Product Manual
Table Of Contents
- COULTER LH 750 System
- Reference
- WARNINGS AND PRECAUTIONS
- WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:
- WARNING Risk of operator injury if:
- r All doors, covers and panels are not closed and secured in place prior to and during instrument...
- r The integrity of safety interlocks and sensors is compromised.
- r Instrument alarms and error messages are not acknowledged and acted upon.
- r You contact moving parts.
- r You mishandle broken parts.
- r Doors, covers and panels are not opened, closed, removed and/or replaced with care.
- r Improper tools are used for troubleshooting.
- r Keep doors, covers and panels closed and secured in place while the instrument is in use.
- r Take full advantage of the safety features of the instrument. Do not defeat safety interlocks a...
- r Acknowledge and act upon instrument alarms and error messages.
- r Keep away from moving parts.
- r Report any broken parts to your Beckman Coulter Representative.
- r Open/remove and close/replace doors, covers and panels with care.
- r Use the proper tools when troubleshooting.
- CAUTION System integrity might be compromised and operational failures might occur if:
- r This equipment is used in a manner other than specified. Operate the instrument as instructed i...
- r You introduce software that is not authorized by Beckman Coulter into your computer. Only opera...
- r You install software that is not an original copyrighted version. Only use software that is an ...
- IMPORTANT If you purchased this product from anyone other than Beckman Coulter or an authorized B...
- WARNINGS AND PRECAUTIONS
- REVISION STATUS
- Issue A, 10/01 Software version 1A. Converted from Help Version 1A.012431.
- Issue B, Complete Revision, 5/02 Software version 2A. Converted from Help Version 2A.021501.
- Issue C, 10/03 Software version 2B. Converted from Help Version 2B.031971. Changes were made to:
- Issue D, 04/07 Software version 2D. Converted from Help Version 2D.071161. The name of the manual...
- Issue DA, 08/09 Software version 2D1. Converted from Help Version 2D1.091732.
- Issue DB, 11/10 Software Version 2D1.
- Updates were made to the company corporate address.
- Issue DC, 12/10 Software Version 2D1. Changes were made to:
- Note: Changes that are part of the most recent revision are indicated in text by a bar in the mar...
- This document applies to the latest software listed and higher versions. When a subsequent softwa...
- Issue DD, 01/13 Software Version 2D3. Manual derived from Online Help version 2D3. Changes were m...
- Note: Changes that are part of the most recent revision are indicated in text by a bar in the mar...
- This document applies to the latest software listed and higher versions. When a subsequent softwa...
- CONTENTS
- INTRODUCTION
- This introductory section contains the following topics:
- HOW TO USE YOUR COULTER LH 750 SERIES SYSTEM HARD-COPY MANUALS
- Use the Getting Started booklet to see an overview of the system hardware and software. This docu...
- Use the Reference manual for in-depth information about what the instrument does, the methods it ...
- Use the Special Procedures and Troubleshooting manual to run calibration; to clean, replace or ad...
- Use the Operator’s Guide for the day-to-day operation of your instrument. This document is made u...
- Use the SlideMaker Operator’s Guide for in-depth information about what the SlideMaker does, the ...
- Use the SlideStainer Operator’s Guide for the day-to-day operating and troubleshooting of your Sl...
- Use the Master Index to easily locate a subject in your hard-copy Reference manual, Operator’s Gu...
- Use the Host Transmission Specification to find the information needed to program the transmissio...
- See the Documentation page on the back cover of this manual for the contents of each manual. It c...
- ABOUT THIS MANUAL
- Your LH 750 System Reference Guide is a source of information for the day-to-day operation of you...
- s Chapter 1, Use and Function Contains the intended use of the instrument, a brief history of the...
- s Chapter 2, Installation Contains the instrument requirements, and the diagrams of the reagent/p...
- s Chapter 3, Operation Principles Contains descriptions of the Coulter Method, the normal sample ...
- s Chapter 4, Specifications/Characteristics Details the instrument and performance specifications...
- s Chapter 5, Hazards Describes laser safety precautions and the location of the laser-related lab...
- s References
- s Glossary
- s Index, hard copy only
- Your LH 750 System Reference Guide is a source of information for the day-to-day operation of you...
- ONLINE HELP SYSTEM
- CONVENTIONS
- USE AND FUNCTION
- 1.1 INTENDED USE
- 1.2 INDICATIONS FOR USE
- 1.3 QUALITY CONTROL (QC)
- 1.4 METHOD HISTORY
- Development
- W.H. Coulter (1956) describes the Coulter Principle:1
- This substantial improvement in precision over previous methods helped to establish the erythrocy...
- The COULTER COUNTER Model S analyzer was the first instrument that automated simultaneous multipa...
- Refinements of the COULTER COUNTER analyzer to provide accurate size (volume) distribution data l...
- Among the advantages offered by the Coulter method of counting and sizing was the ability to deri...
- Bull et al. described the use of a COULTER COUNTER analyzer for counting thrombocytes.9 This meth...
- Corrected WBC Counts
- Hemoglobinometry
- Differential Measurement
- Reticulocyte (Retic) Analysis
- Reticulocytes are immature, nonnucleated erythrocytes retaining a small network of basophilic org...
- The most common means of measuring reticulocytes is to use supravital dyes, such as New Methylene...
- Reticulocyte immaturity is related to cell volume and light scatter. Since more immature reticulo...
- Figure 1.3 illustrates the IRF and MRV algorithms. This figure is a representation of the VCS dat...
- The RET% is calculated as the ratio of reticulocytes to the total number of red cells. The spectr...
- NRBC Enumeration
- The NRBC Enumeration is achieved through the combined use of impedance and VCS technology and a p...
- The first step in NRBC enumeration is the identification of particles in the NRBC signature posit...
- Once particles have been identified in this region, the LH 700 Series examines the far left regio...
- If the VCS dataplot and the WBC histogram both indicate the presence of NRBCs, then the combined ...
- COULTER IntelliKinetics Application
- The LH 700 Series utilizes the COULTER IntelliKinetics application. Control of reaction kinetics ...
- The IntelliKinetics application management ensures consistent reaction kinetics. This application...
- The LH 700 Series with the IntelliKinetics application shows improved separation of populations, ...
- XB Analysis
- XM Analysis
- Extended QC
- Development
- 1.5 SYSTEM COMPONENTS
- The LH 700 Series is a modular system that consists of the following units.
- Power Supply
- Diluter
- Analyzer
- LH Workstation
- The LH Workstation holds the data algorithms used to process the List Mode Data supplied by the A...
- The LH Workstation is equipped with a mouse that allows operator interaction with the software.
- Operate the instrument as instructed in your product documentation. Only operate your system’s co...
- The LH Workstation is not for use as a general purpose personal computer.
- Handheld Scanner
- 1.6 HARDWARE OPTIONS
- 1.7 CONTROLS AND CALIBRATOR
- Controls
- Use stable reference controls to monitor the instrument performance as part of your quality contr...
- COULTER 5C Cell control monitors the CBC and differential (Diff) parameters.
- LATRON primer prepares the tubing and instrument components for the LATRON control.
- LATRON control monitors the performance of the volume, conductivity and light scatter measurements.
- Retic-C cell control monitors the reticulocyte (Retic) parameters.
- Lin-C linearity control identifies the reportable range of the instrument's CBC parameters
- Calibrator
- Controls
- 1.8 REAGENTS
- Beckman Coulter developed and tested this Beckman Coulter instrument exclusively for use with Bec...
- Diluent
- CBC Lytic Reagent
- LH Series PAK Reagent System
- The LH Series RETIC PAK Reagent Kit
- Cleaners
- 1.9 MATERIAL SAFETY DATA SHEETS (MSDS)
- INSTALLATION
- 2.1 GENERAL
- CAUTION Possible system damage can occur if you uncrate the instrument, install it or set it up. ...
- Your instrument is tested before it is shipped from the factory. International symbols and specia...
