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COULTER LH 750 System

Reference

PN 4277248DD (January 2013)

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821 U.S.A.

*SYSTEM RUN*

04/30/96

12:30:18

STATUS

: READY

ALERT

MODE: C/D/R

BLOOD DETECT: ON

TEST MODE

ASPIRATIONS/TUBE 01

MAIN MENU

VIII

VII

III

POWER ON

POWER OFF

START UP

SHUT DOWN

ALARM

RESET

READY

PRIME

APERT

DRAIN RINSE

CLEAR

APERT

1F 2

STOP PREMIX

START

CONT

NEC

MultiSync LCD 1700m+

Summary of content (120 pages)

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COULTER LH 750 System Reference *SYSTEM RUN* 04/30/96 12:30:18 I II III IV STATUS : READY ALERT: MODE: C/D/R V TEST MODE ASPIRATIONS/TUBE 01 PRIME CLEAR DRAIN RINSE APERT APERT START UP SHUT DOWN F VIII STOP PREMIX READY 1 4 7 CE VI VII MAIN MENU BLOOD DETECT: ON 2 5 8 0 3 6 9 . ID E N T E R START ALARM CONT RESET POWER ON POWER OFF NEC PN 4277248DD (January 2013) Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
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WARNINGS AND PRECAUTIONS READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTING TO OPERATE INSTRUMENT. DO NOT ATTEMPT TO PERFORM ANY PROCEDURE BEFORE CAREFULLY READING ALL INSTRUCTIONS. ALWAYS FOLLOW PRODUCT LABELING AND MANUFACTURER’S RECOMMENDATIONS. IF IN DOUBT AS TO HOW TO PROCEED IN ANY SITUATION, CONTACT YOUR BECKMAN COULTER REPRESENTATIVE.
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REVISION STATUS Issue A, 10/01 Software version 1A. Converted from Help Version 1A.012431. Issue B, Complete Revision, 5/02 Software version 2A. Converted from Help Version 2A.021501. Issue C, 10/03 Software version 2B. Converted from Help Version 2B.031971. Changes were made to: r Change the company name from Coulter Corporation to Beckman Coulter Inc. r Change LH 750 to LH 700 Series. r Change all variations of bar-code to be consistent. r Change 5C-ES to 5C Series.
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REVISION STATUS Issue DD, 01/13 Software Version 2D3. Manual derived from Online Help version 2D3. Changes were made to: r SPECIAL REQUIREMENTS: HARDWARE r Space and Accessibility r Pneumatic/Hydraulic Tubing Connections r Changing 9-pin and 25-pin connectors r Added reference to the Hematology Tube List information Note: Changes that are part of the most recent revision are indicated in text by a bar in the margin of the amended page.
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CONTENTS WARNINGS AND PRECAUTIONS, ii REVISION STATUS, iii CONTENTS, v INTRODUCTION, xiii HOW TO USE YOUR COULTER LH 750 SERIES SYSTEM HARD-COPY MANUALS, xiii ABOUT THIS MANUAL, xiv ONLINE HELP SYSTEM, xiv CONVENTIONS, xiv 1 PN 4277248DD USE AND FUNCTION, 1-1 1.1 INTENDED USE, 1-1 1.2 INDICATIONS FOR USE, 1-1 Parameters, 1-2 1.3 QUALITY CONTROL (QC), 1-3 1.
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CONTENTS 2 3 vi 1.7 CONTROLS AND CALIBRATOR, 1-9 Controls, 1-9 Calibrator, 1-9 1.8 REAGENTS, 1-9 Diluent, 1-10 CBC Lytic Reagent, 1-10 LH Series PAK Reagent System, 1-10 The LH Series RETIC PAK Reagent Kit, 1-10 Cleaners, 1-11 1.9 MATERIAL SAFETY DATA SHEETS (MSDS), 1-11 INSTALLATION, 2-1 2.1 GENERAL, 2-1 2.2 SPECIAL REQUIREMENTS: HARDWARE, 2-1 Space and Accessibility, 2-1 Electrical Input, 2-2 Ambient Temperature and Humidity, 2-3 Air Conditioning, 2-3 Ventilation, 2-3 Drainage, 2-3 2.
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CONTENTS PN 4277248DD 3.4 COUNTING AND SIZING, 3-9 Red and White Blood Cell Counting, 3-9 Coincidence Correction, 3-10 Voting, 3-10 Pulse Editing, 3-10 Sweep Flow, 3-10 RBC Size Distribution, 3-11 Platelet Method, 3-11 3.5 MEASUREMENT OF HEMOGLOBIN CONCENTRATION, 3-12 3.6 DATAPLOT DEVELOPMENT, 3-13 Two-Dimensional (2D) DataPlots, 3-13 Three-Dimensional (3D) DataPlots, 3-13 3.
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CONTENTS 4 viii SPECIFICATIONS/CHARACTERISTICS, 4-1 4.1 PHYSICAL SPECIFICATIONS, 4-1 Dimensions, 4-1 Power, 4-1 Input, 4-1 Consumption, 4-1 4.2 PERFORMANCE SPECIFICATIONS--LH 700 Series, 4-1 Precision, 4-1 Within-Run Precision, 4-1 Accuracy, 4-2 Accuracy Qualification, 4-2 Accuracy, CBC, 4-2 Accuracy, WBC Differential, 4-3 Accuracy, Reticulocyte, 4-3 Linearity, 4-3 Background, 4-4 Carryover, 4-4 Operating and Reportable Ranges, 4-4 Mode-to-Mode Comparison, 4-5 4.
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CONTENTS 5 PN 4277248DD 4.4 BAR-CODE SYMBOLOGY OVERVIEW, 4-14 Character Set, 4-15 Symbology Type, 4-15 Fixed or Variable Length, 4-15 Self-Checking, 4-15 Start Code, Stop Code, 4-15 Check Character, Checksum Algorithm, 4-15 Quiet Zone, Quiet Area, 4-15 4.
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CONTENTS REFERENCES, REFERENCES-1 GLOSSARY, GLOSSARY-1 INDEX, INDEX-1 BECKMAN COULTER, INC.
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CONTENTS ILLUSTRATIONS 1.1 1.2 1.3 1.4 1.5 2.1 2.2 2.3 2.4 2.5 3.1 3.2 3.3 3.4 3.5 4.1 5.1 5.2 5.3 5.4 5.
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CONTENTS TABLES 2.1 3.1 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 4.10 4.13 4.11 4.12 4.15 4.16 4.14 4.17 4.18 4.19 4.20 4.21 4.22 4.23 4.24 4.25 4.26 4.27 4.
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INTRODUCTION This introductory section contains the following topics: r How to use your COULTER LH 750 System hard-copy manuals r About this manual r Online Help System r Conventions HOW TO USE YOUR COULTER LH 750 SERIES SYSTEM HARD-COPY MANUALS Use the Getting Started booklet to see an overview of the system hardware and software. This document comes with your LH 750 System.
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INTRODUCTION ABOUT THIS MANUAL ABOUT THIS MANUAL Your LH 750 System Reference Guide is a source of information for the day-to-day operation of your instrument. This information is organized as follows: s Chapter 1, Use and Function Contains the intended use of the instrument, a brief history of the methods used, the reagents, calibrators and controls used, and a short description of the major components.
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1USE AND FUNCTION 1 1.1 INTENDED USE The COULTER LH 750 Hematology Analyzer is a quantitative, automated hematology analyzer and leukocyte differential counter For In Vitro Diagnostic Use in clinical laboratories. The COULTER LH 750 Hematology Analyzer provides automated Reticulocyte analysis and enumeration of nucleated red blood cells (NRBCs) as well as an automated method for enumeration of RBCs and WBCs in body fluids. 1.2 INDICATIONS FOR USE The purpose of the LH 700 Series (Figure 1.
