate the SealTest values (Fig. 11.8.1).
11:44:05.4 "" Go to SealTest
"" "" ElectrodeInBath
"" "" AD0 Rpipette = 4.1 Mohms PulseAmp = 2.0 mV
"" "" FormSeal
"" "" AD0 Rseal = 1.5 Gohms PulseAmp = 20.0 mV
"" "" GoWholeCell
"" "" AD0 Rs = 12 Mohms PulseAmp = -2.0 mV
"" "" Rm = 190 Mohms
"" "" Idc = -28 pA
11:46:54.7 "" Return from SealTest
Fig. 11.8.1. The results of the SealTest protocol printed in the Experimental Log after exiting the
SealTest.
11.9 Printing Load/Save Protocol Files, Save AmpFiles, Clear
AnalysisGraphs and Spreadsheets
Finally, the Experimental Log records
1) when protocol files are loaded and saved
2) when AmpFiles are saved
3) when AnalysisGraphs and Spreadsheets are cleared