- Carefully inspect all cartons when they arrive. If you see any sign of mishandling or damage, fil...
- 2.2 SPECIAL REQUIREMENTS: HARDWARE
- Install and operate this instrument in a conventional clinical laboratory environment. Since the ...
- Space and Accessibility
- Electrical Input
- CAUTION Introduction of electrical interference causing the instrument to lock up or reset freque...
- This instrument requires:
- r An independent protected circuit
- r A three-wire outlet furnishing the applicable line voltage, single-phase input power
- r A ground path capable of carrying the full current of the circuit (confirmed thirdwire earth gr...
- r That the 3-m (10-ft) primary power cord on the rear of the Power Supply be plugged directly int...
- Current-carrying capacity of 20 A is recommended, although the actual power consumption is about ...
- Building outlets must be properly grounded and transients protected.
- Ambient Temperature and Humidity
- Air Conditioning
- Ventilation
- Drainage
- CAUTION Incomplete waste chamber drainage and eventual waste chamber overflow into the vacuum sys...
- WARNING Biohazardous contamination could occur from contact with the waste container and its asso...
- The maximum waste line length is 3.7 m (12 ft). The waste drain tubing (rear panel of the Diluter...
- When using an open drain instead of a waste container:
- Be sure to dispose of waste in accordance with environmental protection regulations.
- 2.3 INTERUNIT CONNECTIONS
- Power and Signal Cables
- Changing 9-pin and 25-pin connectors
- Pneumatic/Hydraulic Tubing Connections
- CAUTION Possible reagent siphoning effect and priming problems can occur if a reagent container i...
- IMPORTANT Placing Reagent Paks in any location other than on the counter next to the instrument m...
- Figure2.5 shows the updated tubing connections between the Diluter and the:
- Figure 2.5 Pneumatic/Hydraulic Connections
- Table 2.1 Description of Pneumatic/Hydraulic Connections
- 2.1 GENERAL
- OPERATION PRINCIPLES
- 3.1 COULTER METHOD
- CBC Analysis
- The Coulter method counts and sizes cells by detecting and measuring changes in electrical resist...
- Each cell suspended in a conductive liquid (diluent) acts as an insulator. As each cell goes thro...
- While the number of pulses indicates particle count, the size of the electrical pulse is proporti...
- Differential Analysis
- Effect of Reagents
- Reticulocyte Analysis
- CBC Analysis
- 3.2 AUTOMATIC ASPIRATION MODE
- The system automatically transports, mixes, aspirates and processes specimens.
- Loading Specimens
- Transporting Cassettes
- The system transports each cassette from the loading bay to the sampling station.
- 1. The right lift platform (Figure 3.2) rises beneath the stacked cassettes in the loading bay.
- 2. The bottom cassette is deposited on the platform.
- 3. The platform lowers the cassette to the level of the rocker bed.
- 4. The cassette moves onto the rocker bed where it rocks back and forth, mixing the specimens.
- 5. The cassette moves toward the sensing station until it reaches the tube sensor.
- 6. When the first tube is sensed, the stripper plate locks onto the tube.
- 7. After at least 14 rocks from the time the cassette was loaded, the rocker bed locks in a 45-de...
- 8. At the sampling station, the tube is locked in position.
- 9. The tube ram pushes the tube out from the cassette, causing the needle to pierce the tube stop...
- 10. The bar-code reader scans the cassette and tube labels on both its forward and return passes;...
- r If the two bar-code scans are identical, the bed continues to rock, and the cassette moves forw...
- r If the bar-code reader cannot read the cassette bar-code label, the Diluter stops, posts a mess...
- r If Cass/Pos is the Positive Identifier, and the bar-code reader cannot read the tube bar-code l...
- 11. After the last tube in the cassette is aspirated, the cassette continues along the rocker bed...
- Aspiration
- At the sampling station, after the cap is pierced:
- 1. A pump draws a maximum of 300 µL (LH 700 Series only) or 550 µL (LH 700 Series SlideMaker) of ...
- 2. The blood detectors monitor the passage of sample through the BSV and aspiration lines.
- 3. The needle is withdrawn and the sample tube is reseated in the cassette.
- Delivery
- CBC
- After the sample is aspirated:
- r The center section of the BSV rotates and segments the sample into two separate volumes.
- r Beginning a few seconds before the delivery of the dilutions to the appropriate baths, 5 psi of...
- r The pressure continues during delivery and forms bubbles that mix each cell suspension before s...
- r At the beginning of the delivery, any residual rinse in the Hgb cuvette drains into the waste c...
- r Diluent from the diluent dispensers drives the separated volumes of sample from the BSV to the ...
- r One volume of sample, 1.6 µL, is delivered with 10 mL of diluent to the RBC bath. This dilution...
- r The other volume, 28 µL, is delivered with 6 mL of diluent to the WBC bath. This dilution is us...
- r During delivery to the WBC bath, 1 mL of lytic reagent is added to the dilution to lyse the red...
- r At the same time the lytic reagent is dispensed, 5 mL of diluent from the backwash tank is tran...
- r The final dilution in the WBC bath is 1 part whole blood in a total volume of 251 parts. The fi...
- r The vent section of the piercing needle is rinsed, then dried by high vacuum.
- r The center section of the BSV returns to the aspirate position.
- After the sample is aspirated:
- CBC
- Differential (Diff)
- During aspiration of the blood sample, blood is pulled through two shear valves before it reaches...
- After aspiration of the blood sample, the sliding plate of the diff segment valve (shear valve) m...
- The diff lytic reagent pump dispenses diff lytic reagent, displacing the reagent in the diff heat...
- In the mixing chamber, the blood and diff lytic reagent mixture is incubated and mixed.
- Then the diff preservative pump dispenses diff preservative into the mixing chamber. The final di...
- Diff sample pressure is applied to the mixing chamber to push the final incubated dilution of sam...
- Before the next sample is analyzed, the diff sample segment, the mixing chamber, and the flow cel...
- The diff lytic reagent pathways to and from the diff sample valve remain primed with diff lytic r...
- Reticulocyte (Retic)
- During aspiration of the blood sample, blood is pulled through two shear valves before it reaches...
- After aspiration of the blood sample, the sliding plate of the retic segment value (shear valve) ...
- The stain pump dispenses retic stain through the retic segment valve, washing the retic segment i...
- After incubation, the 50 µL blood /stain aspiration pump is activated twice to pull a stained sam...
- The retic clearing solution pump dispenses 2 mL of retic clearing solution, displacing the reagen...
- In the retic chamber, the stained blood and retic clearing solution mixture is mixed and incubated.
- Retic sample pressure is applied to the retic chamber to push the mixture from the retic chamber ...
- Before the next sample is analyzed the retic segment tubing, retic chamber and flow cell are rins...
- In addition, the instrument activates the blood/stain pump to remove residual stained blood from ...
- CBC Sensing System
- Vacuum, equal to 6 in. of mercury, draws a precise volume of suspension from each bath through th...
- When the vacuum starts to draw the suspension, current is supplied to the electrode. The electric...
- While the sample in each bath is sensed, the photometer reads the Hgb-blank and the Analyzer reta...
- CBC Analysis in the Baths
- The RBC and Plt data is generated by the RBC bath. The WBC and Hgb data is generated by the WBC b...
- In the analyzer the Red/White Processor counts and sizes the RBC and WBC data. The PLT Processor ...
- The workstation then:
- r Corrects the RBC and WBC raw counts for wait time and coincidence.
- r Fit the PLT histogram and corrects the PLT histogram count for coincidence.
- r Calculates HGB from the blank and sample readings.
- r Scales for calibration and dilution.
- r Performs voting on the three apertures for RBC, WBC, PLT.
- r Derives parameters from the RBC histogram; MCV and RDW.
- r Derives the MPV and @PDW parameters from the Plt histogram.
- r Derives the calculated parameters.
- r Displays results and histograms.
- Diff and Retic Multiparameter Sensing System
- The flow cell is located within the Triple Transducer Module (TTM). The TTM produces three measur...