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USE AND FUNCTION INDICATIONS FOR USE Parameters The system determines these hematologic parameters of whole-blood specimens: WBC White Blood Cell or leukocyte count RBC Red Blood Cell or erythrocyte count Hgb Hemoglobin concentration Hct Hematocrit (relative volume of erythrocytes) MCV Mean Corpuscular (erythrocyte) Volume MCH Mean Corpuscular (erythrocyte) Hemoglobin MCHC Mean Corpuscular (erythrocyte) Hemoglobin Concentration RDW Red Cell (erythrocyte volume) Distribution Width Plt Plate
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USE AND FUNCTION QUALITY CONTROL (QC) 1.3 QUALITY CONTROL (QC) Your laboratory can use these QC techniques with the LH 700 Series: r Daily instrument checks r Commercial or Patient Controls r Delta checks r XB Analysis r Extended QC r XM Analysis r Interlaboratory Quality Assurance Program (IQAP) Quality Assurance includes routine maintenance and service in conjunction with the use of controls and calibrators.
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USE AND FUNCTION METHOD HISTORY by size.10, 11 Electronic refinements in the Model S-PLUS enhanced the accuracy of the hydrodynamic method. Von Behrens and Paulus have also cited the feasibility of counting thrombocytes by the Coulter method.12, 13 Corrected WBC Counts White Blood Cell count results from the CBC analysis. The WBC count may be adjusted for interfering substances when appropriate.
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USE AND FUNCTION METHOD HISTORY Conductivity Analysis Cell walls act as conductors to high-frequency current. The current, while passing through the cell walls and through each cell interior, detects differences in the insulating properties of cell components. The current characterizes the nuclear and granular constituents and the chemical composition of the cell interior.
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USE AND FUNCTION METHOD HISTORY NRBC Enumeration The NRBC Enumeration is achieved through the combined use of impedance and VCS technology and a proprietary algorithm. The first step in NRBC enumeration is the identification of particles in the NRBC signature position in the differential data plot. This information is generated from VCS analysis of the cells. Figure 1.
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USE AND FUNCTION METHOD HISTORY dataplot and the WBC histogram are consistent with NRBCs, the NRBC count is derived from the WBC histogram. COULTER IntelliKinetics Application The LH 700 Series utilizes the COULTER IntelliKinetics application. Control of reaction kinetics is extremely important to ensure the best performance of the automated white cell differential and reticulocyte analysis.
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USE AND FUNCTION SYSTEM COMPONENTS Users can enable/disable Extended QC Rules for 5C Cell Control. 1.5 SYSTEM COMPONENTS The LH 700 Series is a modular system that consists of the following units. Power Supply This unit consists of two assemblies. The Electronic Power Supply assembly provides the regulated and unregulated voltages required by the circuitry of the system. The Pneumatic Power Supply assembly is the source of air pressure and vacuum.
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USE AND FUNCTION HARDWARE OPTIONS 1.6 HARDWARE OPTIONS Graphic/Laser Printer You can use any printer that is supported by the Microsoft Windows 2000 operating system. The Printer prints the data displayed on the LH Workstation screen, including parameter data and graphics. LH SlideMaker The LH SlideMaker makes blood smears from samples as they are being analyzed, according to user-defined criteria.
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USE AND FUNCTION REAGENTS Diluent Beckman Coulter diluents, including LH Series diluent and ISOTON 4 diluent, are isotonic electrolyte solutions that: r r r r r Dilute whole-blood samples. Stabilize cell membranes for accurate counting and sizing. Conducts aperture current. Rinse instrument components between analyses. Carry and focus the sample stream in the flow cell to direct the blood cells through the aperture.
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USE AND FUNCTION MATERIAL SAFETY DATA SHEETS (MSDS) r Retic A Retic Stain is a special solution of New Methylene blue dye. The dye precipitates the basophilic RNA network found in the reticulocytes. r Retic B Retic Clearing Solution is a hypotonic acid solution to clear hemoglobin from the cells without removing the precipitated dye. Cleaners LH Series Cleaner and COULTER CLENZ cleaning agent clean and rinse the internal surfaces of the instrument components.
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USE AND FUNCTION MATERIAL SAFETY DATA SHEETS (MSDS) 1-12 PN 4277248DD
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2INSTALLATION 2 2.1 GENERAL CAUTION Possible system damage can occur if you uncrate the instrument, install it or set it up. Keep the instrument in its packaging until your Beckman Coulter Representative uncrates it for installation and setup. Your instrument is tested before it is shipped from the factory. International symbols and special handling instructions printed on the shipping cartons tell the carrier how to handle this electronic instrument. Carefully inspect all cartons when they arrive.
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INSTALLATION SPECIAL REQUIREMENTS: HARDWARE 33 cm (13 in.) 27.9 cm (11 in.) 30 cm (13 in.) 25.4 cm (10 in.) 44.5 cm (17.5 in.) 76.2 cm (30 in.) 68.6 cm (27 in.) 45.7 cm (18 in.) CLEARANCE Figure 2.2 Space for Small Form Factor (SFF)-type computers 38 cm (15 in.) 88.9 cm (35 in.) CLEARANCE 50.2 cm (19.75 in.) CLEARANCE 25.4 cm (10 in.) 64.8 cm (25.5 in.) 30 cm (12 in.) NEC 50.2 cm (19.75 in.) RW 74.9 cm (29.5 in.) DVD disc 64.5 cm (25.4 in.) LH 780 60.3 cm (23.75 in.
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INSTALLATION SPECIAL REQUIREMENTS: HARDWARE Monitor 60 2080 Building outlets must be properly grounded and transients protected. Ambient Temperature and Humidity Operate the system in a room with a temperature of 15.5° to 32°C (60° to 90°F) and humidity up to 95% without condensation. If the average room ambient temperature changes more than 5.5°C (10°F) from the calibrating temperature, verify calibration and recalibrate if necessary to ensure conformance to specifications.
