Instruction manual

Software Reference

2.5 WINDOW MENU

This menu allows you to specify what type of data you want to display.

Band Table

A band table is a resizable and scrollable window displaying data about each marked band. This selection

is a toggle function for hiding or displaying band tables.

The different band tables are available by clicking on the word “Mode” in any displayed band table. Note the

following:

• This is selected from the upper left corner of any band table. It is not a menu bar item.

• Selecting a mode does not display that band table; you must also have Band Table selected.

Band Location

The location of a band is measured in pixels on an X/Y axis. This table displays the Y-axis value of the

center of each marked band (as indicated by its cross-hair). Since the band is associated with a lane,

its X-axis value is not important. The corrected value refers to the value assigned by the software after

it has accounted for any curving up or down of the gel.

Band Location/Total Gray

The Band Location values are the corrected values from the Band Location table. The Total Gray value

for a band is the total intensity for all pixels in that band.

Total Gray

These values are the same as those presented in the Band Location/Total Gray table.

Peak Gray

This is the largest gray value (up to a value of 65,000) in the selected band. (This value does not nec-

essarily correspond to the center of the cross-hair.)

Percent luminance of Selected Bands

This presents the relative intensity (percentage of luminance) for each band in a selected lane. The

total for all bands in a lane is 100%. (The data in this table is similar to that in the Total Gray band

table, which presents the data as the total intensity for all pixels in a selected band.)

Quantity

This is the concentration (in picomols) of the marked bands.

Description

This is any text you might choose to enter. To enter text from the band table, double click on the desired

band’s field or on the desired band in the image. Either method displays the Information about

Selected Band window where you can enter the description.