- Figure 3.3 shows the Triple Transducer Module and its protective housing as it resides in the Dil...
- Tamper-proof screws secure the protective housing; they can only be removed with a special tool.
- The laser is a helium-neon laser that complies with the United States' performance standard for l...
- Figure 3.4 Triple Transducer Module with Protective Housing Cut Away
- Backwash and Rinse
- 3.3 MANUAL ASPIRATION MODE
- IMPORTANT Clots in the specimen can cause misleading results. The blood detectors are not active ...
- The Manual mode of operation is like the Automatic mode except:
- r Before you run the sample, you enter the sample identification number by performing any of the ...
- r You use an open vial and introduce the sample at the aspirator tip.
- r You begin the cycle by obstructing the optical sensor’s beam
- r The system aspirates a maximum of 200 µL of sample.
- r The blood detectors are not active in the Manual mode.
- 3.4 COUNTING AND SIZING
- Red and White Blood Cell Counting
- The RBC and WBC baths each have three discrete apertures that function as independent systems. Th...
- Regulated vacuum draws a precise volume of sample dilution through the apertures. At each apertur...
- The system sends these pulses to the RED/WHITE PROCESSOR card. The RED/WHITE PROCESSOR card count...
- The workstation then:
- r Corrects the RBC and WBC raw counts for wait time and coincidence.
- r Fit the PLT histogram and corrects the PLT histogram count for coincidence.
- r Calculates HGB from the blank and sample readings.
- r Scales for calibration and dilution.
- r Performs voting on the three apertures for RBC, WBC, PLT.
- r Derives the parameters from the RBC histogram; MCV and RDW.
- r Derives the MPV and @PDW parameters from the Plt histogram and @Pct and @PDW.
- r Derives the calculated parameters.
- r Displays results and histograms.
- Coincidence Correction
- Voting
- To prevent data errors due to statistical outliers or obstructions that may block an aperture, th...
- If the data from one aperture is outside the established statistical range, the computer votes ou...
- The data from at least two of the three apertures must be within an established statistical range...
- Pulse Editing
- Sweep Flow
- RBC Size Distribution
- After editing, the RED/WHITE PROCESSOR converts the three sets of both the RBC and WBC pulses. It...
- To ensure that the size-distribution curve accurately reflects the true cell population, the syst...
- After the sensing periods are completed, the system sends these histograms through the Communicat...
- Platelet Method
- Pulses representing cells from 2 to 20 fL are classified as platelets. The analyzer sorts the pul...
- The analyzer sends this information is sent to the Workstation. At the Workstation, all histogram...
- 1. The PLT is > 20,000 in the raw histogram.
- 2. The curve is positive and unimodal.
- 3. The mode is between 3 and 15 fL.
- 4. The @PDW is < 20
- 5. The height of pulses in channels 0 and 1 is < 1.5 times the height of the mode channel.
- The PLT is then calculated as the area under the fitted histogram.
- Special conditions include, for example, the presence of microcytic red cells or red cell fragmen...
- For samples that are not special cases, but that fail normal curve fitting criteria, the raw hist...
- The initial count is then coincidence corrected, voted on, averaged.
- The histograms are also voted on and averaged.
- Incomplete and overrange conditions are noted.
- The counts are adjusted for calibration and predilute factors.
- MPV and @PDW are derived, and @Pct is calculated.
- For flagging, all platelet data, plus data from the WBC and RBC histograms is further examined. T...
- It is possible to observe:
- Red and White Blood Cell Counting
- 3.5 MEASUREMENT OF HEMOGLOBIN CONCENTRATION
- 3.6 DATAPLOT DEVELOPMENT
- At the Workstation, raw data is analyzed to:
- The Workstation performs a series of operations on the stored digital raw values received from th...
- In the LH 750, the Beckman Coulter AccuGate algorithm uses adaptive contouring methods designed f...
- In the DataPlots, different colors represent different memberships (types of cells). Shades of co...
- Two-Dimensional (2D) DataPlots
- Three-Dimensional (3D) DataPlots
- 3.7 PARAMETERS AND THEIR DERIVATION
- Mathematical expressions in this section are in U.S. units of measurement. Parameter units can be...
- Summary of Parameter Derivations
- White Blood Cell (WBC) Count
- Red Blood Cell (RBC)
- Hemoglobin (Hgb) Concentration
- Mean Corpuscular Volume (MCV)
- Hematocrit (Hct)
- Mean Corpuscular Hemoglobin (MCH)
- Mean Corpuscular Hemoglobin Concentration (MCHC)
- Red Cell Distribution Width (RDW)
- Platelet (Plt) Count
- Mean Platelet Volume (MPV)
- NRBC % (Nucleated Red Blood Cells)
- NRBC # (Nucleated Red Blood Cells)
- Differential (Diff)
- Reticulocyte (Retic) Parameters
- 3.8 XB ANALYSIS
- Studies (Bull 1974, Koepke 1981) indicate that the red cell indices (MCV, MCH, and MCHC) of patie...
- Here is the XB formula.
- Where:
- X(B,i)
- = ith XB value
- X(B, i-1)
- = (i-1)th XB value
- X(j,i)
- = the jth X value in the ith batch
- SGN
- = the arithmetic sign of number in parentheses
- N
- = number of samples in the batch
- x
- = symbol used to represent multiplication
- The formula is easily implemented with a computer. Its function is to enable reliable estimates o...
- The hematology system is considered "in control" when the batch means are within established limi...
- The LH Workstation calculates and displays the percent difference between each batch mean and its...
- MCV
- MCH
- MCHC
- Adjusting Initial XB Target Values
- The recommended target values for initial entry are:
- As samples are run and laboratory values established, the recommended target values can be adjust...
- If the CVs are greater than 1.5%, or the mean values are greater than 3% different from the recom...
- If the XB indices are still out-of-limits, you should investigate the instrument and reagent syst...
- Table 3.1 Effect of Directly-Measured Parameters on the Red Cell Indices
- XM Analysis
- Extended QC
- 3.1 COULTER METHOD
- SPECIFICATIONS/CHARACTERISTICS
- 4.1 PHYSICAL SPECIFICATIONS
- 4.2 PERFORMANCE SPECIFICATIONS--LH 700 Series
- The LH 700 Series consists of three subsystems, designated as "CBC" (Complete Blood Count), "WBC ...
- Performance specifications stated apply only to an instrument that has been properly maintained a...
- If the average room temperature should change more than 5.5˚C (10˚F) from the calibrating tempera...
- Precision
- Accuracy
- Linearity
- Background
- Carryover
- Operating and Reportable Ranges
- Mode-to-Mode Comparison
- 4.3 PERFORMANCE CHARACTERISTICS
- The information in this section describes typical performance for an instrument that has been pro...
- Sample Stability
- The following tables show the average results for specimens from five normal donors collected in ...
- Table 4.7 CBC Sample Stability, Room Temperature‡
- Table 4.8 CBC Sample Stability, Cold Temperature‡
- Table 4.9 DIFF% Sample Stability, Room Temperature‡
- Table 4.10 DIFF% Sample Stability, Cold Temperature‡
- Table 4.11 DIFF# Sample Stability, Room Temperature‡
- Table 4.12 DIFF# Sample Stability, Cold Temperature‡
- Table 4.13 RETIC Sample Stability, Room Temperature‡
- Table 4.14 RETIC Sample Stability, Cold Temperature‡
- Table 4.15 NRBC Sample Stability, Room Temperature‡
- Table 4.16 NRBC Sample Stability, Cold Temperature‡
- WBC Differential Flagging Stability
- NRBC Paired Sample Imprecision
- MCV Accuracy Characteristics
- NRBC Accuracy Characteristics
- NRBC Accuracy Characteristics was defined as the agreement between the LH 700 Series and the resu...
- All NRBC % results > 2 and < 15 will be flagged with an R symbol. Beckman Coulter, Inc. recommend...