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INSTALLATION INTERUNIT CONNECTIONS 2.3 INTERUNIT CONNECTIONS Power and Signal Cables Figure 2.3 shows the interunit connections of the power and signal cables, highlighting the DB-25 pin connector, if being used. Your Beckman Coulter Representative makes these connections when installing the instrument. Figure 2.
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INSTALLATION INTERUNIT CONNECTIONS Figure 2.4 Rear of instrument with DB 9-Pin Connector Note: The DB 9-pin connector on the computer base, designated as COM 1, is located beside the video display connector. Pneumatic/Hydraulic Tubing Connections CAUTION Possible reagent siphoning effect and priming problems can occur if a reagent container is placed above the level of the Analyzer. Do not place reagent containers above the level of the Analyzer.
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INSTALLATION INTERUNIT CONNECTIONS Figure 2.5 Pneumatic/Hydraulic Connections 1 2 3 4 5 B A 30 PSI WASTE LEVEL SENSOR DILUENT VAC WASTE CBC LYSE RETIC CLEAR RETIC STAIN PACK PRESERVE PACK LYSE Back of diluter 5 PSI CLEANER 2 3 Retic clear Retic stain Diluent Waste Cleaning agent 1 CBC lyse 4 Pak preserve 5 Power supply Pak lyse A 30 PSI 5 PSI VAC B LH Series PAK LH Series RETIC PAK Table 2.
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3OPERATION PRINCIPLES 3 3.1 COULTER METHOD CBC Analysis The Coulter method counts and sizes cells by detecting and measuring changes in electrical resistance when a particle (such as a cell) in a conductive liquid goes through a small aperture. See Figure 3.1. Figure 3.
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OPERATION PRINCIPLES AUTOMATIC ASPIRATION MODE Effect of Reagents The conductive diluent must affect cells minimally, if at all. Both lytic reagents must destroy erythrocytes without significantly affecting leukocytes. They must work rapidly to satisfy the speed with which the system works. The leukocyte preservative must r Provide clear separation of the white blood cell populations, and r Preserve leukocytes in their near-natural state for accurate cytometric measurement.
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OPERATION PRINCIPLES AUTOMATIC ASPIRATION MODE Figure 3.
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OPERATION PRINCIPLES AUTOMATIC ASPIRATION MODE 11. After the last tube in the cassette is aspirated, the cassette continues along the rocker bed and is deposited onto the unloading bay platform. The left platform lifts the cassette into the exit bay, where up to 12 cassettes can be stacked. Aspiration At the sampling station, after the cap is pierced: 1.
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OPERATION PRINCIPLES AUTOMATIC ASPIRATION MODE After aspiration of the blood sample, the sliding plate of the diff segment valve (shear valve) moves, aligning the reagent input and output ports with the diff segment isolated in the stationary plate of the valve. The diff lytic reagent pump dispenses diff lytic reagent, displacing the reagent in the diff heater. The reagent from the diff heater flows through the diff segment valve, diluting the diff blood segment into the mixing chamber.
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OPERATION PRINCIPLES AUTOMATIC ASPIRATION MODE In addition, the instrument activates the blood/stain pump to remove residual stained blood from the lines and activates the retic clearing solution pump to rinse the retic chamber and associated tubing. CBC Sensing System Vacuum, equal to 6 in. of mercury, draws a precise volume of suspension from each bath through the three apertures. At the same time, sweep flow is drawn behind the RBC apertures to prevent cells from re-entering the sensing zone.
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OPERATION PRINCIPLES AUTOMATIC ASPIRATION MODE Figure 3.3 shows the Triple Transducer Module and its protective housing as it resides in the Diluter. WARNING The laser beam can cause damage to your eyes and to the instrument. Do not attempt to remove the laser from the Diluter module. If removal is required, it must be done only by a Beckman Coulter Representative. Tamper-proof screws secure the protective housing; they can only be removed with a special tool.
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OPERATION PRINCIPLES MANUAL ASPIRATION MODE Figure 3.4 Triple Transducer Module with Protective Housing Cut Away LASER ON LAMP LASER RADIATION WHEN OPEN AND INTERLOCK DEFEATED. AVOID DIRECT EYE EXPOSURE. ELECTROMAGNETIC SHIELD LASER RADIATION IS EMITTED FROM THIS APERTURE AVOI D EXPOSURE 1096 Mellon Avenue Manteca, CA 95337 MODEL MANUFACTURED SERIAL NO. FLOW CELL THIS LASER DOES NOT COMPLY WI TH 21 CFR 1040. USE ONLY A S A COMPONENT. SEE INSTALLATION INSTRUCTIONS.
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OPERATION PRINCIPLES COUNTING AND SIZING The Manual mode of operation is like the Automatic mode except: r 3.4 Before you run the sample, you enter the sample identification number by performing any of the following-t Scanning the bar-code label on the tube t Entering the ID number on the Numeric Keypad t Entering the ID number in the bar-code field on the LH Workstation from its keyboard. r You use an open vial and introduce the sample at the aspirator tip.
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OPERATION PRINCIPLES COUNTING AND SIZING Coincidence Correction Occasionally, more than one cell passes through the aperture at the same time. When cells coincide, the Analyzer counts only one pulse. As the frequency of coincidence is proportional to the actual count, the system automatically corrects results for coincidence.
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OPERATION PRINCIPLES COUNTING AND SIZING Figure 3.5 Sweep Flow A B TO WASTE SENSING ZONE CELL CELL SENSING ZONE SWIRLING EFFECT NO SWEEP FLOW SWEEP FLOW RBC Size Distribution After editing, the RED/WHITE PROCESSOR converts the three sets of both the RBC and WBC pulses. It converts each pulse to a number that corresponds to the size of the cell. The Analyzer Microcontroller card (AMC) reads this data and generates histograms.
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OPERATION PRINCIPLES MEASUREMENT OF HEMOGLOBIN CONCENTRATION 3. The mode is between 3 and 15 fL. 4. The @PDW is < 20 5. The height of pulses in channels 0 and 1 is < 1.5 times the height of the mode channel. The PLT is then calculated as the area under the fitted histogram. Special conditions include, for example, the presence of microcytic red cells or red cell fragments that might interfere with counting in the platelet channels.
