User Manual

17
3.5. 2-D Gel Staining
Oriole fluorescent gel stain is ideal for staining 2-D
polyacrylamide gels. Clear background-free images
are obtained without interference from CHAPS, carrier
ampholytes, or other components of the first-dimension
separation.
Fig. 5. 2-D gel stained with Oriole stain. E. coli protein
(40 μg) was run on an 11 cm pH 5–8 ReadyStrip
™
IPG strip for the
first dimension and Criterion Tris-HCl 8–16% gel for the second
dimension. The gel was stained with Oriole stain and was imaged
on the Molecular Imager VersaDoc MP 4000 imaging system. The
resulting image was inverted.