- Table 4.21 shows the NRBC results for whole blood specimens with and without NRBC collected in a ...
- Table 4.21 NRBC Accuracy Characteristics, Compared Samples
- Platelet Accuracy Characteristics
- Reference Ranges
- Storing Specimens
- Sampling Modes
- Throughput, Automatic Mode
- Sample Volume Aspirated
- Waste
- Pneumatic Supplies (Internally Regulated)
- Calibration Stability
- LH Workstation Storage
- Known Interfering Substances
- 4.4 BAR-CODE SYMBOLOGY OVERVIEW
- A bar-code symbol is a series of adjacent bars and spaces. The widths of the bars and spaces repr...
- A symbology is defined by the following characteristics:
- Character Set
- Symbology Type
- Fixed or Variable Length
- Self-Checking
- Start Code, Stop Code
- Check Character, Checksum Algorithm
- Quiet Zone, Quiet Area
- 4.5 BAR CODES AND THE LH 700 Series
- IMPORTANT
- 1. DO NOT use the following characters # @ [ \ ] ` { | } ~ in Specimen or Patient identifiers. Th...
- 2. Risk of misidentification. Use of poor quality, dirty, improperly placed or damaged bar-code l...
- 3. DO NOT use leading or trailing spaces in the ID.
- The LH 700 Series supports the use of up to 16-character bar-code labels and a wide variety of ba...
- The following information is intended as a guide to help you in your selection process.
- Checksum Algorithm
- Bar-Code Symbologies Supported by the LH 700 Series
- Interleaved 2-of-5
- Code 39 (Also called 3-of-9 Code)
- Codabar
- NW-7
- Code 128/USS 128
- Code 128 is a continuous, variable length symbology. This symbology has 106 different printed cha...
- Code 128 is character dependent. See AIM Uniform Symbol Specification (USS) Rev. 1986 for additio...
- You must use and print a checksum character, and it must conform to the AIM USS 128 checksum gene...
- Bar-Code Tips
- 4.6 BAR-CODE LABEL SPECIFICATIONS
- IMPORTANT
- 1. DO NOT use the following characters # @ [ \ ] ` { | } ~ in Specimen or Patient identifiers. Th...
- 2. Risk of misidentification. Use of poor quality, dirty, improperly placed or damaged bar-code l...
- 3. DO NOT use leading or trailing spaces in the ID.
- General
- Optical Characteristics at 880 nm ±10% and 633 nm ±10%
- Printing Method
- Label Thickness
- NE/WE Ratio
- Must remain constant over code length.
- Label Dimensions and Data
- Figure 4.1 Bar-Code Label Specifications
- Table 4.24 Bar-Code Label Specifications
- Acceptable Bar Codes
- Extended Digit Bar Code
- When using bar-coded data lengths from 12 to 16 data characters, consider these recommendations:
- r Use a tube that can accommodate a label length between 53.34 mm (2.1 in.) and 63.50mm (2.5 in....
- r Use checksums to provide protection against occasional misread errors caused by problems such a...
- r Maintain the bar-code printer. To achieve the maximum number of digits on the bar-code labels, ...
- r Print bar code patterns on labels to allow for a minimum of 10 times the narrow element width a...
- r Place the bar-code label on the tube to ensure that the printed area, including the quiet zones...
- r Configure your hand-held scanner for the specific bar code type as instructed in your Online Help.
- When using bar-coded data lengths from 12 to 16 data characters, consider these recommendations:
- Table 4.25 Code-Related Specifications
- Checksum Algorithm
- Beckman Coulter strongly recommends the use of bar-code checksums to provide automatic checks for...
- The algorithm for determining the checksum for each code is as follows:
- Interleaved 2-of-5
- This code requires 3 to 15 data digits plus a checksum or 16 digits without checksum. The number ...
- To determine the value of the checksum character:
- 1. Identify even- and odd-positioned characters in the message with the right-hand message charac...
- 2. Sum the numeric values of the odd-positioned characters.
- 3. Sum the numeric values of the even-positioned characters and multiply the total by 3.
- 4. Sum the odd and even totals from steps 2 and 3.
- 5. Determine the smallest number which, when added to the sum in step 4, results in a multiple of...
- 6. This number is the value of the checksum character.
- 7. Determine whether total number of characters (message plus checksum) is odd or even. If odd, a...
- Codabar
- Table 4.26 Codabar Character Value Table
- Table 4.27 NW 7 Character Value Table
- The checksum technique is:
- WEIGHTED MODULUS 11:
- Checksum Technique:
- Example 1: Message 011529007
- Use weight (1):
- (9 x 0) + (10 x 1) + (8 x 5) + (4 x 2) + (5 x 9) + (3 x 0) + (6 x 0) + (2 x 7) = 124
- 124 ÷ 15 = 11, remainder 3
- When the remainder is 0, 0 is the check digit.
- 11 - 3 = 8
- The value 8 corresponds to the character 8, Therefore the Checksum is 8 and the final decoded mes...
- Example 2: Message 023229006
- Use weight (1):
- (9 x 0) + (10 x 2) = (7 x 3) + (8 x 2) + (4 x 2) + (5 x 9) + (3 x 0) + (6 x 0) + (2 x 6) = 122
- 122 ÷ 11 = 11, remainder 1
- When the remainder is 1, the calculation must be repeated using weight (2).
- Use weight (2):
- (2 x 0) + (10 x 2) + (4 x 3) + (3 x 2) + (7 x 2) + (6 x 9) + (8 x 0) + (5 x0) + (9 x 6) = 160
- 160 ÷ 11 = 14, remainder 6
- When the remainder is 0, 0 is the check digit.
- 11 - 6 = 5
- The value 5 corresponds to the character 5, Therefore the Checksum is 5 and the final decoded mes...
- :
- Table 4.28 Code 39 Character Value Table:
- The checksum technique is:
- Example:
- Code 128
- This code uses four to 16 data digits on the LH 700 Series and four to 11 data digits for the Sli...
- The checksum character immediately precedes the stop character. The checksum character used with ...
- For example, a label contains a START character (Code C), Data (25), a Check character and a STOP...
- The checksum is equal to 130 modula 103 (the remainder of 130 divided by 103):
- Therefore the check character equals character value 27, which is; in Code Set A.
- For additional information on this procedure, refer to AIM USS-128 Rev. 1986, published by AIM, I...
- HAZARDS
- 5.1 LASER SAFETY
- WARNING Possible harm to operator. Do not use any controls, make any adjustments, or perform any ...
- The Triple Transducer Module and Bar-Code Reader contain lasers. A laser is a unique light source...
- All service and maintenance of the laser must be done at the Beckman Coulter factory by trained p...
- 5.2 RADIATION HAZARDS
- 5.3 LASER WARNING LABELS
- WARNING Possible harm to operator. This instrument contains components dangerous to the operator....
- CDRH-approved labels are placed near or on those covers that, when removed, might expose laser ra...
- Note: As installed in the Triple Transducer Module (TTM) safety fixture, the laser presents no ra...
- 5.4 BAR-CODE READER
- 5.5 HANDHELD SCANNER
- 5.6 DISPOSAL OF ELECTRICAL INSTRUMENTATION
- It is very important that customers understand and follow all laws regarding the safe and proper ...
- The symbol of a crossed-out wheeled bin on the product is required in accordance with the Waste E...
- 1. 1. that the device was put on the European Market after August 13, 2005 and
- 2. 2. that the device is not to be disposed via the municipal waste collection system of any memb...
- 5.1 LASER SAFETY
- REFERENCES
- 1. Coulter WH. High speed automatic blood cell counter and cell size analyzer. Paper presented at...
- 2. Brecher G, Schneiderman M and Williams GZ. Evaluation of electronic red blood cell counter. Am...
- 3. Brittin GM, Brecher G and Johnson CA. Evaluation of the COULTER COUNTER Model S. Am J Clin Pat...
- 4. Gottman AW. Multiple hematologic analyses by means of a COULTER COUNTER Model S. Paper present...