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OPERATION PRINCIPLES DATAPLOT DEVELOPMENT converted to absorbance, the reagent-blank signal level provides a reference to which the sample signal is compared. 3.6 DATAPLOT DEVELOPMENT At the Workstation, raw data is analyzed to: r Position each cell within a three-dimensional space r Plot the data on a two-dimensional DataPlot r Display and report results.
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OPERATION PRINCIPLES PARAMETERS AND THEIR DERIVATION On the WBC differential DataPlot the axes are: Volume = green RLS (rotated light scatter) = red OP (opacity) = blue On the Retic DataPlot the axes are: Volume = green LLS (linear light scatter) = red OP (opacity) = blue 3.7 PARAMETERS AND THEIR DERIVATION Mathematical expressions in this section are in U.S. units of measurement. Parameter units can be changed to other International System of Units (SI) through Setup on the LH Workstation.
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OPERATION PRINCIPLES PARAMETERS AND THEIR DERIVATION Derived From WBC Histogram Parameter Derived From NRBC% WBC Histogram and VCS Technology Calculated Parameter Hct MCH MCHC DIFF# parameters RET# IRF MRV NRBC# White Blood Cell (WBC) Count WBC is the number of leukocytes measured directly, multiplied by the calibration factor. This number is expressed as WBC = n × 10 3 cells / µL Red Blood Cell (RBC) RBC is the number of erythrocytes measured directly, multiplied by the calibration factor.
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OPERATION PRINCIPLES PARAMETERS AND THEIR DERIVATION Mean Corpuscular Volume (MCV) MCV is the average volume of individual erythrocytes derived from the RBC histogram. The system multiplies the number of RBCs in each channel by the size of the RBCs in that channel. The products of each channel between 36 fL and 360 fL are added. This sum is divided by the total number of RBCs between 36 fL and 360 fL. The analyzer then multiplies by a calibration constant and expresses MCV in femtoliters.
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OPERATION PRINCIPLES PARAMETERS AND THEIR DERIVATION NRBC # (Nucleated Red Blood Cells) A parameter that is calculated from the NRBC % and the total WBC count. NRBC # represents the total number of nucleated Red Blood Cells. NRBC (103 cells µL) = NRBC% / 100 x WBC count Differential (Diff) Diff Percentages (DIFF%) The percentages of leukocytes from each category are derived from the DataPlot. NE% = no. of cells inside NE area × 100 no. of cells inside NE + LY + MO + EO + BA LY% = no.
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OPERATION PRINCIPLES PARAMETERS AND THEIR DERIVATION Reticulocyte (Retic) Parameters Retic Percent (RET%) Number of reticulocytes per 100 RBCs. This parameter is directly measured and reported as %, a percentage of RBCs.
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OPERATION PRINCIPLES XB ANALYSIS 3.8 XB ANALYSIS Studies (Bull 1974, Koepke 1981) indicate that the red cell indices (MCV, MCH, and MCHC) of patient populations are stable over time.39,40 This stability characteristic of the indices is the basis of a quality-control technique called XB Analysis. In a manually implemented system, population means (target values) are established by analyzing as large a sample as possible, at least 250, but ideally 1,000 blood samples.
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OPERATION PRINCIPLES XB ANALYSIS The LH Workstation calculates and displays the percent difference between each batch mean and its corresponding preset target value.
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OPERATION PRINCIPLES XB ANALYSIS Table 3.1 Effect of Directly-Measured Parameters on the Red Cell Indices Index Directly-Measured Parameter MCV RBC HGB Increased Decreased Increased Decreased Increased Decreased MCV HIGH LOW NORMAL NORMAL NORMAL NORMAL MCH NORMAL NORMAL LOW HIGH HIGH LOW MCHC LOW HIGH LOW HIGH HIGH LOW See the Glossary for terms used with the XB Analysis.
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OPERATION PRINCIPLES XB ANALYSIS 3-22 PN 4277248DD
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4SPECIFICATIONS/CHARACTERISTICS 4 4.1 PHYSICAL SPECIFICATIONS Dimensions Unit Height* Width* Depth* Weight Analyzer/Diluter 88.9 cm 101.6 cm 61 cm 93.2 k (35 in.) (40 in.) (24 in.) (205 lb) 59 cm 35.5 cm 60 cm 56.7 k (23.3 in.) (14 in.) (24 in.) (125 lb) Power Supply * ±5.1 cm (2 in.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE SPECIFICATIONS--LH 700 Series Table 4.1 Within-Run Precision Parameter @ Approximate Level Limit WBC 9 to 11 x 103 cells/µL ≤1.7% CV RBC 4.5 to 5.5 x 106 cells/µL ≤0.8% CV Hgb 14 to 16 g/dL ≤0.8% CV MCV 80 to 90 fL ≤0.8% CV RDW 12 to 14% ≤2.2% CV Plt 280 to 320 x 103 cells/µL ≤3.3% CV Plt 90 to 110 x 103 cells/µL ≤6.6% CV Plt 10.0 to 15.0 x 103 cells/µL ≤14.0% CV MPV 8 to 10 fL ≤2.2% CV NE% 50 to 60% 2SD ≤3.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE SPECIFICATIONS--LH 700 Series Table 4.2 Accuracy, CBC Hgb ±0.2 ±3.0% MCV* N/A ±2.0% RDW ±0.5 ±5.0% Plt ±10 ±7.0% MPV N/A ±5.0% *Due to the effect of temperature on red cell size, the specification applies to a temperature range of 70 to 80 degrees F. Accuracy, WBC Differential Accuracy of the WBC differential should be determined by comparison against either or both of two comparator methods.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE SPECIFICATIONS--LH 700 Series Table 4.5 Linearity Limits Parameter Linearity Range Limits (mean difference or % difference, whichever is greater) WBC x 103 cells/µL 0.00 to 100.0 100.1 to 400.0 ±0.2 or 3.0% ±9% RBC x 106 cells/µL 0.00 to 8.00 ±0.05 or 2.0% Hgb g/dL 0.0 to 25.0 ±0.2 or 3.0% Plt x 103 cells/µL 0 to 3000 ±10 or 7.0% Background Background counts for CBC parameters are performed during the Startup cycle.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE CHARACTERISTICS replaced by pluses (+++++). The reportable range identifies the values where the instrument is accurate, and reflects the range studied in accuracy testing. See Table 4.6. Table 4.6 Operating and Reportable Ranges Parameter Operating Range Reportable Range WBC 0.00 to 900.00 x 103 cells/µL 0.00 to 400.00 x 103 cells/µL RBC 0.00 to 20.00 x 106 cells/µL 0.00 to 8.00 x 106 cells/µL Hgb 0.0 to 99.9 g/dL 0.0 to 25.0 g/dL Hct 0.0 to 99.