User Manual

Tris/Acetic Acid/EDTA (TAE) 40 mM Tris,

20 mM acetic acid,

1 mM EDTA,

pH 8.0

Premixed buffers are available (see Section 4.) Dilute the premixed 10x buffers (10x

Tris/Glycine/SDS, 10x Tris/Glycine, 10x Tris/Tricine/SDS, 10x TBE, and 50x TAE) to a

working concentration of 1x. Mix one liter of the premixed 10x buffer with 9 liters of distilled

water to prepare 10 liters of running buffer.

2.2 Preparing the Criterion Precast Gel

All gels in a single electrophoresis experiment must be of the same gel type, with the

same buffer and the same % acrylamide.

Complete instructions for Criterion precast gels

may be ordered using catalog number 345-0000. Instructions are also available on the

internet at www.discover.bio-rad.com in the precast gels literature section (bulletin 4110001).

a. Each Criterion gel is packaged individually in a plastic storage tray. Remove the cover by

gently pulling the square corner tab up and diagonally across the package. Remove the gel

cassette from the package.

b. Remove the comb and gently rinse the wells with dd H

0 or running buffer.

c. Remove the white tape from the bottom of the cassette by pulling the tab across the gel.

2.3 Assembling the Cell

a. Place the Dodeca Cell on a stir plate. Fill the tank half full and drop a 38 mm (1.5") stir bar

into the buffer tank. (This step must be done prior to loading the cassettes into the cell.)

Inserting Criterion gel cassette into tank.

b. Insert each Criterion gel into one of the slots in the Criterion Dodeca Cell. Ensure that the

integral buffer chamber is facing the chiller hook-ups. Gel cassette orientation is indicated

with a label on the tank.

c. Fill the upper buffer chamber of each cassette with approximately 60 ml buffer.

If running gels with different buffers or % acrylamide, the run progress should be monitored closely and gels can be removed as

neccessary.