- 5. Hamilton PJ and Davidson RL. The interrelationships and stability of Coulter S-determined bloo...
- 6. Bessman JD and Johnson RK. Erythrocyte volume distribution in normal and abnormal subjects. Bl...
- 7. Price-Jones C. The diameters of red cells in pernicious anaemia and in anaemia following haemo...
- 8. England JM, Walford DM and Waters DAW. Re-assessment of the reliability of the haematocrit. Br...
- 9. Bull BS, Schneiderman MA and Brecher G. Platelet counts with the COULTER COUNTER. Am J Clin Pa...
- 10. Mundschenk DD, Connelly DP, White JG and Brunning RD. An improved technique for the electroni...
- 11. Schultz J and Thom R. Electrical sizing and counting of platelets in whole blood. Med Biol En...
- 12. Von Behrens WE. Mediterranean macrothrombocytopenia. Blood, 1975; 46(2):199-208.
- 13. Paulus JM. Platelet size in man. Blood, 1975; 46(3):321-336.
- 14. International Committee for Standardization in Haematology. Recommendations for reference met...
- 15. Gauthier J, Harel P, Belanger C and Fraysse J. Human leukocytes: their size distribution and ...
- 16. Hughes-Jones NC, England JM, Norley I and Young JMS. Differential leucocyte counts by volume ...
- 17. England JM, Bashford CC, Hewer MG, Hughes-Jines NC and Down MC. A semi-automatic instrument f...
- 18. Wycherley PA and O'Shea MJ. Abridged differential leucocyte counts provided by a Coulter Chan...
- 19. Oberjat TE, Zucker RM and Cassen B. Rapid and reliable differential counts on dilute leukocyt...
- 20. Hoffman RA and Britt WB. Flow-system measurement of cell impedance properties. J Histochem Cy...
- 21. Leif RC, Scwartz S, Rodriguez CM, Peel-Fernandez L, Groves M, Leif SB, Cayer M and Crews H. T...
- 22. Coulter WH et al. Signal modulated apparatus for generating and detecting resistive and react...
- 23. Fulwyler MJ. Electronic separation of biological cells by volume. Science, 1965; 150:910-911.
- 24. Loken MR, Sweet RG and Herzenberg LA. Cell discrimination by multiangle light scattering. J H...
- 25. Jovin TM, Morris SJ, Striker G, Schultens HA, Digweed M and Arndt-Jovin DJ. Automatic sizing ...
- 26. Miale JB. Laboratory Medicine: Hematology. CV Mosby Company, St. Louis, MO, 4th ed., 1972; 22.
- 27. Corash L, Rheinschmidt M, Lieu S, Meers P and Brew E. Fluorescence-activated flow cytometry i...
- 28. Friedman EW. Reticulocyte counts: How to use them, what they mean. Diagnostic Medicine, 1984;...
- 29. Williams WJ, Beutler E, Erslev AJ, and Lichtman MA. Hematology. McGraw-Hill, Inc, New York, N...
- 30. Brecher G. New methylene blue as a reticulocyte stain. Am J Clin Path, 1949; 19:895-896.
- 31. Dorsey DB. What can quality control do for hematology? Am J Med Tech, 1965; Mar-Apr:150-153.
- 32. Bull BS. A statistical approach to quality control. Quality Control in Hematology, Symposium ...
- 33. Koepke JA and Protextor TJ. Quality assurance for multichannel hematology instruments. Am J C...
- 34. Bull BS. A statistical approach to quality control. Quality Control in Hematology, Symposium ...
- 35. Eckhoff RF. An experimental indication of the volume proportional response of the Coulter Cou...
- 36. Grover NB, Naaman J, Ben-asson S and Dojanski F. Electrical sizing of particles in suspension...
- 37. Waterman CS, Atkinson EE, Wilkins B, Fischer CL and Kimsey SL. Improved measurement of erythr...
- 38. Kachel V and Ruhenstroth-Bauer G. Methodik and Ergebissne Optiseher Formfatorunter-suchungen ...
- 39. Bull BS, and Elashoff RM et al.: 1974. A study of various estimators for the derivation of qu...
- 40. Koepke JA. Tips on technology. MLO:15, 1981.
- 41. NCCLS document H20-A. Approved Standard for the Evaluation of the Differential Cell Count. Na...
- 42. Miale JB, Laboratory Medicine - Hematology. 3rd Edition 1967, CV Mosby, pages 592-595
- 43. NCCLS document H44-A. Approved Guideline for Methods for Reticulocyte Counting (Flow Cytometr...
- 44. Moser K., Seelenbinder F., McFadden S., Adkins C., Goshay M., Davis F., Selecting a New Analy...
- 45. Kaplan S., Johnson K., Wolfe N., Validation of Low Platelet Counts from Coulter LH 750 and Co...
- 46. Steele B., Wu N., Whitcomb C., White Blood Cell and Platelet Counting Performance by Hematolo...
- 47. Charie L., Harrison P., Smith C., Cobb J., Briggs C., Machin S., Accuracy in the Low Platelet...
- 48. Yee I., Keeney M., Johnson K., Brown W., Wolfe N., Kaplan S., White Blood Cell Flagging Rates...
- 49. Keeney M., Brown W., Yee I., Platelet Counts and Flagging Rates from the LH 750 Hematology An...
- 50. Fernandez T., Domack L., Montes D., Pineiro R., Landrum E., Vital E., Performance Evaluation ...
- 51. Brown W., Kaplan S., Keeney M., Johnson K., Wolfe N., Yee I., Workflow Improvement with Rando...
- 52. Richtlinie der Bundesärztekammer zur Qualitätssicherung quantitativer laboratoriumsmedizinisc...
- 53. Bowles K. M., Cooke L.J., Richards E.M., Baglin T.P, Platelet size has diagnostic predictive ...
- 54. Bancroft A. J., Abel E. W. , Mclaren M. , Belch J. J. F., Mean platelet volume is a useful pa...
- *National Committee for Clinical Laboratory Standards has changed to Clinical and Laboratory Stan...
- GLOSSARY
- µL
- Microliter, a unit of volumetric measurement equal to one millionth of a liter.
- µm
- Micrometer, a unit of linear measurement equal to one millionth of a meter.
- A
- Ampere, a unit of electric current.
- Accuracy
- Ability of the instrument to agree with a predetermined reference value.
- Action Limits
- Values established by your laboratory to flag results requiring action.
- Active Set
- For Flagging Limits, Decision Rules and Auto Validation logic, the Active Set of parameters are d...
- r are enabled for the System (System Setup – Patient – Parameters window),
- r are enabled for the Report Profile you are using (System Setup – Patient –Reporting Options win...
- r are part of the Test Mode you are running (C, CD, CDR, CR, or R on the Command Center) or have ...
- r are included in the parameter block of the validation code you are looking for.
- ADMS Workstation
- The computer hardware and software that controls instrument operation; displays, stores, and reca...
- Also called: ADMS, DMS, LH Workstation.
- Algorithm
- A particular procedure for performing an analysis.
- Analytical Station
- The Analyzer, Diluter, and Power Supply of the LH 700 Series.
- Analyzer
- The Analyzer is a subsystem of the LH 700 Series that physically sits on top of the Diluter.
- Analyzer Pulse
- A graphical display on the Analyzer screen that indicates the condition of all the apertures. You...
- ANSI
- American National Standards Institute.
- Application
- A computer program, generally started from a button or icon. For example, starts the Patient Test...
- ASCII
- American Standard Code for Information Interchange.
- Aspiration Mode
- The method of running a sample, closed vial (Automatic aspiration mode) or open vial (Manual aspi...
- Assay Values
- Values of all parameters for a control established by extensive assay of that control. These valu...
- Also called: assigned values.
- Assigned Value
- Values of all parameters for a control established by extensive assay of that control. These valu...
- Also called: assay values.
- ASTM
- American Society for Testing and Materials.
- AutoCollation
- A Workstation feature that automatically combines results of different test modes (CBC/Diff and R...