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE CHARACTERISTICS Sample Stability The following tables show the average results for specimens from five normal donors collected in K3EDTA‡. The specimens were stored at room temperature and cold temperature. For this study, room temperature was 72 - 73º (22 - 23ºC) and cold temperature was 37 - 39ºF (3 - 4ºC). Room temperature specimens were mixed for 22 inversions and then immediately analyzed.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE CHARACTERISTICS Table 4.10 DIFF% Sample Stability, Cold Temperature‡ Hours NE LY MO EO BA 8 56.63 34.92 6.32 1.76 0.37 16 58.08 34.96 5.05 1.65 0.27 24 58.71 33.57 5.80 1.63 0.29 36 60.58 31.41 6.33 1.47 0.22 48 61.00 31.14 6.43 1.11 0.32 *Results analyzed @ Room Temperature Table 4.11 DIFF# Sample Stability, Room Temperature‡ Hours NE LY MO EO BA 1 5.45 2.32 0.54 0.11 0.03 4 5.60 2.45 0.55 0.12 0.03 8 5.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE CHARACTERISTICS Table 4.14 RETIC Sample Stability, Cold Temperature‡ Hours RETIC% RETIC# MRV IRV 1* 1.771 0.078 103.87 0.356 4 1.900 0.084 103.92 0.354 8 1.874 0.083 103.94 0.347 16 1.957 0.086 105.48 0.357 24 1.907 0.084 104.63 0.360 36 1.670 0.074 106.86 0.360 48 1.644 0.073 104.70 0.345 60 1.674 0.074 106.08 0.374 72 1.786 0.079 105.84 0.368 *Results analyzed @ Room Temperature Table 4.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE CHARACTERISTICS Table 4.17 Differential Suspect Flagging at Room Temperature Sample # 1 hour 4 hour 8 hour 16 hour 24 hour 1 Imm Ne 1 Imm Ne 1 Imm Ne 1 Imm Ne 1 Mo Blast, Imm Ne 1, Imm Ne 2 2 * * * * Imm Ne 2 3 * * * * * 4 * * * * * 5 * * * * * *Indicates that there were no flags present. . Table 4.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE CHARACTERISTICS NRBC Accuracy Characteristics NRBC Accuracy Characteristics was defined as the agreement between the LH 700 Series and the results given by the reference method (NCCLS H20A) and a predicate hematology analyzer. All NRBC % results > 2 and < 15 will be flagged with an R symbol. Beckman Coulter, Inc. recommends a slide review as per your individual laboratory protocol.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE CHARACTERISTICS Table 4.23 Normal Population Study (Continued) RDW % 13.30 12.09 15.19 Plt x 103 cells/µL 248.65 169.06 397.10 MPV fL 9.31 7.54 11.24 NE % 59.78 41.71 73.74 LY % 28.67 18.39 44.42 MO % 7.89 4.69 12.66 EO % 2.96 0.75 8.06 BA % 0.62 0.17 1.37 NE x 103 cells/µL 3.74 1.47 7.06 LY x 103 cells/µL 1.84 0.65 4.25 MO x 103 cells/µL 0.50 0.17 1.21 EO x 103 cells/µL 0.20 0.03 0.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE CHARACTERISTICS Sample Type Anticoagulated human whole blood Recommended Anticoagulant K2EDTA or K3EDTA Sampling Modes Aspiration Automatic cap-piercing, whole blood Manual, open vial, whole blood and diluted specimens. Test/Cycle CBC CBC/DIFF CBC/RETIC CBC/DIFF/RETIC RETIC Throughput, Automatic Mode Typical throughput performance is described for samples with elevated parameter levels.
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SPECIFICATIONS/CHARACTERISTICS PERFORMANCE CHARACTERISTICS Pneumatic Supplies (Internally Regulated) Pressure = 60 psi (pounds per square inch) Vacuum = 22 in. Hg (inches of mercury) minimum at sea level Calibration Stability Variation with temperature: If ambient room temperature changes by less than 5.5ºC (10ºF) from the calibrating temperature, and the temperature is within the temperature specifications, then the LH 700 Series does not require calibration.
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE SYMBOLOGY OVERVIEW RBC Very high WBC count, high concentration of very large platelets, auto-agglutination. If hemolysis is occurring in vivo, the instrument RBC may be flagged as low, reflecting the true circulating cells. If, however, the hemolysis is in vitro, the specimen may give falsely low RBC results. Cell counts due to in vitro hemolysis do not represent the number of circulating red blood cells.
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE SYMBOLOGY OVERVIEW A symbology is defined by the following characteristics: Character Set This refers to the range of data characters that can be encoded for a specific symbology. There are essentially three types: r Numeric—The symbology can encode only numeric characters. r Alphanumeric—Can encode the full alpha and numeric characters of the ASCII character set. r 128—Can encode all the 128 available ASCII characters.
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SPECIFICATIONS/CHARACTERISTICS BAR CODES AND THE LH 700 Series 4.5 BAR CODES AND THE LH 700 Series IMPORTANT 1. DO NOT use the following characters # @ [ \ ] ` { | } ~ in Specimen or Patient identifiers. There is a potential for Specimen or Patient misidentification to occur. The system will substitute or omit these characters when the system is configured in a language other than English or Chinese. 2. Risk of misidentification.
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SPECIFICATIONS/CHARACTERISTICS BAR CODES AND THE LH 700 Series Codabar Codabar has a character set of 16 characters. It is a discrete, self-checking symbology and is most commonly used in libraries and blood banks. NW-7 NW-7 is similar to Codabar. It uses numeric characters only and a different checksum algorithm. Code 128/USS 128 Code 128 is a continuous, variable length symbology. This symbology has 106 different printed characters. Code 128 is character dependent.
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS 4.6 BAR-CODE LABEL SPECIFICATIONS IMPORTANT 1. DO NOT use the following characters # @ [ \ ] ` { | } ~ in Specimen or Patient identifiers. There is a potential for Specimen or Patient misidentification to occur. The system will substitute or omit these characters when the system in configured in a language other than English or Chinese. 2. Risk of misidentification.
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS Label Dimensions and Data The dimensional and data specifications are illustrated in Figure 4.1, Bar-Code Label Specifications. Table 4.24, Bar-Code Label Specifications, explains the specifications called out in Figure 4.1, Bar-Code Label Specifications. Figure 4.1 Bar-Code Label Specifications LEADING EDGE OF LABEL FIRST BAR LINE (SEE SPEC. 1) A B 0.045 +0.045" 0.055" ALL SUBSEQUENT BAR LINES (SEE SPEC. 2) HUMAN-READABLE CODE (SEE SPEC.
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS Acceptable Bar Codes Within the given specifications, the scanner automatically distinguishes the following bar codes. Interleaved 2-of-5 Code 39 Codabar NW7 Code 128/USS 128 Extended Digit Bar Code When using bar-coded data lengths from 12 to 16 data characters, consider these recommendations: r Use a tube that can accommodate a label length between 53.34 mm (2.1 in.) and 63.50 mm (2.5 in.) for 16-character bar codes.
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS Table 4.
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS 6. This number is the value of the checksum character. 7. Determine whether total number of characters (message plus checksum) is odd or even. If odd, add a leading nonsignificant zero to the message to produce an even number of characters as required by the symbology.
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS Examples: 1. MESSAGE VALUE 2 2 3 3 4 4 7 7 1 1 3 3 2+3+4+7+1+3 = 20 20 ÷ 16 = 1, REMAINDER 4 The value 4 corresponds to character 4; therefore, the checksum is 4 and the final decoded message is 2347134. 2.
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS Checksum Technique: r The start and stop digits are not used as part of the checksum calculation. r The Checksum digit immediately precedes the stop digit. r Weighted modulus 11 calculation method.
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS Code 39 Bar Code This code uses three to 16 data digits with checksum enabled. Use of a 3 digit bar code without checksum enabled is not allowed. The value assigned to each of the characters is: Table 4.28 Code 39 Character Value Table: CHARACTER VALUE CHARACTER VALUE CHARACTER VALUE 0 0 F 15 U 30 1 1 G 16 V 31 2 2 H 17 W 32 3 3 I 18 X 33 4 4 J 19 Y 34 5 5 K 20 Z 35 6 6 L 21 - 36 7 7 M 22 .
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS Code 128 This code uses four to 16 data digits on the LH 700 Series and four to 11 data digits for the SlideMaker bar-code label printing. The checksum character immediately precedes the stop character. The checksum character used with Code 128 must conform to the AIM USS 128 checksum generation procedure.
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS PN 4277248DD VALUE CODE A CODE B CODE C 9 ) ) 09 10 * * 10 11 + + 11 12 , , 12 13 - - 13 14 . .
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS 4-28 VALUE CODE A CODE B CODE C 45 M M 45 46 N N 46 47 O O 47 48 P P 48 49 Q Q 49 50 R R 50 51 S S 51 52 T T 52 53 U U 53 54 V V 54 55 W W 55 56 X X 56 57 Y Y 57 58 Z Z 58 59 [ [ 59 60 \ \ 60 61 ] ] 61 62 ^ ^ 62 63 ___ ___ 63 64 NUL ` 64 65 SOH a 65 66 STX b 66 67 ETX c 67 68 EOT d 68 69 ENQ e 69 70 ACK f 70 71 BEL g 71 72 BS h 72
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS PN 4277248DD VALUE CODE A CODE B CODE C 81 DC1 q 81 82 DC2 r 82 83 DC3 s 83 84 DC4 t 84 85 NAK u 85 86 SYN v 86 87 ETB w 87 88 CAN x 88 89 EM y 89 90 SUB z 90 91 ESC { 91 92 FS | 92 93 GS } 93 94 RS ~ 94 95 US DEL 95 96 FNC 3 FNC 3 96 97 FNC 2 FNC 2 97 98 SHIFT SHIFT 98 99 CODE C CODE C 99 100 CODE B FNC 4 CODE B (A) 101 FNC 4 CODE A CODE A (A) 102 FNC 1 FN
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SPECIFICATIONS/CHARACTERISTICS BAR-CODE LABEL SPECIFICATIONS 4-30 PN 4277248DD
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5HAZARDS 5 5.1 LASER SAFETY WARNING Possible harm to operator. Do not use any controls, make any adjustments, or perform any procedures other than those specified herein. To do so may result in hazardous radiation exposure. The Triple Transducer Module and Bar-Code Reader contain lasers. A laser is a unique light source that exhibits characteristics different from conventional light sources.
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HAZARDS LASER WARNING LABELS Figure 5.1 Laser Warning Label, Protective Housing Cut Away LASER ON LAMP LASER RADIATION WHEN OPEN AND INTERLOCK DEFEATED. AVOID DIRECT EYE EXPOSURE. ELECTROMAGNETIC SHIELD LASER RADIATION IS EMITTED FROM THIS APERTURE AVOI D EXPOSURE 1096 Mellon Avenue Manteca, CA 95337 MODEL MANUFACTURED SERIAL NO. FLOW CELL THIS LASER DOES NOT COMPLY WI TH 21 CFR 1040. USE ONLY A S A COMPONENT. SEE INSTALLATION INSTRUCTIONS.
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HAZARDS BAR-CODE READER Figure 5.2 Laser Warning Label Locations, Protective Housing On Located on back of unit THIS LASER PRODUCT CONFORMS TO THE PROVISIONS OF 21 CFR. SUBCHAPTER J. SECTIONS 1040. 10 AND 1040. 11 CLASS 1 LASER PRODUCT Manufactured: DATE BY: BECKMAN COULTER, INC. 250 S. KRAEMER BLVD. BREA, CA 92821 2427796 LASER RADATION WHEN OPEN AND INTERLOCK DEFEATED. AVOID DIRECT EYE EXPOSURE. I II III IV V VI VII VIII 5.4 BAR-CODE READER Figure 5.
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HAZARDS BAR-CODE READER Figure 5.4 Bar-Code Reader Laser Warning Label Location, Protective Housing On L a s e r r a d ia tio n e m is s io n in d ic a to r ( L E D ) C A U T IO N *S Y S T E M L a s e r r a d ia tio n w h e n o p e n a n d in te r lo c k d e fe a te d . D O N O T S T A R E IN T O B E A M .
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HAZARDS HANDHELD SCANNER 5.5 HANDHELD SCANNER Figure 5.5 illustrates the label on the PSC handheld scanner. Figure 5.5 Handheld Scanner 5.6 DISPOSAL OF ELECTRICAL INSTRUMENTATION It is very important that customers understand and follow all laws regarding the safe and proper disposal of electrical instrumentation. A28219-AA The symbol of a crossed-out wheeled bin on the product is required in accordance with the Waste Electrical and Electronic Equipment (WEEE) Directive of the European Union.
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HAZARDS DISPOSAL OF ELECTRICAL INSTRUMENTATION 5-6 PN 4277248DD
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REFERENCES 1. Coulter WH. High speed automatic blood cell counter and cell size analyzer. Paper presented at National Electronics Conference, Chicago, IL, 1956; October 3. 2. Brecher G, Schneiderman M and Williams GZ. Evaluation of electronic red blood cell counter. Am J Clin Path, 1956; 26:1439-1449. 3. Brittin GM, Brecher G and Johnson CA. Evaluation of the COULTER COUNTER Model S. Am J Clin Path, 1969; 52(6):679-689. 4. Gottman AW.
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REFERENCES 20. Hoffman RA and Britt WB. Flow-system measurement of cell impedance properties. J Histochem Cytochem, 1979; 27(1):234-240. 21. Leif RC, Scwartz S, Rodriguez CM, Peel-Fernandez L, Groves M, Leif SB, Cayer M and Crews H. Two-dimensional impedance studies of BSA buoyant density separated human erythrocytes. Cytometry, 1985; 6(1):13-21. 22. Coulter WH et al.