- Automatic Aspiration Mode
- The closed-vial method of running a sample. You place a closed tube sample in a cassette and plac...
- Automatic Startup Cycles
- When you press the Startup button, the system flushes the cleaning agent from all Diluter compone...
- AutoNumbering
- A Workstation feature that assigns a number to each sample received from the instrument.
- Autopurge
- If 24 hours elapse with the power on, pneumatics off, and the instrument in shutdown, the system ...
- AutoSequencing
- A Workstation feature used with the ToDo list to automatically increment the identifiers you spec...
- AutoStop
- A Workstation feature that instructs the instrument to stop processing based on specific criteria.
- Average Value
- A value that is computed by dividing the sum of a set of values by the number of values. Usually ...
- BA # (Basophils)
- A WBC differential parameter result from the Diff sample analysis. The number (#) is computed fro...
- BA % (Basophils)
- A WBC differential parameter result from the Diff sample analysis. The percent (%) is measured di...
- Background Count
- A measure of the amount of electrical or particle interference using a diluent sample or no sample.
- Background Results
- Background results indicate reagent quality and the presence of electronic noise or bubbles. Back...
- Batch
- A group or set of results.
- Batch Mean
- The mean or average of a set of samples. For Xb and XM Analysis, the batch mean is a value based ...
- Batch Print Job
- Selecting multiple items from the database, then selecting the print icon to print all of them at...
- Baud
- A rate defining how many data bits per second are transferred during communications between two p...
- Calibration
- A procedure to standardize the instrument for accuracy by determining its deviation from calibrat...
- Calibration Disk
- A data diskette containing the Assigned Values for the lot number of the S-CAL calibrator kit bei...
- Calibration Factors
- Values the system uses to fine-tune instrument accuracy.
- Carryover
- The percentage (WBC, RBC, Hgb and Plt) or particle count (for Diff and Retic) of blood cells that...
- Cass/Pos
- The Workstation considers this field an optional identifier for the cassette number and cassette ...
- Cassette
- The cassette is the carrier for the sample tubes (patient, control, or special test) used in Auto...
- Cassette Clips
- Clips that you permanently install in each opening of the special cassettes for tubes with HEMOGA...
- CBC (Complete Blood Count)
- A measure of the cell number and the indices. CBC includes WBC, RBC, Hgb, Hct, MCV, MCH, MCHC, Pl...
- CDC
- Centers for Disease Control and Prevention.
- CEE
- Commission for Electrical Equipment.
- Cell Control
- A preparation made of human blood with stabilized cells and surrogate material. It is used for da...
- Characters
- All letters A-Z and numbers 0-9.
- Check Valve
- A one-way valve that routes liquid or air through the Diluter.
- Also called: mono-flow valve, one-way valve
- CLIA
- Clinical Laboratory Improvement Amendments.
- Closed-vial Mode
- The closed-vial method of running a sample. You place a closed tube sample in a cassette and plac...
- Also called: closed-vial mode, primary mode, automatic sampling mode.
- CLSI
- Clinical and Laboratory Standard Institute formerly known as the National Committee for Clinical ...
- cm
- Centimeter, a unit of linear measurement.
- Codes
- On windows and printouts, symbols, such as -----, ++++, :::::, appear with or in place of sample ...
- Coefficient of Variation
- An expression, in percent, of data spread as related to the mean.
- Coincidence
- More than one cell within aperture-sensing boundaries at the same time. The system counts only on...
- Collation
- The process of combining the results of different test modes(CBC/Diff and Retic) analyzed from th...
- Command Center
- A teal bar that appears at the bottom of the screen.
- Control Disk
- A data diskette that contains the assay values for the lot number of the control being used.
- Coulter Principle
- A method of counting and sizing cells by detecting and measuring changes in electrical resistance...
- Critical Limits
- Values established by your laboratory to flag results requiring immediate action.
- CSA
- Canadian Standards Association.
- CSF
- Cerebrospinal fluid is a clear, colorless, non-cellular fluid that circulates over the brain and ...
- Cursor
- On the screen, a place shown by a little blinking indicator or by a highlighted area. The cursor ...
- CV (Coefficient of Variation)
- An expression, in percent, of data spread as related to the mean.
- Cycle Counter
- A number that appears on the Analyzer MAIN MENU screen that represents the actual number of instr...
- DataPlot
- A graphic representation of results. DataPlots present a combined view of population density and ...
- Data Management System (DMS)
- The computer hardware and software that controls instrument operation; displays, stores, and reca...
- Also called: ADMS, DMS, LH Workstation.
- Default
- A setting the instrument uses automatically. For example, you can set up a default printer. Every...
- Definitive Messages
- Definitive messages appear in a separate area of the screen display, printout, and host transmiss...
- Delta Check
- A check on sample results that is made by clinical laboratories to determine if the current resul...
- Density
- The number of cells in a particular region, regardless of the type of cell.
- On DataPlots, as more cells appear in a region, the color of the region gets brighter.
- Diff (Differential)
- Leukocyte differential parameters (NE, LY, MO, EO, and BA and processes that relate to them.
- Digits
- All numbers 0-9.
- Diluter
- This is the fluidics portion of the LH 700 Series System. The Diluter is the subsystem that aspir...
- dL
- Deciliter, a unit of volumetric measurement equal to 0.1 liter.
- DMS
- The computer hardware and software that controls instrument operation; displays, stores, and reca...
- Also called: ADMS, DMS, LH 700 Series Workstation.
- Double-click
- If you are using:
- Drag
- The process of selecting an item and maintaining the selection while you move the pointer to a di...
- EDTA
- Ethylenediaminetetraacetic acid--the recommended anticoagulant for hematology analysis.
- EO # (Eosinophils)
- A WBC differential parameter result from the Diff sample analysis. The number (#) is computed fro...
- EO % (Eosinophils)
- A WBC differential parameter result from the Diff sample analysis. The percent (%) is measured di...
- Expiration Date
- A manufacturer’s recommended last day of use for a reagent, control, or calibrator.
- Extended QC
- Extended QC Rules for 5C Cell control derived from the German Quality Control Guidelines for the ...
- Extended QC - Random Error
- Random error is Extended QC’s measurement of imprecision.
- Extended QC - Systematic Error
- Systematic error is Extended QC’s measurement of bias. A systematic error is defined as the devia...
- Extended QC - Total Error
- Total Error is Extended QC’s measurement of inaccuracy, as compared to an established limit.
- Total Error is defined as the deviation of a single measurement from the Target Value that was se...
- Field
- Area on a window or screen for entering or viewing data. When you move the cursor, you are moving...
- First Name
- Referes to the patient’s first name. The Workstation considers this field an optional identifier ...
- fL
- Femtoliter, a unit of volumetric measurement equal to liter.
- Flags
- A flag is a single letter or symbol and will always appear to the right of a result. A flag can b...
- Flow Cytometry
- A process for measuring the characteristics of cells or other biological particles as they pass t...
- Function Key
- One of the keys labeled F1 to F12. To request a system or window-specific command, press the Func...
- g
- Gram, a unit of weight.
- HCT (Hematocrit)
- A computed value that represents the packed cell volume (PCV) relative to a given volume of whole...
- Hemoglobinometry
- Measurement of hemoglobin in the blood. In the LH 700 Series, this is done by comparing the amoun...
- HGB (Hemoglobin)
- Hemoglobin results from the CBC analysis. This parameter is measured directly using Photometric M...
- Hgb Voltages
- Electronic voltages measured through the hemoglobin cuvette.
- Histograms
- Graphic representations of cell frequency vs. size. Histograms provide information about leukocyt...
- Hz
- Hertz, a unit of frequency.
- i.d.
- Inside diameter.
- IEC
- International Electrtechnical Commission.
- Information System
- Any host or laboratory computer system.
- Instrument
- The Analyzer and Diluter portion of the LH 700 Series.
- IQAP (Interlaboratory Quality Assurance Program)
- Beckman Coulter Inc. provides this program, which statistically compares your 5C Cell control and...