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REFERENCES 38. Kachel V and Ruhenstroth-Bauer G. Methodik and Ergebissne Optiseher Formfatorunter-suchungen bei der Zellvolumenmessung nach Coulter. Micros Acta, 1976; 75:419-423. 39. Bull BS, and Elashoff RM et al.: 1974. A study of various estimators for the derivation of quality control procedures from patient erythocytic indices. Am J Clin Path 61(4):475. 40. Koepke JA. Tips on technology. MLO:15, 1981. 41. NCCLS document H20-A. Approved Standard for the Evaluation of the Differential Cell Count.
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REFERENCES 54. Bancroft A. J., Abel E. W. , Mclaren M. , Belch J. J. F., Mean platelet volume is a useful parameter: a reproducible routine method using a modified Coulter Thrombocytometer, Platelets 11:379–387, 2005. *National Committee for Clinical Laboratory Standards has changed to Clinical and Laboratory Standards Institute (CLSI).
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GLOSSARY µL Microliter, a unit of volumetric measurement equal to one millionth of a liter. µm Micrometer, a unit of linear measurement equal to one millionth of a meter. A Ampere, a unit of electric current. Accuracy Ability of the instrument to agree with a predetermined reference value. Action Limits Values established by your laboratory to flag results requiring action.
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GLOSSARY AutoCollation A Workstation feature that automatically combines results of different test modes (CBC/Diff and Retic) analyzed from a sample with an identical patient or sample ID. The different test modes must be performed within a predetermined time. Automatic Aspiration The closed-vial method of running a sample. You place a closed tube sample in Mode a cassette and place the cassette on the LH 700 Series loading bay.
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GLOSSARY Batch Mean The mean or average of a set of samples. For Xb and XM Analysis, the batch mean is a value based on a statistical averaging technique and is a type of "weighted moving average." It is used to estimate what a simple average result of a very large number of samples (population mean) might be by using a small number of samples. Batch Print Job Selecting multiple items from the database, then selecting the print icon to print all of them at the same time.
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GLOSSARY CLSI Clinical and Laboratory Standard Institute formerly known as the National Committee for Clinical Laboratory Standards (NCCLS). cm Centimeter, a unit of linear measurement. Codes On windows and printouts, symbols, such as -----, ++++, :::::, appear with or in place of sample or control results because of irregularities. A code can only be generated by the instrument. Coefficient of Variation An expression, in percent, of data spread as related to the mean.
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GLOSSARY Definitive Messages Definitive messages appear in a separate area of the screen display, printout, and host transmission. Definitive messages such as Anisocytosis, Leukopenia, etc, are laboratory-defined. Definitive messages are defined based on numeric limits generated by your laboratory. If results exceed the limits, the Workstation generates a message. Results that generate these messages may require review. Check your laboratory’s protocol for handling the particular message.
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GLOSSARY Extended QC Random Error Random error is Extended QC’s measurement of imprecision. Extended QC Systematic Error Systematic error is Extended QC’s measurement of bias. A systematic error is defined as the deviation of the mean from the target value. Extended QC - Total Error Total Error is Extended QC’s measurement of inaccuracy, as compared to an established limit. Total Error is defined as the deviation of a single measurement from the Target Value that was setup for your 5C Cell control (e.
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GLOSSARY Histograms Graphic representations of cell frequency vs. size. Histograms provide information about leukocyte, erythrocyte and thrombocyte frequency and their distribution about the mean. They also might show the presence of subpopulations. Hz Hertz, a unit of frequency. i.d. Inside diameter. IEC International Electrtechnical Commission. Information System Any host or laboratory computer system. Instrument The Analyzer and Diluter portion of the LH 700 Series.
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GLOSSARY LY % (Lymphocytes) A WBC differential parameter result from the Diff sample analysis. The percent (%) is measured directly using VCS technology. LY % = no. of cells inside LY area x 100 no. of cells inside NE+LY+MO+EO+BA m Meter, a unit of linear measurement. Manual aspiration mode The open-vial method of running a sample. Also called: open-vial mode, secondary mode, manual sampling mode.
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GLOSSARY Modes (Test) Use Default Type on the Command Center to specify the test mode. It determines how the LH 700 Series processes data. Mode-to-Mode Matching Agreement between patient results in Automatic aspiration mode and Manual aspiration mode. MPV (Mean Platelet Volume) MPV is the mean cell volume for platelets. The system derives this parameter from the Plt histogram. MRV (Mean Reticulocyte Volume) Mean volume of the Retic population. This parameter is derived from VCS technology.
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GLOSSARY Operator ID A 16-character identifier that uniquely identifies the processor of the samples. If you forget your user name or password, contact your lab administrator. The user name works with your password to uniquely identify you to the system. Also called: operator ID. Open-vial Mode The open-vial method of running a sample. You immerse the aspirator tip in the sample. The instrument automatically aspirates the sample.
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GLOSSARY Pending List A feature of the Workstation that lets you view a list of samples the instrument has not yet processed. You can view a list of all the unprocessed samples or a list of unprocessed samples for a particular test mode. You can sort these lists by any column you choose. Also called: Worklist, Pending List, Work Order. Pericardial Fluid Fluid taken from the chest cavity around the heart. Peritoneal Fluid Fluid taken from the abdominal cavity.
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GLOSSARY Predilute Diltion of a sample prior to analysis on the analyzer. The Predilute mode on the LH 700 Series may be used to dilute specimens that exceed the reportable range, or to run a citrated tube when clumped platelets are suspected. The Predilute mode runs in the CBC test mode via manual aspiration only. You may enter dilution factors from 1.1 to 5.0. The sample results are automatically multiplied by the dilution factor entered.
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GLOSSARY Reportable range This range represents the clinical limits of values that have been tested and found to be accurate, precise, and linear. Reportable range can be the same as the linear range, but it is usually a subset of the linear range. Reproducibility A procedure to check that the system gives consistent results (within established limits) every time it measures the same sample. Also called: precision. Reset button, Analyzer r Resets the electronic and pneumatic subsystems.
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GLOSSARY SD (Standard Deviation) A measure of deviation from the mean. SD = Secondary Mode Σ( x − x)2 n−1 The open-vial method of running a sample. You immerse the aspirator tip in the sample. The instrument automatically aspirates the sample. If the instrument fails to aspirate automatically, you can press and release the activator to aspirate the sample. Also called: open-vial mode, secondary mode, manual sampling mode. Select When an item is selected, a visual cue is present. For example, field.
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GLOSSARY Synovial Fluid Joint fluid. Test (Modes) The LH 700 Series has five different test modes. The test mode determines how the LH 700 Series processes data. For example, in the CBC test mode the LH 700 Series performs a basic complete blood count on whole-blood samples. The test mode appears as a field on the QA Results & Graphics window. ToDo List A feature of the Workstation that lets you view a list of samples the instrument has not yet processed.
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GLOSSARY WBC (White Blood Cell) White Blood Cell count results from the CBC analysis. The WBC count is adjusted for interfering substances when appropriate. No further correction of WBC is required. Interfering substances include, but are not limited to NRBC, giant platelets, platelet clumps, unlysed RBCs and RBC fragments. This parameter is measured directly using the Coulter Principle. WBC = n x 10 3 cells/µL Wettability The ability of any solid surface to be wetted when in contact with a liquid.
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INDEX A A (definition), GLOSSARY--1 accessibility, 2--1 Accuracy (definition), GLOSSARY--1 accuracy, 4--1 CBC, 4--1 of reticulocyte parameters, 4--1 qualification, 4--1 reticulocyte, 4--1 specifications, 4--1 WBC differential, 4--1 Action Limits (definition), GLOSSARY--1 Active Set (definition), GLOSSARY--1 Algorithm (definition), GLOSSARY--1 Analytical Station (definition), GLOSSARY--1 Analyzer, GLOSSARY--1 Analyzer (definition), GLOSSARY--1 Analyzer Pulse (definition), GLOSSARY--1 ANSI (definition), GLOSS
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INDEX sample stability, 4--1 CDC (definition), GLOSSARY--3 CEE (definition), GLOSSARY--3 Cell Control (definition), GLOSSARY--3 characteristics, 4--18 performance, 4--18 Characters (definition), GLOSSARY--3 Check Valve (definition), GLOSSARY--3 checksum algorithm, 4--18 Codabar, 4--18 Code 128, 4--18 Code 39, 4--18 Interleaved 2-of-5, 4--18 Japan Red Cross NW7 Decoding, 4--18 NW7, 4--18 CLIA (definition), GLOSSARY--3 cm (definition), GLOSSARY--4 Codabar bar code, 4--18 Code 128 bar code, 4--18 Code 39 bar
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INDEX H handheld scanner, 4--14, 4--16 bar codes overview, 4--14 handheld scanner,bar codes overview, 4--16 HCT, GLOSSARY--6 HCT (Hematocrit) Field, GLOSSARY--6 hematocrit, GLOSSARY--6 hemoglobin, GLOSSARY--6, GLOSSARY--8 Hemoglobinometry (definition), GLOSSARY--6 Hgb, GLOSSARY--6 HGB (Hemoglobin) Field, GLOSSARY--6 Hgb Voltages Fields, GLOSSARY--6 Histogram (definition), GLOSSARY--7 histogram, GLOSSARY--11, GLOSSARY--12 Hz (definition), GLOSSARY--7 I i.d.
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INDEX monocytes, GLOSSARY--8 MPV (Mean Platelet Volume), GLOSSARY--9 MRV, GLOSSARY--9 MSCV, GLOSSARY--9 mW (definition), GLOSSARY--9 N n (definition), GLOSSARY--9 name, GLOSSARY--6, GLOSSARY--7 NCCLS (definition), GLOSSARY--4, GLOSSARY--9 NE, GLOSSARY--9 NE Number (Neutrophils), GLOSSARY--9 NE Percent (Neutrophils), GLOSSARY--9 NEMA (definition), GLOSSARY--9 neutrophils, GLOSSARY--9 nm (definition), GLOSSARY--9 Nonhematopoietic Cells, GLOSSARY--9 NRBC Count Field, GLOSSARY--9 NRBC Percent Field, GLOSSARY-
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INDEX RBC indices,MCV, GLOSSARY--8 RDW, GLOSSARY--12 RDW (Red Cell Distribution Width) Field, GLOSSARY--12 read, GLOSSARY--6 Hgb voltages fields, GLOSSARY--6 Record (definition), GLOSSARY--12 red blood cell, GLOSSARY--12 red cell distribution width, GLOSSARY--12 red cell indices, GLOSSARY--8 MCH, GLOSSARY--8 MCHC, GLOSSARY--8 MCV, GLOSSARY--8 red cell volume, GLOSSARY--8 Reflex Manager List (definition), GLOSSARY--12 Report Profile (definition), GLOSSARY--12 reportable range (definition), GLOSSARY--13 desc
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INDEX X XB Analysis (definition), GLOSSARY--16 XM Analysis (definition), GLOSSARY--16 6-INDEX PN 4277248DD
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BECKMAN COULTER, INC. CUSTOMER END USER LICENSE AGREEMENT This Product contains software that is owned by Beckman Coulter, Inc. or its suppliers and is protected by United States and international copyright laws and international trade provisions. You must treat the software contained in this Product like any other copyrighted material. This license and your right to use the Product terminate automatically if you violate any part of this agreement. This is a license agreement and not an agreement for sale.
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TRADEMARKS Beckman Coulter, the stylized logo, and COULTER are trademarks of Beckman Coulter, Inc. and are registered with the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders. Find us on the World Wide Web at: www.beckmancoulter.
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COULTER LH 700 SERIES SYSTEM HARD-COPY DOCUMENTATION s Host Transmission PN 4277303 Specifications for transmitting to a host computer. Available in hard copy by request. s Reference PN 4277248 Use and Function • Installation • Operation Principles • Specifications/Characteristics • Precautions/Hazards • References • Glossary • Index Available in hard copy by request.