- IRF (Immature Reticulocyte Fraction)
- This parameter is derived from VCS technology.
- IRF = High Light Scatter Reticulocytes / Total reticulocytes
- L
- Liter, a unit of volumetric measurement.
- Lab Administrator
- A person at your location who can access the full set of Workstation functions. The lab administr...
- Laser (Light Amplification by Stimulated Emission of Radiation)
- The instrument uses a laser for WBC Diff and Retic analysis.
- Last Name
- Referes to the patient’s last name. The Workstation considers this field an optional identifier y...
- Levey-Jennings Control Chart
- A commonly used control procedure in which control measurements are plotted directly on a control...
- Linearity
- The ability of an instrument to recover expected results (reference values or calculated values) ...
- Beckman Coulter Inc. provides the LIN-C linearity control for your convenience. Use it according ...
- Lot Number
- An identifier assigned by a manufacturer to identify a control, reagent or calibrator.
- LY #(Lymphocytes)
- A WBC differential parameter result from the Diff sample analysis. The number (#) is computed fro...
- LY % (Lymphocytes)
- A WBC differential parameter result from the Diff sample analysis. The percent (%) is measured di...
- m
- Meter, a unit of linear measurement.
- Manual aspiration mode
- The open-vial method of running a sample. Also called: open-vial mode, secondary mode, manual sam...
- Manual Diff Box
- A blank area included on a report where you can record your manual differential results for the s...
- Manual Print Job
- Selecting the print icon to print a single item.
- MCH (Mean Corpuscular Hemoglobin)
- A computed value that represents the amount of hemoglobin by weight in the average red cell. The ...
- MCHC (Mean Corpuscular Hemoglobin Concentration)
- A computed value that represents the concentration of hemoglobin (weight/volume) in the average r...
- MCV (Mean Corpuscular Volume)
- The volume of the average red cell derived from the RBC histogram.
- Mean
- Arithmetic average of a group of data.
- Membership
- The different types of cells in a particular region, regardless of the number of cells.
- On DataPlots, membership is represented showing different types of cells in different colors.
- mL
- Milliliter, a unit of volumetric measurement, equal to liter.
- mm
- Millimeter, a unit of linear measurement, equal to one-thousandth of a meter.
- MO # (Monocytes)
- A WBC differential parameter result from the Diff sample analysis. The number (#) is computed fro...
- MO % (Monocytes)
- A WBC differential parameter result from the Diff sample analysis. The percent (%) is measured di...
- Mode
- The method of running a sample, closed vial (Automatic aspiration mode) or open vial (Manual aspi...
- Modes (Test)
- Use Default Type on the Command Center to specify the test mode. It determines how the LH 700 Ser...
- Mode-to-Mode Matching
- Agreement between patient results in Automatic aspiration mode and Manual aspiration mode.
- MPV (Mean Platelet Volume)
- MPV is the mean cell volume for platelets. The system derives this parameter from the Plt histogram.
- MRV (Mean Reticulocyte Volume)
- Mean volume of the Retic population. This parameter is derived from VCS technology.
- @MSCV (Mean Sphered Cell Volume)
- THIS PARAMETER IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
- This field and parameter label disappear if the parameter was excluded as part of parameter selec...
- Mean volume of red cell population of Reticulocyte analysis. This parameter is derived from VCS t...
- mW
- Milliwatt, a unit of power equal to one-thousandth of a watt.
- n
- Number.
- NCCLS
- National Committee for Clinical Laboratory Standards is now the Clinical and Laboratory Standard ...
- NE # (Neutrophil)
- A WBC differential parameter result from the Diff sample analysis. The number is computed from th...
- NE % (Neutrophil)
- A WBC differential parameter result from the Diff sample analysis. The percent is measured direct...
- NEMA
- National Electrical Manufacturers Association.
- nm
- Nanometer, a unit of linear measurement, equal to meter.
- Nonhematopoietic Cells
- Any of various tissue-based nucleated cells that may be counted by the analyzer as a WBC, but are...
- NRBC # (Nucleated Red Blood Cells)
- A parameter that is calculated from the NRBC % and the total WBC count. NRBC # represents the tot...
- NRBC % (Nucleated Red Blood Cells)
- A parameter derived from both the WBC histogram and VCS information and represents the number of ...
- o.d.
- Outer diameter.
- Operator ID
- A 16-character identifier that uniquely identifies the processor of the samples. If you forget yo...
- Also called: operator ID.
- Open-vial Mode
- The open-vial method of running a sample. You immerse the aspirator tip in the sample. The instru...
- Also called: open-vial mode, secondary mode, manual sampling mode.
- Outlier
- A data value far outside the range of the rest of the data.
- Output Device
- A physical device that is capable of receiving information from a computer and formatting it in a...
- Parameters
- Characteristics of blood that the instrument measures and reports.
- Parity
- Method of detecting errors in data handling. The computer generates a parity bit such that the su...
- Patient ID
- The Workstation considers this field an optional sample identifier.
- Your laboratory may use it as a specific identifier for the patient, such as the medical record o...
- Patient Population
- A large number of patient sample results for Analysis, used to give a fairly consistent average r...
- PCT (Plateletcrit)
- THIS PARAMETER IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
- This field and parameter label disappear if the parameter was excluded as part of parameter selec...
- A computed value that represents the platelet packed cell volume.
- PDW (Platelet Distribution Width)
- THIS PARAMETER IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
- This field and parameter label disappear if the parameter was excluded as part of parameter selec...
- The coefficient of variation of Plt size.
- The system derives this parameter from the Plt histogram. This parameter appears as a percent.
- Pending List
- A feature of the Workstation that lets you view a list of samples the instrument has not yet proc...
- Also called: Worklist, Pending List, Work Order.
- Pericardial Fluid
- Fluid taken from the chest cavity around the heart.
- Peritoneal Fluid
- Fluid taken from the abdominal cavity.
- pg
- Picogram, a unit of gravimetric measure equal to one trillionth of a gram.
- Photometric Measurement
- A process where a beam of white light from an incandescent lamp goes through an optical filter an...
- Pleural Fluid
- Fluid taken from the chest cavity around the lungs.
- Plt Histogram
- The portion of the Plt distribution curve between 0 fL and 36 fL
- PLT (Platelet)
- Platelet count results from the CBC analysis. The system derives this parameter from the Plt hist...
- Positive Identifier
- An identifier that is linked irrevocably to the date and time of instrument analysis on a sample ...
- Precision
- A measure of the ability of the instrument to reproduce similar results when a sample is run repe...
- Also called: reproducibility.
- Precision Test
- The precision test is performed as part of the automatic startup cycles. If any test value exceed...
- Predilute
- Diltion of a sample prior to analysis on the analyzer. The Predilute mode on the LH 700 Series ma...
- Primary Mode
- The closed-vial method of running a sample. You place a closed tube sample in a cassette and plac...
- Also called: Automatic aspiration mode, closed-vial mode, automatic sampling mode.
- Print Profile
- A set of characteristics that define what you want printed and transmitted for sample runs. You c...
- Also called: Reports, Reporting Options
- psi
- Pounds per square inch, a unit of pressure measurement.
- QC (Quality Control)
- A comprehensive set of procedures your laboratory uses to ensure that the instrument is working a...
- Ramp Test
- The ramp test is performed as part of the automatic startup cycles. If any test value exceeds the...
- RBC (Red Blood Cell)
- Red Blood Cell count results from the CBC analysis. This parameter is measured directly using the...
- RBC Histogram
- Misleading results can occur if you estimate the number of cells from the distribution curves bec...
- An RBC distribution curve. The normal curve ranges from 36 to 360 fL. The display starts at 24 fL.
- RDW (Red Distribution Width)
- The size distribution spread of the erythrocyte population derived from the RBC histogram. Expres...
- Record
- A collection of related information, for example, sample identification and results information, ...
- Reflex Manager List
- A list automatically generated by the Workstation based on sample result criteria set up by your ...
- Reportable range
- This range represents the clinical limits of values that have been tested and found to be accurat...
- Reproducibility
- A procedure to check that the system gives consistent results (within established limits) every t...
- Reset button, Analyzer
- If used when the unit is not cycling, data from the last sample in the Analyzer memory is erased.
- If used when the unit is cycling, any data in the Analyzer buffer that has not been transmitted t...
- RET # (Reticulocytes)
- This field and parameter label disappear if the parameter was excluded as part of parameter selec...
- A computed value that represents the absolute number of reticulocytes.
- The sample’s RBC must be known to calculate this value.
- RET % (Reticulocytes)
- Reticulocyte count results from Retic analysis of samples or controls. The percent is measured di...
- Rili-BÄK
- Richtlinien der Bundesarztekammer – Guidelines of the Federal Chamber of Physicians.
- Sample ID (SID)
- One of two possible positive identifiers for sample analysis. The other possible positive identif...
- For Automatic aspiration mode, this identifier is read from the bar-code label of the sample. For...
- Screen Saver
- The Workstation has a screen saver that appears when you are not interacting with the Workstation...
- The screen returns to its normal view when the Workstation receives sample data or when you inter...
- SD (Standard Deviation)
- A measure of deviation from the mean.
- Secondary Mode
- The open-vial method of running a sample. You immerse the aspirator tip in the sample. The instru...
- Also called: open-vial mode, secondary mode, manual sampling mode.
- Select
- When an item is selected, a visual cue is present. For example, appears in a field.
- Serous Fluid
- A general term that describes peritoneal, pleural, and pericardial fluids as a group.
- Sequence Number
- The Workstation considers this field an optional identifier you can use for a sample.
- The Workstation assigns a sequence number to a sample when a sample request is added to the ToDo ...
- Shift
- Consecutive values that abruptly move from one side of the mean to the other, then maintain a con...
- A scheduled period of work.
- SID (Sample ID)
- One of two possible positive identifiers for sample analysis. The other possible positive identif...
- Standard Deviation (SD)
- A measure of deviation from the mean.
- Status Bar
- A horizontal bar that appears toward the bottom of the screen. It displays short instructions tha...
- Stop Bit
- A computer code that indicates the end of a character.
- Suspect Messages
- Suspect messages appear in a separate area of the screen display, printout, and host transmission...
- Sweep Flow
- A steady stream of diluent that flows behind the RBC aperture during sensing periods to keep RBCs...
- Synovial Fluid
- Joint fluid.
- Test (Modes)
- The LH 700 Series has five different test modes. The test mode determines how the LH 700 Series p...
- ToDo List
- A feature of the Workstation that lets you view a list of samples the instrument has not yet proc...
- Also called: Worklist, Pending List, Work Order.
- Toolbar
- A group of graphic buttons on a Workstation window. You can select a button on a Toolbar to quick...
- Trend
- Values that continue to increase or decrease gradually over a period of time. Contrast with shift.
- Tube Adapters
- Special holders that enable small tubes to fit in standard cassettes. Two sizes of gray sleeve ad...
- UL
- Underwriters Laboratory.
- User Name
- A 16-character identifier that uniquely identifies the processor of the samples. Also called: ope...
- UWBC
- UWBC (Uncorrected WBC)
- This field and parameter label do not appear on any screen if the parameter was excluded as part ...
- Uncorrected WBC, labeled "UWBC" is measured directly using the Coulter Principle.
- UWBC cannot be edited. The uncorrected WBC is displayed on the CBC Data tab.
- Vac
- Volts of alternating current.
- VCS (Volume, Conductivity and Scatter)
- A flow cytometry technology applied in hematology to enhance WBC subpopulation classification and...
- Voting
- After the computer corrects for coincidence, it compares the three count periods. Voting may occu...
- W
- Watt, a unit of power.
- WBC (White Blood Cell)
- White Blood Cell count results from the CBC analysis. The WBC count is adjusted for interfering s...
- This parameter is measured directly using the Coulter Principle.
- Wettability
- The ability of any solid surface to be wetted when in contact with a liquid. Wettability affects ...
- Window
- A rectangular area on your computer screen that enables you to work with an application.
- Windows 2000 Workstation Operating System
- The Microsoft operating system for your computer.
- Worklist
- A feature of the Workstation that lets you view a list of samples the instrument has not yet proc...
- Also called: Worklist, Pending List, Work Order.
- Work Order
- A feature of the Workstation that lets you view a list of samples the instrument has not yet proc...
- Also called: Worklist, Pending List, Work Order.
- Workstation
- The computer hardware and software that controls instrument operation; displays, stores, and reca...
- Also called: ADMS, DMS, LH Workstation.
- XB Analysis
- A method of quality control that automatically compares patient red blood cell indices (MCV, MCH ...
- XM Analysis
- XM is a method of quality control that uses an Exponentially Weighted Moving Average (EWMA) of CB...
- INDEX
- BECKMAN COULTER, INC. CUSTOMER END USER LICENSE AGREEMENT
- TRADEMARKS
- Beckman Coulter, the stylized logo, and COULTER are trademarks of Beckman Coulter, Inc. and are r...
- All other trademarks, service marks, products, or services are trademarks or registered trademark...
- Find us on the World Wide Web at: www.beckmancoulter.com
- Made in USA
- COULTER LH 700 SERIES SYSTEM HARD-COPY DOCUMENTATION
- s Host Transmission PN 4277303
- s Reference PN 4277248
- s Operator’s Guide PN 4277249
- s Special Procedures and Troubleshooting PN 4277250
- s SlideMaker Operator’s Guide PN 4277299
- s SlideStainer Operator’s Guide PN 4277300
- s Body Fluids Instructions for Use PN 731113
- s Hematology Tube List PN A70017
- Come visit us at www.beckmancoulter.com
PN 4277248DD
GLOSSARY-7
GLOSSARY
Histograms Graphic representations of cell frequency vs. size. Histograms provide
information about leukocyte, erythrocyte and thrombocyte frequency and their
distribution about the mean. They also might show the presence of
subpopulations.
Hz Hertz, a unit of frequency.
i.d. Inside diameter.
IEC International Electrtechnical Commission.
Information System Any host or laboratory computer system.
Instrument The Analyzer and Diluter portion of the LH 700 Series.
IQAP
(Interlaboratory
Quality Assurance
Program)
Beckman Coulter Inc. provides this program, which statistically compares your
5C Cell control and Retic-C Cell control data to a group of other laboratories'
control recovery data with the same control lot number.
IRF (Immature
Reticulocyte
Fraction)
This parameter is derived from VCS technology.
IRF = High Light Scatter Reticulocytes / Total reticulocytes
L Liter, a unit of volumetric measurement.
Lab Administrator A person at your location who can access the full set of Workstation functions.
The lab administrator creates user names and assigns access levels. A specific
user name and password is assigned to the lab administrator in your laboratory.
Laser (Light
Amplification by
Stimulated Emission
of Radiation)
The instrument uses a laser for WBC Diff and Retic analysis.
Last Name Referes to the patient’s last name. The Workstation considers this field an
optional identifier you can use for a sample. This field appears blank unless you
provide the information when you add a sample request to the ToDo list or edit
a sample result on the Edit Sample window.
Levey-Jennings
Control Chart
A commonly used control procedure in which control measurements are plotted
directly on a control chart with limit lines drawn as mean plus or minus
expected ranges.
Linearity The ability of an instrument to recover expected results (reference values or
calculated values) for such parameters as WBC, RBC, Hgb and Plt at varying
levels of concentration of these parameters within specified limits.
Beckman Coulter Inc. provides the LIN-C linearity control for your
convenience. Use it according to the instructions on its package insert.
Lot Number An identifier assigned by a manufacturer to identify a control, reagent or
calibrator.
LY #(Lymphocytes) A WBC differential parameter result from the Diff sample analysis. The number
(#) is computed from the WBC count and the LY %.